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The map label for this gene is lepA [H]

Identifier: 91784709

GI number: 91784709

Start: 3696773

End: 3698566

Strand: Reverse

Name: lepA [H]

Synonym: Bxe_A1083

Alternate gene names: 91784709

Gene position: 3698566-3696773 (Counterclockwise)

Preceding gene: 91784710

Following gene: 91784708

Centisome position: 75.55

GC content: 59.81

Gene sequence:

>1794_bases
ATGGATCATATTCGTAACTTCTCGATCATTGCGCACATCGACCATGGCAAGTCGACGCTCGCCGATCGCATTATCCAGAT
CTGCGGCGGCCTGTCCGACCGCGAGATGGAATCCCAAGTGCTCGATTCGATGGACCTCGAACGCGAGCGCGGCATCACCA
TCAAGGCACAAACCGCCGCACTGACATACAAGGCCCGTGATGGGCAAGTCTACAACCTGAACATGATCGACACGCCGGGC
CACGTCGACTTCTCGTACGAAGTCAGCCGCTCGCTGTCCGCATGCGAAGGCGCGCTGCTGGTCGTCGACGCGAGCCAGGG
CGTCGAGGCGCAAACCGTGGCCAACTGCTACACGGCAATCGAACTCGGCGTCGACGTGATTCCGGTGCTCAACAAGATCG
ACCTGCCGGCGGCCAACCCCGAAAATGCGATCGCCGAAATCGAAGACGTGATCGGCATCGACGCAACCGACGCCACGCGC
TGCAGCGCGAAGACGGGCCTCGGCGTCGAAGACGTGCTCGAAGCATTGGTCGCCAAAGTGCCGCCGCCCAAGGGCGATCC
GGAAGCGCCGTTGCAGGCGCTGATCATCGATTCGTGGTTCGACAACTACGTCGGCGTCGTGATGCTGGTGCGTATCGTCA
ACGGCACGCTGCGTCCCAAGGACAAGATCCGCATGATGGCGACCGGCGCGCAGTATCCGGTCGAACATATCGGCGTGTTC
ACGCCGAAGTCGAAGAATCTCGAATCGCTGTCGGCCGGGCAGGTGGGCTTCATCATCGCCGGCATCAAGGAACTGGCCGC
GGCGAAGGTGGGCGACACCGTCACGCTGGTGAACCGTCCGGCCGCCGAGCCGCTGCCGGGCTTCAAGGAAGTGAAGCCGC
AGGTGTTCGCCGGTCTCTATCCGGTCGAAGCGAACCAGTACGACGCGCTGCGCGATTCGCTGGAAAAGCTGAAGCTGAAC
GACGCCTCGCTGATGTACGAGCCGGAAGTGTCGCAGGCGCTCGGTTTCGGTTTCCGTTGCGGCTTCCTCGGTCTGCTGCA
CATGGAGATCGTGCAGGAACGTCTCGAACGCGAATTCGACATGGACCTGATCACCACGGCGCCGACCGTGGTGTACGAAG
TCCTGCAGCGCGACGGCACGACCATCATGGTCGAGAATCCGGCCAAGATGCCGGAACCGTCGAAGATCGAAGAAGTGCGC
GAGCCGATCGTCACCGTGAATCTGTACATGCCGCAAGACTATGTCGGCTCGGTGATCACGCTGTGCACGCAAAAGCGCGG
CACGCAGATCAACATGCAGTATCACGGCCGTCAGGTGCAGTTGACCTACGAGATCCCGATGGGCGAAGTCGTGCTCGATT
TCTTCGACCGTCTGAAGTCGATCTCGCGCGGCTACGCGTCGATGGATTACGAGTTCAAGGAATATCGCGCGGCAGACGTG
GTGAAGGTCGACATGCTGATCAACGGCGACAAGGTCGACGCGCTGTCGGTCATCGTGCACCGGTCGCAAAGCCAGTATCG
CGGCCGCGAAGTGGCGTCGAAAATGCGCGAATTGATTCCGCGTCAGATGTACGACGTGGCGATTCAGGCCACCATCGGCG
CGAACATTATTGCGCGCGAAAACATTAAAGCGTTGCGTAAGAACGTGCTGGCAAAATGCTACGGCGGCGACATTTCTCGT
AAGAAGAAGCTGCTGGAAAAGCAAAAGGCAGGCAAAAAGCGAATGAAGCAGGTGGGATCGGTCGAGATTCCGCAAGAGGC
TTTCCTCGCCATTCTGCGTGTCGAAGACAAATAA

Upstream 100 bases:

>100_bases
GGCGATAGCCTTTTCGGCTAAAATAGATAGGTTTTTCACCTACTTACAAGGCGTGCTCCGCTATTGTCCGAGCGCGCCTT
TTTCGCTTGATCGGTACTGA

Downstream 100 bases:

>100_bases
GACGAACAACGGAACCCTATGAATTTTGCGCTGATTCTTTTTGTGCTCGTCATTTTGACGGGCGTCGCATGGGTCGCAGA
CAAACTGGTTTTCATGCCGC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMESQVLDSMDLERERGITIKAQTAALTYKARDGQVYNLNMIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVDVIPVLNKIDLPAANPENAIAEIEDVIGIDATDATR
CSAKTGLGVEDVLEALVAKVPPPKGDPEAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIRMMATGAQYPVEHIGVF
TPKSKNLESLSAGQVGFIIAGIKELAAAKVGDTVTLVNRPAAEPLPGFKEVKPQVFAGLYPVEANQYDALRDSLEKLKLN
DASLMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVLQRDGTTIMVENPAKMPEPSKIEEVR
EPIVTVNLYMPQDYVGSVITLCTQKRGTQINMQYHGRQVQLTYEIPMGEVVLDFFDRLKSISRGYASMDYEFKEYRAADV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVASKMRELIPRQMYDVAIQATIGANIIARENIKALRKNVLAKCYGGDISR
KKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK

Sequences:

>Translated_597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMESQVLDSMDLERERGITIKAQTAALTYKARDGQVYNLNMIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVDVIPVLNKIDLPAANPENAIAEIEDVIGIDATDATR
CSAKTGLGVEDVLEALVAKVPPPKGDPEAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIRMMATGAQYPVEHIGVF
TPKSKNLESLSAGQVGFIIAGIKELAAAKVGDTVTLVNRPAAEPLPGFKEVKPQVFAGLYPVEANQYDALRDSLEKLKLN
DASLMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVLQRDGTTIMVENPAKMPEPSKIEEVR
EPIVTVNLYMPQDYVGSVITLCTQKRGTQINMQYHGRQVQLTYEIPMGEVVLDFFDRLKSISRGYASMDYEFKEYRAADV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVASKMRELIPRQMYDVAIQATIGANIIARENIKALRKNVLAKCYGGDISR
KKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK
>Mature_597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMESQVLDSMDLERERGITIKAQTAALTYKARDGQVYNLNMIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVDVIPVLNKIDLPAANPENAIAEIEDVIGIDATDATR
CSAKTGLGVEDVLEALVAKVPPPKGDPEAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIRMMATGAQYPVEHIGVF
TPKSKNLESLSAGQVGFIIAGIKELAAAKVGDTVTLVNRPAAEPLPGFKEVKPQVFAGLYPVEANQYDALRDSLEKLKLN
DASLMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVLQRDGTTIMVENPAKMPEPSKIEEVR
EPIVTVNLYMPQDYVGSVITLCTQKRGTQINMQYHGRQVQLTYEIPMGEVVLDFFDRLKSISRGYASMDYEFKEYRAADV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVASKMRELIPRQMYDVAIQATIGANIIARENIKALRKNVLAKCYGGDISR
KKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=48.1788079470199, Blast_Score=610, Evalue=1e-175,
Organism=Homo sapiens, GI18390331, Length=160, Percent_Identity=38.75, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=44.3609022556391, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=45.6375838926174, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI19923640, Length=149, Percent_Identity=45.6375838926174, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI25306287, Length=149, Percent_Identity=45.6375838926174, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=40.9722222222222, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=42.7272727272727, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=42.7272727272727, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=42.7272727272727, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=32.3308270676692, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=32.3308270676692, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI53729339, Length=218, Percent_Identity=31.1926605504587, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI53729337, Length=218, Percent_Identity=31.1926605504587, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=70.5387205387205, Blast_Score=856, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=506, Percent_Identity=29.2490118577075, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=36.7741935483871, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.0512820512821, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789559, Length=228, Percent_Identity=28.0701754385965, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=39.3790849673203, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17556745, Length=467, Percent_Identity=25.2676659528908, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17533571, Length=161, Percent_Identity=39.1304347826087, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=37.1794871794872, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=38.8059701492537, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=38.8059701492537, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.0827067669173, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25141371, Length=312, Percent_Identity=27.2435897435897, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=47.1760797342193, Blast_Score=566, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=35.1648351648352, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=40.2777777777778, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=40.2777777777778, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=40.8695652173913, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=38.1944444444444, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325337, Length=156, Percent_Identity=32.0512820512821, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319594, Length=156, Percent_Identity=32.0512820512821, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=282, Percent_Identity=26.5957446808511, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=44.8504983388704, Blast_Score=542, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=37.888198757764, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=42.3357664233577, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=36.4864864864865, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=36.4864864864865, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=36.4864864864865, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=33.8345864661654, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28572034, Length=219, Percent_Identity=29.2237442922374, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66052; Mature: 66052

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMESQVLDSMDLERERGITIKAQTAA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCEEEEEEEE
LTYKARDGQVYNLNMIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
EEEEECCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
ELGVDVIPVLNKIDLPAANPENAIAEIEDVIGIDATDATRCSAKTGLGVEDVLEALVAKV
HHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PPPKGDPEAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIRMMATGAQYPVEHIGVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCHHHCEEE
TPKSKNLESLSAGQVGFIIAGIKELAAAKVGDTVTLVNRPAAEPLPGFKEVKPQVFAGLY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHCCHHHHHCCC
PVEANQYDALRDSLEKLKLNDASLMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
MDLITTAPTVVYEVLQRDGTTIMVENPAKMPEPSKIEEVREPIVTVNLYMPQDYVGSVIT
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCEEEEEEECCHHHHHHHHH
LCTQKRGTQINMQYHGRQVQLTYEIPMGEVVLDFFDRLKSISRGYASMDYEFKEYRAADV
HHHHCCCCEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCE
VKVDMLINGDKVDALSVIVHRSQSQYRGREVASKMRELIPRQMYDVAIQATIGANIIARE
EEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHH
NIKALRKNVLAKCYGGDISRKKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC
>Mature Secondary Structure
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMESQVLDSMDLERERGITIKAQTAA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCEEEEEEEE
LTYKARDGQVYNLNMIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
EEEEECCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
ELGVDVIPVLNKIDLPAANPENAIAEIEDVIGIDATDATRCSAKTGLGVEDVLEALVAKV
HHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PPPKGDPEAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIRMMATGAQYPVEHIGVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCHHHCEEE
TPKSKNLESLSAGQVGFIIAGIKELAAAKVGDTVTLVNRPAAEPLPGFKEVKPQVFAGLY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHCCHHHHHCCC
PVEANQYDALRDSLEKLKLNDASLMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
MDLITTAPTVVYEVLQRDGTTIMVENPAKMPEPSKIEEVREPIVTVNLYMPQDYVGSVIT
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCEEEEEEECCHHHHHHHHH
LCTQKRGTQINMQYHGRQVQLTYEIPMGEVVLDFFDRLKSISRGYASMDYEFKEYRAADV
HHHHCCCCEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCE
VKVDMLINGDKVDALSVIVHRSQSQYRGREVASKMRELIPRQMYDVAIQATIGANIIARE
EEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHH
NIKALRKNVLAKCYGGDISRKKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA