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The map label for this gene is amnB [H]

Identifier: 91784649

GI number: 91784649

Start: 3639253

End: 3640167

Strand: Reverse

Name: amnB [H]

Synonym: Bxe_A1145

Alternate gene names: 91784649

Gene position: 3640167-3639253 (Counterclockwise)

Preceding gene: 91784650

Following gene: 91784648

Centisome position: 74.35

GC content: 59.67

Gene sequence:

>915_bases
ATGAACGAAGGCAAGATTCTGTCCGGCCTCTTGGCGCCCCATCCGCCGCATCTGGTCTATGGGGAGAACCCGCCTCAGAA
CGAACCGAGGTCGCAAGGCGGCTGGGAACCGCTGCGCTGGGCCTACGATCAAGTGCGCGAGAACATCGAGCATCTGAAGC
CCGATGTTCTGATCGTCCATTCCCCGCACTGGATCACTCAGGTGGGCCATCATTTCCTGGGCGTACCTCGGCTTACGGGG
CGCTCGGTCGATCCCATCTTTCCGAATCTGTTCCGCTACGACTTCGGCATGAACGTCGATGTGGAACTGGCCGAGGCCTG
CTGCGAGGAAGCAACCAAGCTGGGTCTGGTCAGCAAGATGATGCGCAACCCGAGCTTCCGCGTGGACTACGGGACCATCA
CCACGCTGCACATGGTCCGCCCGCAATGGGATATCCCCGTGGTGGGGATCTCGGCCAACAACACGCCGTACTACCTCAAC
ACCAAAGAGGGGCTGGGCGAGATGGACCTTCTCGGTCGTGCCACGCGAGAAGCTATTCGGAAGACGGGGCGGCGTGCAGT
CCTTCTGGCGAGCAATACGCTGTCGCACTGGCACTTCCATGAGGAGCCTGCGCTGCCTGAGGACATGTCCAAAGAGCACC
CGGAGCGCTACGACGGCTACTTGTGGGATGTACGCATGATCGAACTCATGCGCAAGGGCCACATGAAGGAAGTCTTCGAA
CTGCTCCCTCAGTTCATCGAAGAAGCCTTTGCTGAAATCAAGTCCGGTGCATTCACTTGGATGCACGCAGCGATGGGTTA
TCCCGAACTGGCCGGCAAGCTACATGGCTACGGCACCGTCATCGGCACTGGCAACGCGGTGATCGAGTGGAACCTTGAGG
ATGTCGGCCTTTCCCGTTTGGAGTCCGTGGCGTAA

Upstream 100 bases:

>100_bases
AGCTCGCTCCACCCGCCAGAACCTTCACGGTCGTGAAGTCCCTCCCGCTGGGTGCTGCGGTGGAAATCGAGTTTCAGTTG
TCAATTTGAGGAGAACTGCT

Downstream 100 bases:

>100_bases
GCCGCCTGAGTCCACCATAAGCATAAGAAGCAGGAGACTTCAACATGACCATCGTTTCTGCATTCCTCCTCCCCGGCAGC
CCACTGCCGCAGTTGCGGCC

Product: 2-aminophenol 1,6-dioxygenase, beta subunit

Products: 2-hydroxy-5-carboxymethylmuconate semialdehyde

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNEGKILSGLLAPHPPHLVYGENPPQNEPRSQGGWEPLRWAYDQVRENIEHLKPDVLIVHSPHWITQVGHHFLGVPRLTG
RSVDPIFPNLFRYDFGMNVDVELAEACCEEATKLGLVSKMMRNPSFRVDYGTITTLHMVRPQWDIPVVGISANNTPYYLN
TKEGLGEMDLLGRATREAIRKTGRRAVLLASNTLSHWHFHEEPALPEDMSKEHPERYDGYLWDVRMIELMRKGHMKEVFE
LLPQFIEEAFAEIKSGAFTWMHAAMGYPELAGKLHGYGTVIGTGNAVIEWNLEDVGLSRLESVA

Sequences:

>Translated_304_residues
MNEGKILSGLLAPHPPHLVYGENPPQNEPRSQGGWEPLRWAYDQVRENIEHLKPDVLIVHSPHWITQVGHHFLGVPRLTG
RSVDPIFPNLFRYDFGMNVDVELAEACCEEATKLGLVSKMMRNPSFRVDYGTITTLHMVRPQWDIPVVGISANNTPYYLN
TKEGLGEMDLLGRATREAIRKTGRRAVLLASNTLSHWHFHEEPALPEDMSKEHPERYDGYLWDVRMIELMRKGHMKEVFE
LLPQFIEEAFAEIKSGAFTWMHAAMGYPELAGKLHGYGTVIGTGNAVIEWNLEDVGLSRLESVA
>Mature_304_residues
MNEGKILSGLLAPHPPHLVYGENPPQNEPRSQGGWEPLRWAYDQVRENIEHLKPDVLIVHSPHWITQVGHHFLGVPRLTG
RSVDPIFPNLFRYDFGMNVDVELAEACCEEATKLGLVSKMMRNPSFRVDYGTITTLHMVRPQWDIPVVGISANNTPYYLN
TKEGLGEMDLLGRATREAIRKTGRRAVLLASNTLSHWHFHEEPALPEDMSKEHPERYDGYLWDVRMIELMRKGHMKEVFE
LLPQFIEEAFAEIKSGAFTWMHAAMGYPELAGKLHGYGTVIGTGNAVIEWNLEDVGLSRLESVA

Specific function: Component of the 2-aminophenol 1,6-dioxygenase complex that catalyzes the ring fission of 2-aminophenol to produce 2- aminomuconic 6-semialdehyde. AmnB seems to be the catalytic subunit of the complex. The enzyme is also active toward 2-amino- p-cresol, 6

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ligB/mhpB extradiol dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: 1.13.11.15

Molecular weight: Translated: 34514; Mature: 34514

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEGKILSGLLAPHPPHLVYGENPPQNEPRSQGGWEPLRWAYDQVRENIEHLKPDVLIVH
CCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
SPHWITQVGHHFLGVPRLTGRSVDPIFPNLFRYDFGMNVDVELAEACCEEATKLGLVSKM
CCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
MRNPSFRVDYGTITTLHMVRPQWDIPVVGISANNTPYYLNTKEGLGEMDLLGRATREAIR
HCCCCCEEECCCEEEEEEECCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHH
KTGRRAVLLASNTLSHWHFHEEPALPEDMSKEHPERYDGYLWDVRMIELMRKGHMKEVFE
HCCCEEEEEECCCHHHCCCCCCCCCCHHHHHCCCHHHCCEEHHHHHHHHHHCCCHHHHHH
LLPQFIEEAFAEIKSGAFTWMHAAMGYPELAGKLHGYGTVIGTGNAVIEWNLEDVGLSRL
HHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCCEEEEECCCEEEEECHHHHHHHHH
ESVA
HHCC
>Mature Secondary Structure
MNEGKILSGLLAPHPPHLVYGENPPQNEPRSQGGWEPLRWAYDQVRENIEHLKPDVLIVH
CCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
SPHWITQVGHHFLGVPRLTGRSVDPIFPNLFRYDFGMNVDVELAEACCEEATKLGLVSKM
CCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
MRNPSFRVDYGTITTLHMVRPQWDIPVVGISANNTPYYLNTKEGLGEMDLLGRATREAIR
HCCCCCEEECCCEEEEEEECCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHH
KTGRRAVLLASNTLSHWHFHEEPALPEDMSKEHPERYDGYLWDVRMIELMRKGHMKEVFE
HCCCEEEEEECCCHHHCCCCCCCCCCHHHHHCCCHHHCCEEHHHHHHHHHHCCCHHHHHH
LLPQFIEEAFAEIKSGAFTWMHAAMGYPELAGKLHGYGTVIGTGNAVIEWNLEDVGLSRL
HHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCCEEEEECCCEEEEECHHHHHHHHH
ESVA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3,4-dihydroxyphenylacetate; O2

Specific reaction: 3,4-dihydroxyphenylacetate + O2 = 2-hydroxy-5-carboxymethylmuconate semialdehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9169437; 11081795 [H]