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The map label for this gene is otsB [H]

Identifier: 91784558

GI number: 91784558

Start: 3550403

End: 3551212

Strand: Reverse

Name: otsB [H]

Synonym: Bxe_A1241

Alternate gene names: 91784558

Gene position: 3551212-3550403 (Counterclockwise)

Preceding gene: 91784562

Following gene: 91784557

Centisome position: 72.54

GC content: 65.56

Gene sequence:

>810_bases
ATGCGCGGTAAGATGCGCACACTTTTCCGGCCGCACAAGGCTGAAAGGCATAACCAACCGATGCAAGCACTCCCGGCTGT
TCTGTCTCCAGGCGAGACGGCATTTTTCTTCGATTTCGACGGCACACTCGTCGAACTCGCTCCCACGCCGGACGGCGTGC
TGGTGCAACCTCGCGTGATCGACTTGCTGAGCGAGTTGCGCAGCCTGACGAACGGTGCCGTCGCGGTGGTGTCGGGGCGT
GGGATCGACAGCATCGACAGTTTTCTGGGTATTCCCGATCTGCCGATTGCCGGTCTGCACGGCGCCGAACGGCGTGACGC
GAACGGCGACACGCAACGCATCGGCTTTCATGACGAGCGGCTCCTGCGCATGGAGCAGGTGCTCGCGCAAGTGGTCAACG
AAAACCCCGGCATGCTGCTCGAGATCAAGGGCGCGGCCGTGGCGCTGCACTACCGGAATGCGCCGGACCGCGAGCCGGTG
GCGCGCGAGGCAACCAGCCGGCTGGTCGCCGATTACGCCGGCGCCTACGTGTTGCAGCCGGGCAAGATGGTCTACGAAAT
CAAACCGAAAGATGTCGACAAGGGCCGCGCGCTGCGCGCCTTTCTCGACGAGCCGCCTTTCGTCGGCCGCCAGCCGGTCT
TTGCCGGCGACGATCTGACCGACGAGAAAGGCTTCGCCGTCGTCAACGAACGCGGCGGGCTGTCGATCAAGGTCGGCCCG
GGCGAGACGATGGCGCGAACGCGCATCGACTCGGTCCCGGCGCTGCTCGACTGGCTGGCATCGATCGTCGCGGCCGCGCG
CGCGGCATGA

Upstream 100 bases:

>100_bases
AAGCTTGATTGTTGTCATTTTGAACGCAATCGACGGCGGTGCATGCGGGTGGCGCCCGGCTGGGCTTGTGTCCAGCCTTA
CTTTCTCGAAAAATTGACGG

Downstream 100 bases:

>100_bases
TGGCCATGCCGCATTGCTCAGAACCTATAACAAACCGCATCGGGAATCGTGCTTCATGGGCCGACTGATCATCGTATCGA
ACCGGGTTGCGCCGATCTCG

Product: trehalose-phosphatase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRGKMRTLFRPHKAERHNQPMQALPAVLSPGETAFFFDFDGTLVELAPTPDGVLVQPRVIDLLSELRSLTNGAVAVVSGR
GIDSIDSFLGIPDLPIAGLHGAERRDANGDTQRIGFHDERLLRMEQVLAQVVNENPGMLLEIKGAAVALHYRNAPDREPV
AREATSRLVADYAGAYVLQPGKMVYEIKPKDVDKGRALRAFLDEPPFVGRQPVFAGDDLTDEKGFAVVNERGGLSIKVGP
GETMARTRIDSVPALLDWLASIVAAARAA

Sequences:

>Translated_269_residues
MRGKMRTLFRPHKAERHNQPMQALPAVLSPGETAFFFDFDGTLVELAPTPDGVLVQPRVIDLLSELRSLTNGAVAVVSGR
GIDSIDSFLGIPDLPIAGLHGAERRDANGDTQRIGFHDERLLRMEQVLAQVVNENPGMLLEIKGAAVALHYRNAPDREPV
AREATSRLVADYAGAYVLQPGKMVYEIKPKDVDKGRALRAFLDEPPFVGRQPVFAGDDLTDEKGFAVVNERGGLSIKVGP
GETMARTRIDSVPALLDWLASIVAAARAA
>Mature_269_residues
MRGKMRTLFRPHKAERHNQPMQALPAVLSPGETAFFFDFDGTLVELAPTPDGVLVQPRVIDLLSELRSLTNGAVAVVSGR
GIDSIDSFLGIPDLPIAGLHGAERRDANGDTQRIGFHDERLLRMEQVLAQVVNENPGMLLEIKGAAVALHYRNAPDREPV
AREATSRLVADYAGAYVLQPGKMVYEIKPKDVDKGRALRAFLDEPPFVGRQPVFAGDDLTDEKGFAVVNERGGLSIKVGP
GETMARTRIDSVPALLDWLASIVAAARAA

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=248, Percent_Identity=43.1451612903226, Blast_Score=199, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320279, Length=251, Percent_Identity=25.4980079681275, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920676, Length=240, Percent_Identity=28.75, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 29181; Mature: 29181

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGKMRTLFRPHKAERHNQPMQALPAVLSPGETAFFFDFDGTLVELAPTPDGVLVQPRVI
CCCCCCHHHCCCHHHHCCCCHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCEEECHHHH
DLLSELRSLTNGAVAVVSGRGIDSIDSFLGIPDLPIAGLHGAERRDANGDTQRIGFHDER
HHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCHHH
LLRMEQVLAQVVNENPGMLLEIKGAAVALHYRNAPDREPVAREATSRLVADYAGAYVLQP
HHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECC
GKMVYEIKPKDVDKGRALRAFLDEPPFVGRQPVFAGDDLTDEKGFAVVNERGGLSIKVGP
CCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCEECCCCCCCCCCEEEEECCCCEEEEECC
GETMARTRIDSVPALLDWLASIVAAARAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRGKMRTLFRPHKAERHNQPMQALPAVLSPGETAFFFDFDGTLVELAPTPDGVLVQPRVI
CCCCCCHHHCCCHHHHCCCCHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCEEECHHHH
DLLSELRSLTNGAVAVVSGRGIDSIDSFLGIPDLPIAGLHGAERRDANGDTQRIGFHDER
HHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCHHH
LLRMEQVLAQVVNENPGMLLEIKGAAVALHYRNAPDREPVAREATSRLVADYAGAYVLQP
HHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECC
GKMVYEIKPKDVDKGRALRAFLDEPPFVGRQPVFAGDDLTDEKGFAVVNERGGLSIKVGP
CCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCEECCCCCCCCCCEEEEECCCCEEEEECC
GETMARTRIDSVPALLDWLASIVAAARAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]