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The map label for this gene is gloB [H]

Identifier: 91784546

GI number: 91784546

Start: 3537810

End: 3538601

Strand: Reverse

Name: gloB [H]

Synonym: Bxe_A1253

Alternate gene names: 91784546

Gene position: 3538601-3537810 (Counterclockwise)

Preceding gene: 91784552

Following gene: 91784545

Centisome position: 72.28

GC content: 64.39

Gene sequence:

>792_bases
ATGAATGCGCTCGAGTACGTACCGGTCCCGGCGTTTGATGACAATTACATCTGGGTCGTTTCCGACGGACACTCTGCGGT
AGTCGTCGATCCGGGTGAGGCCGCCCCCGTGCAAGCTTATCTGGCGAAACGGGGTTGGCGGCTGAGCGCTATTTTACTCA
CGCACCATCATCAAGACCACGTCGGCGGGGTGGCCGATCTGCTGAATGGCCAGGCGGTGCCCGTCTACGGCCCCGCCGGT
GAAGCGATCGAGCATCTCACGCACCGCCTGAAAAATGGCGATCACGTGTCGATCGCGGCCCCCGCGCTCGAATTGAGCGT
GCTCGACGTGCCCGGCCATACGAGCGGTCACATTGCCTATTTTCAGGCGGCGGACCCGCGCGGCACGCCGCACGTATTTT
GCGGCGACACGCTGTTCGCCTGCGGTTGCGGCCGGCTGTTCGAAGGCACGCCGAAGCAGATGCTCGCCTCGCTGGATGCG
CTCGCCGCGCTGCCCGGCGCAACCGAGGTGCATTGCGCGCACGAGTACACGCTGTCGAATATCCGCTTCGCGCTCGCCTG
CGAGCCGGCCAACGCCGAACTGCAAGCCTGGCGGGATAAAGCAGGGGAATTGCGCGCGCGCGGCGTGCCGACGCTGCCCA
CCACGATCGCCCACGAACGCGCGGTGAATCCGTTTTTGCGTGCCGGCAATCCGGCAGTGCAGGCAAGCCTGCGGGAGCAG
CTGCACGAAAAGGTGCCCGATCGATTGACGGCCTTCACGTTGATGCGCGAATGGAAAAACCGCTTCCGTTGA

Upstream 100 bases:

>100_bases
GGTCTGCTCCCACTCGAGCACGTAGCGGCCAGGCGGCGAATCGGTCCAGGCGGGCCAGTCTATAATCGCTCGATCAGTCA
TATCAGAGAATGCGTCGCCT

Downstream 100 bases:

>100_bases
CCCGGTCCGGATGAGGGTATTCCTCACGCGAAGAATCGGGAAAATTCGCCAAGCCCAAGATTTACTTGATTTTTTCGTAA
TTTTCCGATTGACGTAAACG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNALEYVPVPAFDDNYIWVVSDGHSAVVVDPGEAAPVQAYLAKRGWRLSAILLTHHHQDHVGGVADLLNGQAVPVYGPAG
EAIEHLTHRLKNGDHVSIAAPALELSVLDVPGHTSGHIAYFQAADPRGTPHVFCGDTLFACGCGRLFEGTPKQMLASLDA
LAALPGATEVHCAHEYTLSNIRFALACEPANAELQAWRDKAGELRARGVPTLPTTIAHERAVNPFLRAGNPAVQASLREQ
LHEKVPDRLTAFTLMREWKNRFR

Sequences:

>Translated_263_residues
MNALEYVPVPAFDDNYIWVVSDGHSAVVVDPGEAAPVQAYLAKRGWRLSAILLTHHHQDHVGGVADLLNGQAVPVYGPAG
EAIEHLTHRLKNGDHVSIAAPALELSVLDVPGHTSGHIAYFQAADPRGTPHVFCGDTLFACGCGRLFEGTPKQMLASLDA
LAALPGATEVHCAHEYTLSNIRFALACEPANAELQAWRDKAGELRARGVPTLPTTIAHERAVNPFLRAGNPAVQASLREQ
LHEKVPDRLTAFTLMREWKNRFR
>Mature_263_residues
MNALEYVPVPAFDDNYIWVVSDGHSAVVVDPGEAAPVQAYLAKRGWRLSAILLTHHHQDHVGGVADLLNGQAVPVYGPAG
EAIEHLTHRLKNGDHVSIAAPALELSVLDVPGHTSGHIAYFQAADPRGTPHVFCGDTLFACGCGRLFEGTPKQMLASLDA
LAALPGATEVHCAHEYTLSNIRFALACEPANAELQAWRDKAGELRARGVPTLPTTIAHERAVNPFLRAGNPAVQASLREQ
LHEKVPDRLTAFTLMREWKNRFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=261, Percent_Identity=38.3141762452107, Blast_Score=164, Evalue=5e-41,
Organism=Homo sapiens, GI94538322, Length=261, Percent_Identity=38.3141762452107, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI116642887, Length=241, Percent_Identity=39.0041493775934, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI21703352, Length=241, Percent_Identity=39.0041493775934, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI14150041, Length=232, Percent_Identity=37.5, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI46361987, Length=194, Percent_Identity=37.6288659793814, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1786406, Length=256, Percent_Identity=44.53125, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787158, Length=176, Percent_Identity=33.5227272727273, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=257, Percent_Identity=35.7976653696498, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6320478, Length=269, Percent_Identity=29.7397769516729, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324614, Length=243, Percent_Identity=27.5720164609054, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356335, Length=240, Percent_Identity=40.8333333333333, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24667711, Length=240, Percent_Identity=40.8333333333333, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24667703, Length=240, Percent_Identity=40.8333333333333, Blast_Score=165, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28517; Mature: 28517

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALEYVPVPAFDDNYIWVVSDGHSAVVVDPGEAAPVQAYLAKRGWRLSAILLTHHHQDH
CCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEEECCCHH
VGGVADLLNGQAVPVYGPAGEAIEHLTHRLKNGDHVSIAAPALELSVLDVPGHTSGHIAY
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCCCCEEE
FQAADPRGTPHVFCGDTLFACGCGRLFEGTPKQMLASLDALAALPGATEVHCAHEYTLSN
EEECCCCCCCEEEECCHHEECCCCHHCCCCHHHHHHHHHHHHHCCCCCEEEEEHHEECCC
IRFALACEPANAELQAWRDKAGELRARGVPTLPTTIAHERAVNPFLRAGNPAVQASLREQ
EEEEEEECCCCCHHHHHHHHHHCHHCCCCCCCCHHHHHHHHCCHHHHCCCHHHHHHHHHH
LHEKVPDRLTAFTLMREWKNRFR
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNALEYVPVPAFDDNYIWVVSDGHSAVVVDPGEAAPVQAYLAKRGWRLSAILLTHHHQDH
CCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEEECCCHH
VGGVADLLNGQAVPVYGPAGEAIEHLTHRLKNGDHVSIAAPALELSVLDVPGHTSGHIAY
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCCCCEEE
FQAADPRGTPHVFCGDTLFACGCGRLFEGTPKQMLASLDALAALPGATEVHCAHEYTLSN
EEECCCCCCCEEEECCHHEECCCCHHCCCCHHHHHHHHHHHHHCCCCCEEEEEHHEECCC
IRFALACEPANAELQAWRDKAGELRARGVPTLPTTIAHERAVNPFLRAGNPAVQASLREQ
EEEEEEECCCCCHHHHHHHHHHCHHCCCCCCCCHHHHHHHHCCHHHHCCCHHHHHHHHHH
LHEKVPDRLTAFTLMREWKNRFR
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA