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The map label for this gene is rlmE [H]

Identifier: 91784538

GI number: 91784538

Start: 3526720

End: 3527382

Strand: Reverse

Name: rlmE [H]

Synonym: Bxe_A1261

Alternate gene names: 91784538

Gene position: 3527382-3526720 (Counterclockwise)

Preceding gene: 91784540

Following gene: 91784537

Centisome position: 72.05

GC content: 58.22

Gene sequence:

>663_bases
ATGGCAAAAAACAAGTTCAATACCGCGTGGCTGCATGACCACATCAACGATCCGTACGTGAAAATGGCGCAGCGGGAAGG
CTATCGCGCCCGTGCAGCCTACAAGCTCAAGGAAATCGACGAACAGGACAAGCTGATCCGCGCGGGGCAGGTCATCGTCG
ACCTCGGCTCGACGCCGGGCAGCTGGAGCCAGTACGCGCGCAACAAGCTTGCCAAAGGCGCCCAGCGCGACGCCGAGCGC
GAAGGCGGGATCGACGGCACGATCATCGCGCTGGACCTGCTGCCAATGGAGCCGATCGCCGACGTCCACTTCATTCAGGG
CGACTTCCGCGAAGACTCGGTTATCGCGCAACTGGAAGAATTGGTCGGAGAACGCCAGGTTGACCTTGTAATTTCGGATA
TGGCTCCCAACCTGTCGGGAGTGGCGGTGGCGGATGCCGCGCGGATCGAGCATCTTTGTGATCTCGCACTGGAATTCTCG
CAAAACCACCTGAAACCGGATGGTGCCCTTTTAGTCAAATGCTTTCACGGCAGCGGTTACAGCCAGATTGTCGAAAAGTT
CAAGCGTCAGTTCAGGGTGGTGGCGGCCCGCAAGCCGAAAGCGTCGCGGGATAAATCGTCAGAAACGTTTATTTTGGGTA
AGCATCTCAAGCGGCCCGCATAG

Upstream 100 bases:

>100_bases
ACAGAATCGCGGATGGCCGCCAGTTTTGGCGGTGGCCGCGCGAATTAACCGCGTATTATCCGCTAAAGCGTGACGTCACG
CAGCAAGAGTTCAACGTTTA

Downstream 100 bases:

>100_bases
GCGCGCTGCAGGAAGGTTCCGCATCTGCCGTTAATGCGACCACGGCCTTGGAAAAGGGTGCTGCAATGGCACGTTGTCCT
ATTTCTGTGGTAGTGAAACC

Product: putative ribosomal RNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKNKFNTAWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRAGQVIVDLGSTPGSWSQYARNKLAKGAQRDAER
EGGIDGTIIALDLLPMEPIADVHFIQGDFREDSVIAQLEELVGERQVDLVISDMAPNLSGVAVADAARIEHLCDLALEFS
QNHLKPDGALLVKCFHGSGYSQIVEKFKRQFRVVAARKPKASRDKSSETFILGKHLKRPA

Sequences:

>Translated_220_residues
MAKNKFNTAWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRAGQVIVDLGSTPGSWSQYARNKLAKGAQRDAER
EGGIDGTIIALDLLPMEPIADVHFIQGDFREDSVIAQLEELVGERQVDLVISDMAPNLSGVAVADAARIEHLCDLALEFS
QNHLKPDGALLVKCFHGSGYSQIVEKFKRQFRVVAARKPKASRDKSSETFILGKHLKRPA
>Mature_219_residues
AKNKFNTAWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRAGQVIVDLGSTPGSWSQYARNKLAKGAQRDAERE
GGIDGTIIALDLLPMEPIADVHFIQGDFREDSVIAQLEELVGERQVDLVISDMAPNLSGVAVADAARIEHLCDLALEFSQ
NHLKPDGALLVKCFHGSGYSQIVEKFKRQFRVVAARKPKASRDKSSETFILGKHLKRPA

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=204, Percent_Identity=34.3137254901961, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI194097365, Length=203, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI29029591, Length=203, Percent_Identity=31.0344827586207, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI29029589, Length=203, Percent_Identity=31.0344827586207, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI7110661, Length=203, Percent_Identity=31.0344827586207, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1789569, Length=206, Percent_Identity=43.2038834951456, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17553474, Length=226, Percent_Identity=30.5309734513274, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17554650, Length=197, Percent_Identity=32.994923857868, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71987550, Length=203, Percent_Identity=29.5566502463054, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71987561, Length=203, Percent_Identity=29.5566502463054, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=203, Percent_Identity=29.5566502463054, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=204, Percent_Identity=31.3725490196078, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=207, Percent_Identity=31.8840579710145, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321302, Length=102, Percent_Identity=39.2156862745098, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356387, Length=218, Percent_Identity=32.5688073394495, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647580, Length=204, Percent_Identity=32.843137254902, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648639, Length=203, Percent_Identity=32.512315270936, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI18859957, Length=196, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24549; Mature: 24418

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKFNTAWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRAGQVIVDLGSTPG
CCCCCCCCHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCEEEEECCCCCC
SWSQYARNKLAKGAQRDAEREGGIDGTIIALDLLPMEPIADVHFIQGDFREDSVIAQLEE
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHH
LVGERQVDLVISDMAPNLSGVAVADAARIEHLCDLALEFSQNHLKPDGALLVKCFHGSGY
HHCCHHEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCH
SQIVEKFKRQFRVVAARKPKASRDKSSETFILGKHLKRPA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
AKNKFNTAWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRAGQVIVDLGSTPG
CCCCCCCHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCEEEEECCCCCC
SWSQYARNKLAKGAQRDAEREGGIDGTIIALDLLPMEPIADVHFIQGDFREDSVIAQLEE
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHH
LVGERQVDLVISDMAPNLSGVAVADAARIEHLCDLALEFSQNHLKPDGALLVKCFHGSGY
HHCCHHEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCH
SQIVEKFKRQFRVVAARKPKASRDKSSETFILGKHLKRPA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA