BacMap

Definition	Burkholderia xenovorans LB400 chromosome 1, complete sequence.
Accession	NC_007951
Length	4,895,836

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The map label for this gene is braG [H]

Identifier: 91784478

GI number: 91784478

Start: 3466990

End: 3467703

Strand: Reverse

Name: braG [H]

Synonym: Bxe_A1321

Alternate gene names: 91784478

Gene position: 3467703-3466990 (Counterclockwise)

Preceding gene: 91784479

Following gene: 91784477

Centisome position: 70.83

GC content: 69.19

Gene sequence:

>714_bases
ATGAGCGCCCTGCTCGACATTCGCGGGTTGAACGCGTGGTACGGCGCGAGCCAGGCGCTGCACGGCGTCACGCTGGGCAT
CGAGGCGGGCGAGGTGCTGGCGCTGGTCGGCCGCAACGGCTCGGGCCGCTCCACGCTTGCGCGCGCGATCATGGGCCTCG
TGCGCAGCGAAGGTGAACTGCGTTTCGCGGGCCGCGCGCTCGGCGGTCTGCGCACCTTCGAGATCGCGCGTCTCGGGATC
GGCTATGTGCCGGAACATCGCGATGTGTTTCCCGCGCTGAGCGTCCACGAGAATCTGCAACTCGGTGTCGCGCCTGGTGA
TCGCGGCCGCGCGCCGCGTTTCACCTTCGACGACGCCTACACGCTGTTCCCCGTGCTGCGCGAGCGCAGGCGCACGCGTG
CCGGCGCGCTCTCGGGCGGCGAGCAGCAGATGCTGACGCTCGCGCGGGCGCTGCTGGGCGACCCCGATCTGCTTGTCATC
GACGAACCCGGCGAGGGGCTCGCGAGTCTCGTGATGGAGCAGGTCTCCCGATGCTTGCGGACGCTGCGCGATCGCGGCGT
GGCGATGCTGCTGATCGAACAGCGCCTTGTGATCGCACAGGATATTGCCAGCCGCGTCGCCGTGATGGGGCATGGCGAAG
TCGTGTTCGACGGCGCGCTCGCGTCGTTCCTGCAGCGGACCGATGTGATGCGGGAATGGCTCGGTGTCGGTTGA

Upstream 100 bases:

>100_bases
CGGTTCTCGTGCAGGGGCGCATCATCGCTAGCGGCACGCCGGCGGCGATCCGCGCGGACGCGGCGGTGCGCGCCGCGTAT
CTCGGCGAAGGGTTCGGAAC

Downstream 100 bases:

>100_bases
GCGCCACACGTATTACCAGCGTTTTTATTGCAGTGCCGCATGGCGATGGACGATCGTTTTGCTGTCCTGCTGAATGCATC
GTGCGCGCGGAGCTTGAATG

Product: branched chain amino acid ABC transporter ATPase

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSALLDIRGLNAWYGASQALHGVTLGIEAGEVLALVGRNGSGRSTLARAIMGLVRSEGELRFAGRALGGLRTFEIARLGI
GYVPEHRDVFPALSVHENLQLGVAPGDRGRAPRFTFDDAYTLFPVLRERRRTRAGALSGGEQQMLTLARALLGDPDLLVI
DEPGEGLASLVMEQVSRCLRTLRDRGVAMLLIEQRLVIAQDIASRVAVMGHGEVVFDGALASFLQRTDVMREWLGVG

Sequences:

>Translated_237_residues
MSALLDIRGLNAWYGASQALHGVTLGIEAGEVLALVGRNGSGRSTLARAIMGLVRSEGELRFAGRALGGLRTFEIARLGI
GYVPEHRDVFPALSVHENLQLGVAPGDRGRAPRFTFDDAYTLFPVLRERRRTRAGALSGGEQQMLTLARALLGDPDLLVI
DEPGEGLASLVMEQVSRCLRTLRDRGVAMLLIEQRLVIAQDIASRVAVMGHGEVVFDGALASFLQRTDVMREWLGVG
>Mature_236_residues
SALLDIRGLNAWYGASQALHGVTLGIEAGEVLALVGRNGSGRSTLARAIMGLVRSEGELRFAGRALGGLRTFEIARLGIG
YVPEHRDVFPALSVHENLQLGVAPGDRGRAPRFTFDDAYTLFPVLRERRRTRAGALSGGEQQMLTLARALLGDPDLLVID
EPGEGLASLVMEQVSRCLRTLRDRGVAMLLIEQRLVIAQDIASRVAVMGHGEVVFDGALASFLQRTDVMREWLGVG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=225, Percent_Identity=31.1111111111111, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI27436953, Length=225, Percent_Identity=29.7777777777778, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI6005701, Length=209, Percent_Identity=26.7942583732057, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI27477115, Length=209, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI27262624, Length=193, Percent_Identity=23.3160621761658, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27262626, Length=193, Percent_Identity=23.3160621761658, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI255708477, Length=219, Percent_Identity=29.2237442922374, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI148612844, Length=219, Percent_Identity=29.2237442922374, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI47078218, Length=214, Percent_Identity=24.2990654205607, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI45446740, Length=214, Percent_Identity=24.2990654205607, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI11967969, Length=220, Percent_Identity=26.8181818181818, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI42490749, Length=207, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87082268, Length=236, Percent_Identity=37.7118644067797, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789672, Length=228, Percent_Identity=30.2631578947368, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787089, Length=245, Percent_Identity=30.6122448979592, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790190, Length=239, Percent_Identity=28.8702928870293, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786872, Length=240, Percent_Identity=30, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789593, Length=236, Percent_Identity=29.2372881355932, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787029, Length=221, Percent_Identity=29.4117647058824, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1789991, Length=228, Percent_Identity=29.3859649122807, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787792, Length=231, Percent_Identity=29.8701298701299, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI48995001, Length=222, Percent_Identity=28.3783783783784, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789864, Length=252, Percent_Identity=28.1746031746032, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788450, Length=241, Percent_Identity=29.8755186721992, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1786319, Length=230, Percent_Identity=30.4347826086957, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790544, Length=243, Percent_Identity=30.0411522633745, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1788761, Length=216, Percent_Identity=29.1666666666667, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788225, Length=238, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1788506, Length=258, Percent_Identity=31.7829457364341, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788472, Length=228, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788897, Length=216, Percent_Identity=31.4814814814815, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788165, Length=232, Percent_Identity=31.0344827586207, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787500, Length=231, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787105, Length=223, Percent_Identity=29.5964125560538, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1790162, Length=224, Percent_Identity=25.4464285714286, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081791, Length=219, Percent_Identity=27.8538812785388, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786398, Length=205, Percent_Identity=29.2682926829268, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994883, Length=229, Percent_Identity=28.82096069869, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789873, Length=217, Percent_Identity=28.110599078341, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787712, Length=216, Percent_Identity=25.9259259259259, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790525, Length=235, Percent_Identity=24.2553191489362, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI87081834, Length=197, Percent_Identity=28.4263959390863, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI87082267, Length=208, Percent_Identity=29.8076923076923, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786703, Length=187, Percent_Identity=33.6898395721925, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693107, Length=219, Percent_Identity=31.0502283105023, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788210, Length=199, Percent_Identity=26.1306532663317, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788644, Length=240, Percent_Identity=27.5, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1786563, Length=211, Percent_Identity=27.9620853080569, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI48994997, Length=227, Percent_Identity=29.5154185022026, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994943, Length=223, Percent_Identity=28.2511210762332, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787112, Length=227, Percent_Identity=30.8370044052863, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787370, Length=239, Percent_Identity=26.7782426778243, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081782, Length=202, Percent_Identity=31.1881188118812, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786253, Length=213, Percent_Identity=26.7605633802817, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790467, Length=215, Percent_Identity=26.046511627907, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787164, Length=212, Percent_Identity=28.7735849056604, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789032, Length=228, Percent_Identity=28.0701754385965, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87081709, Length=215, Percent_Identity=25.1162790697674, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786345, Length=213, Percent_Identity=26.7605633802817, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI1790534, Length=203, Percent_Identity=28.5714285714286, Blast_Score=60, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=224, Percent_Identity=29.9107142857143, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533608, Length=207, Percent_Identity=25.1207729468599, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983523, Length=207, Percent_Identity=29.4685990338164, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71983516, Length=207, Percent_Identity=29.4685990338164, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=207, Percent_Identity=29.4685990338164, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=205, Percent_Identity=27.3170731707317, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=205, Percent_Identity=27.3170731707317, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71984940, Length=202, Percent_Identity=29.2079207920792, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI193211017, Length=205, Percent_Identity=27.3170731707317, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17565586, Length=208, Percent_Identity=25.9615384615385, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI212646699, Length=221, Percent_Identity=27.1493212669683, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539902, Length=203, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323217, Length=216, Percent_Identity=30.5555555555556, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24666092, Length=233, Percent_Identity=27.4678111587983, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648314, Length=222, Percent_Identity=23.4234234234234, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574533, Length=227, Percent_Identity=27.7533039647577, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24643674, Length=227, Percent_Identity=25.9911894273128, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574744, Length=215, Percent_Identity=26.5116279069767, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221512771, Length=210, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24649703, Length=216, Percent_Identity=29.1666666666667, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI85726357, Length=215, Percent_Identity=28.8372093023256, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221500365, Length=208, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116007184, Length=208, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24662800, Length=214, Percent_Identity=26.1682242990654, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641565, Length=208, Percent_Identity=27.8846153846154, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25599; Mature: 25468

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALLDIRGLNAWYGASQALHGVTLGIEAGEVLALVGRNGSGRSTLARAIMGLVRSEGEL
CCCCEECCCCCHHHHHHHHHCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCE
RFAGRALGGLRTFEIARLGIGYVPEHRDVFPALSVHENLQLGVAPGDRGRAPRFTFDDAY
EEHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCEECCCHH
TLFPVLRERRRTRAGALSGGEQQMLTLARALLGDPDLLVIDEPGEGLASLVMEQVSRCLR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHH
TLRDRGVAMLLIEQRLVIAQDIASRVAVMGHGEVVFDGALASFLQRTDVMREWLGVG
HHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SALLDIRGLNAWYGASQALHGVTLGIEAGEVLALVGRNGSGRSTLARAIMGLVRSEGEL
CCCEECCCCCHHHHHHHHHCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCE
RFAGRALGGLRTFEIARLGIGYVPEHRDVFPALSVHENLQLGVAPGDRGRAPRFTFDDAY
EEHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCEECCCHH
TLFPVLRERRRTRAGALSGGEQQMLTLARALLGDPDLLVIDEPGEGLASLVMEQVSRCLR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHH
TLRDRGVAMLLIEQRLVIAQDIASRVAVMGHGEVVFDGALASFLQRTDVMREWLGVG
HHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]