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The map label for this gene is boxB [H]

Identifier: 91784377

GI number: 91784377

Start: 3344989

End: 3346416

Strand: Reverse

Name: boxB [H]

Synonym: Bxe_A1423

Alternate gene names: 91784377

Gene position: 3346416-3344989 (Counterclockwise)

Preceding gene: 91784378

Following gene: 91784376

Centisome position: 68.35

GC content: 61.2

Gene sequence:

>1428_bases
ATGTCCACGATCAACTACAGCGAAAAGATTCCGAACAACGTCAACCTCGCCGACGACCGCACGCTGCAACGCGCGCTCGA
ACAATGGCAGCCGAATTTTCTGGCCTGGTGGGGCGACATGGGCCCGGAAGGCTCGCACGGCTACGATGTGTATCTGCGCA
CTGCAGTGAGCGTGGACGCCGGCGGCTGGGCGCATTTCGACCATGTGAAGATGCCGGACTACCGCTGGGGCATTTTCCTG
ACGCCCGGCGAGCAGGACCGCAAGATTCACTTCGGCGAACACAAGGGCGAAGCGGCCTGGCAGGACGTGCCCGGCGAGCA
TCGCGCCAATTTGCGGCGCATTATCGTGACGCAAGGCGATACGGAACCGGCGTCGGTCGAGCAGCAACGCCACCTTGGTC
TGACGGCGCCCTCCATTTACGACTTGCGCAACCTGTTCCAGGTGAATGTGGAAGAGGGGCGCCACCTGTGGGCGATGGTG
TATCTGTTGCATCGCTATTTCGGCCGCGACGGCCGTGAGGAAGCCGAAGCATTGCTCGGCCGCCGTTCAGGCGATGACGA
CAACCCGCGCATTCTGGGCGCGTTCAATGAGAAAACGCCGGACTGGCTCGCGTTCTTCATGTTCACGTACTTTACCGATC
GCGACGGCAAATTCCAGTTGTCGGCGCTGGCCGAGTCGGGCTTCGATCCGCTCGCGCGCACCACGAAGTTCATGCTCACT
GAAGAAGCGCACCACATGTTCGTCGGCGAGTCGGGTGTTTCGCGGGTCATCCAGCGCACCGCCAAGGTGATGAACGAACT
GGGCACGGACGACGTCACGAAGATTCGCGCGGCCGGCGTGATCGATCTGCCGACCATTCAGCGTTATCTGAACTTCCACT
ACTCGGTCACTATCGATCTGTTCGGCGCGGATCATTCTTCCAACGCGGCCACGTTCTATAGCTCGGGCCTGAAGGGGCGC
TATGAAGAAAACAGGCGCGAGGACGATCATCAACTGAACGGCCAGAGTTACAAGCTGCTCGACGTGCAGGACGGCAAGCT
GGTCGAGCGCGAAGTGCCGATGCTCAATGCGATGAACGAAGTGCTGCGCGACGATTACATCAAGGATTCGGTGGCGGGCG
TGGCGCGCTGGAACAAGGTGCTCGAAAAGGCCGGCATCGATTTCCGCATGACGGTGCCGCATAAGGCGTTCAATCGTCAG
ATCGGCACGTTCGCCGGCACGCGCGTATCGCCCGACGGTCGCGTGGTCAGCGAAACCGAATGGGCCGCCAACGAAGCGAA
ATGGCTCGCCACGCCGGAAGACCGCGCGTACGTCGCGTCGTTGATGGGACGCGTCACGGAAGCCGGCAAGTTCGCCAACT
GGATCGCGCCGCCGGCAATGGGTGTGAACCGTCAGCCGGTCGATTTCGAATACGTGCGCTTCAACTGA

Upstream 100 bases:

>100_bases
ACGCCATCGACGCCATCGACGCAGCGAGCGCGAGCCGGAGCGCCACTTGCCCGACACGCGCCTCTCATCGTCACTGCAGT
TTCCGAACCAGGAACCGACT

Downstream 100 bases:

>100_bases
ACGCGCGGGCGGCGCGCGGCACGCTTGCGCTGCATATCCAGGTGGAGGAAGTCATGAACGGCCCAGTATCGATCGAAGTT
CTCAGGCAGCATCTGATCGA

Product: benzoyl-CoA oxygenase, component B

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit B; Benzoyl-CoA dioxygenase oxygenase component [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSTINYSEKIPNNVNLADDRTLQRALEQWQPNFLAWWGDMGPEGSHGYDVYLRTAVSVDAGGWAHFDHVKMPDYRWGIFL
TPGEQDRKIHFGEHKGEAAWQDVPGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSIYDLRNLFQVNVEEGRHLWAMV
YLLHRYFGRDGREEAEALLGRRSGDDDNPRILGAFNEKTPDWLAFFMFTYFTDRDGKFQLSALAESGFDPLARTTKFMLT
EEAHHMFVGESGVSRVIQRTAKVMNELGTDDVTKIRAAGVIDLPTIQRYLNFHYSVTIDLFGADHSSNAATFYSSGLKGR
YEENRREDDHQLNGQSYKLLDVQDGKLVEREVPMLNAMNEVLRDDYIKDSVAGVARWNKVLEKAGIDFRMTVPHKAFNRQ
IGTFAGTRVSPDGRVVSETEWAANEAKWLATPEDRAYVASLMGRVTEAGKFANWIAPPAMGVNRQPVDFEYVRFN

Sequences:

>Translated_475_residues
MSTINYSEKIPNNVNLADDRTLQRALEQWQPNFLAWWGDMGPEGSHGYDVYLRTAVSVDAGGWAHFDHVKMPDYRWGIFL
TPGEQDRKIHFGEHKGEAAWQDVPGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSIYDLRNLFQVNVEEGRHLWAMV
YLLHRYFGRDGREEAEALLGRRSGDDDNPRILGAFNEKTPDWLAFFMFTYFTDRDGKFQLSALAESGFDPLARTTKFMLT
EEAHHMFVGESGVSRVIQRTAKVMNELGTDDVTKIRAAGVIDLPTIQRYLNFHYSVTIDLFGADHSSNAATFYSSGLKGR
YEENRREDDHQLNGQSYKLLDVQDGKLVEREVPMLNAMNEVLRDDYIKDSVAGVARWNKVLEKAGIDFRMTVPHKAFNRQ
IGTFAGTRVSPDGRVVSETEWAANEAKWLATPEDRAYVASLMGRVTEAGKFANWIAPPAMGVNRQPVDFEYVRFN
>Mature_474_residues
STINYSEKIPNNVNLADDRTLQRALEQWQPNFLAWWGDMGPEGSHGYDVYLRTAVSVDAGGWAHFDHVKMPDYRWGIFLT
PGEQDRKIHFGEHKGEAAWQDVPGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSIYDLRNLFQVNVEEGRHLWAMVY
LLHRYFGRDGREEAEALLGRRSGDDDNPRILGAFNEKTPDWLAFFMFTYFTDRDGKFQLSALAESGFDPLARTTKFMLTE
EAHHMFVGESGVSRVIQRTAKVMNELGTDDVTKIRAAGVIDLPTIQRYLNFHYSVTIDLFGADHSSNAATFYSSGLKGRY
EENRREDDHQLNGQSYKLLDVQDGKLVEREVPMLNAMNEVLRDDYIKDSVAGVARWNKVLEKAGIDFRMTVPHKAFNRQI
GTFAGTRVSPDGRVVSETEWAANEAKWLATPEDRAYVASLMGRVTEAGKFANWIAPPAMGVNRQPVDFEYVRFN

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxB acts as the benzoyl-CoA oxygenase, after being reduced by the reductase component BoxA. BoxAB does not

COG id: COG3396

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the benzoyl-CoA oxygenase component B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017635
- InterPro:   IPR012347
- InterPro:   IPR009078
- InterPro:   IPR007814 [H]

Pfam domain/function: PF05138 PaaA_PaaC [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 54059; Mature: 53928

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTINYSEKIPNNVNLADDRTLQRALEQWQPNFLAWWGDMGPEGSHGYDVYLRTAVSVDA
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEEEEECC
GGWAHFDHVKMPDYRWGIFLTPGEQDRKIHFGEHKGEAAWQDVPGEHRANLRRIIVTQGD
CCCEECCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCHHCCCCCHHHCCEEEEEEECCC
TEPASVEQQRHLGLTAPSIYDLRNLFQVNVEEGRHLWAMVYLLHRYFGRDGREEAEALLG
CCCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHC
RRSGDDDNPRILGAFNEKTPDWLAFFMFTYFTDRDGKFQLSALAESGFDPLARTTKFMLT
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHEE
EEAHHMFVGESGVSRVIQRTAKVMNELGTDDVTKIRAAGVIDLPTIQRYLNFHYSVTIDL
CCCHHEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEHHHHHHHHCCEEEEEEEE
FGADHSSNAATFYSSGLKGRYEENRREDDHQLNGQSYKLLDVQDGKLVEREVPMLNAMNE
EECCCCCCCEEEHHCCCCCCCHHHCCCCHHHCCCCCEEEEECCCCCCHHHHCHHHHHHHH
VLRDDYIKDSVAGVARWNKVLEKAGIDFRMTVPHKAFNRQIGTFAGTRVSPDGRVVSETE
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCCCCEECCCCCEEECCH
WAANEAKWLATPEDRAYVASLMGRVTEAGKFANWIAPPAMGVNRQPVDFEYVRFN
HCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
STINYSEKIPNNVNLADDRTLQRALEQWQPNFLAWWGDMGPEGSHGYDVYLRTAVSVDA
CCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEEEEECC
GGWAHFDHVKMPDYRWGIFLTPGEQDRKIHFGEHKGEAAWQDVPGEHRANLRRIIVTQGD
CCCEECCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCHHCCCCCHHHCCEEEEEEECCC
TEPASVEQQRHLGLTAPSIYDLRNLFQVNVEEGRHLWAMVYLLHRYFGRDGREEAEALLG
CCCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHC
RRSGDDDNPRILGAFNEKTPDWLAFFMFTYFTDRDGKFQLSALAESGFDPLARTTKFMLT
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHEE
EEAHHMFVGESGVSRVIQRTAKVMNELGTDDVTKIRAAGVIDLPTIQRYLNFHYSVTIDL
CCCHHEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEHHHHHHHHCCEEEEEEEE
FGADHSSNAATFYSSGLKGRYEENRREDDHQLNGQSYKLLDVQDGKLVEREVPMLNAMNE
EECCCCCCCEEEHHCCCCCCCHHHCCCCHHHCCCCCEEEEECCCCCCHHHHCHHHHHHHH
VLRDDYIKDSVAGVARWNKVLEKAGIDFRMTVPHKAFNRQIGTFAGTRVSPDGRVVSETE
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCCCCEECCCCCEEECCH
WAANEAKWLATPEDRAYVASLMGRVTEAGKFANWIAPPAMGVNRQPVDFEYVRFN
HCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]