Click here to switch to the map view.

The map label for this gene is boxA [H]

Identifier: 91784376

GI number: 91784376

Start: 3343694

End: 3344935

Strand: Reverse

Name: boxA [H]

Synonym: Bxe_A1424

Alternate gene names: 91784376

Gene position: 3344935-3343694 (Counterclockwise)

Preceding gene: 91784377

Following gene: 91784373

Centisome position: 68.32

GC content: 61.19

Gene sequence:

>1242_bases
ATGAACGGCCCAGTATCGATCGAAGTTCTCAGGCAGCATCTGATCGATCCGGAAATCTGCATTCGCTGCAATACGTGCGA
AGAGACTTGCCCGGTCGATGCGATCACGCACGACGACAACAACTACGTGGTCAAGGCGGATATCTGCAACGGCTGCATGG
CGTGTGTGCCGCCGTGTCCGACCGGCGCGATCGACAACTGGCGCACGGTGCTGAAAGCCGACGCGTACCCGATCGACGAG
CAGTTCACGTGGGACGTGCTGCCGGAACAGAACACGATGGCCGTGGCCGCCGCGGACGAACTGTCGGGCGCGGGCGCGTC
GGGCGATACGCCAGCGGACGCGAGCGGCATCGAAGTGGATACCGTGCGGGGTTCGGTCGTGCCGCCGTGGTCGGCCGCTA
AGCCCTATGTGAATCTCTACACGCACAAGGCGCCGACTACCGCAACGGTGGTCGGCAATTACCGGTTGACCGATGGTTCG
ACCGACAGCGACATTCATCATATCGTGCTGGATTTCGGCGCTATGCCGTTTCCGGTGCTGGAAGGACAGTCGATCGGCAT
TTTGCCGCCTGGCTCGACTGCCGACGGCCGCGCGCATCACGCGCGCCAGTATTCGATCGCGAGCCCGCGCGACGGCGAGC
GGCCCGGTTATAACAACATCTCGCTGACGGTCAAACGAGTATCGCAGCAGCACGGCGATTCGACTGATGGCGTATGTTCG
AACTATCTGTGCGATCTGAAGAAGGGCGACGTGGTCAATGTGATCGGCCCGTTCGGCGGAACCTTCCTGATGCCGAATCA
TCCGAACTCGCATCTGCTGATGATCTGCACGGGTACGGGTTCAGCGCCGATGCGCGCGATGACCGAGTATCGTCGCCGGC
GCCGTCTGAAGGGCGCAACGGGCAAGCTGATGTTGTTCTTCGGCGCGCGGACCAAAGAAGAGTTGCCGTACTTCGGGCCC
CTTACGAATCTGCCGAAGGATTTCATCGATACCAATCTGGCGTTTTCGCGCACGCCGGGCCAACCAAAGCGCTATGTTCA
GGACGCCATGCGCGAGCGCGCGGTCGACGTGGCGCATATGCTCAGGGATGAGAATACGCATATCTACGTGTGCGGGTTGA
AGGGTATGGAAGACGGCGTGCTACAGGCGCTGAAGGAGATCGGCGAGCAGCATCAGCTGGATTGGGATGGGCTGTGGGCG
AGGCTCAAGCGGGAGGGGAGGCTGCATCTGGAGACGTATTGA

Upstream 100 bases:

>100_bases
GTGTGAACCGTCAGCCGGTCGATTTCGAATACGTGCGCTTCAACTGAACGCGCGGGCGGCGCGCGGCACGCTTGCGCTGC
ATATCCAGGTGGAGGAAGTC

Downstream 100 bases:

>100_bases
CACCGGAACTCACCGTGCCGCCTCACAATCGTCTTCACCGGCAGACGCGGTGAGATTGTCGTATTTAACAACCGTGCCGC
GCAGCGCGTCGAGCGGGCAC

Product: benzoyl-CoA oxygenase, component A

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit A; Benzoyl-CoA dioxygenase reductase component [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MNGPVSIEVLRQHLIDPEICIRCNTCEETCPVDAITHDDNNYVVKADICNGCMACVPPCPTGAIDNWRTVLKADAYPIDE
QFTWDVLPEQNTMAVAAADELSGAGASGDTPADASGIEVDTVRGSVVPPWSAAKPYVNLYTHKAPTTATVVGNYRLTDGS
TDSDIHHIVLDFGAMPFPVLEGQSIGILPPGSTADGRAHHARQYSIASPRDGERPGYNNISLTVKRVSQQHGDSTDGVCS
NYLCDLKKGDVVNVIGPFGGTFLMPNHPNSHLLMICTGTGSAPMRAMTEYRRRRRLKGATGKLMLFFGARTKEELPYFGP
LTNLPKDFIDTNLAFSRTPGQPKRYVQDAMRERAVDVAHMLRDENTHIYVCGLKGMEDGVLQALKEIGEQHQLDWDGLWA
RLKREGRLHLETY

Sequences:

>Translated_413_residues
MNGPVSIEVLRQHLIDPEICIRCNTCEETCPVDAITHDDNNYVVKADICNGCMACVPPCPTGAIDNWRTVLKADAYPIDE
QFTWDVLPEQNTMAVAAADELSGAGASGDTPADASGIEVDTVRGSVVPPWSAAKPYVNLYTHKAPTTATVVGNYRLTDGS
TDSDIHHIVLDFGAMPFPVLEGQSIGILPPGSTADGRAHHARQYSIASPRDGERPGYNNISLTVKRVSQQHGDSTDGVCS
NYLCDLKKGDVVNVIGPFGGTFLMPNHPNSHLLMICTGTGSAPMRAMTEYRRRRRLKGATGKLMLFFGARTKEELPYFGP
LTNLPKDFIDTNLAFSRTPGQPKRYVQDAMRERAVDVAHMLRDENTHIYVCGLKGMEDGVLQALKEIGEQHQLDWDGLWA
RLKREGRLHLETY
>Mature_413_residues
MNGPVSIEVLRQHLIDPEICIRCNTCEETCPVDAITHDDNNYVVKADICNGCMACVPPCPTGAIDNWRTVLKADAYPIDE
QFTWDVLPEQNTMAVAAADELSGAGASGDTPADASGIEVDTVRGSVVPPWSAAKPYVNLYTHKAPTTATVVGNYRLTDGS
TDSDIHHIVLDFGAMPFPVLEGQSIGILPPGSTADGRAHHARQYSIASPRDGERPGYNNISLTVKRVSQQHGDSTDGVCS
NYLCDLKKGDVVNVIGPFGGTFLMPNHPNSHLLMICTGTGSAPMRAMTEYRRRRRLKGATGKLMLFFGARTKEELPYFGP
LTNLPKDFIDTNLAFSRTPGQPKRYVQDAMRERAVDVAHMLRDENTHIYVCGLKGMEDGVLQALKEIGEQHQLDWDGLWA
RLKREGRLHLETY

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxA acts as a reductase that uses NADPH to reduce the oxygenase component BoxB. BoxAB does not act on NADH

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=228, Percent_Identity=31.140350877193, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI10835173, Length=226, Percent_Identity=29.646017699115, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI40254422, Length=201, Percent_Identity=32.8358208955224, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789123, Length=222, Percent_Identity=31.5315315315315, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321143, Length=224, Percent_Identity=29.9107142857143, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583543, Length=222, Percent_Identity=29.2792792792793, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78706876, Length=222, Percent_Identity=29.2792792792793, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78706872, Length=222, Percent_Identity=29.2792792792793, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24660907, Length=182, Percent_Identity=34.0659340659341, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24660903, Length=182, Percent_Identity=34.0659340659341, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017634
- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012146
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00037 Fer4; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 45308; Mature: 45308

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGPVSIEVLRQHLIDPEICIRCNTCEETCPVDAITHDDNNYVVKADICNGCMACVPPCP
CCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEEECCCCCEECCCCCC
TGAIDNWRTVLKADAYPIDEQFTWDVLPEQNTMAVAAADELSGAGASGDTPADASGIEVD
CCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEECHHHCCCCCCCCCCCCCCCEEEE
TVRGSVVPPWSAAKPYVNLYTHKAPTTATVVGNYRLTDGSTDSDIHHIVLDFGAMPFPVL
ECCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCEE
EGQSIGILPPGSTADGRAHHARQYSIASPRDGERPGYNNISLTVKRVSQQHGDSTDGVCS
CCCEEEEECCCCCCCCCHHHHHEECCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCHHH
NYLCDLKKGDVVNVIGPFGGTFLMPNHPNSHLLMICTGTGSAPMRAMTEYRRRRRLKGAT
HHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC
GKLMLFFGARTKEELPYFGPLTNLPKDFIDTNLAFSRTPGQPKRYVQDAMRERAVDVAHM
CCEEEEECCCCHHCCCCCCCHHCCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
LRDENTHIYVCGLKGMEDGVLQALKEIGEQHQLDWDGLWARLKREGRLHLETY
HCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MNGPVSIEVLRQHLIDPEICIRCNTCEETCPVDAITHDDNNYVVKADICNGCMACVPPCP
CCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEEECCCCCEECCCCCC
TGAIDNWRTVLKADAYPIDEQFTWDVLPEQNTMAVAAADELSGAGASGDTPADASGIEVD
CCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEECHHHCCCCCCCCCCCCCCCEEEE
TVRGSVVPPWSAAKPYVNLYTHKAPTTATVVGNYRLTDGSTDSDIHHIVLDFGAMPFPVL
ECCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCEE
EGQSIGILPPGSTADGRAHHARQYSIASPRDGERPGYNNISLTVKRVSQQHGDSTDGVCS
CCCEEEEECCCCCCCCCHHHHHEECCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCHHH
NYLCDLKKGDVVNVIGPFGGTFLMPNHPNSHLLMICTGTGSAPMRAMTEYRRRRRLKGAT
HHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC
GKLMLFFGARTKEELPYFGPLTNLPKDFIDTNLAFSRTPGQPKRYVQDAMRERAVDVAHM
CCEEEEECCCCHHCCCCCCCHHCCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
LRDENTHIYVCGLKGMEDGVLQALKEIGEQHQLDWDGLWARLKREGRLHLETY
HCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]