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The map label for this gene is yeiT [H]

Identifier: 91784372

GI number: 91784372

Start: 3339561

End: 3340910

Strand: Reverse

Name: yeiT [H]

Synonym: Bxe_A1428

Alternate gene names: 91784372

Gene position: 3340910-3339561 (Counterclockwise)

Preceding gene: 91784373

Following gene: 91784371

Centisome position: 68.24

GC content: 66.15

Gene sequence:

>1350_bases
ATGGCCATCAAGCAAACCGGCGACATTGCCGCTCAGCGCCTGTCGCCCGAGCAGCTCTCGTGCGAGTTCTCCGACATCGC
ACCGCTGCTCGACGCGAGCGCCGCGGCCGCGGCGGCGAGCCGCTGTCATTACTGTTACGACGCGCCGTGCGTGAACGCGT
GCCCCACGCAGATCGACATTCCCAGTTTCATCCGCAAGATCAGCAACGGCAATCTGAAGGGCGCGGCCGTGGACATTCTG
TCGGCCAACCCGATGGGCGGCATGTGCGCACGCGTCTGCCCCACGGAAATTCTTTGTGAAGGCGCTTGCGTGCGCAACCA
TCAGGATGCGAAACCAGTGGCGATCGGCGCGCTGCAACGTCATGCGACGGACTGGGCGATGGCGCGCGGCGAAGTGCTGT
TCAAGCGCGCGGCGGAAACCGGACGGCACGTCGCGGTGGTCGGCGCGGGTCCGGCGGGACTCGCCTGCGCGCATCGGCTT
GCGCTTGCCGGCCACACCGTGACGATCTACGACGCTCACGAAAAAGCCGGCGGCCTGAACGAATACGGCATTGCCGCCTA
CAAAACCGTCGATGATTTTGCGCAGCGTGAAGTGGCGTGGCTGTGCTCCGTTGGCGGTATCGAAATCAAACATGGCGTGG
CGCTTGGTCGCGATATCGAGCTCGACACGCTGCGCAAGCAGCACGACGCGGTGTTCCTCGCTATCGGCCTCGGCGGCGTG
CGGGCGCTCGCGATGGAAGGCGAAGACCTTGGCGGTGTGATGAACGCGGTGGACTTCATCGAACAGGTGCGCAGCGCGAG
CGACCTCGGCACGGTGCCGGTGGGCCGGCGCGTCGTCGTGATCGGCGGCGGCAATACGGCGGTGGATGCTGCCGTGCAAA
GCCGCAAGCTCGGCGCGGCGTCGGTGACGATGGTGTACCGGCGCGGCGTCGAAGCAATGAGCGCCACCTGGGCCGAGCGC
GACTTCGCTCAGACCAGTGGCGTGACGCTCATCACGCATGCCACGCCGATGCGGTTGATTGGCGACAACGGCGTGGTCAC
GGGTGTCGAATTCGCACGTGCGTCGAATGATGGCGGCGAGGAGCGCTTCGTAGTCGAAGCCGACATGGTGCTCAAGGCGA
TCGGTCAAACGCTGGTGCCGGTCGGCATCGGGCGCGAGTTGCTGACCTTGGACGGTAGCCGTATCGCCGTCGACGCGAAC
GGCCAGACCTCGCTGGCAAACGTATGGGCCGGCGGCGATTGCGCGGCCACTGGTGGCATCGATCTCACGGTGCAGGCGGT
TCAGGACGGCAAGGTCGCCGCTGCCGCTATCGATGCGCAGTTTGCCCGCACGGCGGCGAAAGCCGCCTGA

Upstream 100 bases:

>100_bases
ACCCGCTTTACCGTAGTGCCCATAAAACAAGCCGTCCCGGCTGCGCTCGAAGCGCGGCCGGCGGGAGCGGCTGCGTCCTC
ACATCGAAGAAGGAACGAGT

Downstream 100 bases:

>100_bases
AGCGAATCGAGCCAAATCACGTAACGAAAACAGACAAAAAACAGCAGTCCCCAAGGAGCCGAAAGCGAATCGAGCCAAAT
CACGTAACGAAAACAGACAA

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MAIKQTGDIAAQRLSPEQLSCEFSDIAPLLDASAAAAAASRCHYCYDAPCVNACPTQIDIPSFIRKISNGNLKGAAVDIL
SANPMGGMCARVCPTEILCEGACVRNHQDAKPVAIGALQRHATDWAMARGEVLFKRAAETGRHVAVVGAGPAGLACAHRL
ALAGHTVTIYDAHEKAGGLNEYGIAAYKTVDDFAQREVAWLCSVGGIEIKHGVALGRDIELDTLRKQHDAVFLAIGLGGV
RALAMEGEDLGGVMNAVDFIEQVRSASDLGTVPVGRRVVVIGGGNTAVDAAVQSRKLGAASVTMVYRRGVEAMSATWAER
DFAQTSGVTLITHATPMRLIGDNGVVTGVEFARASNDGGEERFVVEADMVLKAIGQTLVPVGIGRELLTLDGSRIAVDAN
GQTSLANVWAGGDCAATGGIDLTVQAVQDGKVAAAAIDAQFARTAAKAA

Sequences:

>Translated_449_residues
MAIKQTGDIAAQRLSPEQLSCEFSDIAPLLDASAAAAAASRCHYCYDAPCVNACPTQIDIPSFIRKISNGNLKGAAVDIL
SANPMGGMCARVCPTEILCEGACVRNHQDAKPVAIGALQRHATDWAMARGEVLFKRAAETGRHVAVVGAGPAGLACAHRL
ALAGHTVTIYDAHEKAGGLNEYGIAAYKTVDDFAQREVAWLCSVGGIEIKHGVALGRDIELDTLRKQHDAVFLAIGLGGV
RALAMEGEDLGGVMNAVDFIEQVRSASDLGTVPVGRRVVVIGGGNTAVDAAVQSRKLGAASVTMVYRRGVEAMSATWAER
DFAQTSGVTLITHATPMRLIGDNGVVTGVEFARASNDGGEERFVVEADMVLKAIGQTLVPVGIGRELLTLDGSRIAVDAN
GQTSLANVWAGGDCAATGGIDLTVQAVQDGKVAAAAIDAQFARTAAKAA
>Mature_448_residues
AIKQTGDIAAQRLSPEQLSCEFSDIAPLLDASAAAAAASRCHYCYDAPCVNACPTQIDIPSFIRKISNGNLKGAAVDILS
ANPMGGMCARVCPTEILCEGACVRNHQDAKPVAIGALQRHATDWAMARGEVLFKRAAETGRHVAVVGAGPAGLACAHRLA
LAGHTVTIYDAHEKAGGLNEYGIAAYKTVDDFAQREVAWLCSVGGIEIKHGVALGRDIELDTLRKQHDAVFLAIGLGGVR
ALAMEGEDLGGVMNAVDFIEQVRSASDLGTVPVGRRVVVIGGGNTAVDAAVQSRKLGAASVTMVYRRGVEAMSATWAERD
FAQTSGVTLITHATPMRLIGDNGVVTGVEFARASNDGGEERFVVEADMVLKAIGQTLVPVGIGRELLTLDGSRIAVDANG
QTSLANVWAGGDCAATGGIDLTVQAVQDGKVAAAAIDAQFARTAAKAA

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=455, Percent_Identity=32.967032967033, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI237757300, Length=109, Percent_Identity=45.8715596330275, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1788468, Length=417, Percent_Identity=34.2925659472422, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1789606, Length=464, Percent_Identity=32.5431034482759, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI87082180, Length=460, Percent_Identity=33.0434782608696, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1788811, Length=462, Percent_Identity=33.1168831168831, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789243, Length=381, Percent_Identity=27.5590551181102, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71984108, Length=449, Percent_Identity=33.8530066815145, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17570289, Length=450, Percent_Identity=27.3333333333333, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320030, Length=459, Percent_Identity=30.718954248366, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24640763, Length=453, Percent_Identity=33.9955849889625, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI18858217, Length=453, Percent_Identity=33.9955849889625, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24665547, Length=445, Percent_Identity=29.6629213483146, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24665543, Length=445, Percent_Identity=29.6629213483146, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI28574881, Length=445, Percent_Identity=29.6629213483146, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24665539, Length=445, Percent_Identity=29.6629213483146, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 46512; Mature: 46381

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKQTGDIAAQRLSPEQLSCEFSDIAPLLDASAAAAAASRCHYCYDAPCVNACPTQIDI
CCCCCCCHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCH
PSFIRKISNGNLKGAAVDILSANPMGGMCARVCPTEILCEGACVRNHQDAKPVAIGALQR
HHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHCCHHHHHCCCHHCCCCCCCCHHHHHHHH
HATDWAMARGEVLFKRAAETGRHVAVVGAGPAGLACAHRLALAGHTVTIYDAHEKAGGLN
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECHHHCCCCC
EYGIAAYKTVDDFAQREVAWLCSVGGIEIKHGVALGRDIELDTLRKQHDAVFLAIGLGGV
CCCEEEEHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCHHHHHHCCCEEEEEEECCCE
RALAMEGEDLGGVMNAVDFIEQVRSASDLGTVPVGRRVVVIGGGNTAVDAAVQSRKLGAA
EEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCHH
SVTMVYRRGVEAMSATWAERDFAQTSGVTLITHATPMRLIGDNGVVTGVEFARASNDGGE
HHHHHHHHCHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCCEECEEHEECCCCCCC
ERFVVEADMVLKAIGQTLVPVGIGRELLTLDGSRIAVDANGQTSLANVWAGGDCAATGGI
CEEEEEHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCEEEEECCCCEEECCCC
DLTVQAVQDGKVAAAAIDAQFARTAAKAA
EEEEEEECCCCEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIKQTGDIAAQRLSPEQLSCEFSDIAPLLDASAAAAAASRCHYCYDAPCVNACPTQIDI
CCCCCCHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCH
PSFIRKISNGNLKGAAVDILSANPMGGMCARVCPTEILCEGACVRNHQDAKPVAIGALQR
HHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHCCHHHHHCCCHHCCCCCCCCHHHHHHHH
HATDWAMARGEVLFKRAAETGRHVAVVGAGPAGLACAHRLALAGHTVTIYDAHEKAGGLN
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECHHHCCCCC
EYGIAAYKTVDDFAQREVAWLCSVGGIEIKHGVALGRDIELDTLRKQHDAVFLAIGLGGV
CCCEEEEHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCHHHHHHCCCEEEEEEECCCE
RALAMEGEDLGGVMNAVDFIEQVRSASDLGTVPVGRRVVVIGGGNTAVDAAVQSRKLGAA
EEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCHH
SVTMVYRRGVEAMSATWAERDFAQTSGVTLITHATPMRLIGDNGVVTGVEFARASNDGGE
HHHHHHHHCHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCCEECEEHEECCCCCCC
ERFVVEADMVLKAIGQTLVPVGIGRELLTLDGSRIAVDANGQTSLANVWAGGDCAATGGI
CEEEEEHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCEEEEECCCCEEECCCC
DLTVQAVQDGKVAAAAIDAQFARTAAKAA
EEEEEEECCCCEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]