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The map label for this gene is ptrB [C]

Identifier: 91784275

GI number: 91784275

Start: 3229603

End: 3231687

Strand: Reverse

Name: ptrB [C]

Synonym: Bxe_A1525

Alternate gene names: 91784275

Gene position: 3231687-3229603 (Counterclockwise)

Preceding gene: 91784276

Following gene: 91784273

Centisome position: 66.01

GC content: 65.76

Gene sequence:

>2085_bases
ATGTCGACGCCGTTCTGCTGGCCGCTTTCCCCCGATACCTTCATCGCGCTCGAGGCGCTCGACGATCCACTGGTGCTCGA
ATGGGTGGAACGGCAGAACGTGCGCACGCTTGCGGCATGGGCGAGCGGCGCGCGCTTCGACACGCTCAAGGACCGGCTCG
CTTCCGTCTACTTGCCGCGCGACCGGCCGGTCGTTCCCACGCGCTGGCGGGAGTGGGCGCACGACATCTGGCAGGACGGG
CACAATCCCAAAGGTATCTGGCGCCGCGCCTCGTGGGCCAACTGGCGTGCCGGTCAGGCCGTGTGGCAGACATTGCTCGA
TTTCGACGCGCTCGGCGAAGACGAGCAGATGCCGTGGGTGTGCGTCGAACGGCAGATCCTTTATCCAGATGGCGACCGGG
CGCTGATTTCGCTGTCGTCCGGCGGCTCGGATGCGGTCATCGTGCGTGAGTTCGATCTCGACGCGCAGCGTTTCGTCGAC
GATGGATTCGTGCTTCCCGCGCCGGGCAAGCATTCGATTTCGTGGATCGACCGTGACACCGTCTATCTCGGTTGGGACAA
CGGCGGGACGAGTCTGACGCGCTCCGGCTACCCGCGCGAAGTGCGGCGCTGGACGCGCGGCACACCGGTAGCGGACGCGC
CGGTCGTGTTCCGCGCTGAATTCGCCGACATTTCGGTCGATGCGTTTTACGATCCGATTGACCAGCGTCATACGCTGTCC
GTCGGCATCGACGCATTTGATTCGGACACCTACTATCTCGACGCGGCGCAGCGGTGGCAGCGCTTCGACGTACCGTCACA
CGTAGCAATCTGCGCGTGGCACGGGTGGCTGCTTCTCCAGCCACGCCTTCCCTGGGATTGCGGTGAAGCGACCTACGCGG
GCGGCGCGCTGCTGGCCATTCGCGAGGCGGCATTCCTGCGCGGCGAGCGGGATTGCGTGCCTCTGTTCGTGCCCACGCCG
ACAACGTCGGGCTGCGGCTACTCGTTCACGCGGCACCTGCTGATCGTTTCGTATCTCGACGACGTTCAGCAGCGCACCGT
TCTGTGGCAGGCAAAACCGTCGGGCGACGGCACCTGGCAGTGGGGGTCACGCGAGTTTCCATTGCAGGCGAACGCGCAAG
TCAATGTTTCGCCGATCGAGCCGGCCATAGGCGACGAGGCATATGTCAGCGTCGAAGACTATCTTCAGCCCGGTTGCTGC
TGGATCGTGGATCTCACGCAGGACGATCTCACGCAGTGGGAATTGATCGACCGTTACCCTGCCGATTTCGATGCGACGCC
CTTCGCGGTGACGCGCGCTCACGCGGTGTCGGCAGACGGCACCCGAGTGCCCTACACGATGATCGGACCACGTGAGCCAG
AACCGCAAACGCGGCCATGTCTGCTGACCGGCTATGGCGGCTTTGCGATTCCGCTGACGCCAGGCTACCTGGGCGGGACC
GGCGCCGGATGGCTGGCGGCCGGCGGTGTCTACGTGGTCGCGCATATTCGTGGCGGCGGGGAGTTCGGACCCGCCTGGCA
TACTGCGGCGGTGGGTCCAAACCGCCAGCGTGCATTCGACGACTTCATCGCGGTCGCGCAGGCGCTGATTGACACGGGGG
TGACGACGCCTGCGCAGCTCGGCATACAGGGCGGCAGCAACGGCGGCTTACTGGTGGCGGCATGCATGATGCAACGGCCC
GATCTGTTCGGCGCCGTGGTAAGCCGGGTGCCGCTTCTGGATATGAGCCGCTACCATCTCCTGCATGCAGGGGCGTCATG
GCTGGAAGAATACGGCGATCCGGATATCCTCGAAGACGCGCGGGTGCTCGCTGCGTATTCGCCTTACCACCAGGTGTCGG
CCAGCCGGGCGTACCCGCCCGTGCTATTCACGACAGCCACCGGCGACGACCGCGTGCATCCCGGCCATGCGCGCAAGATG
GCCGCGCGAATGCAGGCGCTCGGCGCCGGCAAGGTCTGGTACTTCGAGGACCTGCAGGAGGGCGGCCACGGGGCCGCAGA
CGGGCTGCACATGGTGGGTCAGGGCGCTCTCGTCTTCGAGTTCCTGTGGTCTTGCCTGGGTGACGGTGAGCGGCGCACCG
CCTGA

Upstream 100 bases:

>100_bases
TGTGTGTCCGGCACGCATGGTGCGGCATGGTGTCACCTTAACGAGGCCGCATGCAGAGGGAGCGGCATAATCGGTTGTCC
TTCCTATTGCGGGCACCGTT

Downstream 100 bases:

>100_bases
GCGCATGCGATCCGGCGGCCGTCAGCCGAGTTGCGCGACCACCGGCGCGTGGTCCGACGGCTGATCCCACTTGCGCGGCA
CCTTGTCGACCTCGCACGAC

Product: prolyl oligopeptidase family protein

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MSTPFCWPLSPDTFIALEALDDPLVLEWVERQNVRTLAAWASGARFDTLKDRLASVYLPRDRPVVPTRWREWAHDIWQDG
HNPKGIWRRASWANWRAGQAVWQTLLDFDALGEDEQMPWVCVERQILYPDGDRALISLSSGGSDAVIVREFDLDAQRFVD
DGFVLPAPGKHSISWIDRDTVYLGWDNGGTSLTRSGYPREVRRWTRGTPVADAPVVFRAEFADISVDAFYDPIDQRHTLS
VGIDAFDSDTYYLDAAQRWQRFDVPSHVAICAWHGWLLLQPRLPWDCGEATYAGGALLAIREAAFLRGERDCVPLFVPTP
TTSGCGYSFTRHLLIVSYLDDVQQRTVLWQAKPSGDGTWQWGSREFPLQANAQVNVSPIEPAIGDEAYVSVEDYLQPGCC
WIVDLTQDDLTQWELIDRYPADFDATPFAVTRAHAVSADGTRVPYTMIGPREPEPQTRPCLLTGYGGFAIPLTPGYLGGT
GAGWLAAGGVYVVAHIRGGGEFGPAWHTAAVGPNRQRAFDDFIAVAQALIDTGVTTPAQLGIQGGSNGGLLVAACMMQRP
DLFGAVVSRVPLLDMSRYHLLHAGASWLEEYGDPDILEDARVLAAYSPYHQVSASRAYPPVLFTTATGDDRVHPGHARKM
AARMQALGAGKVWYFEDLQEGGHGAADGLHMVGQGALVFEFLWSCLGDGERRTA

Sequences:

>Translated_694_residues
MSTPFCWPLSPDTFIALEALDDPLVLEWVERQNVRTLAAWASGARFDTLKDRLASVYLPRDRPVVPTRWREWAHDIWQDG
HNPKGIWRRASWANWRAGQAVWQTLLDFDALGEDEQMPWVCVERQILYPDGDRALISLSSGGSDAVIVREFDLDAQRFVD
DGFVLPAPGKHSISWIDRDTVYLGWDNGGTSLTRSGYPREVRRWTRGTPVADAPVVFRAEFADISVDAFYDPIDQRHTLS
VGIDAFDSDTYYLDAAQRWQRFDVPSHVAICAWHGWLLLQPRLPWDCGEATYAGGALLAIREAAFLRGERDCVPLFVPTP
TTSGCGYSFTRHLLIVSYLDDVQQRTVLWQAKPSGDGTWQWGSREFPLQANAQVNVSPIEPAIGDEAYVSVEDYLQPGCC
WIVDLTQDDLTQWELIDRYPADFDATPFAVTRAHAVSADGTRVPYTMIGPREPEPQTRPCLLTGYGGFAIPLTPGYLGGT
GAGWLAAGGVYVVAHIRGGGEFGPAWHTAAVGPNRQRAFDDFIAVAQALIDTGVTTPAQLGIQGGSNGGLLVAACMMQRP
DLFGAVVSRVPLLDMSRYHLLHAGASWLEEYGDPDILEDARVLAAYSPYHQVSASRAYPPVLFTTATGDDRVHPGHARKM
AARMQALGAGKVWYFEDLQEGGHGAADGLHMVGQGALVFEFLWSCLGDGERRTA
>Mature_693_residues
STPFCWPLSPDTFIALEALDDPLVLEWVERQNVRTLAAWASGARFDTLKDRLASVYLPRDRPVVPTRWREWAHDIWQDGH
NPKGIWRRASWANWRAGQAVWQTLLDFDALGEDEQMPWVCVERQILYPDGDRALISLSSGGSDAVIVREFDLDAQRFVDD
GFVLPAPGKHSISWIDRDTVYLGWDNGGTSLTRSGYPREVRRWTRGTPVADAPVVFRAEFADISVDAFYDPIDQRHTLSV
GIDAFDSDTYYLDAAQRWQRFDVPSHVAICAWHGWLLLQPRLPWDCGEATYAGGALLAIREAAFLRGERDCVPLFVPTPT
TSGCGYSFTRHLLIVSYLDDVQQRTVLWQAKPSGDGTWQWGSREFPLQANAQVNVSPIEPAIGDEAYVSVEDYLQPGCCW
IVDLTQDDLTQWELIDRYPADFDATPFAVTRAHAVSADGTRVPYTMIGPREPEPQTRPCLLTGYGGFAIPLTPGYLGGTG
AGWLAAGGVYVVAHIRGGGEFGPAWHTAAVGPNRQRAFDDFIAVAQALIDTGVTTPAQLGIQGGSNGGLLVAACMMQRPD
LFGAVVSRVPLLDMSRYHLLHAGASWLEEYGDPDILEDARVLAAYSPYHQVSASRAYPPVLFTTATGDDRVHPGHARKMA
ARMQALGAGKVWYFEDLQEGGHGAADGLHMVGQGALVFEFLWSCLGDGERRTA

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=367, Percent_Identity=34.0599455040872, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI284172420, Length=212, Percent_Identity=29.2452830188679, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI284172413, Length=212, Percent_Identity=29.2452830188679, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI70778815, Length=212, Percent_Identity=29.2452830188679, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI284172438, Length=212, Percent_Identity=29.2452830188679, Blast_Score=74, Evalue=6e-13,
Organism=Homo sapiens, GI284172431, Length=212, Percent_Identity=29.2452830188679, Blast_Score=74, Evalue=6e-13,
Organism=Homo sapiens, GI108860686, Length=212, Percent_Identity=29.2452830188679, Blast_Score=74, Evalue=6e-13,
Organism=Homo sapiens, GI108860692, Length=204, Percent_Identity=28.4313725490196, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788150, Length=279, Percent_Identity=28.3154121863799, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583414, Length=346, Percent_Identity=36.9942196531792, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI221510989, Length=368, Percent_Identity=31.5217391304348, Blast_Score=175, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 76653; Mature: 76521

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPFCWPLSPDTFIALEALDDPLVLEWVERQNVRTLAAWASGARFDTLKDRLASVYLPR
CCCCEECCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEHHHCCCCHHHHHHHHHHEECCC
DRPVVPTRWREWAHDIWQDGHNPKGIWRRASWANWRAGQAVWQTLLDFDALGEDEQMPWV
CCCCCCHHHHHHHHHHHHCCCCCCHHEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
CVERQILYPDGDRALISLSSGGSDAVIVREFDLDAQRFVDDGFVLPAPGKHSISWIDRDT
EECCEEECCCCCEEEEEEECCCCCEEEEEEECCCHHHHCCCCEEECCCCCCCEEEEECCE
VYLGWDNGGTSLTRSGYPREVRRWTRGTPVADAPVVFRAEFADISVDAFYDPIDQRHTLS
EEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEECCEEEHHHHCCCCCCEEEE
VGIDAFDSDTYYLDAAQRWQRFDVPSHVAICAWHGWLLLQPRLPWDCGEATYAGGALLAI
EECEEECCCCEEEEHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCEEEEH
REAAFLRGERDCVPLFVPTPTTSGCGYSFTRHLLIVSYLDDVQQRTVLWQAKPSGDGTWQ
HHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCC
WGSREFPLQANAQVNVSPIEPAIGDEAYVSVEDYLQPGCCWIVDLTQDDLTQWELIDRYP
CCCCCCCEECCCEEEECCCCCCCCCCEEEEHHHHCCCCEEEEEECCCCCCHHHHHHHCCC
ADFDATPFAVTRAHAVSADGTRVPYTMIGPREPEPQTRPCLLTGYGGFAIPLTPGYLGGT
CCCCCCCEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCEEEECCCCEEEECCCCCCCCC
GAGWLAAGGVYVVAHIRGGGEFGPAWHTAAVGPNRQRAFDDFIAVAQALIDTGVTTPAQL
CCCHHHCCCEEEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHE
GIQGGSNGGLLVAACMMQRPDLFGAVVSRVPLLDMSRYHLLHAGASWLEEYGDPDILEDA
EECCCCCCCCEEHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCC
RVLAAYSPYHQVSASRAYPPVLFTTATGDDRVHPGHARKMAARMQALGAGKVWYFEDLQE
HHEEECCCHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHC
GGHGAADGLHMVGQGALVFEFLWSCLGDGERRTA
CCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STPFCWPLSPDTFIALEALDDPLVLEWVERQNVRTLAAWASGARFDTLKDRLASVYLPR
CCCEECCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEHHHCCCCHHHHHHHHHHEECCC
DRPVVPTRWREWAHDIWQDGHNPKGIWRRASWANWRAGQAVWQTLLDFDALGEDEQMPWV
CCCCCCHHHHHHHHHHHHCCCCCCHHEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
CVERQILYPDGDRALISLSSGGSDAVIVREFDLDAQRFVDDGFVLPAPGKHSISWIDRDT
EECCEEECCCCCEEEEEEECCCCCEEEEEEECCCHHHHCCCCEEECCCCCCCEEEEECCE
VYLGWDNGGTSLTRSGYPREVRRWTRGTPVADAPVVFRAEFADISVDAFYDPIDQRHTLS
EEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEECCEEEHHHHCCCCCCEEEE
VGIDAFDSDTYYLDAAQRWQRFDVPSHVAICAWHGWLLLQPRLPWDCGEATYAGGALLAI
EECEEECCCCEEEEHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCEEEEH
REAAFLRGERDCVPLFVPTPTTSGCGYSFTRHLLIVSYLDDVQQRTVLWQAKPSGDGTWQ
HHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCC
WGSREFPLQANAQVNVSPIEPAIGDEAYVSVEDYLQPGCCWIVDLTQDDLTQWELIDRYP
CCCCCCCEECCCEEEECCCCCCCCCCEEEEHHHHCCCCEEEEEECCCCCCHHHHHHHCCC
ADFDATPFAVTRAHAVSADGTRVPYTMIGPREPEPQTRPCLLTGYGGFAIPLTPGYLGGT
CCCCCCCEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCEEEECCCCEEEECCCCCCCCC
GAGWLAAGGVYVVAHIRGGGEFGPAWHTAAVGPNRQRAFDDFIAVAQALIDTGVTTPAQL
CCCHHHCCCEEEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHE
GIQGGSNGGLLVAACMMQRPDLFGAVVSRVPLLDMSRYHLLHAGASWLEEYGDPDILEDA
EECCCCCCCCEEHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCC
RVLAAYSPYHQVSASRAYPPVLFTTATGDDRVHPGHARKMAARMQALGAGKVWYFEDLQE
HHEEECCCHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHC
GGHGAADGLHMVGQGALVFEFLWSCLGDGERRTA
CCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]