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The map label for this gene is purA [H]

Identifier: 91784195

GI number: 91784195

Start: 3139504

End: 3140850

Strand: Reverse

Name: purA [H]

Synonym: Bxe_A1606

Alternate gene names: 91784195

Gene position: 3140850-3139504 (Counterclockwise)

Preceding gene: 91784196

Following gene: 91784194

Centisome position: 64.15

GC content: 62.81

Gene sequence:

>1347_bases
ATGTCTGCCAGCGCAGTGAATGTGAACCCCGGGCGTAACGTCGTCGTCGTGGGGACCCAATGGGGTGATGAAGGCAAGGG
CAAGATCGTCGACTGGCTGACGGACCACGCTCAAGGCGTCGTTCGCTTCCAGGGCGGTCACAATGCCGGTCACACGCTTA
TCATCGGCGGCAAGAAAACCATCTTGCGTCTGATTCCGTCGGGCATCATGCATCCCGGCGTCGCGTGCTACATCGGCAAT
GGCGTCGTGTTGTCGCCGGAAGCGCTGTTCAAGGAAATCGGCGAGCTCGAAGCCGCCGGGGTCGATGTCCAGAATCGCCT
CTTCATTTCCGAAGCCACCACGCTGATCCTGCCGTATCACATCGCCATCGACCAGGGCCGCGAAGCGCGCCGTGGCGCAG
GCAAGATCGGCACCACCGGCCGCGGCATCGGCCCGGCTTACGAAGACAAGGTGGCACGCCGCGGTTTGCGCGTGCAGGAC
CTGTTCGAGCCGGAGATCTTCGCCGAACGTCTGCGTGAAAACCTCGACTATCACAACTTCGTGTTGACGCAATACCTGGG
CGTCGCCGCTGTCGACTTCCAGCAAACGCTCGACACGATGCTCAGCTATGCCGACCGTCTGAGGCCGATGGTGACCGACG
TGTCGCGCCGTCTGTACGACGTCAACGCCGCCGGCAGCAACCTGCTGTTCGAAGGCGCGCAAGGCACGCTGCTCGACATC
GATCACGGCACGTATCCGTTCGTCACGTCGAGCAACTGCGTGGCCGGTGCGGCCACGGCCGGCGCGGGTGTCGGTCCGCA
AAAGCTGAACTACATTCTCGGCATCACCAAGGCGTACTGCACGCGCGTCGGCTCGGGCCCGTTCCCGAGCGAACTGTACG
ACGCGGATAACGCTGCACGCCAGGAAGCGATCGGCCTCGAACTGGCTACCGTCGGCAAGGAATTCGGCTCGGTCACGGGT
CGTCCGCGCCGCACCGGCTGGCTCGACGCGGCCGCGCTGCGCCGCTCGATCCAGATCAACGGCGTATCGGGTCTGTGCAT
GACCAAGCTGGACGTGCTCGACGGTCTTGACGAAGTGAAACTGTGCGTTGGTTACAAGGTGGACGGCAAGAATGTCGACC
TGCTGCCGCGCGGCGCGTCGGAAGTTGCACGCTGTGAGCCGGTGTACGAAACCTTCGCGGGCTGGAAGGAAAGCACGGTT
GGCATCAAGGAATGGGACAAACTGCCGGCGAATGCGCGCGCGTATCTGTCGCGTGTGCAGGAGGTTGCGGGCATTCCGAT
CGACATGGTGTCGACCGGTCCGGATCGCGATGAAACCATCCTGCTTCGTCATCCGTTCAAGGTTTAA

Upstream 100 bases:

>100_bases
ATACCCACCATAACGTGCATACCGTTGAGCGGAAACGGAAAAATTCGGAAGGTTCCGCGAACATAGGTAGAATACGTTTT
TAACCAGCTTACGAAACAAC

Downstream 100 bases:

>100_bases
ACATGGTGCAAGGTGTTCCCATGATTGCGATGAAAGATCCGCGCAACGACGACAAGAACCTCTGGGTCGGCTGGGACGAG
TATCACCGGTTGATCGAACT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMHPGVACYIGN
GVVLSPEALFKEIGELEAAGVDVQNRLFISEATTLILPYHIAIDQGREARRGAGKIGTTGRGIGPAYEDKVARRGLRVQD
LFEPEIFAERLRENLDYHNFVLTQYLGVAAVDFQQTLDTMLSYADRLRPMVTDVSRRLYDVNAAGSNLLFEGAQGTLLDI
DHGTYPFVTSSNCVAGAATAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNAARQEAIGLELATVGKEFGSVTG
RPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVKLCVGYKVDGKNVDLLPRGASEVARCEPVYETFAGWKESTV
GIKEWDKLPANARAYLSRVQEVAGIPIDMVSTGPDRDETILLRHPFKV

Sequences:

>Translated_448_residues
MSASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMHPGVACYIGN
GVVLSPEALFKEIGELEAAGVDVQNRLFISEATTLILPYHIAIDQGREARRGAGKIGTTGRGIGPAYEDKVARRGLRVQD
LFEPEIFAERLRENLDYHNFVLTQYLGVAAVDFQQTLDTMLSYADRLRPMVTDVSRRLYDVNAAGSNLLFEGAQGTLLDI
DHGTYPFVTSSNCVAGAATAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNAARQEAIGLELATVGKEFGSVTG
RPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVKLCVGYKVDGKNVDLLPRGASEVARCEPVYETFAGWKESTV
GIKEWDKLPANARAYLSRVQEVAGIPIDMVSTGPDRDETILLRHPFKV
>Mature_447_residues
SASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMHPGVACYIGNG
VVLSPEALFKEIGELEAAGVDVQNRLFISEATTLILPYHIAIDQGREARRGAGKIGTTGRGIGPAYEDKVARRGLRVQDL
FEPEIFAERLRENLDYHNFVLTQYLGVAAVDFQQTLDTMLSYADRLRPMVTDVSRRLYDVNAAGSNLLFEGAQGTLLDID
HGTYPFVTSSNCVAGAATAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNAARQEAIGLELATVGKEFGSVTGR
PRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVKLCVGYKVDGKNVDLLPRGASEVARCEPVYETFAGWKESTVG
IKEWDKLPANARAYLSRVQEVAGIPIDMVSTGPDRDETILLRHPFKV

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=437, Percent_Identity=42.3340961098398, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI34577063, Length=446, Percent_Identity=39.6860986547085, Blast_Score=305, Evalue=5e-83,
Organism=Homo sapiens, GI40316944, Length=405, Percent_Identity=40.4938271604938, Blast_Score=290, Evalue=2e-78,
Organism=Escherichia coli, GI1790620, Length=437, Percent_Identity=59.7254004576659, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI25146553, Length=439, Percent_Identity=40.5466970387244, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI25146555, Length=436, Percent_Identity=40.5963302752294, Blast_Score=316, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=437, Percent_Identity=40.045766590389, Blast_Score=322, Evalue=1e-88,
Organism=Drosophila melanogaster, GI21356033, Length=456, Percent_Identity=39.0350877192982, Blast_Score=314, Evalue=8e-86,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 48325; Mature: 48193

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKT
CCCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHH
ILRLIPSGIMHPGVACYIGNGVVLSPEALFKEIGELEAAGVDVQNRLFISEATTLILPYH
HHHHHHHHCCCCCEEEEECCCEEECHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEEEEE
IAIDQGREARRGAGKIGTTGRGIGPAYEDKVARRGLRVQDLFEPEIFAERLRENLDYHNF
EEECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCHHHH
VLTQYLGVAAVDFQQTLDTMLSYADRLRPMVTDVSRRLYDVNAAGSNLLFEGAQGTLLDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCEEEC
DHGTYPFVTSSNCVAGAATAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNAAR
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHH
QEAIGLELATVGKEFGSVTGRPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVK
HHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEE
LCVGYKVDGKNVDLLPRGASEVARCEPVYETFAGWKESTVGIKEWDKLPANARAYLSRVQ
EEEEEEECCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHH
EVAGIPIDMVSTGPDRDETILLRHPFKV
HHHCCCEEEECCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKT
CCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHH
ILRLIPSGIMHPGVACYIGNGVVLSPEALFKEIGELEAAGVDVQNRLFISEATTLILPYH
HHHHHHHHCCCCCEEEEECCCEEECHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEEEEE
IAIDQGREARRGAGKIGTTGRGIGPAYEDKVARRGLRVQDLFEPEIFAERLRENLDYHNF
EEECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCHHHH
VLTQYLGVAAVDFQQTLDTMLSYADRLRPMVTDVSRRLYDVNAAGSNLLFEGAQGTLLDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCEEEC
DHGTYPFVTSSNCVAGAATAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNAAR
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHH
QEAIGLELATVGKEFGSVTGRPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVK
HHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEE
LCVGYKVDGKNVDLLPRGASEVARCEPVYETFAGWKESTVGIKEWDKLPANARAYLSRVQ
EEEEEEECCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHH
EVAGIPIDMVSTGPDRDETILLRHPFKV
HHHCCCEEEECCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA