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The map label for this gene is ydaP [H]

Identifier: 91783973

GI number: 91783973

Start: 2883066

End: 2884907

Strand: Direct

Name: ydaP [H]

Synonym: Bxe_A1836

Alternate gene names: 91783973

Gene position: 2883066-2884907 (Clockwise)

Preceding gene: 91783972

Following gene: 91783975

Centisome position: 58.89

GC content: 64.39

Gene sequence:

>1842_bases
ATGTCTCAAACCGTCGGCGATTTCATCATTGAACGACTTCACGCCTGGGGCGTGCGCCGCATCTATGGCTACCCCGGCGA
CGGCATCAACGGCGTTTTCGGCGCGCTCAACCGCGCGCAAACCGAAGCCGCCAGGCACCGCAAAAAAGGATCCGACGACA
GCGCGCACGCGATCGAATTCGTCCAGGTTCGCCACGAGGAAATGGCCGCCTTCATGGCGGCGGCCCATGCAAAATTCACC
GGCGAGCTTGGCGTATGCATCGCGACCTCCGGCCCCGGCGCCTCGCATCTGCTGACCGGACTGTACGACGCTCGCATGGA
CCATATGCCCGTGCTCGCCATAGCGGGCCAGCAGGCGCGCGCCGCGCTCGGCGGTCACTATCAGCAGGAGCTCGACCTCG
TCTCCATGTTCAAGGACGTGGCCGGAGCTTTCGTCGAACAGGCTACGGTGCCCGCGCAAGTGCGCCATCTGGTCGACCGC
GCTGTGCGCACGGCACTTTCCGAACGCAAGGTCACGGCGCTGATCCTGCCCAATGATCTGCAAGATCTGCCCTACGAAGC
ACCCGGACGCAAGCACGGCACGCTGCATTCGGGCGTGGGTTATCGCGCGCCGCGCCTCGTGCCCTACCCCGACGATCTTC
AGCAAGCCGCCGATATCCTGAACGCCGGCAAGAAAGTGGCGATCCTCGTCGGCGCGGGCGGCCTGCACGCCACCGACGAA
CTGATCGCCGTCGCCGAAAAGCTCGGTGCGGGGGTCGCGAAGGCGCTGCTCGGCAAAGCCGCGCTGCCCGACGATCTGCC
GTGGGTGACCGGCTCGATCGGACTGCTCGGCACCAAACCCAGCTACGACCTGATGGCCGAATGCGATACGCTGCTGATGA
TCGGCTCCGGTTTTCCGTACGCGGAATTTCTGCCGAAGGAAGGCGCGGCGCGCGGCGTGCAGATCGACATCAAGGCCGAC
ATGCTGAGCCTGCGTTACCCGATGGAAGTGAATCTGGTCGGCGACAGTGCCGAAACCTTGCGCGCGCTGCTGCCGCTACT
CGAAGAGAAGAAGGATCGCGCGTGGCGCAAGCGGGTCGAAGGCTGGAGCGCAGACTGGTGGAAAACGCTCGACAAGCGTG
CGCACGAGCCGGGCAAGGACGCCGTCAATCCGCAGCTCACCGTGTGGGAACTGTCGAAGCGCATTGCGTCCAACGCGATC
GTCACGAGCGACTCCGGTTCGGTGGCGAACTGGTACGCGCGCGACCTGAAAGTGCAGCGCGGCATGATGTGTTCGCTCTC
GGGCGGACTGGCGTCGATGGGCGCCGCCGTGCCATATGCGATCGCCGCCAAATTCGCGTATCCGGAGCGCCCTGTGATTG
CGCTCGTCGGCGACGGCGCAATGCAGATGAACAATATGGCCGAGCTGATCACCGTGTCGAAGTACTGGCGAGGCTGGTCC
GATCCGCGCTGGATCTGCATGGTGCTGAACAACCAGGACCTCAACCAGGTGACGTGGGAACAGCGCGCGATGGAAGGCGA
TCCGAAGTTCGAAGCATCGCAGGACATTCCCTGCGTGCCGTATCACAAGTTCGCGGAACTGATCGGCCTCAAGGGCATCT
ACGTCGACGACGCGCAGCAGATGGGCGCCGCCTGGGACGATGCGCTCGCCGCCGACCGCCCGGTGGTCATCGAAGTGAAG
GCCGATCCCAACATCGCGCCGCTGCCGCCGCATATCACGCTGGCCCAGGCGAAGGCGTTTGCATCGACGCTGATGAAGGG
CGATCCCAACGAAGGCAATGTGATAGTCGAGACGGCGAAGCAGGTGCTCGACGCGGTGCTGCCGGGCCATCACAGCAAGT
GA

Upstream 100 bases:

>100_bases
GGACCCGGATCAGCTTACGCCACGCCTGCAACGGCAGGGAACAGCGCTTGCTCGCCGTTGCTTCGACCACCCTCATGCAC
CGTGTACGAAGGAGAGCCTG

Downstream 100 bases:

>100_bases
GCGCGGCGGCGCCCGCCGAAGTGCCGAAGGCTATTGACGCAATTCCTCCAGCACCGCGCTGAGATCCTCCAGCGCGTAAG
GCTTTCGCAGCGCCTTCCAG

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MSQTVGDFIIERLHAWGVRRIYGYPGDGINGVFGALNRAQTEAARHRKKGSDDSAHAIEFVQVRHEEMAAFMAAAHAKFT
GELGVCIATSGPGASHLLTGLYDARMDHMPVLAIAGQQARAALGGHYQQELDLVSMFKDVAGAFVEQATVPAQVRHLVDR
AVRTALSERKVTALILPNDLQDLPYEAPGRKHGTLHSGVGYRAPRLVPYPDDLQQAADILNAGKKVAILVGAGGLHATDE
LIAVAEKLGAGVAKALLGKAALPDDLPWVTGSIGLLGTKPSYDLMAECDTLLMIGSGFPYAEFLPKEGAARGVQIDIKAD
MLSLRYPMEVNLVGDSAETLRALLPLLEEKKDRAWRKRVEGWSADWWKTLDKRAHEPGKDAVNPQLTVWELSKRIASNAI
VTSDSGSVANWYARDLKVQRGMMCSLSGGLASMGAAVPYAIAAKFAYPERPVIALVGDGAMQMNNMAELITVSKYWRGWS
DPRWICMVLNNQDLNQVTWEQRAMEGDPKFEASQDIPCVPYHKFAELIGLKGIYVDDAQQMGAAWDDALAADRPVVIEVK
ADPNIAPLPPHITLAQAKAFASTLMKGDPNEGNVIVETAKQVLDAVLPGHHSK

Sequences:

>Translated_613_residues
MSQTVGDFIIERLHAWGVRRIYGYPGDGINGVFGALNRAQTEAARHRKKGSDDSAHAIEFVQVRHEEMAAFMAAAHAKFT
GELGVCIATSGPGASHLLTGLYDARMDHMPVLAIAGQQARAALGGHYQQELDLVSMFKDVAGAFVEQATVPAQVRHLVDR
AVRTALSERKVTALILPNDLQDLPYEAPGRKHGTLHSGVGYRAPRLVPYPDDLQQAADILNAGKKVAILVGAGGLHATDE
LIAVAEKLGAGVAKALLGKAALPDDLPWVTGSIGLLGTKPSYDLMAECDTLLMIGSGFPYAEFLPKEGAARGVQIDIKAD
MLSLRYPMEVNLVGDSAETLRALLPLLEEKKDRAWRKRVEGWSADWWKTLDKRAHEPGKDAVNPQLTVWELSKRIASNAI
VTSDSGSVANWYARDLKVQRGMMCSLSGGLASMGAAVPYAIAAKFAYPERPVIALVGDGAMQMNNMAELITVSKYWRGWS
DPRWICMVLNNQDLNQVTWEQRAMEGDPKFEASQDIPCVPYHKFAELIGLKGIYVDDAQQMGAAWDDALAADRPVVIEVK
ADPNIAPLPPHITLAQAKAFASTLMKGDPNEGNVIVETAKQVLDAVLPGHHSK
>Mature_612_residues
SQTVGDFIIERLHAWGVRRIYGYPGDGINGVFGALNRAQTEAARHRKKGSDDSAHAIEFVQVRHEEMAAFMAAAHAKFTG
ELGVCIATSGPGASHLLTGLYDARMDHMPVLAIAGQQARAALGGHYQQELDLVSMFKDVAGAFVEQATVPAQVRHLVDRA
VRTALSERKVTALILPNDLQDLPYEAPGRKHGTLHSGVGYRAPRLVPYPDDLQQAADILNAGKKVAILVGAGGLHATDEL
IAVAEKLGAGVAKALLGKAALPDDLPWVTGSIGLLGTKPSYDLMAECDTLLMIGSGFPYAEFLPKEGAARGVQIDIKADM
LSLRYPMEVNLVGDSAETLRALLPLLEEKKDRAWRKRVEGWSADWWKTLDKRAHEPGKDAVNPQLTVWELSKRIASNAIV
TSDSGSVANWYARDLKVQRGMMCSLSGGLASMGAAVPYAIAAKFAYPERPVIALVGDGAMQMNNMAELITVSKYWRGWSD
PRWICMVLNNQDLNQVTWEQRAMEGDPKFEASQDIPCVPYHKFAELIGLKGIYVDDAQQMGAAWDDALAADRPVVIEVKA
DPNIAPLPPHITLAQAKAFASTLMKGDPNEGNVIVETAKQVLDAVLPGHHSK

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=546, Percent_Identity=21.6117216117216, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1787096, Length=605, Percent_Identity=33.8842975206612, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI87081685, Length=583, Percent_Identity=27.7873070325901, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1790104, Length=577, Percent_Identity=27.0363951473137, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1786717, Length=577, Percent_Identity=26.8630849220104, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1788716, Length=583, Percent_Identity=22.9845626072041, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17531301, Length=552, Percent_Identity=25.5434782608696, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=579, Percent_Identity=25.2158894645941, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323755, Length=593, Percent_Identity=24.9578414839798, Blast_Score=139, Evalue=9e-34,
Organism=Drosophila melanogaster, GI19922626, Length=555, Percent_Identity=25.5855855855856, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 66364; Mature: 66233

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTVGDFIIERLHAWGVRRIYGYPGDGINGVFGALNRAQTEAARHRKKGSDDSAHAIEF
CCCCHHHHHHHHHHHHCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VQVRHEEMAAFMAAAHAKFTGELGVCIATSGPGASHLLTGLYDARMDHMPVLAIAGQQAR
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCHHH
AALGGHYQQELDLVSMFKDVAGAFVEQATVPAQVRHLVDRAVRTALSERKVTALILPNDL
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCH
QDLPYEAPGRKHGTLHSGVGYRAPRLVPYPDDLQQAADILNAGKKVAILVGAGGLHATDE
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHH
LIAVAEKLGAGVAKALLGKAALPDDLPWVTGSIGLLGTKPSYDLMAECDTLLMIGSGFPY
HHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCEEEEEECCCCH
AEFLPKEGAARGVQIDIKADMLSLRYPMEVNLVGDSAETLRALLPLLEEKKDRAWRKRVE
HHHCCCCCCCCCEEEEEEECCEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
GWSADWWKTLDKRAHEPGKDAVNPQLTVWELSKRIASNAIVTSDSGSVANWYARDLKVQR
CCCCHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHC
GMMCSLSGGLASMGAAVPYAIAAKFAYPERPVIALVGDGAMQMNNMAELITVSKYWRGWS
CCEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCC
DPRWICMVLNNQDLNQVTWEQRAMEGDPKFEASQDIPCVPYHKFAELIGLKGIYVDDAQQ
CCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCHHH
MGAAWDDALAADRPVVIEVKADPNIAPLPPHITLAQAKAFASTLMKGDPNEGNVIVETAK
HCCCHHHHHCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCEEHHHHH
QVLDAVLPGHHSK
HHHHHHCCCCCCC
>Mature Secondary Structure 
SQTVGDFIIERLHAWGVRRIYGYPGDGINGVFGALNRAQTEAARHRKKGSDDSAHAIEF
CCCHHHHHHHHHHHHCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VQVRHEEMAAFMAAAHAKFTGELGVCIATSGPGASHLLTGLYDARMDHMPVLAIAGQQAR
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCHHH
AALGGHYQQELDLVSMFKDVAGAFVEQATVPAQVRHLVDRAVRTALSERKVTALILPNDL
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCH
QDLPYEAPGRKHGTLHSGVGYRAPRLVPYPDDLQQAADILNAGKKVAILVGAGGLHATDE
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHH
LIAVAEKLGAGVAKALLGKAALPDDLPWVTGSIGLLGTKPSYDLMAECDTLLMIGSGFPY
HHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCEEEEEECCCCH
AEFLPKEGAARGVQIDIKADMLSLRYPMEVNLVGDSAETLRALLPLLEEKKDRAWRKRVE
HHHCCCCCCCCCEEEEEEECCEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
GWSADWWKTLDKRAHEPGKDAVNPQLTVWELSKRIASNAIVTSDSGSVANWYARDLKVQR
CCCCHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHC
GMMCSLSGGLASMGAAVPYAIAAKFAYPERPVIALVGDGAMQMNNMAELITVSKYWRGWS
CCEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCC
DPRWICMVLNNQDLNQVTWEQRAMEGDPKFEASQDIPCVPYHKFAELIGLKGIYVDDAQQ
CCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCHHH
MGAAWDDALAADRPVVIEVKADPNIAPLPPHITLAQAKAFASTLMKGDPNEGNVIVETAK
HCCCHHHHHCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCEEHHHHH
QVLDAVLPGHHSK
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0017 {lecithin}} 0.03 {sodium} 11 {pyruvate}} 10 {pyruvate}} 0.0022 {lysolecithin}} 2 {ferricyanide}} 0.0012 {thiamine} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]