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The map label for this gene is pldA [C]

Identifier: 91783863

GI number: 91783863

Start: 2781227

End: 2782585

Strand: Direct

Name: pldA [C]

Synonym: Bxe_A1948

Alternate gene names: 91783863

Gene position: 2781227-2782585 (Clockwise)

Preceding gene: 91783862

Following gene: 91783864

Centisome position: 56.81

GC content: 66.45

Gene sequence:

>1359_bases
ATGCGCTTTGACGCTTCAATCGACCGCGTTGCCCGCAATGCCGCTTCTCACGCTCGCCGCCCTGTTACGCGCTTTGCACG
CTTCGCGCGCTCCGGTCCACGCTTCGGCCGCGCCATGCTGCTGGCTGCGCTCGTTGCCGGCGCGGCGATGTCCGGCCAGG
CGCGCGCGACGGTGGCGATGCTGCAACCGCTTCGCGTGGCCGCCGCCAACGAGCCGTTGCAGATCACGATGCTCTACTCC
GCCGACGACGCCAAAGCGCTGACCGTCACTGTCCCGCCGACCATTCGCGTGAGCCTGAGCGCGGGCGAGCAGTCGCCGCA
GCCGCTCGACCTGCAACGCGAGCCGGGTGTGCCGGGCACGCTGCACCTGCGGCCGGGCCAGTTCCGCAAGGTGCGTTTCT
CGGCGCCGTGGCCGGAATCGGCGCGCGGCGAGGTACGCATCGACCCCGTGGGATTCGACGCATCGCCCGCGCTTGTCGCG
ATCAATCGCGGGCCGCAGCAGGACGCGATTGCGCAGGCAGAACGCACGGAAGGCCAGGCGAGCACGCCCGCGCAGGCTGC
CGCGACGGCGGCAATGGTCGATGGTCCGACCGGCGACGCCATCTCGCCGCCCGGCGACTCGCTCGCCACCACCGGACGCG
GCGTGCTCGCCCACCTCTCCTATTACGAGCCGATGTACATCGCCGCGGGCCACAACGGCGACACGAACGCGCGGCTGCAG
TTGAGCTTCAAATATCGGATCCGCATACCGGACGATCTGCGCTCGAAGGCGTTTCTCGACAACCTGTATTTCGCCTACAC
GCAGACTTCGATCTGGGATCTGTCCGCCGATTCGCGGCCGTTCCGCGACACCACCTACTCGCCGCAACTGTTCTACTACG
TGCCGGACACCGGCTGGAAGAGCCCGTTCTTCACCCGCATGGGCTTTGCAGCGGGCGTCGCGCACGAATCGAACGGCAAG
GCCGGCGCGGATTCGCGCAGCATCAACATGCCGTTTATCCGGCCGACGTGGGAATTCGGCGATCTGAGCGCCAACCATCT
GACCGTGTCGCCGAAGATCTATTACTACGTGGGGACGAGCAGCAACCCGGATATCGCCGACTATCGCGGCTACGTCGATC
TGCTGGTCAAATACGGCAGCCCGGACGGCTGGCAGCTGGCGACCACGCTGCGTAAAGGCACCAAACACTGGTACGGCAGC
GTGGATACGCAATTCACCTACCCGCTCGCCAGACTGCTGGGCAGCGCGTGGGGCGGCTATCTCTGGGTGGGCTACTTCAA
CGGCTATGGCGAGGATCTGCTCGACTACAACAATCGCCAGCACTGGATGGCGCGGATCGGCTACAGCATCGCGCGCTGA

Upstream 100 bases:

>100_bases
GCGCTTTCGCGCGATCGATCCGGTTCGCCCGGCATGTGTCGTGGTCCTACGCCTGCCGGCCTCCGTTTCGCTCGTTGCCC
TGCTCCCTGGACGACAACCC

Downstream 100 bases:

>100_bases
GATGCGCGGACATCGGCTGGGATGCAGGCCGCGCCGCCACGAGACAGGCCGCGGACGCCGCCAGACGCGCGCCAACGGCG
GCCTCGCCGTTGGCCACGGC

Product: putative outer membrane phospholipase A

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MRFDASIDRVARNAASHARRPVTRFARFARSGPRFGRAMLLAALVAGAAMSGQARATVAMLQPLRVAAANEPLQITMLYS
ADDAKALTVTVPPTIRVSLSAGEQSPQPLDLQREPGVPGTLHLRPGQFRKVRFSAPWPESARGEVRIDPVGFDASPALVA
INRGPQQDAIAQAERTEGQASTPAQAAATAAMVDGPTGDAISPPGDSLATTGRGVLAHLSYYEPMYIAAGHNGDTNARLQ
LSFKYRIRIPDDLRSKAFLDNLYFAYTQTSIWDLSADSRPFRDTTYSPQLFYYVPDTGWKSPFFTRMGFAAGVAHESNGK
AGADSRSINMPFIRPTWEFGDLSANHLTVSPKIYYYVGTSSNPDIADYRGYVDLLVKYGSPDGWQLATTLRKGTKHWYGS
VDTQFTYPLARLLGSAWGGYLWVGYFNGYGEDLLDYNNRQHWMARIGYSIAR

Sequences:

>Translated_452_residues
MRFDASIDRVARNAASHARRPVTRFARFARSGPRFGRAMLLAALVAGAAMSGQARATVAMLQPLRVAAANEPLQITMLYS
ADDAKALTVTVPPTIRVSLSAGEQSPQPLDLQREPGVPGTLHLRPGQFRKVRFSAPWPESARGEVRIDPVGFDASPALVA
INRGPQQDAIAQAERTEGQASTPAQAAATAAMVDGPTGDAISPPGDSLATTGRGVLAHLSYYEPMYIAAGHNGDTNARLQ
LSFKYRIRIPDDLRSKAFLDNLYFAYTQTSIWDLSADSRPFRDTTYSPQLFYYVPDTGWKSPFFTRMGFAAGVAHESNGK
AGADSRSINMPFIRPTWEFGDLSANHLTVSPKIYYYVGTSSNPDIADYRGYVDLLVKYGSPDGWQLATTLRKGTKHWYGS
VDTQFTYPLARLLGSAWGGYLWVGYFNGYGEDLLDYNNRQHWMARIGYSIAR
>Mature_452_residues
MRFDASIDRVARNAASHARRPVTRFARFARSGPRFGRAMLLAALVAGAAMSGQARATVAMLQPLRVAAANEPLQITMLYS
ADDAKALTVTVPPTIRVSLSAGEQSPQPLDLQREPGVPGTLHLRPGQFRKVRFSAPWPESARGEVRIDPVGFDASPALVA
INRGPQQDAIAQAERTEGQASTPAQAAATAAMVDGPTGDAISPPGDSLATTGRGVLAHLSYYEPMYIAAGHNGDTNARLQ
LSFKYRIRIPDDLRSKAFLDNLYFAYTQTSIWDLSADSRPFRDTTYSPQLFYYVPDTGWKSPFFTRMGFAAGVAHESNGK
AGADSRSINMPFIRPTWEFGDLSANHLTVSPKIYYYVGTSSNPDIADYRGYVDLLVKYGSPDGWQLATTLRKGTKHWYGS
VDTQFTYPLARLLGSAWGGYLWVGYFNGYGEDLLDYNNRQHWMARIGYSIAR

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=192, Percent_Identity=34.8958333333333, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 49597; Mature: 49597

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDASIDRVARNAASHARRPVTRFARFARSGPRFGRAMLLAALVAGAAMSGQARATVAM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
LQPLRVAAANEPLQITMLYSADDAKALTVTVPPTIRVSLSAGEQSPQPLDLQREPGVPGT
HCCHHHHCCCCCEEEEEEEECCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCEE
LHLRPGQFRKVRFSAPWPESARGEVRIDPVGFDASPALVAINRGPQQDAIAQAERTEGQA
EEECCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCC
STPAQAAATAAMVDGPTGDAISPPGDSLATTGRGVLAHLSYYEPMYIAAGHNGDTNARLQ
CCCHHHHHEEEEECCCCCCCCCCCCCCHHCCCCCCEEEECCCCCEEEEECCCCCCCCEEE
LSFKYRIRIPDDLRSKAFLDNLYFAYTQTSIWDLSADSRPFRDTTYSPQLFYYVPDTGWK
EEEEEEEECCHHHHHHHHHHHEEEEEECCEEEECCCCCCCCCCCCCCCEEEEECCCCCCC
SPFFTRMGFAAGVAHESNGKAGADSRSINMPFIRPTWEFGDLSANHLTVSPKIYYYVGTS
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEECCEEEEEEECC
SNPDIADYRGYVDLLVKYGSPDGWQLATTLRKGTKHWYGSVDTQFTYPLARLLGSAWGGY
CCCCHHHHCCEEEEEEECCCCCCHHHHHHHHHCCHHHCCCCCCEEHHHHHHHHHCCCCCE
LWVGYFNGYGEDLLDYNNRQHWMARIGYSIAR
EEEEEECCCCCHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRFDASIDRVARNAASHARRPVTRFARFARSGPRFGRAMLLAALVAGAAMSGQARATVAM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
LQPLRVAAANEPLQITMLYSADDAKALTVTVPPTIRVSLSAGEQSPQPLDLQREPGVPGT
HCCHHHHCCCCCEEEEEEEECCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCEE
LHLRPGQFRKVRFSAPWPESARGEVRIDPVGFDASPALVAINRGPQQDAIAQAERTEGQA
EEECCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCC
STPAQAAATAAMVDGPTGDAISPPGDSLATTGRGVLAHLSYYEPMYIAAGHNGDTNARLQ
CCCHHHHHEEEEECCCCCCCCCCCCCCHHCCCCCCEEEECCCCCEEEEECCCCCCCCEEE
LSFKYRIRIPDDLRSKAFLDNLYFAYTQTSIWDLSADSRPFRDTTYSPQLFYYVPDTGWK
EEEEEEEECCHHHHHHHHHHHEEEEEECCEEEECCCCCCCCCCCCCCCEEEEECCCCCCC
SPFFTRMGFAAGVAHESNGKAGADSRSINMPFIRPTWEFGDLSANHLTVSPKIYYYVGTS
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEECCEEEEEEECC
SNPDIADYRGYVDLLVKYGSPDGWQLATTLRKGTKHWYGSVDTQFTYPLARLLGSAWGGY
CCCCHHHHCCEEEEEEECCCCCCHHHHHHHHHCCHHHCCCCCCEEHHHHHHHHHCCCCCE
LWVGYFNGYGEDLLDYNNRQHWMARIGYSIAR
EEEEEECCCCCHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]