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The map label for this gene is potF [H]

Identifier: 91783861

GI number: 91783861

Start: 2778145

End: 2779245

Strand: Reverse

Name: potF [H]

Synonym: Bxe_A1950

Alternate gene names: 91783861

Gene position: 2779245-2778145 (Counterclockwise)

Preceding gene: 91783865

Following gene: 91783860

Centisome position: 56.77

GC content: 60.58

Gene sequence:

>1101_bases
ATGAAAAGACGGGTAGTGGGGCAAGTGGCGGCGCTGGTTCTGTGTGCGACGCCGTGGTTGACGGCCGCTGCGAAAGACAC
GCAACTGAACGTGTATAACTGGTCCGATTACATCGCCAAGGACACCATTCCCAACTTCACGAAGCAGACCGGCGTCCAGG
TCAGATACGACAACTACGACAGCGACGACACGCTGCAAGCCAAGCTCCTCACCGGCAATTCCGGCTACGACATCGTCGTG
CCCACCAGCAATTACGCCGGCAAGCAGATCGCCGCCGGCATCTTCGCACCGCTCGACAAATCGAAGCTGCCTAACCTGAA
ATACCTCGATCCTTCGCTGATGGCGCTCGTCGCGGGCGCCGATCCCGGCAACAAGTTTACCGTGCCCTGGGCATACGGCA
CGACCGGCCTTGGCTACAACGTCACCAAAGCGCAGCAGATCCTCGGCAAGAACGTGCCGCTCGACAGCTGGGACGTGCTC
TTCAAACCGGAAAACATCTCGAAGCTGAAGGCCTGCGGCGTGTCGGTGCTCGACGCGCCGGACCAGATGTTCGCCGCCGC
GCTGCACTACATCGGCAAGGATCCGATGAGCACGAATCCGGCCGACTACCGCGCCGCGCTCGAGATGATGAAGAAGATCC
GCCCGTATATCACGCAGTTCAACTCGTCGGGCTATATCAACGACCTGGTGGGCGGCGACGTGTGCTTCGCGTACGGCTGG
TCCGGCGACATTGTGATCGCCAAGCATCGCGCGGTCGAAGCGAAGAAGGCCTACAAGGTCGAGTATTACATTCCGAAGGG
CGGCGCACCGGTATGGTTCGACGTGATGGCGATTCCGAAGGACGCGAAGAACAAGGAAGCCGCGCTCGAGTGGATCAACT
ACATCGAAACGCCGCAGGTTCACGCCGCGATCACCAACGCCGTGTACTATCCGAGCGCCAACCTCGAAGCGCGCAAATAC
GTGGACAAGGACGTGGCGAACGACCCGGCAGTCTATCCGCCGCCTGAAGTCATCAAGACCCTGTTCCTCCTCAAGCCGCT
GCCGCCGGAAATCCAGCGGCTGCAAACGCGGCTGTGGACTGAGTTCAAGTCCGGCCGCTGA

Upstream 100 bases:

>100_bases
AATAATTGATGGTACTTTGCGGTTTTTTCAGGGGAGCCGCGCGCTGTGGCGCGGCGTAGGTGAAGACGGTCGGCTCGGCC
GGTCAGGATCGGAGAACGGG

Downstream 100 bases:

>100_bases
CTCGCGGCCGGTGTGAGCGGGTAAGCATCATCATGAAGCCCTCGGTGTTCACCGGGGGCTTTGTTCGTCAAACGCCGGAG
TAAAGCATTGTGATGAGTAG

Product: ABC polyamine transporter, periplasmic ligand binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKRRVVGQVAALVLCATPWLTAAAKDTQLNVYNWSDYIAKDTIPNFTKQTGVQVRYDNYDSDDTLQAKLLTGNSGYDIVV
PTSNYAGKQIAAGIFAPLDKSKLPNLKYLDPSLMALVAGADPGNKFTVPWAYGTTGLGYNVTKAQQILGKNVPLDSWDVL
FKPENISKLKACGVSVLDAPDQMFAAALHYIGKDPMSTNPADYRAALEMMKKIRPYITQFNSSGYINDLVGGDVCFAYGW
SGDIVIAKHRAVEAKKAYKVEYYIPKGGAPVWFDVMAIPKDAKNKEAALEWINYIETPQVHAAITNAVYYPSANLEARKY
VDKDVANDPAVYPPPEVIKTLFLLKPLPPEIQRLQTRLWTEFKSGR

Sequences:

>Translated_366_residues
MKRRVVGQVAALVLCATPWLTAAAKDTQLNVYNWSDYIAKDTIPNFTKQTGVQVRYDNYDSDDTLQAKLLTGNSGYDIVV
PTSNYAGKQIAAGIFAPLDKSKLPNLKYLDPSLMALVAGADPGNKFTVPWAYGTTGLGYNVTKAQQILGKNVPLDSWDVL
FKPENISKLKACGVSVLDAPDQMFAAALHYIGKDPMSTNPADYRAALEMMKKIRPYITQFNSSGYINDLVGGDVCFAYGW
SGDIVIAKHRAVEAKKAYKVEYYIPKGGAPVWFDVMAIPKDAKNKEAALEWINYIETPQVHAAITNAVYYPSANLEARKY
VDKDVANDPAVYPPPEVIKTLFLLKPLPPEIQRLQTRLWTEFKSGR
>Mature_366_residues
MKRRVVGQVAALVLCATPWLTAAAKDTQLNVYNWSDYIAKDTIPNFTKQTGVQVRYDNYDSDDTLQAKLLTGNSGYDIVV
PTSNYAGKQIAAGIFAPLDKSKLPNLKYLDPSLMALVAGADPGNKFTVPWAYGTTGLGYNVTKAQQILGKNVPLDSWDVL
FKPENISKLKACGVSVLDAPDQMFAAALHYIGKDPMSTNPADYRAALEMMKKIRPYITQFNSSGYINDLVGGDVCFAYGW
SGDIVIAKHRAVEAKKAYKVEYYIPKGGAPVWFDVMAIPKDAKNKEAALEWINYIETPQVHAAITNAVYYPSANLEARKY
VDKDVANDPAVYPPPEVIKTLFLLKPLPPEIQRLQTRLWTEFKSGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=367, Percent_Identity=49.3188010899183, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1787367, Length=319, Percent_Identity=35.1097178683386, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1787711, Length=297, Percent_Identity=23.2323232323232, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40502; Mature: 40502

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRVVGQVAALVLCATPWLTAAAKDTQLNVYNWSDYIAKDTIPNFTKQTGVQVRYDNYD
CCCHHHHHHHHHHHHHCCHHHHCCCCCEEEEEECHHHHHHCCCCCCHHHCCCEEEECCCC
SDDTLQAKLLTGNSGYDIVVPTSNYAGKQIAAGIFAPLDKSKLPNLKYLDPSLMALVAGA
CCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCEECCHHHEEEEECC
DPGNKFTVPWAYGTTGLGYNVTKAQQILGKNVPLDSWDVLFKPENISKLKACGVSVLDAP
CCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCCEEECCC
DQMFAAALHYIGKDPMSTNPADYRAALEMMKKIRPYITQFNSSGYINDLVGGDVCFAYGW
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECC
SGDIVIAKHRAVEAKKAYKVEYYIPKGGAPVWFDVMAIPKDAKNKEAALEWINYIETPQV
CCCEEEEECCHHCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCH
HAAITNAVYYPSANLEARKYVDKDVANDPAVYPPPEVIKTLFLLKPLPPEIQRLQTRLWT
HHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
EFKSGR
HHCCCC
>Mature Secondary Structure
MKRRVVGQVAALVLCATPWLTAAAKDTQLNVYNWSDYIAKDTIPNFTKQTGVQVRYDNYD
CCCHHHHHHHHHHHHHCCHHHHCCCCCEEEEEECHHHHHHCCCCCCHHHCCCEEEECCCC
SDDTLQAKLLTGNSGYDIVVPTSNYAGKQIAAGIFAPLDKSKLPNLKYLDPSLMALVAGA
CCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCEECCHHHEEEEECC
DPGNKFTVPWAYGTTGLGYNVTKAQQILGKNVPLDSWDVLFKPENISKLKACGVSVLDAP
CCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCCEEECCC
DQMFAAALHYIGKDPMSTNPADYRAALEMMKKIRPYITQFNSSGYINDLVGGDVCFAYGW
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECC
SGDIVIAKHRAVEAKKAYKVEYYIPKGGAPVWFDVMAIPKDAKNKEAALEWINYIETPQV
CCCEEEEECCHHCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCH
HAAITNAVYYPSANLEARKYVDKDVANDPAVYPPPEVIKTLFLLKPLPPEIQRLQTRLWT
HHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
EFKSGR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]