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The map label for this gene is yafC [C]

Identifier: 91783841

GI number: 91783841

Start: 2749801

End: 2750868

Strand: Reverse

Name: yafC [C]

Synonym: Bxe_A1971

Alternate gene names: 91783841

Gene position: 2750868-2749801 (Counterclockwise)

Preceding gene: 91783843

Following gene: 91783834

Centisome position: 56.19

GC content: 66.95

Gene sequence:

>1068_bases
ATGAACCAATTGCAGTCGATGCGCGTATTCGTCAAGGTGGCCGACCTCGGCAGTTTCGTCGGCGCGGCGGGCGCGCTGGA
TCTTTCCACCGCGGTCGTTACGCGCCACGTAGCCGATCTGGAAGCGCGCCTCGGCACGCGGCTGCTCAATCGCACCACGC
GCCGCCTGTCGCTGACCGAATCCGGCAGCACGTATCTGGAACGCGTGCGCCACATTCTCGACGACCTGGAAGGCGTGGAG
CAGATGGTGGTGGCGCGCAATCACGAGCCGGTCGGCACCTTGCGGATCGTCGCGCCCGTGGTGTTCGGGCTGCATAGTCT
CGCACCGGTGGTGCAGTCTTATGCCGCGCGGTATCCGGATGTCGTGCCCGACGTCACGCTCGCGGATCGGCATGTCGATC
TGGTGGAAGAGGGCTTCGATGTCGGCATTCTCGTGGCGCGGCACATGCGCGGCGCGAGCATCGTCACGCGGCGGTTGACC
ACGGGCTTTATGACGGTTTGCGCGACGCCTGCTTATCTGGCGCAGCATGGCACGCCGACGCGGCCCGAGCATCTGCTGGC
GCATCCGTGCCTGAGCCGGCCGGCCGAGCAGGGCGGCGAGGAGCGCGTGTTCGCCGGGCCGGACGGCGAAGTGCGCGTGC
GTCCCACGAACGCGATCGTGGCGAACAACGCGGAGATGCTCAGGCAATTCGCGCTGCTGGGCATGGGCGTGGCGATTCTG
CCGAGCTATCTGATTGGCCGCGATCTGGCGTCCGGGCGGCTCGTGCCGCTGCTTTCCAACTACCGTCTGCCGCAGATCGA
AATCACCATCGCCTATCCGAGCCGCCTGCATCTGCCGGCGAAGGTGCGCACTTTCATCGACCATCTGGTCGAGTATTTCC
AGCAGCCGGGTCAGCAACCGCGCGATCCGCGCATGATGACGCCGGTGCGTTCCGTGGCGGATAAGGTGGATAGAGTGGAG
ATGGCGAAGGGTGCGAAAAGCCTGCCGACGCCGGCGCTGTCCGCGGACGCGGCGCGCCCCGCGCATAAGGCGGCGCGCGC
GCGGATCGCCGCTTCGTCGCCGTTCTAG

Upstream 100 bases:

>100_bases
AATCGGAAGCGTTGGGCAAACCCGCGCGCGCCTTGTCGCCCGGGTCGACATAGGCGCGAAGGTTGCGCCGGCCCGGCTTT
ACCGCGGCAGGAGTCGTCCC

Downstream 100 bases:

>100_bases
TTCCGGCGCGCGGCGCGCGAAGAAAATCGCGCTGAGGAGTCGGGTGGCCGCGACGCGCCGCAAAAGCGCGCGTGGCTACG
ATGCCGGCACAAGCACACAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MNQLQSMRVFVKVADLGSFVGAAGALDLSTAVVTRHVADLEARLGTRLLNRTTRRLSLTESGSTYLERVRHILDDLEGVE
QMVVARNHEPVGTLRIVAPVVFGLHSLAPVVQSYAARYPDVVPDVTLADRHVDLVEEGFDVGILVARHMRGASIVTRRLT
TGFMTVCATPAYLAQHGTPTRPEHLLAHPCLSRPAEQGGEERVFAGPDGEVRVRPTNAIVANNAEMLRQFALLGMGVAIL
PSYLIGRDLASGRLVPLLSNYRLPQIEITIAYPSRLHLPAKVRTFIDHLVEYFQQPGQQPRDPRMMTPVRSVADKVDRVE
MAKGAKSLPTPALSADAARPAHKAARARIAASSPF

Sequences:

>Translated_355_residues
MNQLQSMRVFVKVADLGSFVGAAGALDLSTAVVTRHVADLEARLGTRLLNRTTRRLSLTESGSTYLERVRHILDDLEGVE
QMVVARNHEPVGTLRIVAPVVFGLHSLAPVVQSYAARYPDVVPDVTLADRHVDLVEEGFDVGILVARHMRGASIVTRRLT
TGFMTVCATPAYLAQHGTPTRPEHLLAHPCLSRPAEQGGEERVFAGPDGEVRVRPTNAIVANNAEMLRQFALLGMGVAIL
PSYLIGRDLASGRLVPLLSNYRLPQIEITIAYPSRLHLPAKVRTFIDHLVEYFQQPGQQPRDPRMMTPVRSVADKVDRVE
MAKGAKSLPTPALSADAARPAHKAARARIAASSPF
>Mature_355_residues
MNQLQSMRVFVKVADLGSFVGAAGALDLSTAVVTRHVADLEARLGTRLLNRTTRRLSLTESGSTYLERVRHILDDLEGVE
QMVVARNHEPVGTLRIVAPVVFGLHSLAPVVQSYAARYPDVVPDVTLADRHVDLVEEGFDVGILVARHMRGASIVTRRLT
TGFMTVCATPAYLAQHGTPTRPEHLLAHPCLSRPAEQGGEERVFAGPDGEVRVRPTNAIVANNAEMLRQFALLGMGVAIL
PSYLIGRDLASGRLVPLLSNYRLPQIEITIAYPSRLHLPAKVRTFIDHLVEYFQQPGQQPRDPRMMTPVRSVADKVDRVE
MAKGAKSLPTPALSADAARPAHKAARARIAASSPF

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=33, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=32.1070234113712, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=33.7837837837838, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=29.553264604811, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787128, Length=304, Percent_Identity=29.9342105263158, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1789440, Length=293, Percent_Identity=29.0102389078498, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=26.551724137931, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790011, Length=307, Percent_Identity=23.7785016286645, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1789173, Length=252, Percent_Identity=26.5873015873016, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788706, Length=263, Percent_Identity=25.8555133079848, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=312, Percent_Identity=25, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38724; Mature: 38724

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLQSMRVFVKVADLGSFVGAAGALDLSTAVVTRHVADLEARLGTRLLNRTTRRLSLTE
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
SGSTYLERVRHILDDLEGVEQMVVARNHEPVGTLRIVAPVVFGLHSLAPVVQSYAARYPD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVPDVTLADRHVDLVEEGFDVGILVARHMRGASIVTRRLTTGFMTVCATPAYLAQHGTPT
CCCCCEECHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
RPEHLLAHPCLSRPAEQGGEERVFAGPDGEVRVRPTNAIVANNAEMLRQFALLGMGVAIL
CHHHHHHHHHHCCHHHCCCCCEEEECCCCCEEEEECCEEEECCHHHHHHHHHHHCCHHHH
PSYLIGRDLASGRLVPLLSNYRLPQIEITIAYPSRLHLPAKVRTFIDHLVEYFQQPGQQP
HHHHHHHHCCCCCEEHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
RDPRMMTPVRSVADKVDRVEMAKGAKSLPTPALSADAARPAHKAARARIAASSPF
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNQLQSMRVFVKVADLGSFVGAAGALDLSTAVVTRHVADLEARLGTRLLNRTTRRLSLTE
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
SGSTYLERVRHILDDLEGVEQMVVARNHEPVGTLRIVAPVVFGLHSLAPVVQSYAARYPD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVPDVTLADRHVDLVEEGFDVGILVARHMRGASIVTRRLTTGFMTVCATPAYLAQHGTPT
CCCCCEECHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
RPEHLLAHPCLSRPAEQGGEERVFAGPDGEVRVRPTNAIVANNAEMLRQFALLGMGVAIL
CHHHHHHHHHHCCHHHCCCCCEEEECCCCCEEEEECCEEEECCHHHHHHHHHHHCCHHHH
PSYLIGRDLASGRLVPLLSNYRLPQIEITIAYPSRLHLPAKVRTFIDHLVEYFQQPGQQP
HHHHHHHHCCCCCEEHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
RDPRMMTPVRSVADKVDRVEMAKGAKSLPTPALSADAARPAHKAARARIAASSPF
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]