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The map label for this gene is purR [H]

Identifier: 91783812

GI number: 91783812

Start: 2718467

End: 2719507

Strand: Reverse

Name: purR [H]

Synonym: Bxe_A2000

Alternate gene names: 91783812

Gene position: 2719507-2718467 (Counterclockwise)

Preceding gene: 91783813

Following gene: 91783811

Centisome position: 55.55

GC content: 65.9

Gene sequence:

>1041_bases
ATGGCGACGATCAAGGATGTAGCGGCCGCGGCGGGCGTGTCGTTCACGACGGTATCCCACGTGGTGAACAACTCGCGGCC
GGTGTCGGCCGACGTGCGCGCGAAGGTCGAACACGCGATCCGTCAGCTTCACTATGTTCCGTCGGCGGTGGCCCGCTCGC
TGAAGGCGCGTTCGACGGCGACGATCGGGCTCGTCGTGCCGAACACCACGAACCCGTATTTCGCGGAACTGGCGCGCGGG
ATCGAAGACGGTTGCTCACGCAACGGCTATTGCGTGTTCTTCTGCAATTCCGACGACGATCCGGCCAAGCAGCGCAACTA
TCTTCGCGTGCTGCAGGAAAAGCGCATCGACGGCCTGATCGTCGCCTCCGCCGGCGACGACGCCGTGCTCGCGCAGACGC
TCGCCGACTCGCGCGAGCCGCTGGTCATTCTGGATCGCAACATCGAAGGGCTGAACGCGGATCTGGTGCAGATCGACCAC
GAGAAGGGCGCGTATTTCGCGACCCGGCACCTGCTCGAACTGGGGCACGTGCGGATCGGCTGCATAACGGGGCCGGTTCA
GACGGCGGTCAGCGCAATGCGCGTGCACGGTTTTATCCGTGCGATGACCGAGCGCGGCATCGAGATTCCGCCCGATGCGA
TCGTCGAAAGCGATTTTTCGGGCACCGGCGGCCACCGTGCGGCCGGCCAGTTGTTCGACACGGTCAAACCGTCGGCGATT
TTTGCCTGCAACGACATGATGGGCATCGGCGCGCTGCGCGCAGCCGCCGAACGCAACATCAGCGTGCCGCGTGATTGCTC
GATCATCGGTTTCGACGATATCGAACTGGGACGCTTCACTTATCCAGCGCTCTCGACCGTCGGGCAATCGGTGCGCGCGC
TCGGCGACATGGCCGCGCAGACGCTGATCGAGCGGATCGCCGGCACCTCGTCCGGCAGTCCGCGTCCGCCGGGGCGCCGG
CGCGTCGTTTCGCCGCGCCTGATCGTGCGCGAATCCACCGCGACCTGGGGCGGTGCGGCCAAACGCAATCTGGCGGCCTG
A

Upstream 100 bases:

>100_bases
GCGTAAGAATTCATGGGTTTGGTCAGTCTTCGAAGAAGACGTCCGGTGGGTCGCCAGGTGGCGTGGCCGGTGGGCAAAAC
AGGCAGGAGAGCAACAGGTT

Downstream 100 bases:

>100_bases
GGCGCCGCCGTGCGCGGACTTTCAGACGCGCATTCGCCCAGCGGCGGACCGCGGCTACTTGCGCTACGCATTCGTGCGTA
ACCGCTGCTGATAACCGGAA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MATIKDVAAAAGVSFTTVSHVVNNSRPVSADVRAKVEHAIRQLHYVPSAVARSLKARSTATIGLVVPNTTNPYFAELARG
IEDGCSRNGYCVFFCNSDDDPAKQRNYLRVLQEKRIDGLIVASAGDDAVLAQTLADSREPLVILDRNIEGLNADLVQIDH
EKGAYFATRHLLELGHVRIGCITGPVQTAVSAMRVHGFIRAMTERGIEIPPDAIVESDFSGTGGHRAAGQLFDTVKPSAI
FACNDMMGIGALRAAAERNISVPRDCSIIGFDDIELGRFTYPALSTVGQSVRALGDMAAQTLIERIAGTSSGSPRPPGRR
RVVSPRLIVRESTATWGGAAKRNLAA

Sequences:

>Translated_346_residues
MATIKDVAAAAGVSFTTVSHVVNNSRPVSADVRAKVEHAIRQLHYVPSAVARSLKARSTATIGLVVPNTTNPYFAELARG
IEDGCSRNGYCVFFCNSDDDPAKQRNYLRVLQEKRIDGLIVASAGDDAVLAQTLADSREPLVILDRNIEGLNADLVQIDH
EKGAYFATRHLLELGHVRIGCITGPVQTAVSAMRVHGFIRAMTERGIEIPPDAIVESDFSGTGGHRAAGQLFDTVKPSAI
FACNDMMGIGALRAAAERNISVPRDCSIIGFDDIELGRFTYPALSTVGQSVRALGDMAAQTLIERIAGTSSGSPRPPGRR
RVVSPRLIVRESTATWGGAAKRNLAA
>Mature_345_residues
ATIKDVAAAAGVSFTTVSHVVNNSRPVSADVRAKVEHAIRQLHYVPSAVARSLKARSTATIGLVVPNTTNPYFAELARGI
EDGCSRNGYCVFFCNSDDDPAKQRNYLRVLQEKRIDGLIVASAGDDAVLAQTLADSREPLVILDRNIEGLNADLVQIDHE
KGAYFATRHLLELGHVRIGCITGPVQTAVSAMRVHGFIRAMTERGIEIPPDAIVESDFSGTGGHRAAGQLFDTVKPSAIF
ACNDMMGIGALRAAAERNISVPRDCSIIGFDDIELGRFTYPALSTVGQSVRALGDMAAQTLIERIAGTSSGSPRPPGRRR
VVSPRLIVRESTATWGGAAKRNLAA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=341, Percent_Identity=39.5894428152493, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1787948, Length=337, Percent_Identity=40.6528189910979, Blast_Score=240, Evalue=8e-65,
Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=34.0298507462687, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1788474, Length=346, Percent_Identity=36.4161849710983, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789202, Length=338, Percent_Identity=33.7278106508876, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789068, Length=341, Percent_Identity=30.4985337243402, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1786540, Length=314, Percent_Identity=30.8917197452229, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=29.7213622291022, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI48994940, Length=309, Percent_Identity=26.8608414239482, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=25.6329113924051, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787906, Length=345, Percent_Identity=26.0869565217391, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786268, Length=331, Percent_Identity=27.7945619335347, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789456, Length=319, Percent_Identity=26.6457680250784, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790689, Length=312, Percent_Identity=24.3589743589744, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36974; Mature: 36842

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAAAAGVSFTTVSHVVNNSRPVSADVRAKVEHAIRQLHYVPSAVARSLKARSTA
CCCHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TIGLVVPNTTNPYFAELARGIEDGCSRNGYCVFFCNSDDDPAKQRNYLRVLQEKRIDGLI
EEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
VASAGDDAVLAQTLADSREPLVILDRNIEGLNADLVQIDHEKGAYFATRHLLELGHVRIG
EECCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEE
CITGPVQTAVSAMRVHGFIRAMTERGIEIPPDAIVESDFSGTGGHRAAGQLFDTVKPSAI
EECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCEE
FACNDMMGIGALRAAAERNISVPRDCSIIGFDDIELGRFTYPALSTVGQSVRALGDMAAQ
EEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLIERIAGTSSGSPRPPGRRRVVSPRLIVRESTATWGGAAKRNLAA
HHHHHHHCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVAAAAGVSFTTVSHVVNNSRPVSADVRAKVEHAIRQLHYVPSAVARSLKARSTA
CCHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TIGLVVPNTTNPYFAELARGIEDGCSRNGYCVFFCNSDDDPAKQRNYLRVLQEKRIDGLI
EEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
VASAGDDAVLAQTLADSREPLVILDRNIEGLNADLVQIDHEKGAYFATRHLLELGHVRIG
EECCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEE
CITGPVQTAVSAMRVHGFIRAMTERGIEIPPDAIVESDFSGTGGHRAAGQLFDTVKPSAI
EECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCEE
FACNDMMGIGALRAAAERNISVPRDCSIIGFDDIELGRFTYPALSTVGQSVRALGDMAAQ
EEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLIERIAGTSSGSPRPPGRRRVVSPRLIVRESTATWGGAAKRNLAA
HHHHHHHCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA