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The map label for this gene is hsrA [H]

Identifier: 91783781

GI number: 91783781

Start: 2681870

End: 2683267

Strand: Direct

Name: hsrA [H]

Synonym: Bxe_A2031

Alternate gene names: 91783781

Gene position: 2681870-2683267 (Clockwise)

Preceding gene: 91783780

Following gene: 91783787

Centisome position: 54.78

GC content: 65.88

Gene sequence:

>1398_bases
ATGACCGTCGCCCAGAAAGCCCTGATTGCGCCCCTTATCGTCGCGTGCGCAATGTTCATGGAAAGCGTGGATGCGAACGT
GATCGTTACCGCGCTGCCGGCAATGGCGCGCGACTTCGGCCGCGATCCGGTCACGCTGAAAATCGCGGTGACGAGCTACG
TGCTCGGGCTCGGCGTGTTCATCCCCGTGTGCGGCTGGCTCGCCGACCGGTTCGGCGCGCGCACGATCTTCCGCACGGCG
ATCGGCATTTTCGTGACGGGTTCGCTGCTGTGCGCCGCGTCGAACTCTCTCGCCACGTTCACGCTCGCGCGCTTCGTGCA
GGGTGTCGGCGGCGCGATGATGGTGCCGGTCGGGCGGATCATCATTTTTCGGGTGGTGCACAAGGCCGACTTCATCCGCG
CGATGAACTACCTGAGCGTCCCCGCGATGCTCGGCCCGGCGGCGGGGCCGCTGCTCGGCGGTTTCATCACCACCTATCTG
CACTGGCGGCTGATTTTTTTCATCAACGTGCCGATCGGCATTCTGGGCATCTATCTGACCAACCGGCACATCGCCAACAC
GCGCGAACCCGATCCCGGCCCGCTCGACTGGATCGGCTTTTTCCTGTCGGCCGCGGGCGCGGTGTTGCTGCTGCTCGGGT
TGTCACTGGTCGGCGGCGAACTGATTTCGAATCGCGACGCGTTCGGCATGTGCGCGAGCGGCGCCGTTCTGCTCGCGGCC
TACGTGGCCTACGCGCAGCGCGTTCCGCTGCCGCTGCTGGACCTGCGCTTTTTCAAGGTGCCCACGTTCCAGGCGAGCGT
GCTCGGCGGTTCGCTGTTCCGTATCGGGCTCGGCGCGTTGCCGTTCCTGTTGCCGCTGATGCTGCAGGAAGGCCACGGCA
TGAGCGCGTTCGAATCCGGCCTGATCACCTGCGCGTCCGCATTCGGCGGCATGTTCATGCGCACGGTCGCTTCGAGCGTG
CTGCATCGCTTCGGCTTCCGCTCGGTGCTGGTGGTCAACGCCGCGCTGTCGGGGCTTTCTATCGCGGCTTGCGGCCTGTT
CTTTCCCGGCACGCCGACCTGGATCATCTGGGCGATCGTGCTGCTCGGCGGGTTCTTTCCCGCGTTGCAGTTCACCAGTC
TGAACTCGCTGACTTACGCGGAGATCGCCAGCCGCGACGTGGGCCGCGCCACCAGTCTCGGCAGTGTCGTGCAGCAGATG
TCGCTCGGGCTGGGCGTGACGATCGGCGGTATCGTGCTGCAGATTTCGCGCGTGCTGCACGGACATCCGGCCCTCATGTG
GTCGGATTTCTGGCCGGCGTTTCTGGTGGTCGGCCTGTGCTCGTTCGCGTCGATTCCGGTCACGCTGCGCATGCCGCATC
GCGCGGGCGAGGAAATCTCGCGCGGCGGCCGCGGCTGA

Upstream 100 bases:

>100_bases
TTAAAAAGGGCGGCATCGCAACGTTGTCTTTTAACAACCCGCACCGTACAGGTGCAATAATGCCGCTTTATCTGCGAACG
CCCGCCGCAGCCCCGCCTTC

Downstream 100 bases:

>100_bases
GCGGGCGCACCGGTCGCTGCCGTCGCCGTCACTGTCGGCCGGCTTCAGCGCTTCTTGCGGATCACGCTCACTTCGCCATT
GCGTTCGAGAATGGCCGCGG

Product: major facilitator superfamily multidrug efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTVAQKALIAPLIVACAMFMESVDANVIVTALPAMARDFGRDPVTLKIAVTSYVLGLGVFIPVCGWLADRFGARTIFRTA
IGIFVTGSLLCAASNSLATFTLARFVQGVGGAMMVPVGRIIIFRVVHKADFIRAMNYLSVPAMLGPAAGPLLGGFITTYL
HWRLIFFINVPIGILGIYLTNRHIANTREPDPGPLDWIGFFLSAAGAVLLLLGLSLVGGELISNRDAFGMCASGAVLLAA
YVAYAQRVPLPLLDLRFFKVPTFQASVLGGSLFRIGLGALPFLLPLMLQEGHGMSAFESGLITCASAFGGMFMRTVASSV
LHRFGFRSVLVVNAALSGLSIAACGLFFPGTPTWIIWAIVLLGGFFPALQFTSLNSLTYAEIASRDVGRATSLGSVVQQM
SLGLGVTIGGIVLQISRVLHGHPALMWSDFWPAFLVVGLCSFASIPVTLRMPHRAGEEISRGGRG

Sequences:

>Translated_465_residues
MTVAQKALIAPLIVACAMFMESVDANVIVTALPAMARDFGRDPVTLKIAVTSYVLGLGVFIPVCGWLADRFGARTIFRTA
IGIFVTGSLLCAASNSLATFTLARFVQGVGGAMMVPVGRIIIFRVVHKADFIRAMNYLSVPAMLGPAAGPLLGGFITTYL
HWRLIFFINVPIGILGIYLTNRHIANTREPDPGPLDWIGFFLSAAGAVLLLLGLSLVGGELISNRDAFGMCASGAVLLAA
YVAYAQRVPLPLLDLRFFKVPTFQASVLGGSLFRIGLGALPFLLPLMLQEGHGMSAFESGLITCASAFGGMFMRTVASSV
LHRFGFRSVLVVNAALSGLSIAACGLFFPGTPTWIIWAIVLLGGFFPALQFTSLNSLTYAEIASRDVGRATSLGSVVQQM
SLGLGVTIGGIVLQISRVLHGHPALMWSDFWPAFLVVGLCSFASIPVTLRMPHRAGEEISRGGRG
>Mature_464_residues
TVAQKALIAPLIVACAMFMESVDANVIVTALPAMARDFGRDPVTLKIAVTSYVLGLGVFIPVCGWLADRFGARTIFRTAI
GIFVTGSLLCAASNSLATFTLARFVQGVGGAMMVPVGRIIIFRVVHKADFIRAMNYLSVPAMLGPAAGPLLGGFITTYLH
WRLIFFINVPIGILGIYLTNRHIANTREPDPGPLDWIGFFLSAAGAVLLLLGLSLVGGELISNRDAFGMCASGAVLLAAY
VAYAQRVPLPLLDLRFFKVPTFQASVLGGSLFRIGLGALPFLLPLMLQEGHGMSAFESGLITCASAFGGMFMRTVASSVL
HRFGFRSVLVVNAALSGLSIAACGLFFPGTPTWIIWAIVLLGGFFPALQFTSLNSLTYAEIASRDVGRATSLGSVVQQMS
LGLGVTIGGIVLQISRVLHGHPALMWSDFWPAFLVVGLCSFASIPVTLRMPHRAGEEISRGGRG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=454, Percent_Identity=35.2422907488987, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788392, Length=407, Percent_Identity=38.0835380835381, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI87081983, Length=402, Percent_Identity=27.363184079602, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1789042, Length=434, Percent_Identity=25.1152073732719, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788710, Length=408, Percent_Identity=25.4901960784314, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325455, Length=258, Percent_Identity=29.0697674418605, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=235, Percent_Identity=28.0851063829787, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49343; Mature: 49212

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAQKALIAPLIVACAMFMESVDANVIVTALPAMARDFGRDPVTLKIAVTSYVLGLGVF
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
IPVCGWLADRFGARTIFRTAIGIFVTGSLLCAASNSLATFTLARFVQGVGGAMMVPVGRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
IIFRVVHKADFIRAMNYLSVPAMLGPAAGPLLGGFITTYLHWRLIFFINVPIGILGIYLT
HHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
NRHIANTREPDPGPLDWIGFFLSAAGAVLLLLGLSLVGGELISNRDAFGMCASGAVLLAA
CCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHH
YVAYAQRVPLPLLDLRFFKVPTFQASVLGGSLFRIGLGALPFLLPLMLQEGHGMSAFESG
HHHHHHCCCCCHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LITCASAFGGMFMRTVASSVLHRFGFRSVLVVNAALSGLSIAACGLFFPGTPTWIIWAIV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHH
LLGGFFPALQFTSLNSLTYAEIASRDVGRATSLGSVVQQMSLGLGVTIGGIVLQISRVLH
HHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GHPALMWSDFWPAFLVVGLCSFASIPVTLRMPHRAGEEISRGGRG
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHCCCCC
>Mature Secondary Structure 
TVAQKALIAPLIVACAMFMESVDANVIVTALPAMARDFGRDPVTLKIAVTSYVLGLGVF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH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CCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]