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The map label for this gene is insF1 [C]

Identifier: 91783727

GI number: 91783727

Start: 2622274

End: 2623164

Strand: Reverse

Name: insF1 [C]

Synonym: Bxe_A2087

Alternate gene names: 91783727

Gene position: 2623164-2622274 (Counterclockwise)

Preceding gene: 91783728

Following gene: 91783717

Centisome position: 53.58

GC content: 61.95

Gene sequence:

>891_bases
ATGAAGTACGCCTGGATCGAGCTTCACAGCCGACAATGGCCGGTGTCCCTGACCTGCCAGGTGCTGGGTGTCAGCCCCAG
CGGTTACCACGCGCGCAAGGCGCGGGATGTCGATACTGACCGAGCGCGCCGACGCATCAGCAACGACGCTCTGCTGGTGC
ACATCAAGGCCGTGCACGCTGAATCCAAAGGCGAGTACGGCTGGCCGCGCGTGTGGAAGAAGTTGCTGGCCCAGGGCATT
CGCGTCAGCAAGGATCGTGTCCAACAGCTCATGAAGCTGCACGGCATCAGGGCGAAGACCAAACGCCGGTTCAAGGTCAC
GACCGACAGCAACCACAGCCTGCCGGTCGCGCCGGACCTGCTGCAACGAGACTTCTCTCCCGCGCGTCCAGATCAGGTCT
GGACTACGGACATCACGTACATCTGGACGGACGAGGGTTGGCTGTTTCTGACCGTCATCCTCGACCTGTTCAGCCGTCAG
GTGGTGGGCTGGTCGATGCAGCCGCACATGCGCACGGAGCTGGTGTCTGACGCGCTGCGTATGGCGTGGTTTCGCCGCCG
GCCGGAAGCGGGCCTGATCGTCCATAGCGACCGCGGTAGCCAGTATTGCAGTCGTGACTTCCAGGACCTGCTTAAGGGCT
ACGGCATGCGCAGCTCGATGAGCCGTCGAGGAAATTGTTGGGACAACGCGCCGACCGAGAGCTTGTGGGGATCGCTCAAG
CGAGCACGCATCCTTGGCCAGCGCTTTGCAACGCGTCGGGAAGCGATGGACGAGGTAATCGACTGGTTGAGCTTCTACAA
TCATTCGCGCTTGCACTCGACGTTGGGCTACGTCAGTCCGATGCAGTTCGAGCGGGACTGGTACGCTGCCCAGAACCAAC
GGGTGGCATAA

Upstream 100 bases:

>100_bases
AACAGATGGAGATTGCCCGGCTGCGCGCGGAGTTGGCACGCGTGAAAATGGAGCGCGACATATTGGAAAAAGCCACGGCG
TACTTTGCAAAGGTGTCGGC

Downstream 100 bases:

>100_bases
TCTCACTCAGCTAAGGGATCCGAAATTCGCGGGCAACGTCAACTTCTTCATGCTTGAATTTCCCGAGTGGCCTTTTTGAC
CCGCTATTGCTTTGACGTTA

Product: putative IS3 family transposase orfB

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKYAWIELHSRQWPVSLTCQVLGVSPSGYHARKARDVDTDRARRRISNDALLVHIKAVHAESKGEYGWPRVWKKLLAQGI
RVSKDRVQQLMKLHGIRAKTKRRFKVTTDSNHSLPVAPDLLQRDFSPARPDQVWTTDITYIWTDEGWLFLTVILDLFSRQ
VVGWSMQPHMRTELVSDALRMAWFRRRPEAGLIVHSDRGSQYCSRDFQDLLKGYGMRSSMSRRGNCWDNAPTESLWGSLK
RARILGQRFATRREAMDEVIDWLSFYNHSRLHSTLGYVSPMQFERDWYAAQNQRVA

Sequences:

>Translated_296_residues
MKYAWIELHSRQWPVSLTCQVLGVSPSGYHARKARDVDTDRARRRISNDALLVHIKAVHAESKGEYGWPRVWKKLLAQGI
RVSKDRVQQLMKLHGIRAKTKRRFKVTTDSNHSLPVAPDLLQRDFSPARPDQVWTTDITYIWTDEGWLFLTVILDLFSRQ
VVGWSMQPHMRTELVSDALRMAWFRRRPEAGLIVHSDRGSQYCSRDFQDLLKGYGMRSSMSRRGNCWDNAPTESLWGSLK
RARILGQRFATRREAMDEVIDWLSFYNHSRLHSTLGYVSPMQFERDWYAAQNQRVA
>Mature_296_residues
MKYAWIELHSRQWPVSLTCQVLGVSPSGYHARKARDVDTDRARRRISNDALLVHIKAVHAESKGEYGWPRVWKKLLAQGI
RVSKDRVQQLMKLHGIRAKTKRRFKVTTDSNHSLPVAPDLLQRDFSPARPDQVWTTDITYIWTDEGWLFLTVILDLFSRQ
VVGWSMQPHMRTELVSDALRMAWFRRRPEAGLIVHSDRGSQYCSRDFQDLLKGYGMRSSMSRRGNCWDNAPTESLWGSLK
RARILGQRFATRREAMDEVIDWLSFYNHSRLHSTLGYVSPMQFERDWYAAQNQRVA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=256, Percent_Identity=32.8125, Blast_Score=145, Evalue=3e-35,
Organism=Escherichia coli, GI1788405, Length=288, Percent_Identity=42.0138888888889, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1787263, Length=288, Percent_Identity=42.0138888888889, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1786753, Length=288, Percent_Identity=42.0138888888889, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1786570, Length=288, Percent_Identity=42.0138888888889, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1786490, Length=288, Percent_Identity=42.0138888888889, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1789981, Length=235, Percent_Identity=31.9148936170213, Blast_Score=148, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 34619; Mature: 34619

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYAWIELHSRQWPVSLTCQVLGVSPSGYHARKARDVDTDRARRRISNDALLVHIKAVHA
CCEEEEEECCCCCCEEEEEEEEECCCCCCCHHHCCCCCHHHHHHHHCCCEEEEEEEEEEC
ESKGEYGWPRVWKKLLAQGIRVSKDRVQQLMKLHGIRAKTKRRFKVTTDSNHSLPVAPDL
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHCCCEEEEECCCCCCCCCCHHH
LQRDFSPARPDQVWTTDITYIWTDEGWLFLTVILDLFSRQVVGWSMQPHMRTELVSDALR
HHCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MAWFRRRPEAGLIVHSDRGSQYCSRDFQDLLKGYGMRSSMSRRGNCWDNAPTESLWGSLK
HHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHH
RARILGQRFATRREAMDEVIDWLSFYNHSRLHSTLGYVSPMQFERDWYAAQNQRVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKYAWIELHSRQWPVSLTCQVLGVSPSGYHARKARDVDTDRARRRISNDALLVHIKAVHA
CCEEEEEECCCCCCEEEEEEEEECCCCCCCHHHCCCCCHHHHHHHHCCCEEEEEEEEEEC
ESKGEYGWPRVWKKLLAQGIRVSKDRVQQLMKLHGIRAKTKRRFKVTTDSNHSLPVAPDL
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHCCCEEEEECCCCCCCCCCHHH
LQRDFSPARPDQVWTTDITYIWTDEGWLFLTVILDLFSRQVVGWSMQPHMRTELVSDALR
HHCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MAWFRRRPEAGLIVHSDRGSQYCSRDFQDLLKGYGMRSSMSRRGNCWDNAPTESLWGSLK
HHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHH
RARILGQRFATRREAMDEVIDWLSFYNHSRLHSTLGYVSPMQFERDWYAAQNQRVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]