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The map label for this gene is shiA [H]

Identifier: 91783711

GI number: 91783711

Start: 2605278

End: 2606594

Strand: Direct

Name: shiA [H]

Synonym: Bxe_A2103

Alternate gene names: 91783711

Gene position: 2605278-2606594 (Clockwise)

Preceding gene: 91783710

Following gene: 91783712

Centisome position: 53.21

GC content: 65.07

Gene sequence:

>1317_bases
ATGCCAAACGACACGTTCTCTCCCACGGCCGGATCGCCCCCCGCATCCGCCGCACCGGCTGCCTCGCGGACCTTGCGGCG
CATCATCGTCGCCTCCGTCCTCGGCAATGCGCTGGAGTGGTACGACTTTTTTCTGTACGGCACGGCCGCCGCACTGATCT
TCGGACCGCTGTTCTTCCCGTTGCACGGCGACCCGCTGCTGGGCACCCTTGCCGCGTTCGCCGGCTTCGCGATCGGCTTT
CTCGCGCGGCCGCTGGGCGGCATCGTGTTCGGCCATATCGGCGACCGCCACGGCCGCAAAGGCGCGCTCGTCGTCACGCT
GATGATGATGGGCGTGGCGACCTTCGGCATCGGCCTGCTGCCCACGTTCTCGCAGATCGGCTTTCTCGCGCCCACGGCGC
TCGTGTGTCTGCGCGTCGTGCAGGGCATCGCGTCGGGCGGCGAATGGGGCGGCGGCGTACTGCTGATCAGCGAAAGCGCA
CCGGCCAACCGGCGCGGCTACTACGCGGCGTTCAGTCAGTTGGGCGTCGCGGGCGGCTTCGTGCTGTCGGCGGCCGCGTT
CTATCTGGTGCAGCGCCTGCCGCTGGAAAGCTTCATGTCCTGGGGCTGGCGCGTGCCGTTTCTGGCCAGCGTGCTGATTT
TCGGCGTGGGCGTGTACATTCGCCGGCGCTTGCCCGAAAGCCGCGATTTCACCGCCGCCAAACCAAAGCACATGCCCGTG
CTGACGGTGATACGCAATCATCCGAAGCAGGTATTACAGGCTATGGGGCTGCGCGTCGCCGAAAACGGCGGGACGTATAT
CTTTCTTTCGTTCGTGCTGGTGTACGGCAAGACGATCGGCGTGCCCGTTTCGGTGATGCTCGGCGGCGTCATGATCGCCA
TGCTGCTCGAACTCGTCACAATCGTGTTGTGGGGGCGTTTGTCGGACCTGATCGGACGGCGGCCGGTGTATATGATCGGC
GCGCTCGGACTGGTGGTGATCGCGTTTCCCGCGTTCTGGCTGATCGACACGCACCAGCCGATGCTCGTCTTCCTTTCTCT
CGCGCTAGGCTTGCCGTTCTGCCACGCCGCGATGATCGGCACGCAGCCCGCGTTGATGGGCGAACTGTTTCCCACGGAAG
TGCGCTACTCGGGCATGGCGCTCGGGCATGAAATTGCCTCGGTATTTTCCGGCGGGCTCGCACCGCTCGTCGCCACCGCC
CTGTATGCGAAAGTGCGTGCGCCGTGGCCAGTGGCGCTGATGCTGATCGCTTTCGGGCTGATTACCGCGCTTACGCTCGT
GACCATTCCTCGCAACACCAGCGCCACGCATGATTGA

Upstream 100 bases:

>100_bases
CGCGCATCGTGCAGAAGGTGATCCGCCTGATCGATTCGACCGATGCGTTGAGCGCGATCGGCTTCCTGCTCTGAACCCCA
AAAAACCAGCGAGCACGACG

Downstream 100 bases:

>100_bases
GTTGTGATCAACCGGCCGGGGCGCCTCGGCGGTCGGGTGCTCGTAACCAGCACGCGTTTCCACGCGAAGGCGGCCCGGAC
AGCGGCGGTGGCTTGGTATT

Product: major facilitator superfamily metabolite/H(+) symporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPNDTFSPTAGSPPASAAPAASRTLRRIIVASVLGNALEWYDFFLYGTAAALIFGPLFFPLHGDPLLGTLAAFAGFAIGF
LARPLGGIVFGHIGDRHGRKGALVVTLMMMGVATFGIGLLPTFSQIGFLAPTALVCLRVVQGIASGGEWGGGVLLISESA
PANRRGYYAAFSQLGVAGGFVLSAAAFYLVQRLPLESFMSWGWRVPFLASVLIFGVGVYIRRRLPESRDFTAAKPKHMPV
LTVIRNHPKQVLQAMGLRVAENGGTYIFLSFVLVYGKTIGVPVSVMLGGVMIAMLLELVTIVLWGRLSDLIGRRPVYMIG
ALGLVVIAFPAFWLIDTHQPMLVFLSLALGLPFCHAAMIGTQPALMGELFPTEVRYSGMALGHEIASVFSGGLAPLVATA
LYAKVRAPWPVALMLIAFGLITALTLVTIPRNTSATHD

Sequences:

>Translated_438_residues
MPNDTFSPTAGSPPASAAPAASRTLRRIIVASVLGNALEWYDFFLYGTAAALIFGPLFFPLHGDPLLGTLAAFAGFAIGF
LARPLGGIVFGHIGDRHGRKGALVVTLMMMGVATFGIGLLPTFSQIGFLAPTALVCLRVVQGIASGGEWGGGVLLISESA
PANRRGYYAAFSQLGVAGGFVLSAAAFYLVQRLPLESFMSWGWRVPFLASVLIFGVGVYIRRRLPESRDFTAAKPKHMPV
LTVIRNHPKQVLQAMGLRVAENGGTYIFLSFVLVYGKTIGVPVSVMLGGVMIAMLLELVTIVLWGRLSDLIGRRPVYMIG
ALGLVVIAFPAFWLIDTHQPMLVFLSLALGLPFCHAAMIGTQPALMGELFPTEVRYSGMALGHEIASVFSGGLAPLVATA
LYAKVRAPWPVALMLIAFGLITALTLVTIPRNTSATHD
>Mature_437_residues
PNDTFSPTAGSPPASAAPAASRTLRRIIVASVLGNALEWYDFFLYGTAAALIFGPLFFPLHGDPLLGTLAAFAGFAIGFL
ARPLGGIVFGHIGDRHGRKGALVVTLMMMGVATFGIGLLPTFSQIGFLAPTALVCLRVVQGIASGGEWGGGVLLISESAP
ANRRGYYAAFSQLGVAGGFVLSAAAFYLVQRLPLESFMSWGWRVPFLASVLIFGVGVYIRRRLPESRDFTAAKPKHMPVL
TVIRNHPKQVLQAMGLRVAENGGTYIFLSFVLVYGKTIGVPVSVMLGGVMIAMLLELVTIVLWGRLSDLIGRRPVYMIGA
LGLVVIAFPAFWLIDTHQPMLVFLSLALGLPFCHAAMIGTQPALMGELFPTEVRYSGMALGHEIASVFSGGLAPLVATAL
YAKVRAPWPVALMLIAFGLITALTLVTIPRNTSATHD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=439, Percent_Identity=39.6355353075171, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1790550, Length=430, Percent_Identity=34.6511627906977, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1789941, Length=395, Percent_Identity=38.9873417721519, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788942, Length=418, Percent_Identity=30.8612440191388, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87082404, Length=377, Percent_Identity=25.7294429708223, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082231, Length=223, Percent_Identity=28.2511210762332, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46570; Mature: 46439

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PNDTFSPTAGSPPASAAPAASRTLRRIIVASVLGNALEWYDFFLYGTAAALIFGPLFFP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LHGDPLLGTLAAFAGFAIGFLARPLGGIVFGHIGDRHGRKGALVVTLMMMGVATFGIGLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
PTFSQIGFLAPTALVCLRVVQGIASGGEWGGGVLLISESAPANRRGYYAAFSQLGVAGGF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
VLSAAAFYLVQRLPLESFMSWGWRVPFLASVLIFGVGVYIRRRLPESRDFTAAKPKHMPV
HHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
LTVIRNHPKQVLQAMGLRVAENGGTYIFLSFVLVYGKTIGVPVSVMLGGVMIAMLLELVT
HHHHHCCHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IVLWGRLSDLIGRRPVYMIGALGLVVIAFPAFWLIDTHQPMLVFLSLALGLPFCHAAMIG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCHHHHHHHHC
TQPALMGELFPTEVRYSGMALGHEIASVFSGGLAPLVATALYAKVRAPWPVALMLIAFGL
CCHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ITALTLVTIPRNTSATHD
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]