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The map label for this gene is holB [H]

Identifier: 91783553

GI number: 91783553

Start: 2414790

End: 2415824

Strand: Direct

Name: holB [H]

Synonym: Bxe_A2262

Alternate gene names: 91783553

Gene position: 2414790-2415824 (Clockwise)

Preceding gene: 91783552

Following gene: 91783554

Centisome position: 49.32

GC content: 67.92

Gene sequence:

>1035_bases
ATGATTTATCCGTGGCAAGCCGATGACTGGAACCGCCTGCAGCAGCTGCGCGGCCACTGGCCTCACGCCTTGTTGCTGCA
CGGACAGGCGGGCATCGGCAAGCTGCGCTTCGCGCAGCACCTCGCGCAAGGCCTGCTGTGCGAGGCCCAGCAGGCCAACG
GCGAGCCGTGCGGCGCGTGCGTGGCCTGCAACTGGTTCGCGCAAGGCAATCACCCGGATTACCGGATCATCGTGCCCGAG
GCGCTGGCCGCCGAAGCCGGCCTCAACAATGCCGCGGACGAAAAAGCCGACAAGGCCGACGCCGACGAAGGCAAAAAGAC
CCGCACGCCCAGCAAGGAAATCAAGATCGAGCAGATTCGCGGCTTGCTCGACTTCGTCGGCGTGGGTTCGCACCGCGGCG
GCGCGCGCGTGGTCGTGCTGTATCCGGCGGAGGCGCTGAATATCGCCGCGGCCAACGCGCTTCTGAAGACGCTCGAAGAA
CCGCCGGCGGGCGTCGTGTTCCTGATGGTGTCGGCGCGTATCGACCGTCTTCTGCCAACCATCATCAGCCGCTGCCGCCA
ATGGCCGATGACCATGCCCGCGCCGGACGTGGCAACCCGGTGGCTTGCCGCGCAAGGCGTGGCCGAAGCGTCCGCGCTGC
TCGCCGAAGCCGGCGGCGCGCCGCTCACGGCGCTCGCGCTCGCCAGCGACGAAAATCGCGCGCTGCGCGACTGGACGCTG
AAACAGCTCGCCGCCGGCCCCGACTGCGACGCCTTCGCCTGCGGGGAGGCGCTGCAGAAGCTGCCCGTACCGCTGGTGCT
AGGCTGGCTGCAACGCTGGATGTACGATCTGCTGGCGCAGCGCACGGCGGGCGCGCCGCGTTATTTTCCGCAGGCGTCCG
CGGCGTTGACGCGCTGTGCGTCGCAAGCCGACGCTAGCGCGTTCGCGCGTTTCATGCGCACCGTGACGCGCCAGCGCGCC
GTCGAAAACCATCCGCTGAATGCGCGGCTGGTGTTCGAGGAACTGTTTATCGGCTACCGCGATCTGTTCGCTTAA

Upstream 100 bases:

>100_bases
CTTTGATTTTAAAAGAATATAATTCTGGTTTATAACGGTTTGAAGAGTTCTTGCTATATCTCGGATAACGTCTTCAACCG
ATTGATTTTAAAGAGAAACG

Downstream 100 bases:

>100_bases
ACCGCCTTAAGCTGCTTTAGGCCGCATCTACGGGGCACGCGGCGGCGGCCGGCGCCGGGTTCATCCTCCTGTTCGTCCTG
TATTGGCCTTCCATCTCACG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MIYPWQADDWNRLQQLRGHWPHALLLHGQAGIGKLRFAQHLAQGLLCEAQQANGEPCGACVACNWFAQGNHPDYRIIVPE
ALAAEAGLNNAADEKADKADADEGKKTRTPSKEIKIEQIRGLLDFVGVGSHRGGARVVVLYPAEALNIAAANALLKTLEE
PPAGVVFLMVSARIDRLLPTIISRCRQWPMTMPAPDVATRWLAAQGVAEASALLAEAGGAPLTALALASDENRALRDWTL
KQLAAGPDCDAFACGEALQKLPVPLVLGWLQRWMYDLLAQRTAGAPRYFPQASAALTRCASQADASAFARFMRTVTRQRA
VENHPLNARLVFEELFIGYRDLFA

Sequences:

>Translated_344_residues
MIYPWQADDWNRLQQLRGHWPHALLLHGQAGIGKLRFAQHLAQGLLCEAQQANGEPCGACVACNWFAQGNHPDYRIIVPE
ALAAEAGLNNAADEKADKADADEGKKTRTPSKEIKIEQIRGLLDFVGVGSHRGGARVVVLYPAEALNIAAANALLKTLEE
PPAGVVFLMVSARIDRLLPTIISRCRQWPMTMPAPDVATRWLAAQGVAEASALLAEAGGAPLTALALASDENRALRDWTL
KQLAAGPDCDAFACGEALQKLPVPLVLGWLQRWMYDLLAQRTAGAPRYFPQASAALTRCASQADASAFARFMRTVTRQRA
VENHPLNARLVFEELFIGYRDLFA
>Mature_344_residues
MIYPWQADDWNRLQQLRGHWPHALLLHGQAGIGKLRFAQHLAQGLLCEAQQANGEPCGACVACNWFAQGNHPDYRIIVPE
ALAAEAGLNNAADEKADKADADEGKKTRTPSKEIKIEQIRGLLDFVGVGSHRGGARVVVLYPAEALNIAAANALLKTLEE
PPAGVVFLMVSARIDRLLPTIISRCRQWPMTMPAPDVATRWLAAQGVAEASALLAEAGGAPLTALALASDENRALRDWTL
KQLAAGPDCDAFACGEALQKLPVPLVLGWLQRWMYDLLAQRTAGAPRYFPQASAALTRCASQADASAFARFMRTVTRQRA
VENHPLNARLVFEELFIGYRDLFA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=345, Percent_Identity=28.9855072463768, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786676, Length=171, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37461; Mature: 37461

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPWQADDWNRLQQLRGHWPHALLLHGQAGIGKLRFAQHLAQGLLCEAQQANGEPCGAC
CCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEE
VACNWFAQGNHPDYRIIVPEALAAEAGLNNAADEKADKADADEGKKTRTPSKEIKIEQIR
EEEEECCCCCCCCEEEECCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GLLDFVGVGSHRGGARVVVLYPAEALNIAAANALLKTLEEPPAGVVFLMVSARIDRLLPT
HHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHH
IISRCRQWPMTMPAPDVATRWLAAQGVAEASALLAEAGGAPLTALALASDENRALRDWTL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
KQLAAGPDCDAFACGEALQKLPVPLVLGWLQRWMYDLLAQRTAGAPRYFPQASAALTRCA
HHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SQADASAFARFMRTVTRQRAVENHPLNARLVFEELFIGYRDLFA
HHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYPWQADDWNRLQQLRGHWPHALLLHGQAGIGKLRFAQHLAQGLLCEAQQANGEPCGAC
CCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEE
VACNWFAQGNHPDYRIIVPEALAAEAGLNNAADEKADKADADEGKKTRTPSKEIKIEQIR
EEEEECCCCCCCCEEEECCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GLLDFVGVGSHRGGARVVVLYPAEALNIAAANALLKTLEEPPAGVVFLMVSARIDRLLPT
HHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHH
IISRCRQWPMTMPAPDVATRWLAAQGVAEASALLAEAGGAPLTALALASDENRALRDWTL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
KQLAAGPDCDAFACGEALQKLPVPLVLGWLQRWMYDLLAQRTAGAPRYFPQASAALTRCA
HHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SQADASAFARFMRTVTRQRAVENHPLNARLVFEELFIGYRDLFA
HHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]