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The map label for this gene is thlA [H]

Identifier: 91783481

GI number: 91783481

Start: 2326802

End: 2327986

Strand: Direct

Name: thlA [H]

Synonym: Bxe_A2335

Alternate gene names: 91783481

Gene position: 2326802-2327986 (Clockwise)

Preceding gene: 91783480

Following gene: 91783482

Centisome position: 47.53

GC content: 66.58

Gene sequence:

>1185_bases
ATGCAACGGGACGTAGTGGTGGTAAGCGGCGTGCGCACGGCAATCGGCACTTTCGGCGGCAGTCTGAAAGATATTCCGCC
GACCGATCTCGGCGCACGCGTGGTCCGTGAAGCGCTGGCGCGCGCCAGTGTGTCGGGCGACGAAGTCGGCCACGTGGTGT
TCGGCAACGTCGTGCATACGGAACCGAAAGACATGTATCTGGCGCGAGTCGCCGCGATCAACGGCGGCGTCGCGCAACAC
GTGCCGGCCTTGACCGTCAACCGCCTGTGCGGCTCCGGCCTGCAGGCGATCGTCTCGGCCGCGCAAAGCGTGCTGCTTGG
CGACGCCGACATCGCCATCGGCGGCGGCGCGGAGAACATGAGCCGCGCACCGTACTCCATGCCTGCCGCGCGCTTCGGTC
AGCGCATGGGCGACGCGCGCCTCGTCGACATGATGGTCGGCGCGCTGAACGACCCGTTCCAGTCGATCCACATGGGTGTG
ACCGCGGAGAACGTCGCGCGCAAGTACGATATCTCGCGTGAAACGCAGGACGCACTCGCGCTCGAATCGCATCGCCGCGC
GGCCAACGCGATCACGAGCGGATACTTCAACGAACAGATCCTGCCTATCACGATCCCGTCGAAGAAAGGCGACATCGTGT
TCGACACGGACGAGCATGCGCGCATGAGCGCGTCGGCTGAAGATTTCTCCAGGCTGAAGCCCGTGTTCGCGAAAGAAAAC
GGCACGGTGACGGCCGGCAACGCCTCGGGCATCAACGACGCCGCCGCGGCCGTCGTGCTGATGGAGCGCGGCATCGCCGA
ACAGCGTGGCATCAAACCGCTGGCGCGGCTGGTCTCGTACGCGCATGCGGGCGTCGATCCCGCGTACATGGGCATCGGCC
CGGTGCCGGCCACCCGCAAGGCGCTCGAACGGGCCGGACTGACGATCGCCGACCTCGACGTGATCGAGGCGAACGAAGCG
TTCGCCGCGCAGGCCTGCGCGGTCAGCAAGGAACTGGGCTTCGATCCCGCCAAGGTCAATCCGAACGGCTCAGGCATTTC
GCTTGGCCACCCGATCGGCGCGACCGGTGCGCTCATCACCGTCAAGGCGCTGTATGAGCTGCAGCGGGTCGGCGGCCGCT
ACGCGCTGGTCACGATGTGCATCGGCGGCGGCCAGGGTATCGCGGCGATCTTCGAGCGGATCTGA

Upstream 100 bases:

>100_bases
GCGCGAGACGGTCGAACAACTTCTGGCGGACTGAACACGCGCGGTGATGCGCGGCCCGCCGGGGAAAAATCATCTTGAGA
GACGGAACAGGGAGGCAGCG

Downstream 100 bases:

>100_bases
AGCGCTCACGTAGCAGGCAGTATCGAGTCAGTATCGGGCAATGAGGAAGGAACGGATGCGGGAACTGAAAGCGATGATAG
GTGCGAGGCTTCGGCGCGGT

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MQRDVVVVSGVRTAIGTFGGSLKDIPPTDLGARVVREALARASVSGDEVGHVVFGNVVHTEPKDMYLARVAAINGGVAQH
VPALTVNRLCGSGLQAIVSAAQSVLLGDADIAIGGGAENMSRAPYSMPAARFGQRMGDARLVDMMVGALNDPFQSIHMGV
TAENVARKYDISRETQDALALESHRRAANAITSGYFNEQILPITIPSKKGDIVFDTDEHARMSASAEDFSRLKPVFAKEN
GTVTAGNASGINDAAAAVVLMERGIAEQRGIKPLARLVSYAHAGVDPAYMGIGPVPATRKALERAGLTIADLDVIEANEA
FAAQACAVSKELGFDPAKVNPNGSGISLGHPIGATGALITVKALYELQRVGGRYALVTMCIGGGQGIAAIFERI

Sequences:

>Translated_394_residues
MQRDVVVVSGVRTAIGTFGGSLKDIPPTDLGARVVREALARASVSGDEVGHVVFGNVVHTEPKDMYLARVAAINGGVAQH
VPALTVNRLCGSGLQAIVSAAQSVLLGDADIAIGGGAENMSRAPYSMPAARFGQRMGDARLVDMMVGALNDPFQSIHMGV
TAENVARKYDISRETQDALALESHRRAANAITSGYFNEQILPITIPSKKGDIVFDTDEHARMSASAEDFSRLKPVFAKEN
GTVTAGNASGINDAAAAVVLMERGIAEQRGIKPLARLVSYAHAGVDPAYMGIGPVPATRKALERAGLTIADLDVIEANEA
FAAQACAVSKELGFDPAKVNPNGSGISLGHPIGATGALITVKALYELQRVGGRYALVTMCIGGGQGIAAIFERI
>Mature_394_residues
MQRDVVVVSGVRTAIGTFGGSLKDIPPTDLGARVVREALARASVSGDEVGHVVFGNVVHTEPKDMYLARVAAINGGVAQH
VPALTVNRLCGSGLQAIVSAAQSVLLGDADIAIGGGAENMSRAPYSMPAARFGQRMGDARLVDMMVGALNDPFQSIHMGV
TAENVARKYDISRETQDALALESHRRAANAITSGYFNEQILPITIPSKKGDIVFDTDEHARMSASAEDFSRLKPVFAKEN
GTVTAGNASGINDAAAAVVLMERGIAEQRGIKPLARLVSYAHAGVDPAYMGIGPVPATRKALERAGLTIADLDVIEANEA
FAAQACAVSKELGFDPAKVNPNGSGISLGHPIGATGALITVKALYELQRVGGRYALVTMCIGGGQGIAAIFERI

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=45.7800511508951, Blast_Score=357, Evalue=8e-99,
Organism=Homo sapiens, GI167614485, Length=393, Percent_Identity=45.29262086514, Blast_Score=352, Evalue=2e-97,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=40.0503778337531, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=41.6666666666667, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=35.0588235294118, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=31.4578005115089, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1788554, Length=398, Percent_Identity=50.7537688442211, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=48.2142857142857, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=45.5445544554455, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI48994986, Length=397, Percent_Identity=40.3022670025189, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788683, Length=409, Percent_Identity=36.6748166259169, Blast_Score=227, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI133906874, Length=393, Percent_Identity=46.5648854961832, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI25147385, Length=393, Percent_Identity=34.0966921119593, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17535921, Length=398, Percent_Identity=33.4170854271357, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=31.3679245283019, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17535917, Length=396, Percent_Identity=28.030303030303, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6325229, Length=404, Percent_Identity=40.3465346534653, Blast_Score=275, Evalue=9e-75,
Organism=Saccharomyces cerevisiae, GI6322031, Length=404, Percent_Identity=37.1287128712871, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=45.4081632653061, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=43.6548223350254, Blast_Score=316, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24640423, Length=393, Percent_Identity=40.9669211195929, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI17137578, Length=428, Percent_Identity=31.5420560747664, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41040; Mature: 41040

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRDVVVVSGVRTAIGTFGGSLKDIPPTDLGARVVREALARASVSGDEVGHVVFGNVVHT
CCCCEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCEEEC
EPKDMYLARVAAINGGVAQHVPALTVNRLCGSGLQAIVSAAQSVLLGDADIAIGGGAENM
CCHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCC
SRAPYSMPAARFGQRMGDARLVDMMVGALNDPFQSIHMGVTAENVARKYDISRETQDALA
CCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHCCCCHHHHHHH
LESHRRAANAITSGYFNEQILPITIPSKKGDIVFDTDEHARMSASAEDFSRLKPVFAKEN
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCHHHHCCCHHHHHHHCCCEECCC
GTVTAGNASGINDAAAAVVLMERGIAEQRGIKPLARLVSYAHAGVDPAYMGIGPVPATRK
CEEEECCCCCCHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCCHHCCCCCCCHHHH
ALERAGLTIADLDVIEANEAFAAQACAVSKELGFDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCEEEECEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCHHHHH
VKALYELQRVGGRYALVTMCIGGGQGIAAIFERI
HHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure
MQRDVVVVSGVRTAIGTFGGSLKDIPPTDLGARVVREALARASVSGDEVGHVVFGNVVHT
CCCCEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCEEEC
EPKDMYLARVAAINGGVAQHVPALTVNRLCGSGLQAIVSAAQSVLLGDADIAIGGGAENM
CCHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCC
SRAPYSMPAARFGQRMGDARLVDMMVGALNDPFQSIHMGVTAENVARKYDISRETQDALA
CCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHCCCCHHHHHHH
LESHRRAANAITSGYFNEQILPITIPSKKGDIVFDTDEHARMSASAEDFSRLKPVFAKEN
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCHHHHCCCHHHHHHHCCCEECCC
GTVTAGNASGINDAAAAVVLMERGIAEQRGIKPLARLVSYAHAGVDPAYMGIGPVPATRK
CEEEECCCCCCHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCCHHCCCCCCCHHHH
ALERAGLTIADLDVIEANEAFAAQACAVSKELGFDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCEEEECEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCHHHHH
VKALYELQRVGGRYALVTMCIGGGQGIAAIFERI
HHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]