Click here to switch to the map view.

The map label for this gene is pit [H]

Identifier: 91783420

GI number: 91783420

Start: 2258965

End: 2259975

Strand: Direct

Name: pit [H]

Synonym: Bxe_A2395

Alternate gene names: 91783420

Gene position: 2258965-2259975 (Clockwise)

Preceding gene: 91783419

Following gene: 91783432

Centisome position: 46.14

GC content: 63.2

Gene sequence:

>1011_bases
ATGCAATCGATACAACTCGCAATCTGGGTCGTCACTGGCCTGGTCGCCGTCGCGCTGATTTTCGACTTCATGAACGGCTT
CCACGACGCGGCGAATTCGATCGCCACGGTCGTCTCGACCGGCGTGCTGAAACCGCAGCAGGCGGTTGCCTTTGCCGCGG
CGTTCAACGTCATCGCGTATTTCGTGTTCCATCTGAAAGTGGCGGCGACCGTCGGCAAAGGCACGATCGATCCGAACATC
GTCGACCATTACGTGATTTTCGGCGCGCTGGTCGGCGCGATCGGCTGGAACATCATCACCTGGCATTACGGCATTCCGTC
GAGCTCGTCGCATGCGCTGATCGGCGGCCTGGTGGGCGCGGCGCTCGCCAAGTCGGGCTGGGGTTCGCTCAATGTCGACG
GCCTGATGAAGACGGTGGCCTTCATCTTCATTTCGCCGCTGCTCGGCTTCGTGCTCGGCTCGTTCTTCATGCTGGCGGTG
TCGTGGATCTATTTCCGCACGCCGCCGAGCAAGGTGGACCGGCGTTTCCGGCGTCTGCAACTGGTGTCCGCGGGCTTGTA
TAGCCTCGGCCACGGCGGTAACGACGCGCAGAAAACCATCGGCATCATCTGGATGCTGCTGATCGCGACCGGCTATGCGT
CGTCGATCGCGGATGCGCCGCCGCTGTGGGTGATCGGCGGCTGCTATCTCTCCATGGGCATCGGTACGCTGTTCGGCGGC
TGGCGGATCGTCCGCACGATGGGTCAGAAGATCACCAAGCTCAAGCCGGTCGGCGGTTTTTGCGCGGAGTCGGGCGGCGC
GATCACGCTATTTACCGCCTCGTGGCTCGGCATTCCGGTGTCCACCACACATACGATTACCGGCGCGATCGTCGGTGTCG
GCGCGACGCAAAAGCTGAGTGCGGTGCGCTGGGGCGTGGCCGGCAACATCGTCTGGGCGTGGATTCTGACGATTCCGGCT
TCTGCGGCGCTCGCCGGGGTTGCGTGGTGGCTCGGCCACCGTTTCCTGTAG

Upstream 100 bases:

>100_bases
TGATCAAGCTGAAGGCGATCTACGAGTTGCTCGAAACGATCACGGACAAATGTGAAGATGTAGCGAACATCATCGAAGGC
ATCGTGCTGGAAAACGCATA

Downstream 100 bases:

>100_bases
CCGCGGAAAACGCGGAAAACGCACGACGCGAGCGGCAGCGCCGCCTGCCGCCCGCTCCATTGCAGGGTTCTAGCGGGTTT
CGAAGCGCGCCTAGATCAGC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MQSIQLAIWVVTGLVAVALIFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAAFNVIAYFVFHLKVAATVGKGTIDPNI
VDHYVIFGALVGAIGWNIITWHYGIPSSSSHALIGGLVGAALAKSGWGSLNVDGLMKTVAFIFISPLLGFVLGSFFMLAV
SWIYFRTPPSKVDRRFRRLQLVSAGLYSLGHGGNDAQKTIGIIWMLLIATGYASSIADAPPLWVIGGCYLSMGIGTLFGG
WRIVRTMGQKITKLKPVGGFCAESGGAITLFTASWLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWILTIPA
SAALAGVAWWLGHRFL

Sequences:

>Translated_336_residues
MQSIQLAIWVVTGLVAVALIFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAAFNVIAYFVFHLKVAATVGKGTIDPNI
VDHYVIFGALVGAIGWNIITWHYGIPSSSSHALIGGLVGAALAKSGWGSLNVDGLMKTVAFIFISPLLGFVLGSFFMLAV
SWIYFRTPPSKVDRRFRRLQLVSAGLYSLGHGGNDAQKTIGIIWMLLIATGYASSIADAPPLWVIGGCYLSMGIGTLFGG
WRIVRTMGQKITKLKPVGGFCAESGGAITLFTASWLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWILTIPA
SAALAGVAWWLGHRFL
>Mature_336_residues
MQSIQLAIWVVTGLVAVALIFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAAFNVIAYFVFHLKVAATVGKGTIDPNI
VDHYVIFGALVGAIGWNIITWHYGIPSSSSHALIGGLVGAALAKSGWGSLNVDGLMKTVAFIFISPLLGFVLGSFFMLAV
SWIYFRTPPSKVDRRFRRLQLVSAGLYSLGHGGNDAQKTIGIIWMLLIATGYASSIADAPPLWVIGGCYLSMGIGTLFGG
WRIVRTMGQKITKLKPVGGFCAESGGAITLFTASWLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWILTIPA
SAALAGVAWWLGHRFL

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=168, Percent_Identity=36.3095238095238, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI5803173, Length=151, Percent_Identity=35.7615894039735, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1789360, Length=231, Percent_Identity=31.6017316017316, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=31.7972350230415, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17539280, Length=177, Percent_Identity=34.4632768361582, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25146401, Length=157, Percent_Identity=29.9363057324841, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32566716, Length=169, Percent_Identity=27.810650887574, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71981576, Length=161, Percent_Identity=26.0869565217391, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17557328, Length=169, Percent_Identity=32.5443786982249, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17536725, Length=180, Percent_Identity=29.4444444444444, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319773, Length=174, Percent_Identity=31.6091954022989, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356511, Length=198, Percent_Identity=32.8282828282828, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35508; Mature: 35508

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSIQLAIWVVTGLVAVALIFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAAFNVIAY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FVFHLKVAATVGKGTIDPNIVDHYVIFGALVGAIGWNIITWHYGIPSSSSHALIGGLVGA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHH
ALAKSGWGSLNVDGLMKTVAFIFISPLLGFVLGSFFMLAVSWIYFRTPPSKVDRRFRRLQ
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LVSAGLYSLGHGGNDAQKTIGIIWMLLIATGYASSIADAPPLWVIGGCYLSMGIGTLFGG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEHHHHHHHHHHHHHHH
WRIVRTMGQKITKLKPVGGFCAESGGAITLFTASWLGIPVSTTHTITGAIVGVGATQKLS
HHHHHHHHHHHHHCCCCCCHHCCCCCEEEEEEEHHCCCCCCCCHHHHHHEEECCCHHHHH
AVRWGVAGNIVWAWILTIPASAALAGVAWWLGHRFL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSIQLAIWVVTGLVAVALIFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAAFNVIAY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FVFHLKVAATVGKGTIDPNIVDHYVIFGALVGAIGWNIITWHYGIPSSSSHALIGGLVGA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHH
ALAKSGWGSLNVDGLMKTVAFIFISPLLGFVLGSFFMLAVSWIYFRTPPSKVDRRFRRLQ
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LVSAGLYSLGHGGNDAQKTIGIIWMLLIATGYASSIADAPPLWVIGGCYLSMGIGTLFGG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEHHHHHHHHHHHHHHH
WRIVRTMGQKITKLKPVGGFCAESGGAITLFTASWLGIPVSTTHTITGAIVGVGATQKLS
HHHHHHHHHHHHHCCCCCCHHCCCCCEEEEEEEHHCCCCCCCCHHHHHHEEECCCHHHHH
AVRWGVAGNIVWAWILTIPASAALAGVAWWLGHRFL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]