Click here to switch to the map view.

The map label for this gene is iciA [H]

Identifier: 91782861

GI number: 91782861

Start: 1647733

End: 1648626

Strand: Direct

Name: iciA [H]

Synonym: Bxe_A2962

Alternate gene names: 91782861

Gene position: 1647733-1648626 (Clockwise)

Preceding gene: 91782859

Following gene: 91782866

Centisome position: 33.66

GC content: 67.23

Gene sequence:

>894_bases
ATGCTCGACTATGCGTTGCTCGACGCACTGGCCGCCGTGGTGCGCCACGGCTCGTTCGATCGCGCCGCCGGCGAACTCAA
CGTGACGCCGTCCGCGGTGTCGCAGCGGGTCAAGCTGCTGGAAGAGCGCGTGGGCAGCGTGCTGGTGAAGCGCGGCCAGC
CATGCGTCGCGACCACCTCGGGCGCGCTGCTGTGTCGCCATACCGAACGCGTGCAATTGCTGGAAGCGGAGCTGACCGGC
CGCCTGCCGGCGCTGCCCGGCGCGCTGGTCGAACCGTGGCCCGCGCTGCGCGTGGCCGTCAACGACGACAGCGTGGGCAC
CTGGTTTATCGACGCGGTTGCGCAATTCTGCGTAGAGCGGGAAATGCTGCTCGATCTGGTGATCGACGATCAGGACCATA
CCGCGCAGCGGATTCGCGACGGCAGCGTGCAAGGCGCGGTCACCACGCAGGCCGAGCCGGTGCAGGGCTGCCGCTCCACG
CGTCTTGGGCGGATGCGCTATCTGGCGGTGTGTTCGCCGGACTTCCTCGCACGTCACTTTGCCGGCGGTGTTACGCGCGA
GAGTTTGCGGCGCACGCCGTGCGTCGACTTCAATCCGAAAGACCAGTTGCAAAAGCGCTTCATGCGCCGCATCACGCGCG
CGGAACTCGATCCGCCGCTGCACTGGATTCCGCATGTCGCCGGTTTCTTGCGCGCCTGCGTGACGGGCCTTGGGTGGGGC
ATGTGCCCGGAACGCATGATCGCCCCGCATCTGGCGAGCGGCGAGCTGGTGGAAATGGCGCCCGGCAAGCCCATCGATGT
CGACCTGTACTGGCAGAGCTGGCGTTTGTCGATTGGCTGGCTCGACGATTTCGGCGCGGTGCTGCGCAAGCGGGCAGCGG
AGTTTCTGGACTGA

Upstream 100 bases:

>100_bases
CATAATCGATCCAGTAGACGGGAGAAGCGAACGGGGCTAGTCTGCGGGTTTACTTGTCGTAAGAGAAGCTAATTTACTTA
ATGCCGATTAAGGAACGCTA

Downstream 100 bases:

>100_bases
AAGTCCAGCACGCACGATCAGGTGGCCGGCGAAAATGCCGCATACGTGCCGGTGCGTGCTTCGCTCAACTGCCGGGCAGA
TGCTTCGGAAAACGCAGACT

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLDYALLDALAAVVRHGSFDRAAGELNVTPSAVSQRVKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVQLLEAELTG
RLPALPGALVEPWPALRVAVNDDSVGTWFIDAVAQFCVEREMLLDLVIDDQDHTAQRIRDGSVQGAVTTQAEPVQGCRST
RLGRMRYLAVCSPDFLARHFAGGVTRESLRRTPCVDFNPKDQLQKRFMRRITRAELDPPLHWIPHVAGFLRACVTGLGWG
MCPERMIAPHLASGELVEMAPGKPIDVDLYWQSWRLSIGWLDDFGAVLRKRAAEFLD

Sequences:

>Translated_297_residues
MLDYALLDALAAVVRHGSFDRAAGELNVTPSAVSQRVKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVQLLEAELTG
RLPALPGALVEPWPALRVAVNDDSVGTWFIDAVAQFCVEREMLLDLVIDDQDHTAQRIRDGSVQGAVTTQAEPVQGCRST
RLGRMRYLAVCSPDFLARHFAGGVTRESLRRTPCVDFNPKDQLQKRFMRRITRAELDPPLHWIPHVAGFLRACVTGLGWG
MCPERMIAPHLASGELVEMAPGKPIDVDLYWQSWRLSIGWLDDFGAVLRKRAAEFLD
>Mature_297_residues
MLDYALLDALAAVVRHGSFDRAAGELNVTPSAVSQRVKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVQLLEAELTG
RLPALPGALVEPWPALRVAVNDDSVGTWFIDAVAQFCVEREMLLDLVIDDQDHTAQRIRDGSVQGAVTTQAEPVQGCRST
RLGRMRYLAVCSPDFLARHFAGGVTRESLRRTPCVDFNPKDQLQKRFMRRITRAELDPPLHWIPHVAGFLRACVTGLGWG
MCPERMIAPHLASGELVEMAPGKPIDVDLYWQSWRLSIGWLDDFGAVLRKRAAEFLD

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=280, Percent_Identity=35.7142857142857, Blast_Score=151, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32944; Mature: 32944

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDYALLDALAAVVRHGSFDRAAGELNVTPSAVSQRVKLLEERVGSVLVKRGQPCVATTS
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GALLCRHTERVQLLEAELTGRLPALPGALVEPWPALRVAVNDDSVGTWFIDAVAQFCVER
CCEEEECHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
EMLLDLVIDDQDHTAQRIRDGSVQGAVTTQAEPVQGCRSTRLGRMRYLAVCSPDFLARHF
HHHHHHHCCCCCHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCHHHEEEECCHHHHHHHH
AGGVTRESLRRTPCVDFNPKDQLQKRFMRRITRAELDPPLHWIPHVAGFLRACVTGLGWG
HCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
MCPERMIAPHLASGELVEMAPGKPIDVDLYWQSWRLSIGWLDDFGAVLRKRAAEFLD
CCHHHHHCCCCCCCCEEEECCCCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDYALLDALAAVVRHGSFDRAAGELNVTPSAVSQRVKLLEERVGSVLVKRGQPCVATTS
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GALLCRHTERVQLLEAELTGRLPALPGALVEPWPALRVAVNDDSVGTWFIDAVAQFCVER
CCEEEECHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
EMLLDLVIDDQDHTAQRIRDGSVQGAVTTQAEPVQGCRSTRLGRMRYLAVCSPDFLARHF
HHHHHHHCCCCCHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCHHHEEEECCHHHHHHHH
AGGVTRESLRRTPCVDFNPKDQLQKRFMRRITRAELDPPLHWIPHVAGFLRACVTGLGWG
HCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
MCPERMIAPHLASGELVEMAPGKPIDVDLYWQSWRLSIGWLDDFGAVLRKRAAEFLD
CCHHHHHCCCCCCCCEEEECCCCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA