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The map label for this gene is pnp [H]

Identifier: 91782608

GI number: 91782608

Start: 1377270

End: 1379420

Strand: Direct

Name: pnp [H]

Synonym: Bxe_A3218

Alternate gene names: 91782608

Gene position: 1377270-1379420 (Clockwise)

Preceding gene: 91782607

Following gene: 91782609

Centisome position: 28.13

GC content: 62.39

Gene sequence:

>2151_bases
ATGACTATGTTCAATAAGATCGTCAAAGAATTTCAGTGGGGACAACATAAGGTTCGCCTCGAAACCGGCGAAATCGCTCG
TCAGGCAAGCGGCGCCGTGATCGTCGACGTCGAAGATACCGTGGTGCTGGCCACCGTGGTCGGCGCCAGGACGGCCAAGC
CGGGTCAGGACTTTTTCCCGCTGACCGTCGACTACCTCGAAAAGACTTACGCAGCCGGCAAGATTCCGGGTGGCTTCTTC
CGCCGCGAAGGCCGTCCGTCGGAAGGCGAAACGCTGATCTCGCGCCTGATCGACCGTCCGCTGCGTCCGCTGTTCCCGGA
AGGCTTCTACAACGAAGTCCAGGTCGTGATCCACGTCCTTTCGCTGAACCCCGAAATCCCCGCTGACATTCCCGCGCTGA
TCGGCGCGTCGGCGGCGCTCGCCGTGTCCGGTCTGCCGTTCAACGGCCCGGTCGGCGCAGCACGCGTGGCCTACATCAAC
AACGAATACGTGTTGAACCCGACGCGTCCGCAAATGAAGGAATCGGCGCTCGACCTGATCGTGGCCGGTACGGAACGCGC
GGTGCTGATGGTGGAATCGGAAGCGCAGCAACTGAGCGAAGAAGTGATGCTCGGCGGCGTGGTGTTCGGTCACGAACAGA
TGCAGATCGCGATCGACGCGATCCACGATCTGGTCCGCGAAGGCGGCAAGCCGGAATGGGATTGGCAGCCGGCGGCGAAG
AACGAGCCGTTGATCGCCCGCGTAACGGAACTGGCGCAAGCCGATCTGCTCGCCGCTTATCAATTGCGCGACAAGCAGGC
TCGTTCGGCCAAGCTGAAGGAAATCTACGCAGCCACGTCGGCAAAGCTGGAAGAAGACGCGGCAGCAGGCGGCACGGTAG
CGGCCGACAAGGCAACTGTCGGCAACGTGCTGTTCGACATCGAAGCGAAGATCGTCCGTACGCAGATCCTGAACGGCGAA
CCGCGTATCGACGGGCGCGACACGCGTACCGTGCGCCCGATCGAAATCCGTACCGGCGTGCTGCCGCGTACGCACGGTTC
GGCACTCTTCACGCGCGGCGAAACGCAGGCGCTGGTGGTGGCCACGCTGGGCACGAAGGGCGACGAGCAGAACATCGACG
CGCTCGAAGGCGAATACCGCGAACGCTTCATGCTCCACTACAACATGCCGCCGTTCGCGACCGGCGAAACGGGCCGCGTC
GGTTCGCCGAAGCGCCGTGAAATCGGTCACGGCCGTCTGGCCAAGCGCGCGCTGGCCGCGTGCCTGCCGAGCGCGGACGA
GTTCGGCTACTCGATCCGCGTCGTGTCGGAAATCACGGAATCGAACGGTTCCTCGTCGATGGCTTCGGTGTGCGGCGGCT
GCCTCGCGCTGATGGACGCCGGCGTGCCGATGAAGGCGCACGTCGCCGGCATCGCAATGGGCCTGATCCTGGAAGGCAAC
AAGTTTGCCGTGCTGACCGACATCCTCGGCGACGAAGATCACCTCGGCGACATGGACTTCAAGGTGGCGGGCACCGAGCA
AGGCGTGACCGCGCTGCAAATGGACATCAAGATCCAGGGCATCACCAGGGAAATCATGCAGGTCGCCCTCGCGCAAGCCA
AGGAAGGCCGTTTGCACATCCTCGGCAAGATGACCTCGGCTGTCTCGGGCGCGAACACGGTGCTGTCGGACTACGCACCG
CGCATGATCACCATCAAGATCAATCCGGAAAAAATCCGCGACGTGATCGGCAAGGGTGGTTCGGTGATCCGCGCGCTGAC
CGAAGAAACCGGCACGACGATCGATATTTCGGACGACGGCGTCGTCACCATCGCCAGCACGAGCAGTGAAGGCATGGCGG
AAGCGAAGAAGCGTATCGAGAACATCACGCTGGAAGTCGAAGTGGGTCAGGTGTACGAAGGCACCGTGCTCAAGCTGCTC
GATTTCGGCGCGATCGTGAACATCCTGCCGGGCAAGGACGGTCTGCTGCACATTTCCGAAATCGCCAACGAGCGTATCAA
GGACATCAACGACTATCTGAAAGATGGCCAGCAGGTGAAGGTCAAGGTCATCCAGACGGACGAAAAGGGCCGCGTGCGTT
TGTCGGCCAAGGCGTTGCTGAACGACGCCGCGAACGGCGCGCCGCAAGGCGAGCCGACGCCGCAGCAGTAA

Upstream 100 bases:

>100_bases
GGTTCTCTGGAATGGCATAACACTACTCTTCCCATGCGGCGCCGGTCCTGGCGTTGCCGCGCCGCTTCTGGTTCGCGCGG
CATTACGAAGAGGAAAAGCA

Downstream 100 bases:

>100_bases
TGCGGTAGCGGACAACGGCCGGCAGCGTGAATGCGCGCCGGCCGTTTTTCATGAAGGGTTCAGGTGAATTGCGCTGCACG
CGGGCGCCCCGGAGGAAATC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 716; Mature: 715

Protein sequence:

>716_residues
MTMFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGARTAKPGQDFFPLTVDYLEKTYAAGKIPGGFF
RREGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVLSLNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYIN
NEYVLNPTRPQMKESALDLIVAGTERAVLMVESEAQQLSEEVMLGGVVFGHEQMQIAIDAIHDLVREGGKPEWDWQPAAK
NEPLIARVTELAQADLLAAYQLRDKQARSAKLKEIYAATSAKLEEDAAAGGTVAADKATVGNVLFDIEAKIVRTQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQNIDALEGEYRERFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALAACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITREIMQVALAQAKEGRLHILGKMTSAVSGANTVLSDYAP
RMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIENITLEVEVGQVYEGTVLKLL
DFGAIVNILPGKDGLLHISEIANERIKDINDYLKDGQQVKVKVIQTDEKGRVRLSAKALLNDAANGAPQGEPTPQQ

Sequences:

>Translated_716_residues
MTMFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGARTAKPGQDFFPLTVDYLEKTYAAGKIPGGFF
RREGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVLSLNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYIN
NEYVLNPTRPQMKESALDLIVAGTERAVLMVESEAQQLSEEVMLGGVVFGHEQMQIAIDAIHDLVREGGKPEWDWQPAAK
NEPLIARVTELAQADLLAAYQLRDKQARSAKLKEIYAATSAKLEEDAAAGGTVAADKATVGNVLFDIEAKIVRTQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQNIDALEGEYRERFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALAACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITREIMQVALAQAKEGRLHILGKMTSAVSGANTVLSDYAP
RMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIENITLEVEVGQVYEGTVLKLL
DFGAIVNILPGKDGLLHISEIANERIKDINDYLKDGQQVKVKVIQTDEKGRVRLSAKALLNDAANGAPQGEPTPQQ
>Mature_715_residues
TMFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGARTAKPGQDFFPLTVDYLEKTYAAGKIPGGFFR
REGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVLSLNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYINN
EYVLNPTRPQMKESALDLIVAGTERAVLMVESEAQQLSEEVMLGGVVFGHEQMQIAIDAIHDLVREGGKPEWDWQPAAKN
EPLIARVTELAQADLLAAYQLRDKQARSAKLKEIYAATSAKLEEDAAAGGTVAADKATVGNVLFDIEAKIVRTQILNGEP
RIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQNIDALEGEYRERFMLHYNMPPFATGETGRVG
SPKRREIGHGRLAKRALAACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGNK
FAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITREIMQVALAQAKEGRLHILGKMTSAVSGANTVLSDYAPR
MITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIENITLEVEVGQVYEGTVLKLLD
FGAIVNILPGKDGLLHISEIANERIKDINDYLKDGQQVKVKVIQTDEKGRVRLSAKALLNDAANGAPQGEPTPQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=736, Percent_Identity=37.6358695652174, Blast_Score=438, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=696, Percent_Identity=64.9425287356322, Blast_Score=923, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=714, Percent_Identity=33.1932773109244, Blast_Score=332, Evalue=5e-91,
Organism=Drosophila melanogaster, GI281362905, Length=705, Percent_Identity=35.6028368794326, Blast_Score=411, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24651641, Length=705, Percent_Identity=35.6028368794326, Blast_Score=411, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24651643, Length=705, Percent_Identity=35.6028368794326, Blast_Score=411, Evalue=1e-114,
Organism=Drosophila melanogaster, GI161079377, Length=656, Percent_Identity=35.2134146341463, Blast_Score=375, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77091; Mature: 76960

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGARTAKPGQDFFP
CCHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEHHCCCCCCCCCCCCC
LTVDYLEKTYAAGKIPGGFFRREGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVL
CCHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
SLNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYINNEYVLNPTRPQMKESALDLI
HCCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCEEEECCCCCHHHHHHEEEE
VAGTERAVLMVESEAQQLSEEVMLGGVVFGHEQMQIAIDAIHDLVREGGKPEWDWQPAAK
EECCCEEEEEEEHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NEPLIARVTELAQADLLAAYQLRDKQARSAKLKEIYAATSAKLEEDAAAGGTVAADKATV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEECCCHHH
GNVLFDIEAKIVRTQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
CCEEEEEHHHEEEHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQNIDALEGEYRERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALAA
EEECCCCCCCCCHHHCCHHHCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCCHHCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITREIMQVALAQAKEGRLHI
CEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCCEEE
LGKMTSAVSGANTVLSDYAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
EEHHHHHHCCCHHHHHCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTSSEGMAEAKKRIENITLEVEVGQVYEGTVLKLLDFGAIVNILPGKDGLLHISE
EEEEECCCCCHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCEEHHH
IANERIKDINDYLKDGQQVKVKVIQTDEKGRVRLSAKALLNDAANGAPQGEPTPQQ
HHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGARTAKPGQDFFP
CHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEHHCCCCCCCCCCCCC
LTVDYLEKTYAAGKIPGGFFRREGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVL
CCHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
SLNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYINNEYVLNPTRPQMKESALDLI
HCCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCEEEECCCCCHHHHHHEEEE
VAGTERAVLMVESEAQQLSEEVMLGGVVFGHEQMQIAIDAIHDLVREGGKPEWDWQPAAK
EECCCEEEEEEEHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NEPLIARVTELAQADLLAAYQLRDKQARSAKLKEIYAATSAKLEEDAAAGGTVAADKATV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEECCCHHH
GNVLFDIEAKIVRTQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
CCEEEEEHHHEEEHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQNIDALEGEYRERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALAA
EEECCCCCCCCCHHHCCHHHCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCCHHCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITREIMQVALAQAKEGRLHI
CEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCCEEE
LGKMTSAVSGANTVLSDYAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
EEHHHHHHCCCHHHHHCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTSSEGMAEAKKRIENITLEVEVGQVYEGTVLKLLDFGAIVNILPGKDGLLHISE
EEEEECCCCCHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCEEHHH
IANERIKDINDYLKDGQQVKVKVIQTDEKGRVRLSAKALLNDAANGAPQGEPTPQQ
HHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA