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The map label for this gene is leuA [H]

Identifier: 91782603

GI number: 91782603

Start: 1370185

End: 1371732

Strand: Direct

Name: leuA [H]

Synonym: Bxe_A3225

Alternate gene names: 91782603

Gene position: 1370185-1371732 (Clockwise)

Preceding gene: 91782601

Following gene: 91782607

Centisome position: 27.99

GC content: 60.98

Gene sequence:

>1548_bases
ATGTCCGACAAACTGATCATATTCGACACCACGTTGCGAGACGGCGAACAATCGCCTGGCGCATCGATGACGAAAGAAGA
AAAAATCCGTATCGCGAAGCAACTCGAACGGATGAAGGTGGACGTGATCGAAGCAGGCTTCGCGGCAAGCTCGAACGGCG
ACTTCGATTCGATCCATACCATCGCGGGCCTGATCAAGGACAGCACGATCTGCTCGCTGGCCCGCGCGAACGACAAGGAC
ATTCAGCGCGCGGCCGACGCGCTCAAACCGGCCGACCGCTTCCGCATTCACACGTTTATCGCAACTTCGCCGCTGCACAT
GGAAAAGAAGCTGCGCATGACGCCGGACCAGGTGTTCGAGCAGGCGAAGCTGGCAGTGCGTTTCGCCCGCAAATTCACCG
ACGACGTCGAGTTTTCGCCGGAAGACGGCAGCCGCTCGGACATGGACTTTCTGTGCCGCGTGCTGGAAGCCGTGATTGCC
GAAGGCGCGACTACCATCAATATCGCGGATACGGTCGGCTACGGCGTGCCGGAGCTGTACGGCCAGCTCGTGAAAACGCT
GCGCGAGCGCATTCCGAACTCGCATAAAGCCGTGTTCTCGGTGCATTGCCACAATGACCTCGGCATGGCGGTGGCGAATT
CGCTGGCCGGCGTGCAGATCGGCGGCGCGCGCCAGGTCGAGTGCACGATCAATGGCCTTGGCGAGCGCGCGGGCAACACG
TCGCTCGAAGAAATCGTCATGGCCGTGAAGACCCGCAGGGATTACTTCGGGCTCGATGTCGGTCTCGATACCACGCAGAT
CGTGCCGGCTTCGAAACTGGTGTCGCAGATCACCGGTTTCGTCGTGCAGCCGAACAAGGCGGTGGTCGGCGCGAACGCGT
TCGCCCATGCTTCGGGCATCCACCAGGACGGCGTGCTGAAGGCGCGCGACACCTACGAAATCATGCGCGCCGAGGACGTG
GGCTGGAGCGCGAACAAGATCGTGCTCGGCAAGCTGTCGGGCCGTAACGCGTTCAAGCAGCGTTTGCAGGAACTGGGCAT
TGCGCTGGATAGCGAAGGCGAGTTGAACACGGCGTTCGCGCGCTTCAAGGAACTGGCCGACCGCAAGTCGGAAATCTTCG
ACGAAGACATCATCGCGATCGTCACGGAAGAATCCGCCGAGGCGCAGGAGAAAGAGCACTACAAGTTCCTGTCGCTGTCG
CAACATTCGGAAACCGGCGAGCAGCCGCACGCGAAGATCGTGTTCTCCGTCGAAGGCAAGGAAGTGACCGGTGAGGCGCG
CGGCAACGGCCCGGTCGACGCCACGCTGAACGCGATCGAAACGGAAGTGGGGAGCGGTTCGGAACTGCTGCTGTACTCGG
TGAACGCGATCACCACCGGCACGCAGGCACAAGGCGAAGTGACCGTGCGTTTGTCGAAGAGCGGGCGCATCGTCAACGGC
GTGGGCACGGATCCGGATATCGTCGCGGCTTCCGCGAAAGCGTATATCTCCGCGCTCAACAAGCTGTATTCGAACGTGGA
CAAGTTGAATCCGCAACGTTCGGAGTGA

Upstream 100 bases:

>100_bases
CGTCTGCCTCGTCTGTGATCGATTATTGCCAGCGGTAGCGCAGACAGCCGAATTCCAATATCCCATCCCCACCATTGAAC
GACTCCGCAGGAGACTCGAC

Downstream 100 bases:

>100_bases
GCGAGGCAAAGGCAGCGCGCTGACGGCGCGACTGCCTGTCCGTCACAGGAGTCGCAAAAGCCCCGCGCCTTGAGTGGCGT
GGGGCTTTTGCATTGTGGGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDSIHTIAGLIKDSTICSLARANDKD
IQRAADALKPADRFRIHTFIATSPLHMEKKLRMTPDQVFEQAKLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIA
EGATTINIADTVGYGVPELYGQLVKTLRERIPNSHKAVFSVHCHNDLGMAVANSLAGVQIGGARQVECTINGLGERAGNT
SLEEIVMAVKTRRDYFGLDVGLDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWSANKIVLGKLSGRNAFKQRLQELGIALDSEGELNTAFARFKELADRKSEIFDEDIIAIVTEESAEAQEKEHYKFLSLS
QHSETGEQPHAKIVFSVEGKEVTGEARGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
VGTDPDIVAASAKAYISALNKLYSNVDKLNPQRSE

Sequences:

>Translated_515_residues
MSDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDSIHTIAGLIKDSTICSLARANDKD
IQRAADALKPADRFRIHTFIATSPLHMEKKLRMTPDQVFEQAKLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIA
EGATTINIADTVGYGVPELYGQLVKTLRERIPNSHKAVFSVHCHNDLGMAVANSLAGVQIGGARQVECTINGLGERAGNT
SLEEIVMAVKTRRDYFGLDVGLDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWSANKIVLGKLSGRNAFKQRLQELGIALDSEGELNTAFARFKELADRKSEIFDEDIIAIVTEESAEAQEKEHYKFLSLS
QHSETGEQPHAKIVFSVEGKEVTGEARGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
VGTDPDIVAASAKAYISALNKLYSNVDKLNPQRSE
>Mature_514_residues
SDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDSIHTIAGLIKDSTICSLARANDKDI
QRAADALKPADRFRIHTFIATSPLHMEKKLRMTPDQVFEQAKLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIAE
GATTINIADTVGYGVPELYGQLVKTLRERIPNSHKAVFSVHCHNDLGMAVANSLAGVQIGGARQVECTINGLGERAGNTS
LEEIVMAVKTRRDYFGLDVGLDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVG
WSANKIVLGKLSGRNAFKQRLQELGIALDSEGELNTAFARFKELADRKSEIFDEDIIAIVTEESAEAQEKEHYKFLSLSQ
HSETGEQPHAKIVFSVEGKEVTGEARGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
GTDPDIVAASAKAYISALNKLYSNVDKLNPQRSE

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=504, Percent_Identity=48.8095238095238, Blast_Score=453, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6324682, Length=546, Percent_Identity=25.2747252747253, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=559, Percent_Identity=24.865831842576, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=386, Percent_Identity=28.2383419689119, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320019, Length=382, Percent_Identity=28.2722513089005, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55859; Mature: 55727

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDSIHT
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAGLIKDSTICSLARANDKDIQRAADALKPADRFRIHTFIATSPLHMEKKLRMTPDQVFE
HHHHHHCHHHHHHHCCCHHHHHHHHHHCCCCHHEEEEEEEECCCHHHHHHCCCCHHHHHH
QAKLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIAEGATTINIADTVGYGVPELY
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHH
GQLVKTLRERIPNSHKAVFSVHCHNDLGMAVANSLAGVQIGGARQVECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVKTRRDYFGLDVGLDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWSANKIVLGKLSGRNAFKQRLQELGIALDSEGELNTAFA
CCCCCEECCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCEECCCCCHHHHHH
RFKELADRKSEIFDEDIIAIVTEESAEAQEKEHYKFLSLSQHSETGEQPHAKIVFSVEGK
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHEEEECCCCCCCCCCCEEEEEEECCC
EVTGEARGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
EEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCEEEC
VGTDPDIVAASAKAYISALNKLYSNVDKLNPQRSE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDSIHT
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAGLIKDSTICSLARANDKDIQRAADALKPADRFRIHTFIATSPLHMEKKLRMTPDQVFE
HHHHHHCHHHHHHHCCCHHHHHHHHHHCCCCHHEEEEEEEECCCHHHHHHCCCCHHHHHH
QAKLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIAEGATTINIADTVGYGVPELY
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHH
GQLVKTLRERIPNSHKAVFSVHCHNDLGMAVANSLAGVQIGGARQVECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVKTRRDYFGLDVGLDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWSANKIVLGKLSGRNAFKQRLQELGIALDSEGELNTAFA
CCCCCEECCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCEECCCCCHHHHHH
RFKELADRKSEIFDEDIIAIVTEESAEAQEKEHYKFLSLSQHSETGEQPHAKIVFSVEGK
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHEEEECCCCCCCCCCCEEEEEEECCC
EVTGEARGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
EEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCEEEC
VGTDPDIVAASAKAYISALNKLYSNVDKLNPQRSE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA