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The map label for this gene is budB [H]

Identifier: 91782531

GI number: 91782531

Start: 1292990

End: 1294642

Strand: Direct

Name: budB [H]

Synonym: Bxe_A3298

Alternate gene names: 91782531

Gene position: 1292990-1294642 (Clockwise)

Preceding gene: 91782530

Following gene: 91782532

Centisome position: 26.41

GC content: 61.58

Gene sequence:

>1653_bases
ATGAAAGCATCGGACCTGTTCGTCAAATCGCTGGAAGCCGAAGGTGTCGAGTATGTGTTCGGCATTCCCGGTGAAGAAAA
CCTAGATCTGCTCGAATCGCTGCGCCGCTCGAAAATCCGTCTGATCCTCACGCGCCACGAACAGGCGGCCGGTTTCATGG
CCGCCACGTACGGGCGTCTTACCGGCCGCATCGGCGTATGTCTGGCCACGCTCGGCCCGGGCGCGACCAACCTCGTGACC
GCCGCGGCGTACGCGCAACTGGGCGGCATGCCGATGCTGATGGTCACCGGGCAAAAGCCGATCAAGTCGAGCAAGCAAGG
GCATTTCCAGATCGTCGACGTGGTGCGCATGATGGAGCCGCTCACCAAATACACGCGGCAGATCGTGTCGGTCGCCAATA
TTCCTGCGGCGGTGCGCGAGGCGGCCCGGCAGGCGGAAGAAGAACGGCCCGGCGCCACGCACCTCGAATTGCCTGAAGAC
GTGGCGCACGAAGAGGGCGATGGCAAGCCGATTCCGAAGAGCTACAGCCGCCGCCCCGTCGCCGAGGAAAAAGCCGTGGC
GCGCGCGGTCGAGGCGATCACCGGCGCGAAGCATCCGTTACTGATGATCGGCGCGGGCGGCAATCGCAAGACCACCACGA
AGATGCTGCGCGAGTTCGTCGACCAGATCGGCATTCCGTTTTTCACCACGCAGATGGGCAAAGGCGTGATCGACGAATCG
CATCCCATGTGGCTGGGCAATGCGACGCTGTCCGACGGCGACTTCGTGCACCGCGCGATCGACCACGCGGACTGCATCAT
CAATGTCGGTCACGACGTGATCGAGAAGCCGCCGTTTTTCATGCGCAGCGGCGACGCGGGCGAGAAGACGGTGATTCACG
TCAACTTCCTCGGCGCGGAAGTCGATACGGTCTACTTCCCGCAGATCGAAGTGGTGGGCGATATCGCCAACGCCGTGTGG
CAGCTCAAGGAAAGTCTCAAGCAGCGGCAGGAGCATTGGGACTTCACGCGCTTCAAGGAAATCAAGGAGCACTTCGAGGC
GCATCTGGTCAAAGGCCAGCACGACGACCGCTTTCCCATGTACCCGGTGCGGATCGTCAACGACGTGTACGAGACCACGC
CGGTGGACGGTATCGTGTGTCTGGACAACGGCATGTACAAGATCTGGTTCGCCCGCTACTACCGCGCGCACGAACCGAAT
TCGCTGCTGCTCGACAACGCGTTGGCGTCGATGGGCGCGGGTCTGCCGTCCGCGATCGCCACCAAGATCGTGCACCCGGA
CCGCAAGGTCATGGCCGTATGCGGCGACGGCGGTTTCATGATGAATTCGCAGGAACTCGAAACGGCGGTGCGGCTGAGGC
TGGATCTGGTGATCCTGATTTTGCGCGACGACGCGTTCGGCATGATCCGCTGGAAGCAGGAAAACATGAATTTCCCCGAC
TACGGCATGACGCTCAGCAATCCGGATTTCGTCGCTTACGCGCAGAGCTACGGGGCGAAAGGTCACCGTATCGAAACGGC
GGCGGAGTTCGCGCCGTTGCTGCGTGAATGTTTCGCGACGCCCGGCGTGCATGTGATCGACCTGCCAATCGACTATTCGG
ACAACGAACGCGTGCTGAATCGCGAGATCAAGCGGCTTTCGGCGCAATTGTGA

Upstream 100 bases:

>100_bases
CGGGCGGCGTCCGCGTGGCTGTCGTCGTCTTCGATCGTCCGTTTCCGGACCCCGGCGCCGGGGTTGCGGCCCGCGTCCGT
ATCATCAAGCGAGACCCTCA

Downstream 100 bases:

>100_bases
ACGGCAGATGTGTGGCAATGAACGGCCGCGGGGCATGGCCCCGGCTGGCAGTCCTGCTTCGGCAGGCATCGGATCAAGGA
GACGTGTCATGTTGCAAAAG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSKIRLILTRHEQAAGFMAATYGRLTGRIGVCLATLGPGATNLVT
AAAYAQLGGMPMLMVTGQKPIKSSKQGHFQIVDVVRMMEPLTKYTRQIVSVANIPAAVREAARQAEEERPGATHLELPED
VAHEEGDGKPIPKSYSRRPVAEEKAVARAVEAITGAKHPLLMIGAGGNRKTTTKMLREFVDQIGIPFFTTQMGKGVIDES
HPMWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRSGDAGEKTVIHVNFLGAEVDTVYFPQIEVVGDIANAVW
QLKESLKQRQEHWDFTRFKEIKEHFEAHLVKGQHDDRFPMYPVRIVNDVYETTPVDGIVCLDNGMYKIWFARYYRAHEPN
SLLLDNALASMGAGLPSAIATKIVHPDRKVMAVCGDGGFMMNSQELETAVRLRLDLVILILRDDAFGMIRWKQENMNFPD
YGMTLSNPDFVAYAQSYGAKGHRIETAAEFAPLLRECFATPGVHVIDLPIDYSDNERVLNREIKRLSAQL

Sequences:

>Translated_550_residues
MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSKIRLILTRHEQAAGFMAATYGRLTGRIGVCLATLGPGATNLVT
AAAYAQLGGMPMLMVTGQKPIKSSKQGHFQIVDVVRMMEPLTKYTRQIVSVANIPAAVREAARQAEEERPGATHLELPED
VAHEEGDGKPIPKSYSRRPVAEEKAVARAVEAITGAKHPLLMIGAGGNRKTTTKMLREFVDQIGIPFFTTQMGKGVIDES
HPMWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRSGDAGEKTVIHVNFLGAEVDTVYFPQIEVVGDIANAVW
QLKESLKQRQEHWDFTRFKEIKEHFEAHLVKGQHDDRFPMYPVRIVNDVYETTPVDGIVCLDNGMYKIWFARYYRAHEPN
SLLLDNALASMGAGLPSAIATKIVHPDRKVMAVCGDGGFMMNSQELETAVRLRLDLVILILRDDAFGMIRWKQENMNFPD
YGMTLSNPDFVAYAQSYGAKGHRIETAAEFAPLLRECFATPGVHVIDLPIDYSDNERVLNREIKRLSAQL
>Mature_550_residues
MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSKIRLILTRHEQAAGFMAATYGRLTGRIGVCLATLGPGATNLVT
AAAYAQLGGMPMLMVTGQKPIKSSKQGHFQIVDVVRMMEPLTKYTRQIVSVANIPAAVREAARQAEEERPGATHLELPED
VAHEEGDGKPIPKSYSRRPVAEEKAVARAVEAITGAKHPLLMIGAGGNRKTTTKMLREFVDQIGIPFFTTQMGKGVIDES
HPMWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRSGDAGEKTVIHVNFLGAEVDTVYFPQIEVVGDIANAVW
QLKESLKQRQEHWDFTRFKEIKEHFEAHLVKGQHDDRFPMYPVRIVNDVYETTPVDGIVCLDNGMYKIWFARYYRAHEPN
SLLLDNALASMGAGLPSAIATKIVHPDRKVMAVCGDGGFMMNSQELETAVRLRLDLVILILRDDAFGMIRWKQENMNFPD
YGMTLSNPDFVAYAQSYGAKGHRIETAAEFAPLLRECFATPGVHVIDLPIDYSDNERVLNREIKRLSAQL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=546, Percent_Identity=27.2893772893773, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI21361361, Length=539, Percent_Identity=26.9016697588126, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1790104, Length=539, Percent_Identity=29.4990723562152, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87081685, Length=535, Percent_Identity=27.4766355140187, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1786717, Length=539, Percent_Identity=25.2319109461967, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787096, Length=532, Percent_Identity=24.4360902255639, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788716, Length=474, Percent_Identity=26.1603375527426, Blast_Score=129, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17542570, Length=547, Percent_Identity=23.9488117001828, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=566, Percent_Identity=23.8515901060071, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=566, Percent_Identity=23.8515901060071, Blast_Score=135, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=557, Percent_Identity=29.802513464991, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=24.8459958932238, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321524, Length=277, Percent_Identity=28.158844765343, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=269, Percent_Identity=28.996282527881, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=277, Percent_Identity=27.7978339350181, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320123, Length=286, Percent_Identity=24.4755244755245, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=483, Percent_Identity=26.9151138716356, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61201; Mature: 61201

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSKIRLILTRHEQAAGFMAATYGRL
CCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHH
TGRIGVCLATLGPGATNLVTAAAYAQLGGMPMLMVTGQKPIKSSKQGHFQIVDVVRMMEP
HCCHHEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LTKYTRQIVSVANIPAAVREAARQAEEERPGATHLELPEDVAHEEGDGKPIPKSYSRRPV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
AEEKAVARAVEAITGAKHPLLMIGAGGNRKTTTKMLREFVDQIGIPFFTTQMGKGVIDES
HHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCC
HPMWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRSGDAGEKTVIHVNFLGAE
CCEEECCCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCEEECCCCCCEEEEEEEEECCC
VDTVYFPQIEVVGDIANAVWQLKESLKQRQEHWDFTRFKEIKEHFEAHLVKGQHDDRFPM
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCC
YPVRIVNDVYETTPVDGIVCLDNGMYKIWFARYYRAHEPNSLLLDNALASMGAGLPSAIA
CHHHHHHHHHHCCCCCEEEEECCCEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHH
TKIVHPDRKVMAVCGDGGFMMNSQELETAVRLRLDLVILILRDDAFGMIRWKQENMNFPD
HHHCCCCCEEEEEECCCCEEECHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCCCC
YGMTLSNPDFVAYAQSYGAKGHRIETAAEFAPLLRECFATPGVHVIDLPIDYSDNERVLN
CCCEECCCCCEEEHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHH
REIKRLSAQL
HHHHHHHCCC
>Mature Secondary Structure
MKASDLFVKSLEAEGVEYVFGIPGEENLDLLESLRRSKIRLILTRHEQAAGFMAATYGRL
CCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHH
TGRIGVCLATLGPGATNLVTAAAYAQLGGMPMLMVTGQKPIKSSKQGHFQIVDVVRMMEP
HCCHHEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LTKYTRQIVSVANIPAAVREAARQAEEERPGATHLELPEDVAHEEGDGKPIPKSYSRRPV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
AEEKAVARAVEAITGAKHPLLMIGAGGNRKTTTKMLREFVDQIGIPFFTTQMGKGVIDES
HHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCC
HPMWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRSGDAGEKTVIHVNFLGAE
CCEEECCCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCEEECCCCCCEEEEEEEEECCC
VDTVYFPQIEVVGDIANAVWQLKESLKQRQEHWDFTRFKEIKEHFEAHLVKGQHDDRFPM
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCC
YPVRIVNDVYETTPVDGIVCLDNGMYKIWFARYYRAHEPNSLLLDNALASMGAGLPSAIA
CHHHHHHHHHHCCCCCEEEEECCCEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHH
TKIVHPDRKVMAVCGDGGFMMNSQELETAVRLRLDLVILILRDDAFGMIRWKQENMNFPD
HHHCCCCCEEEEEECCCCEEECHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCCCC
YGMTLSNPDFVAYAQSYGAKGHRIETAAEFAPLLRECFATPGVHVIDLPIDYSDNERVLN
CCCEECCCCCEEEHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHH
REIKRLSAQL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]