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The map label for this gene is cobS [H]

Identifier: 91782333

GI number: 91782333

Start: 1064075

End: 1064851

Strand: Direct

Name: cobS [H]

Synonym: Bxe_A3498

Alternate gene names: 91782333

Gene position: 1064075-1064851 (Clockwise)

Preceding gene: 91782332

Following gene: 91782334

Centisome position: 21.73

GC content: 62.93

Gene sequence:

>777_bases
ATGAACCCGCTCACGGAACTGCGCTATTTCTTCACGGCGCTCGGCTATTTCACGCGCGTGCCCGTGCCACGCTGGGTCGG
TTATGAACCGCATTATCTGAATGCGGCGGCGCGTTATTTTCCGCTCGTGGGCGTATTGATCGGCGCATTGAGCGCGCTGG
TCTATCTCGCCGCGCTGCACGTCTTTCCGGCGGGCGTGGCGGTGCTGCTCTCGATGGCCGCGTCGCTGCTCGTGACCGGC
GCGTTCCACGAAGACGGTCTGGCGGATTGCGTCGATGCGTTCGGTGGCGCCTATACGCGCGAAGACGTATTGCGAATCAT
GCATGACTCGCGCATCGGTGCGTTCGGCGCCATCGCGCTGGTGATGGCCCTTGCATTGAAATGGCAAGCGCTCGCCGCCA
TGCCGCCGCTGCGCGCCGCAAGTCTGATGATTGCCGCGCATGCGGCGAGCCGAACGTGCGCGATCAGCTATCTGGCCACG
CTCGACTACGTGCGCGCCGAAGGCAAGGCCAAACCGGTGGCGCAGCGCCTGAGCGGCGGCGCATTCATCTGCGCCGCGAT
ATGCGGTTTGCCGTGGCTGTTCTGGCCGGACTGGTTCCATGCGCCTGACTGGCGCTTCGCGGGCTCGGCGCTTGCGGGTC
TAGTCGTACTTCGCGTGGCAATGGGCCGTTATTTCGTCAGACGCATCGGCGGTTATACCGGCGACTGCCTCGGTTTCGCG
CAGCAGATTTTTGAATTGAGCATTTATCTGGTGGGGCTGATATGGATATCGTCCTGA

Upstream 100 bases:

>100_bases
GTTCATCAACGAGATGGCGAGCTTCGAATCGGCCGGCGTCGCGAATCGCGATGCGTGATCCGCTGGATCCTTCAGACCGA
CCCGCACGCCGGGCACGCTC

Downstream 100 bases:

>100_bases
TTCGTCATCCCGCGGTCGCGCTCGATGCGGGCGTGTGTTACGGCCATAGCGACGTGGCGCTCGCTGACAATGCCGAGGTG
TCGTCGGCGGCGCTGGGCCT

Product: cobalamin synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNPLTELRYFFTALGYFTRVPVPRWVGYEPHYLNAAARYFPLVGVLIGALSALVYLAALHVFPAGVAVLLSMAASLLVTG
AFHEDGLADCVDAFGGAYTREDVLRIMHDSRIGAFGAIALVMALALKWQALAAMPPLRAASLMIAAHAASRTCAISYLAT
LDYVRAEGKAKPVAQRLSGGAFICAAICGLPWLFWPDWFHAPDWRFAGSALAGLVVLRVAMGRYFVRRIGGYTGDCLGFA
QQIFELSIYLVGLIWISS

Sequences:

>Translated_258_residues
MNPLTELRYFFTALGYFTRVPVPRWVGYEPHYLNAAARYFPLVGVLIGALSALVYLAALHVFPAGVAVLLSMAASLLVTG
AFHEDGLADCVDAFGGAYTREDVLRIMHDSRIGAFGAIALVMALALKWQALAAMPPLRAASLMIAAHAASRTCAISYLAT
LDYVRAEGKAKPVAQRLSGGAFICAAICGLPWLFWPDWFHAPDWRFAGSALAGLVVLRVAMGRYFVRRIGGYTGDCLGFA
QQIFELSIYLVGLIWISS
>Mature_258_residues
MNPLTELRYFFTALGYFTRVPVPRWVGYEPHYLNAAARYFPLVGVLIGALSALVYLAALHVFPAGVAVLLSMAASLLVTG
AFHEDGLADCVDAFGGAYTREDVLRIMHDSRIGAFGAIALVMALALKWQALAAMPPLRAASLMIAAHAASRTCAISYLAT
LDYVRAEGKAKPVAQRLSGGAFICAAICGLPWLFWPDWFHAPDWRFAGSALAGLVVLRVAMGRYFVRRIGGYTGDCLGFA
QQIFELSIYLVGLIWISS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=255, Percent_Identity=34.1176470588235, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 27939; Mature: 27939

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA