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The map label for this gene is thcD [H]

Identifier: 91782244

GI number: 91782244

Start: 965211

End: 966467

Strand: Reverse

Name: thcD [H]

Synonym: Bxe_A3591

Alternate gene names: 91782244

Gene position: 966467-965211 (Counterclockwise)

Preceding gene: 91782245

Following gene: 91782243

Centisome position: 19.74

GC content: 63.72

Gene sequence:

>1257_bases
ATGTCCGATCTGTCTTCACACATCCTCGTGCTAGGTGCTGGCCACGCCGGCGACTCGGTAGCCGCCTTTTTACGGCAATA
CGGCTGGCAGGGCTCGATTACGCTCGCTGGCAATGAAGGCACCGCGCCCTATCATCGCCCGCCCCTGTCCAAGGCGCTGC
TGCTCGGTCAGATTCCTGACGACGATCTGTCCCTGCGTCCCGCGTCGTTCTACGACAAACAGAACATCCGGTTGCTCACC
GACATACACGCAATCCAGATCGAGCGCGCCCAGCGCCGCGTCAAGGTCCGCAACGGTGAGCCGCTTGCCTATGACCACCT
GATTCTTGCGACCGGAGCCAGGGTGCGCGCGCTCGATGTCCCCGGCAAAGAATTAGAAGGCGTCATGTCATTACGGGGAC
GCGCCGATTCGCTCGCCTTTCGCGACGCCATTCGTCCCCGCAAACACGTGGTCATCGTCGGCGGCGGTTATATCGGTCTC
GAAGCGGCCGCGTCGGCGTCGAAACTCGGTGCAAACGTTACGGTGATCGAGCGCGAAGCGCGTCTGCTTGCACGGGTGGC
GTCGCCGTTTCTCGCACAGTATGTACACGATTACTACCGCGCACGAGATGTAGCAATCGAGTTGAGCGCTTCGGTCGCGG
GCTTCGAAGGCAACGGCGGTCGCGTCACCGAGGTGCGGCTCGCCGATGACCGGACGTTGCTGTGCGATGTCGCGCTGGTC
GGCATCGGCGTGCTCGCAGAGCAATCGCTCGCGGTGGCTGCGGGCCTCGCGTGCAACGACGGCGTCCTGGTCGACGAACA
CGCGCGCACCAGCGATGAGCGTATTCATGCAATCGGCGATTGCACCCGGCGTCCGCTAAGGCTCGGCGGTTCGGATCTGA
TGGTGCGACTCGAAAGTGTACACAACGCAACCGAGCAGGCGCGACAGGCCGCGTCCGATCTTTGCGGCCGCGCGGCACCG
GTTGTCGAGTGTCCGTGGACGTGGTCGGATCAGTTCGATCTACGCCTTCAACTGGCCGGCTTTCCGAAGGCTGCGGCCGA
CACCGTGGTGCGCGGCGATCCGATGAAAGACGCCTTCGCCATATTCCATCTGGATGCCGCAGCGCGACTTCAGGCCGTCG
AGGCGATCAATGCACCTACCGAATTTGCGTTCGCGCGCCGCGTGGCAGCGAGCGGCGTGCCTTTACCGCGCAACCGGCTG
AGCGACCCCGCTGTGCCGCTGAAAGCCCTCATGGATGACGCACTCACACGCGCCTGA

Upstream 100 bases:

>100_bases
GTACCGGCACGGAAAAGCGACCGGCGCGCTGCCCGGAAAACTCGTGCGCGGCCAGCAACCCGCGCCGCAATGCCAGTAAA
CCACCGGGACGACTTCACTT

Downstream 100 bases:

>100_bases
TCTCATCCACACGAATATCGGATTGCCCGAGAACACTACACGATTTCACAAGGAGACAGCCATGCCAACTATTCATTTCA
TCACATACAACGGCGAAACA

Product: putative redutase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSDLSSHILVLGAGHAGDSVAAFLRQYGWQGSITLAGNEGTAPYHRPPLSKALLLGQIPDDDLSLRPASFYDKQNIRLLT
DIHAIQIERAQRRVKVRNGEPLAYDHLILATGARVRALDVPGKELEGVMSLRGRADSLAFRDAIRPRKHVVIVGGGYIGL
EAAASASKLGANVTVIEREARLLARVASPFLAQYVHDYYRARDVAIELSASVAGFEGNGGRVTEVRLADDRTLLCDVALV
GIGVLAEQSLAVAAGLACNDGVLVDEHARTSDERIHAIGDCTRRPLRLGGSDLMVRLESVHNATEQARQAASDLCGRAAP
VVECPWTWSDQFDLRLQLAGFPKAAADTVVRGDPMKDAFAIFHLDAAARLQAVEAINAPTEFAFARRVAASGVPLPRNRL
SDPAVPLKALMDDALTRA

Sequences:

>Translated_418_residues
MSDLSSHILVLGAGHAGDSVAAFLRQYGWQGSITLAGNEGTAPYHRPPLSKALLLGQIPDDDLSLRPASFYDKQNIRLLT
DIHAIQIERAQRRVKVRNGEPLAYDHLILATGARVRALDVPGKELEGVMSLRGRADSLAFRDAIRPRKHVVIVGGGYIGL
EAAASASKLGANVTVIEREARLLARVASPFLAQYVHDYYRARDVAIELSASVAGFEGNGGRVTEVRLADDRTLLCDVALV
GIGVLAEQSLAVAAGLACNDGVLVDEHARTSDERIHAIGDCTRRPLRLGGSDLMVRLESVHNATEQARQAASDLCGRAAP
VVECPWTWSDQFDLRLQLAGFPKAAADTVVRGDPMKDAFAIFHLDAAARLQAVEAINAPTEFAFARRVAASGVPLPRNRL
SDPAVPLKALMDDALTRA
>Mature_417_residues
SDLSSHILVLGAGHAGDSVAAFLRQYGWQGSITLAGNEGTAPYHRPPLSKALLLGQIPDDDLSLRPASFYDKQNIRLLTD
IHAIQIERAQRRVKVRNGEPLAYDHLILATGARVRALDVPGKELEGVMSLRGRADSLAFRDAIRPRKHVVIVGGGYIGLE
AAASASKLGANVTVIEREARLLARVASPFLAQYVHDYYRARDVAIELSASVAGFEGNGGRVTEVRLADDRTLLCDVALVG
IGVLAEQSLAVAAGLACNDGVLVDEHARTSDERIHAIGDCTRRPLRLGGSDLMVRLESVHNATEQARQAASDLCGRAAPV
VECPWTWSDQFDLRLQLAGFPKAAADTVVRGDPMKDAFAIFHLDAAARLQAVEAINAPTEFAFARRVAASGVPLPRNRLS
DPAVPLKALMDDALTRA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=361, Percent_Identity=27.9778393351801, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI226437568, Length=361, Percent_Identity=27.9778393351801, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI21389617, Length=361, Percent_Identity=27.9778393351801, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4757732, Length=325, Percent_Identity=25.2307692307692, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI22202629, Length=325, Percent_Identity=25.2307692307692, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788892, Length=403, Percent_Identity=34.4913151364764, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789065, Length=278, Percent_Identity=30.2158273381295, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1789765, Length=262, Percent_Identity=29.3893129770992, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=362, Percent_Identity=29.0055248618785, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24639257, Length=400, Percent_Identity=26.75, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281359715, Length=401, Percent_Identity=26.6832917705736, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281359713, Length=401, Percent_Identity=26.6832917705736, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24639250, Length=401, Percent_Identity=26.6832917705736, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI18543267, Length=401, Percent_Identity=26.6832917705736, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24639252, Length=401, Percent_Identity=26.6832917705736, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24585130, Length=400, Percent_Identity=23.25, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581020, Length=368, Percent_Identity=24.7282608695652, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28573993, Length=368, Percent_Identity=24.7282608695652, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 44859; Mature: 44727

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLSSHILVLGAGHAGDSVAAFLRQYGWQGSITLAGNEGTAPYHRPPLSKALLLGQIPD
CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHEECCCCC
DDLSLRPASFYDKQNIRLLTDIHAIQIERAQRRVKVRNGEPLAYDHLILATGARVRALDV
CCCCCCCCHHCCCCCCEEEEHHHHHHHHHHHHHEEECCCCCCEECEEEEECCCEEEEECC
PGKELEGVMSLRGRADSLAFRDAIRPRKHVVIVGGGYIGLEAAASASKLGANVTVIEREA
CCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCEECHHHHCCHHHCCCCEEEEHHHH
RLLARVASPFLAQYVHDYYRARDVAIELSASVAGFEGNGGRVTEVRLADDRTLLCDVALV
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECEEECEECCCCEEEEEEECCCCEEEHHHHHH
GIGVLAEQSLAVAAGLACNDGVLVDEHARTSDERIHAIGDCTRRPLRLGGSDLMVRLESV
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHCCCHHHEEEEHHH
HNATEQARQAASDLCGRAAPVVECPWTWSDQFDLRLQLAGFPKAAADTVVRGDPMKDAFA
HHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEEEECCCCCHHHCCEECCCCCCCEEE
IFHLDAAARLQAVEAINAPTEFAFARRVAASGVPLPRNRLSDPAVPLKALMDDALTRA
EEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDLSSHILVLGAGHAGDSVAAFLRQYGWQGSITLAGNEGTAPYHRPPLSKALLLGQIPD
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHEECCCCC
DDLSLRPASFYDKQNIRLLTDIHAIQIERAQRRVKVRNGEPLAYDHLILATGARVRALDV
CCCCCCCCHHCCCCCCEEEEHHHHHHHHHHHHHEEECCCCCCEECEEEEECCCEEEEECC
PGKELEGVMSLRGRADSLAFRDAIRPRKHVVIVGGGYIGLEAAASASKLGANVTVIEREA
CCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCEECHHHHCCHHHCCCCEEEEHHHH
RLLARVASPFLAQYVHDYYRARDVAIELSASVAGFEGNGGRVTEVRLADDRTLLCDVALV
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECEEECEECCCCEEEEEEECCCCEEEHHHHHH
GIGVLAEQSLAVAAGLACNDGVLVDEHARTSDERIHAIGDCTRRPLRLGGSDLMVRLESV
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHCCCHHHEEEEHHH
HNATEQARQAASDLCGRAAPVVECPWTWSDQFDLRLQLAGFPKAAADTVVRGDPMKDAFA
HHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEEEECCCCCHHHCCEECCCCCCCEEE
IFHLDAAARLQAVEAINAPTEFAFARRVAASGVPLPRNRLSDPAVPLKALMDDALTRA
EEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]