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The map label for this gene is ilvD2 [H]

Identifier: 91782200

GI number: 91782200

Start: 923594

End: 925267

Strand: Reverse

Name: ilvD2 [H]

Synonym: Bxe_A3634

Alternate gene names: 91782200

Gene position: 925267-923594 (Counterclockwise)

Preceding gene: 91782203

Following gene: 91782199

Centisome position: 18.9

GC content: 64.4

Gene sequence:

>1674_bases
ATGCCATACAACCGTCGTTCGAAGAACATCACGCAAGGTGTAGCGCGTTCGCCGAATCGCTCGATGTACTACGCGCTCGG
CTACGAAAAAGCCGACTTCGACAAGCCGATGATCGGCATCGCCAACGGTCACTCGACCATCACGCCGTGCAACGCGGGCC
TGCAGCGTCTCGCCGATGCCGCGGTCGCCGCGATCAAAGGCGCCGACGCGAACCCACAGATCTTCGGCACGCCGACGATC
TCGGACGGCATGTCGATGGGCACCGAGGGCATGAAGTACTCGCTGGTGTCGCGCGAAGTGATTTCCGACTGCATCGAGAC
CTGCGTGCAAGGCCAGTGGATGGACGGCGTGGTCGTGATCGGCGGCTGCGACAAGAACATGCCGGGCGGCATGATCGGCA
TGCTGCGCACCAACGTGCCGAGCATCTACGTGTATGGCGGGACGATTCGTCCGGGCAACTGGAAGGGCACCGACCTGACC
ATCGTGTCGTCGTTCGAGGCCGTGGGCGAATTCACCGCCGGCCGGATGTCGCAGGAAGATTTCGAAGGGGTCGAACAGAA
CGCGTGCCCATCCACCGGGTCGTGCGGCGGCATGTACACCGCCAACACCATGAGCTCGTCGTTCGAAGCGCTCGGCATGT
CGCTGCTGTATTCGTCCACAATGGCCAATCCGGATCAGGAAAAAGTCGACTCGGCCGCCGAATCGGCACGCGTGCTGGTC
GAAGCGGTCAAGCAGGATCTCAAGCCGCGCGACATCGTGACCCGCAAGTCGATCGAAAACGCCGTGGCCGTGATCATGGC
CACCGGCGGCTCGACCAATGCCGTGCTGCACTTTCTCGCCATCGCGCATGCGGCCGAAGTGGAGTGGAGCATCGAGGACT
TCGAGCGCATGCGCAAGAAGGTGCCGGTGATCTGCAACCTGAAGCCGTCGGGCCAGTTCGTGGCGACCGATCTGCACAAG
GCCGGCGGCATTCCGCAAGTCATGAAGATCCTGCTCGACGCAGGTCTGTTGCACGGCGATTGCATCACGATCACCGGCCG
AACCCTCGCGGAAGAGTTGAAGGACGTGCCGGCCAGGCCGCGCGCCGATCAGCAGGTGATTTTCCCGATCGAGCAGGCGC
TGTACAAGGAAGGCCATCTCGCGATCCTCAAGGGCAACCTGGCCGTGGACGGCGCGGTCGCCAAGATCACCGGCCTGAAG
AATCCCGTGATCACCGGCCCGGCGCGCGTGTTCGACGACGAGCAAAGCGCGCTGGAAGCGATTCTGGCCGACAGGATCGT
CGCGGGCGACGTGGTGGTGCTGCGCTATCTCGGCCCGAAGGGCGGCCCCGGCATGCCGGAGATGCTCGCGCCGACCTCGG
CGATCATCGGCAAGGGACTCGGCGAGAGCGTGGGGCTCATCACCGACGGGCGCTTCTCGGGTGGCACGTGGGGCATGGTG
GTCGGGCACGTCGCGCCGGAAGCCTTCGTGGGCGGCACGATCGCGTTCGTGCAGGAAGGCGACTCGATCACCATCGACGC
GCACAAGCTGCTGCTGCAACTGAATATCGACGACGCCGAACTGGAGCGACGCCGTGCCGCATGGCAGCAGCCGAAGCCGC
GCTACACACGCGGCGTGCTGGCGAAGTACTCCGCATTGGCTCTGCCGGCCAATAAGGGCGCCGTGACGGGCTAA

Upstream 100 bases:

>100_bases
GCGGCGCGCCGGAACTACACTGAACGGATGCGGCCAGGCCACCCACGCCGGCACGGCAGGACCCGCAGCGCGGACCAAAA
CAGAACAGACTGGAGCTCCC

Downstream 100 bases:

>100_bases
AGCTGCGTTCAGCGGATACGTCAGCGGCCCTGCGAGTACGCGCCTGGCTACGTAAAAGCGAAAGGGGCGGACGGGAAACC
GTGCGCCCCTTTCCTTTTAT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 2 [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MPYNRRSKNITQGVARSPNRSMYYALGYEKADFDKPMIGIANGHSTITPCNAGLQRLADAAVAAIKGADANPQIFGTPTI
SDGMSMGTEGMKYSLVSREVISDCIETCVQGQWMDGVVVIGGCDKNMPGGMIGMLRTNVPSIYVYGGTIRPGNWKGTDLT
IVSSFEAVGEFTAGRMSQEDFEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
EAVKQDLKPRDIVTRKSIENAVAVIMATGGSTNAVLHFLAIAHAAEVEWSIEDFERMRKKVPVICNLKPSGQFVATDLHK
AGGIPQVMKILLDAGLLHGDCITITGRTLAEELKDVPARPRADQQVIFPIEQALYKEGHLAILKGNLAVDGAVAKITGLK
NPVITGPARVFDDEQSALEAILADRIVAGDVVVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMV
VGHVAPEAFVGGTIAFVQEGDSITIDAHKLLLQLNIDDAELERRRAAWQQPKPRYTRGVLAKYSALALPANKGAVTG

Sequences:

>Translated_557_residues
MPYNRRSKNITQGVARSPNRSMYYALGYEKADFDKPMIGIANGHSTITPCNAGLQRLADAAVAAIKGADANPQIFGTPTI
SDGMSMGTEGMKYSLVSREVISDCIETCVQGQWMDGVVVIGGCDKNMPGGMIGMLRTNVPSIYVYGGTIRPGNWKGTDLT
IVSSFEAVGEFTAGRMSQEDFEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
EAVKQDLKPRDIVTRKSIENAVAVIMATGGSTNAVLHFLAIAHAAEVEWSIEDFERMRKKVPVICNLKPSGQFVATDLHK
AGGIPQVMKILLDAGLLHGDCITITGRTLAEELKDVPARPRADQQVIFPIEQALYKEGHLAILKGNLAVDGAVAKITGLK
NPVITGPARVFDDEQSALEAILADRIVAGDVVVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMV
VGHVAPEAFVGGTIAFVQEGDSITIDAHKLLLQLNIDDAELERRRAAWQQPKPRYTRGVLAKYSALALPANKGAVTG
>Mature_556_residues
PYNRRSKNITQGVARSPNRSMYYALGYEKADFDKPMIGIANGHSTITPCNAGLQRLADAAVAAIKGADANPQIFGTPTIS
DGMSMGTEGMKYSLVSREVISDCIETCVQGQWMDGVVVIGGCDKNMPGGMIGMLRTNVPSIYVYGGTIRPGNWKGTDLTI
VSSFEAVGEFTAGRMSQEDFEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLVE
AVKQDLKPRDIVTRKSIENAVAVIMATGGSTNAVLHFLAIAHAAEVEWSIEDFERMRKKVPVICNLKPSGQFVATDLHKA
GGIPQVMKILLDAGLLHGDCITITGRTLAEELKDVPARPRADQQVIFPIEQALYKEGHLAILKGNLAVDGAVAKITGLKN
PVITGPARVFDDEQSALEAILADRIVAGDVVVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMVV
GHVAPEAFVGGTIAFVQEGDSITIDAHKLLLQLNIDDAELERRRAAWQQPKPRYTRGVLAKYSALALPANKGAVTG

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=604, Percent_Identity=37.4172185430464, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1786464, Length=470, Percent_Identity=34.8936170212766, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI2367371, Length=490, Percent_Identity=33.8775510204082, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788157, Length=484, Percent_Identity=31.6115702479339, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6322476, Length=560, Percent_Identity=44.4642857142857, Blast_Score=485, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59134; Mature: 59002

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYNRRSKNITQGVARSPNRSMYYALGYEKADFDKPMIGIANGHSTITPCNAGLQRLADA
CCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
AVAAIKGADANPQIFGTPTISDGMSMGTEGMKYSLVSREVISDCIETCVQGQWMDGVVVI
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIGMLRTNVPSIYVYGGTIRPGNWKGTDLTIVSSFEAVGEFTAGRMSQED
ECCCCCCCCCEEEHEECCCCEEEEECCEECCCCCCCCCEEEEECHHHHHHHHHCCCCHHH
FEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
HHCHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAVKQDLKPRDIVTRKSIENAVAVIMATGGSTNAVLHFLAIAHAAEVEWSIEDFERMRKK
HHHHHCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHCEECHHHHHHHHHC
VPVICNLKPSGQFVATDLHKAGGIPQVMKILLDAGLLHGDCITITGRTLAEELKDVPARP
CCEEEEECCCCCEEEEHHHHHCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCC
RADQQVIFPIEQALYKEGHLAILKGNLAVDGAVAKITGLKNPVITGPARVFDDEQSALEA
CCCCEEEEHHHHHHHCCCCEEEEECCEEECCHHHEECCCCCCCEECCHHHCCCHHHHHHH
ILADRIVAGDVVVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMV
HHHHHHHHCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCHHEE
VGHVAPEAFVGGTIAFVQEGDSITIDAHKLLLQLNIDDAELERRRAAWQQPKPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHH
AKYSALALPANKGAVTG
HHHHHEEECCCCCCCCC
>Mature Secondary Structure 
PYNRRSKNITQGVARSPNRSMYYALGYEKADFDKPMIGIANGHSTITPCNAGLQRLADA
CCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
AVAAIKGADANPQIFGTPTISDGMSMGTEGMKYSLVSREVISDCIETCVQGQWMDGVVVI
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIGMLRTNVPSIYVYGGTIRPGNWKGTDLTIVSSFEAVGEFTAGRMSQED
ECCCCCCCCCEEEHEECCCCEEEEECCEECCCCCCCCCEEEEECHHHHHHHHHCCCCHHH
FEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
HHCHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAVKQDLKPRDIVTRKSIENAVAVIMATGGSTNAVLHFLAIAHAAEVEWSIEDFERMRKK
HHHHHCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHCEECHHHHHHHHHC
VPVICNLKPSGQFVATDLHKAGGIPQVMKILLDAGLLHGDCITITGRTLAEELKDVPARP
CCEEEEECCCCCEEEEHHHHHCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCC
RADQQVIFPIEQALYKEGHLAILKGNLAVDGAVAKITGLKNPVITGPARVFDDEQSALEA
CCCCEEEEHHHHHHHCCCCEEEEECCEEECCHHHEECCCCCCCEECCHHHCCCHHHHHHH
ILADRIVAGDVVVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMV
HHHHHHHHCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCHHEE
VGHVAPEAFVGGTIAFVQEGDSITIDAHKLLLQLNIDDAELERRRAAWQQPKPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHH
AKYSALALPANKGAVTG
HHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA