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The map label for this gene is cysB [H]

Identifier: 91782169

GI number: 91782169

Start: 884538

End: 885479

Strand: Reverse

Name: cysB [H]

Synonym: Bxe_A3665

Alternate gene names: 91782169

Gene position: 885479-884538 (Counterclockwise)

Preceding gene: 91782185

Following gene: 91782168

Centisome position: 18.09

GC content: 60.4

Gene sequence:

>942_bases
ATGAACCTGCATCAATTCCGCTTCGTCCGCGAGGCTGTGCGGCAGAATTTCAACCTGACGGAAGCGGCGAAAGCCCTGTT
TACAAGCCAGCCGGGCGTTTCCAAGGCGATCATCGAGCTGGAGGACGAACTCGGCGTGGAAATCTTCACACGACACGGCA
AGCGGGTGCGCTCGCTGACCGAGCCGGGGCGCATCATTCTTCAATCGGTTGAGAAGATTCTGCAGGAAGTCGAGAGCCTC
AAACGGGTCGGTAAAGATTACGCGGCGCAGGATCAGGGCAATCTGGTGATCGCCGCCACCCACACCCAGGCGCGCTACTC
GCTGCCCGCTGCGATCGCCGAATTCAAGAAGCGCTTTCCCAAGGTCCACCTTTCGATTCTGCAAGGCAGCCCGACTCAGG
TCGCCGAAATGGTGATCCACGACCAGGCCGACCTCTGCATCGCCACCGAGGCGATCGCCAACTATAAGGAACTGGTGTCG
CTGCCGTGCTTCCAGTGGCACCACGTGGCGGTCATGCAACCGGATCATCCATTGCTCGACCGCAAACTTTTGTCGCTGGA
TGATCTGACCCAGTATCCGCTGATCACCTACGACAACGCGTTCGCCGGCCGCACCAAGATCAACGAGGCGTTCCGCCTGC
GCGGTCTGCATCCGGACATCGTACTGGAGGCGATCGACGCCGACGTGATCAAGACCTACGTGGAACTGGGTCTGGGCGTC
GGCATCATGGCGGATATCGCGTTCAACGCCGAGCGCGACCGTCATCTGCGCGCCATGCCGGTCGGCCACCTGTTCGGCAG
CAACGTGACGCGGGTCGCGCTGAAACAGGGCGCCTATTTGCGCAGCTATGTGTACACGCTCGTCGAACTGCTGTCGCCGA
GCATGAACCGCAAGCTGATCGAGCAGGCGCTCAAGGGCGAACACGAAACCTACGAACTTTGA

Upstream 100 bases:

>100_bases
AATACAGATATCCATATTGAAAAAGATGGACAGATCGTAATAAACTCGCCTTATATTTCAAACGACTAAAAAATTCTTTT
GATATGCGGAGAGGTTATAT

Downstream 100 bases:

>100_bases
TATCACCACGCTTTCTCGCTACGGAGACCCATCGATGACGTCGTATAAGAAAAGAATCAGCGGCCTCGCCGCCGCTTTTG
GTTTGAATGCGCTCCTGCTC

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNLHQFRFVREAVRQNFNLTEAAKALFTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLTEPGRIILQSVEKILQEVESL
KRVGKDYAAQDQGNLVIAATHTQARYSLPAAIAEFKKRFPKVHLSILQGSPTQVAEMVIHDQADLCIATEAIANYKELVS
LPCFQWHHVAVMQPDHPLLDRKLLSLDDLTQYPLITYDNAFAGRTKINEAFRLRGLHPDIVLEAIDADVIKTYVELGLGV
GIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKQGAYLRSYVYTLVELLSPSMNRKLIEQALKGEHETYEL

Sequences:

>Translated_313_residues
MNLHQFRFVREAVRQNFNLTEAAKALFTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLTEPGRIILQSVEKILQEVESL
KRVGKDYAAQDQGNLVIAATHTQARYSLPAAIAEFKKRFPKVHLSILQGSPTQVAEMVIHDQADLCIATEAIANYKELVS
LPCFQWHHVAVMQPDHPLLDRKLLSLDDLTQYPLITYDNAFAGRTKINEAFRLRGLHPDIVLEAIDADVIKTYVELGLGV
GIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKQGAYLRSYVYTLVELLSPSMNRKLIEQALKGEHETYEL
>Mature_313_residues
MNLHQFRFVREAVRQNFNLTEAAKALFTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLTEPGRIILQSVEKILQEVESL
KRVGKDYAAQDQGNLVIAATHTQARYSLPAAIAEFKKRFPKVHLSILQGSPTQVAEMVIHDQADLCIATEAIANYKELVS
LPCFQWHHVAVMQPDHPLLDRKLLSLDDLTQYPLITYDNAFAGRTKINEAFRLRGLHPDIVLEAIDADVIKTYVELGLGV
GIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKQGAYLRSYVYTLVELLSPSMNRKLIEQALKGEHETYEL

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=304, Percent_Identity=43.0921052631579, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1788296, Length=296, Percent_Identity=44.9324324324324, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI157672245, Length=213, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI145693105, Length=245, Percent_Identity=25.7142857142857, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790399, Length=308, Percent_Identity=23.0519480519481, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788481, Length=248, Percent_Identity=27.8225806451613, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=27.7551020408163, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789204, Length=236, Percent_Identity=25.8474576271186, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=193, Percent_Identity=26.9430051813472, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35263; Mature: 35263

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHQFRFVREAVRQNFNLTEAAKALFTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCHHHHHHC
EPGRIILQSVEKILQEVESLKRVGKDYAAQDQGNLVIAATHTQARYSLPAAIAEFKKRFP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
KVHLSILQGSPTQVAEMVIHDQADLCIATEAIANYKELVSLPCFQWHHVAVMQPDHPLLD
CEEEHEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHH
RKLLSLDDLTQYPLITYDNAFAGRTKINEAFRLRGLHPDIVLEAIDADVIKTYVELGLGV
HHHHCHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
GIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKQGAYLRSYVYTLVELLSPSMNRKLI
HHHHHHHHCCCCCCCEECCCHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHH
EQALKGEHETYEL
HHHHCCCCCCCCC
>Mature Secondary Structure
MNLHQFRFVREAVRQNFNLTEAAKALFTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCHHHHHHC
EPGRIILQSVEKILQEVESLKRVGKDYAAQDQGNLVIAATHTQARYSLPAAIAEFKKRFP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
KVHLSILQGSPTQVAEMVIHDQADLCIATEAIANYKELVSLPCFQWHHVAVMQPDHPLLD
CEEEHEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHH
RKLLSLDDLTQYPLITYDNAFAGRTKINEAFRLRGLHPDIVLEAIDADVIKTYVELGLGV
HHHHCHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
GIMADIAFNAERDRHLRAMPVGHLFGSNVTRVALKQGAYLRSYVYTLVELLSPSMNRKLI
HHHHHHHHCCCCCCCEECCCHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHH
EQALKGEHETYEL
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]