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The map label for this gene is braC [H]

Identifier: 91782129

GI number: 91782129

Start: 840667

End: 841884

Strand: Reverse

Name: braC [H]

Synonym: Bxe_A3706

Alternate gene names: 91782129

Gene position: 841884-840667 (Counterclockwise)

Preceding gene: 91782130

Following gene: 91782128

Centisome position: 17.2

GC content: 61.74

Gene sequence:

>1218_bases
ATGGGAATTCGCTTGAAAAGCGTTGTGGGTGCGCTCGCGCTTTGTGCCCTCGCCAATGTCGGCTATGCGGCGGATCCGAT
CAAGATCGGCGTTGACGGACCTTTCACCGGCGGCTCGTCTTCGATGGGCGTGAGCATGCGAGACGGCGTGCGTCTCGCCA
CTGCCGAGATCAATAAGGCCGGCGGCGTGCTGGGCCGCCAGATCGTGCTGGTGGAGCGCGACGACGAAGCGAAGAACGAA
CGCGGCGTGCAGATTGCGCAAGAGCTGATCAATAAGGAAAAAGTCGTGGCGGTGGTCGGCTACATCAACACCGGCGTCGC
CCTCGCCTCGCAGCGCTTCTTCCAGGAAGCGAAGATTCCGGTCTTCAACAACGTCGCCACAGGCAGCGTCGTGACGAAGC
AGTTCACCGATCAGCCCGATAACTACGTGTTCCGCAATGCCGCCGCGGACCGCATCCAGGCGCCCATGATCGTCGAAGAA
GCCGTGACCAGACGCGGTTTCAGGAAGGTCGCGATTCTCGCCGACTCGACCAACTATGGCCAGCTCGGCCGCGAAGACCT
CGAAAAGGCGCTTGCCGCCAAAGGCGTAAAACCTGTCGCCGTCGAAAAATTCAATATCAAGGACGTCGACATGACCGCGC
AGCTGCTCAAGGCCAAAGAGGCCGGCGCGGAAGCGGTGCTGACATATGGCATTGGACCGGAACTCGCTCAGATCGCCAAC
GGCATGGCCAAGCTCGGCTGGAAAGTGCCGCTGATCGGCAGCTGGACTCTTTCGATGGCGAACTACATCGACAACGCGAG
CACCAACGGCGAAGGCGCGCGCATGCCGCAGACCTTCATTCAGGAAGCGAACACGCCGAAACGCAAAGCCTTCATCGACG
CATATCTGAAGGAATTCAAGCCGAAGAACAACCGCATCGACTCACCGGTTTCCGCCGCGCAAGGCTACGACTCGATCTAT
CTGCTGGCCGCCGCGATCACCCAGGCCGGCTCGACCGACGGCCCGAAGGTGCGCGCCGCGCTCGAAAACCTCAATACGAA
AGTGGAAGGCGTCGTGATGGTCTACGACAAGCCGTTCACCCACGACGACCACGAAGCGATCAGCCCGAACGTGCCGGTGG
TCGGCGAGGTCAAGGGCGGCCGCGTGGTCTACGCGTACGAGGCCGACCGCAAAGGCGGCGGCCAGTTGCGCACCAAGCAG
GCGAGCGCCGCGAATTGA

Upstream 100 bases:

>100_bases
CTCACCGGTTGACGGTCAGGCTTCCTAAGAACGTTCGAATCGGCGCGCACGGGCCTGGTTCGAGGCTTCGTGCAAGAAAA
GACATGCTACGGAGACAGTC

Downstream 100 bases:

>100_bases
GCTCGAAATAAGCGCAGCAGCAGCATGTTACGGAGCCGCGCCCGCCTGAAGGCGGGCGCGGCTTTGCTTCATATCCGGTG
AATTCGAGGGCGCCCGGCGA

Product: branched chain amino acid ABC transporter periplasmic ligand-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MGIRLKSVVGALALCALANVGYAADPIKIGVDGPFTGGSSSMGVSMRDGVRLATAEINKAGGVLGRQIVLVERDDEAKNE
RGVQIAQELINKEKVVAVVGYINTGVALASQRFFQEAKIPVFNNVATGSVVTKQFTDQPDNYVFRNAAADRIQAPMIVEE
AVTRRGFRKVAILADSTNYGQLGREDLEKALAAKGVKPVAVEKFNIKDVDMTAQLLKAKEAGAEAVLTYGIGPELAQIAN
GMAKLGWKVPLIGSWTLSMANYIDNASTNGEGARMPQTFIQEANTPKRKAFIDAYLKEFKPKNNRIDSPVSAAQGYDSIY
LLAAAITQAGSTDGPKVRAALENLNTKVEGVVMVYDKPFTHDDHEAISPNVPVVGEVKGGRVVYAYEADRKGGGQLRTKQ
ASAAN

Sequences:

>Translated_405_residues
MGIRLKSVVGALALCALANVGYAADPIKIGVDGPFTGGSSSMGVSMRDGVRLATAEINKAGGVLGRQIVLVERDDEAKNE
RGVQIAQELINKEKVVAVVGYINTGVALASQRFFQEAKIPVFNNVATGSVVTKQFTDQPDNYVFRNAAADRIQAPMIVEE
AVTRRGFRKVAILADSTNYGQLGREDLEKALAAKGVKPVAVEKFNIKDVDMTAQLLKAKEAGAEAVLTYGIGPELAQIAN
GMAKLGWKVPLIGSWTLSMANYIDNASTNGEGARMPQTFIQEANTPKRKAFIDAYLKEFKPKNNRIDSPVSAAQGYDSIY
LLAAAITQAGSTDGPKVRAALENLNTKVEGVVMVYDKPFTHDDHEAISPNVPVVGEVKGGRVVYAYEADRKGGGQLRTKQ
ASAAN
>Mature_404_residues
GIRLKSVVGALALCALANVGYAADPIKIGVDGPFTGGSSSMGVSMRDGVRLATAEINKAGGVLGRQIVLVERDDEAKNER
GVQIAQELINKEKVVAVVGYINTGVALASQRFFQEAKIPVFNNVATGSVVTKQFTDQPDNYVFRNAAADRIQAPMIVEEA
VTRRGFRKVAILADSTNYGQLGREDLEKALAAKGVKPVAVEKFNIKDVDMTAQLLKAKEAGAEAVLTYGIGPELAQIANG
MAKLGWKVPLIGSWTLSMANYIDNASTNGEGARMPQTFIQEANTPKRKAFIDAYLKEFKPKNNRIDSPVSAAQGYDSIYL
LAAAITQAGSTDGPKVRAALENLNTKVEGVVMVYDKPFTHDDHEAISPNVPVVGEVKGGRVVYAYEADRKGGGQLRTKQA
SAAN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=291, Percent_Identity=22.680412371134, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994941, Length=296, Percent_Identity=21.6216216216216, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43231; Mature: 43100

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIRLKSVVGALALCALANVGYAADPIKIGVDGPFTGGSSSMGVSMRDGVRLATAEINKA
CCEEHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCEECCCCCEEEEEHHHHC
GGVLGRQIVLVERDDEAKNERGVQIAQELINKEKVVAVVGYINTGVALASQRFFQEAKIP
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHCCHHHHHHHHHHHCCCC
VFNNVATGSVVTKQFTDQPDNYVFRNAAADRIQAPMIVEEAVTRRGFRKVAILADSTNYG
EECCCCCCCCHHHHHCCCCCCEEECCCCHHHHCCCHHHHHHHHHCCCEEEEEEECCCCCC
QLGREDLEKALAAKGVKPVAVEKFNIKDVDMTAQLLKAKEAGAEAVLTYGIGPELAQIAN
CCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
GMAKLGWKVPLIGSWTLSMANYIDNASTNGEGARMPQTFIQEANTPKRKAFIDAYLKEFK
HHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCC
PKNNRIDSPVSAAQGYDSIYLLAAAITQAGSTDGPKVRAALENLNTKVEGVVMVYDKPFT
CCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCC
HDDHEAISPNVPVVGEVKGGRVVYAYEADRKGGGQLRTKQASAAN
CCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
GIRLKSVVGALALCALANVGYAADPIKIGVDGPFTGGSSSMGVSMRDGVRLATAEINKA
CEEHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCEECCCCCEEEEEHHHHC
GGVLGRQIVLVERDDEAKNERGVQIAQELINKEKVVAVVGYINTGVALASQRFFQEAKIP
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHCCHHHHHHHHHHHCCCC
VFNNVATGSVVTKQFTDQPDNYVFRNAAADRIQAPMIVEEAVTRRGFRKVAILADSTNYG
EECCCCCCCCHHHHHCCCCCCEEECCCCHHHHCCCHHHHHHHHHCCCEEEEEEECCCCCC
QLGREDLEKALAAKGVKPVAVEKFNIKDVDMTAQLLKAKEAGAEAVLTYGIGPELAQIAN
CCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
GMAKLGWKVPLIGSWTLSMANYIDNASTNGEGARMPQTFIQEANTPKRKAFIDAYLKEFK
HHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCC
PKNNRIDSPVSAAQGYDSIYLLAAAITQAGSTDGPKVRAALENLNTKVEGVVMVYDKPFT
CCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCC
HDDHEAISPNVPVVGEVKGGRVVYAYEADRKGGGQLRTKQASAAN
CCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]