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The map label for this gene is gadA [H]

Identifier: 91782018

GI number: 91782018

Start: 720325

End: 721710

Strand: Direct

Name: gadA [H]

Synonym: Bxe_A3826

Alternate gene names: 91782018

Gene position: 720325-721710 (Clockwise)

Preceding gene: 91782015

Following gene: 91782019

Centisome position: 14.71

GC content: 62.7

Gene sequence:

>1386_bases
ATGACTTTTCTCAAATCACCACCGCCCACGCCCGAGGACGTCGCCGATGAATACGCCGCCACGATCTCGGGTTCATCGCT
GCCCAAGTACCGGATTCCGGAGGCGTCGTCCGATCGCCGCGCGGTATTCGATCTGGTGCGCGACGAACTGTTCATGGACG
GCAATTCACGGCAGAACGTCGCGACGTTCTGCACCACGTATGCCGACGACGAAGTGCGCCACCTGATGGATCTGTCGATC
GACAAGAACATGATCGACAAGGACGAGTATCCGCAAACGGCGGAGATCGAAATGCGCTGCGTGCACATGCTCGCGGATCT
GTGGCATGCCAGCAAGGCATGGAAGACCACCGGATGTTCGACCACGGGTTCGAGCGAGGCTTGCATGCTCGGCGGTCTCG
CGCTCAAGTGGCAATGGAAGAAGCGCCGGGAAGCACAGGGCAAGCCGACCGACAAGCCGAACTTCGTGTGCGGACCGGTG
CAGGTGTGCTGGGCCAAATTCGCCCGCTACTTCGACGTCGAGATGCGCCAGGTGCCCTTGCGCGGCGAGGCAACCGGGCT
GACGCCGGAAGATCTGGCCGAGTATTGCGACGAGAACACGATCGGCGTGGTGGCCACACTCGGCATTACCTTCACCTGCG
TCTATGAACCGGTGAAGGCGCTGGCAGGCGCACTCGACGCGTTGCAAGCCAATGTCGGACTCGATATTCCGATTCACGTC
GACGCTGCCAGCGGCGGCTTCGTCGCGCCGTTCATCCAGCCGGACCTCGAGTGGGACTTCAGCGTGCCGCGCGTGAAATC
CATCAACGCGTCGGGCCACAAATACGGACTCGCGCCGCTCGGGGTCGGCTGGGTAGTCTGGCGCAGCACTCAGGATCTGC
CCGACGAACTGATTTTCCGGGTCGATTATCTGGGCGGCGACATGCCCACGTTCGCACTGAATTTCTCCCGTCCGGCCGGA
CAGATCATCGCGCAGTACTACATGCTGCTGCGCCTGGGCCGCGAAGGCTACCGGCGGATCCAGCAGGAATGCGCCGACAC
CGCGCAGGCGCTGGCCGACGGGCTGGCGAAGATCGACCAGCTGGAAATGATCTACGACGGGCGCGGCGCGCTGCCGGCTG
TCTGCTACAAGCTGAAGCATCCGGAAACAGCGGGCTTCACGCTATTCGACCTGTCCGATCAGGTCCGCATGCGCGGCTGG
CAGATCGCCTCGTACGAACTGCCGGCAGGGCGGGAAGACACCATCGTGCAACGCGTGTTGATCCGGCGTGGTGTGACGCG
CGACATGGCGGCGATGCTGCTTGAAGACATCAGGCACGCCATCGGGCATCTGACGAAGAATCCGGTGCTGCATTCGAGCG
CCGGTCCGACCTTCCACCACAATTGA

Upstream 100 bases:

>100_bases
TTTTTCTGCCTACCTGACATCTTCATTTCATTCGCTCCGGCATTCGTCACGGGATCGTCACGAGCCGGGCACGCTCACTT
CAAATCAGGAGCTTCACGCC

Downstream 100 bases:

>100_bases
TATCGCCTGTTCACAGGGGCACACGAAATGGACAACACAGTCACCGCCGCGGCCGCAGCGCCGGCAGCGGACGCCGCAAA
ACGCGGCAAGTATCTGAGCG

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTFLKSPPPTPEDVADEYAATISGSSLPKYRIPEASSDRRAVFDLVRDELFMDGNSRQNVATFCTTYADDEVRHLMDLSI
DKNMIDKDEYPQTAEIEMRCVHMLADLWHASKAWKTTGCSTTGSSEACMLGGLALKWQWKKRREAQGKPTDKPNFVCGPV
QVCWAKFARYFDVEMRQVPLRGEATGLTPEDLAEYCDENTIGVVATLGITFTCVYEPVKALAGALDALQANVGLDIPIHV
DAASGGFVAPFIQPDLEWDFSVPRVKSINASGHKYGLAPLGVGWVVWRSTQDLPDELIFRVDYLGGDMPTFALNFSRPAG
QIIAQYYMLLRLGREGYRRIQQECADTAQALADGLAKIDQLEMIYDGRGALPAVCYKLKHPETAGFTLFDLSDQVRMRGW
QIASYELPAGREDTIVQRVLIRRGVTRDMAAMLLEDIRHAIGHLTKNPVLHSSAGPTFHHN

Sequences:

>Translated_461_residues
MTFLKSPPPTPEDVADEYAATISGSSLPKYRIPEASSDRRAVFDLVRDELFMDGNSRQNVATFCTTYADDEVRHLMDLSI
DKNMIDKDEYPQTAEIEMRCVHMLADLWHASKAWKTTGCSTTGSSEACMLGGLALKWQWKKRREAQGKPTDKPNFVCGPV
QVCWAKFARYFDVEMRQVPLRGEATGLTPEDLAEYCDENTIGVVATLGITFTCVYEPVKALAGALDALQANVGLDIPIHV
DAASGGFVAPFIQPDLEWDFSVPRVKSINASGHKYGLAPLGVGWVVWRSTQDLPDELIFRVDYLGGDMPTFALNFSRPAG
QIIAQYYMLLRLGREGYRRIQQECADTAQALADGLAKIDQLEMIYDGRGALPAVCYKLKHPETAGFTLFDLSDQVRMRGW
QIASYELPAGREDTIVQRVLIRRGVTRDMAAMLLEDIRHAIGHLTKNPVLHSSAGPTFHHN
>Mature_460_residues
TFLKSPPPTPEDVADEYAATISGSSLPKYRIPEASSDRRAVFDLVRDELFMDGNSRQNVATFCTTYADDEVRHLMDLSID
KNMIDKDEYPQTAEIEMRCVHMLADLWHASKAWKTTGCSTTGSSEACMLGGLALKWQWKKRREAQGKPTDKPNFVCGPVQ
VCWAKFARYFDVEMRQVPLRGEATGLTPEDLAEYCDENTIGVVATLGITFTCVYEPVKALAGALDALQANVGLDIPIHVD
AASGGFVAPFIQPDLEWDFSVPRVKSINASGHKYGLAPLGVGWVVWRSTQDLPDELIFRVDYLGGDMPTFALNFSRPAGQ
IIAQYYMLLRLGREGYRRIQQECADTAQALADGLAKIDQLEMIYDGRGALPAVCYKLKHPETAGFTLFDLSDQVRMRGWQ
IASYELPAGREDTIVQRVLIRRGVTRDMAAMLLEDIRHAIGHLTKNPVLHSSAGPTFHHN

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=430, Percent_Identity=23.4883720930233, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1789934, Length=435, Percent_Identity=55.632183908046, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1787769, Length=447, Percent_Identity=55.0335570469799, Blast_Score=516, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17557272, Length=404, Percent_Identity=25.7425742574257, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25148342, Length=377, Percent_Identity=24.4031830238727, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17543922, Length=333, Percent_Identity=23.1231231231231, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323905, Length=463, Percent_Identity=38.6609071274298, Blast_Score=308, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6320500, Length=357, Percent_Identity=22.4089635854342, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355963, Length=371, Percent_Identity=25.3369272237197, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24654344, Length=371, Percent_Identity=25.3369272237197, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 51285; Mature: 51154

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLKSPPPTPEDVADEYAATISGSSLPKYRIPEASSDRRAVFDLVRDELFMDGNSRQNV
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCH
ATFCTTYADDEVRHLMDLSIDKNMIDKDEYPQTAEIEMRCVHMLADLWHASKAWKTTGCS
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
TTGSSEACMLGGLALKWQWKKRREAQGKPTDKPNFVCGPVQVCWAKFARYFDVEMRQVPL
CCCCCCCEEECCEEEEEEHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHCCHHHCCCC
RGEATGLTPEDLAEYCDENTIGVVATLGITFTCVYEPVKALAGALDALQANVGLDIPIHV
CCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DAASGGFVAPFIQPDLEWDFSVPRVKSINASGHKYGLAPLGVGWVVWRSTQDLPDELIFR
ECCCCCEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECCCCCCCHHHHEE
VDYLGGDMPTFALNFSRPAGQIIAQYYMLLRLGREGYRRIQQECADTAQALADGLAKIDQ
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEMIYDGRGALPAVCYKLKHPETAGFTLFDLSDQVRMRGWQIASYELPAGREDTIVQRVL
HHHEECCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHCCEEEEEEECCCCCHHHHHHHHH
IRRGVTRDMAAMLLEDIRHAIGHLTKNPVLHSSAGPTFHHN
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCC
>Mature Secondary Structure 
TFLKSPPPTPEDVADEYAATISGSSLPKYRIPEASSDRRAVFDLVRDELFMDGNSRQNV
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCH
ATFCTTYADDEVRHLMDLSIDKNMIDKDEYPQTAEIEMRCVHMLADLWHASKAWKTTGCS
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
TTGSSEACMLGGLALKWQWKKRREAQGKPTDKPNFVCGPVQVCWAKFARYFDVEMRQVPL
CCCCCCCEEECCEEEEEEHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHCCHHHCCCC
RGEATGLTPEDLAEYCDENTIGVVATLGITFTCVYEPVKALAGALDALQANVGLDIPIHV
CCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DAASGGFVAPFIQPDLEWDFSVPRVKSINASGHKYGLAPLGVGWVVWRSTQDLPDELIFR
ECCCCCEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECCCCCCCHHHHEE
VDYLGGDMPTFALNFSRPAGQIIAQYYMLLRLGREGYRRIQQECADTAQALADGLAKIDQ
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEMIYDGRGALPAVCYKLKHPETAGFTLFDLSDQVRMRGWQIASYELPAGREDTIVQRVL
HHHEECCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHCCEEEEEEECCCCCHHHHHHHHH
IRRGVTRDMAAMLLEDIRHAIGHLTKNPVLHSSAGPTFHHN
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]