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The map label for this gene is wcaI [H]

Identifier: 91781984

GI number: 91781984

Start: 677389

End: 678741

Strand: Direct

Name: wcaI [H]

Synonym: Bxe_A3860

Alternate gene names: 91781984

Gene position: 677389-678741 (Clockwise)

Preceding gene: 91781983

Following gene: 91781986

Centisome position: 13.84

GC content: 66.74

Gene sequence:

>1353_bases
ATGAAGATACTGATCTACGGCATCAACTATGCGCCTGAACTGACGGGAGTGGGCAAGTACACGGCGGAAATGGCCGTGCT
GCTGGCTGACCGCGGGCATGAAGTGCGCGTGGTGTGCGCGCCGCCGTATTACCCCGAGTGGCGCGTTTCAGAGGGCTACG
CGTCGTGGCGGTATCAGCACGAGACACGCGACGGCGTGGCGGTCTGGCGCGCGCCGCTGTGGGTGCCGTCGCGCCCGAGC
GGCCTGAAGCGCATGCTGCATCTGGCCACTTTCGCGGCAACCTCGCTGCCGCTGCTCGCATGGCAGGCGCTGTGGCGTCC
GCACGCGGTGATGCTGATCGCACCGACCCTGATGTGCGCGCCGGCTTCGCTGATGCTCGCGCGTATCACACGCGCGAGCG
CATGGCTGCATATTCAGGACTACGAGGTCGATGCGGCCTTCGATCTGGGTCTGCTGAAAAGCTCGCGCGCGGCCCGCACG
GCGCGCTGGATCGAAAGCGCGTTGCTGAAGCGCTTCGATGCGGTGTCGTCGATTACGCGGCAGATGAGCGCGCGCGCGAC
GACCAAGGGTGTCGATGCATCGAAGGTGGTCTGCCTGCCGAACTGGGTCGATGTGTCGACTATTTTCCCGCTTGCACGGC
CGAGCGAATTCCGCCGTCTCCTCGGCATTCCCGCGGAGCAGAAAGTGGTGCTGTACTCCGGCAACATGGGCGCGAAGCAG
GGCATTGAAACACTCGCCGACGCGGCGGTCGCGCTCGCCGCGCGTCCTGACCTCACCTTCGTGTTCTGCGGCAGCGGCGC
CGCAAGAGAGAATCTGCTCGAACGCTGCTCCGGTTTGACGAATTGCGTGTTCATGCCGCTGCAACCGGTGGAACGTCTGA
ACGAACTGCTCAATCTCGCCGATATTCACGTGCTCCCGCAACGCGGCGACGCGGCGGATCTCGTCATGCCGTCCAAGCTC
ACCGGCATGTTCGCGAGCGGCCGCGCAACCATCGCGATGGCGCGCCGTGGCACGGCACTCTACGAAGCCGTGGCGCCGCG
CGGCGTGGTCGTGCCGCCGGACAACGTGAAAGCGCTGGCGGCGGCGATCACGGTGCTGGCGGGCGACGCGGAACGGCGCA
CGGCGCTCGGCAAGGCGGCACGCAATTACGCGGAGCGCGCGCTCTCGCCGGAGTCGACGATTCGCACCTTCGAGGAGCGG
CTCGCGGTGCTGTTGCGTGAGTCGGGGCTAGAAGATGCGGAAGATGCGAAACGTGCGAAAGGCGCGCCACCTTCTTTCGG
TCCGCGGCCTTCAGGAGCCGCGGGCCGGCGGCGACGCAAACCGGCCACGGCGGAGGAAGCCGCGCCGGATTGA

Upstream 100 bases:

>100_bases
TGCCAATATCGCTCGCGGCTGTGCGTTGATGTTGAGCGACTGAGTCGCTTCGCACCACCGCGTTGCGCGCACGAAGCCCT
AACCCGTTCTGGCAGTCACG

Downstream 100 bases:

>100_bases
TCCGGCACGCGGCCTGATCATGAAGCTGATATCACCACGGAGAGCGATAGACGCCGCGCTTGCTCGCATAGGGATCGGCG
TACGTGGTGGTCGCCGCGGC

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKILIYGINYAPELTGVGKYTAEMAVLLADRGHEVRVVCAPPYYPEWRVSEGYASWRYQHETRDGVAVWRAPLWVPSRPS
GLKRMLHLATFAATSLPLLAWQALWRPHAVMLIAPTLMCAPASLMLARITRASAWLHIQDYEVDAAFDLGLLKSSRAART
ARWIESALLKRFDAVSSITRQMSARATTKGVDASKVVCLPNWVDVSTIFPLARPSEFRRLLGIPAEQKVVLYSGNMGAKQ
GIETLADAAVALAARPDLTFVFCGSGAARENLLERCSGLTNCVFMPLQPVERLNELLNLADIHVLPQRGDAADLVMPSKL
TGMFASGRATIAMARRGTALYEAVAPRGVVVPPDNVKALAAAITVLAGDAERRTALGKAARNYAERALSPESTIRTFEER
LAVLLRESGLEDAEDAKRAKGAPPSFGPRPSGAAGRRRRKPATAEEAAPD

Sequences:

>Translated_450_residues
MKILIYGINYAPELTGVGKYTAEMAVLLADRGHEVRVVCAPPYYPEWRVSEGYASWRYQHETRDGVAVWRAPLWVPSRPS
GLKRMLHLATFAATSLPLLAWQALWRPHAVMLIAPTLMCAPASLMLARITRASAWLHIQDYEVDAAFDLGLLKSSRAART
ARWIESALLKRFDAVSSITRQMSARATTKGVDASKVVCLPNWVDVSTIFPLARPSEFRRLLGIPAEQKVVLYSGNMGAKQ
GIETLADAAVALAARPDLTFVFCGSGAARENLLERCSGLTNCVFMPLQPVERLNELLNLADIHVLPQRGDAADLVMPSKL
TGMFASGRATIAMARRGTALYEAVAPRGVVVPPDNVKALAAAITVLAGDAERRTALGKAARNYAERALSPESTIRTFEER
LAVLLRESGLEDAEDAKRAKGAPPSFGPRPSGAAGRRRRKPATAEEAAPD
>Mature_450_residues
MKILIYGINYAPELTGVGKYTAEMAVLLADRGHEVRVVCAPPYYPEWRVSEGYASWRYQHETRDGVAVWRAPLWVPSRPS
GLKRMLHLATFAATSLPLLAWQALWRPHAVMLIAPTLMCAPASLMLARITRASAWLHIQDYEVDAAFDLGLLKSSRAART
ARWIESALLKRFDAVSSITRQMSARATTKGVDASKVVCLPNWVDVSTIFPLARPSEFRRLLGIPAEQKVVLYSGNMGAKQ
GIETLADAAVALAARPDLTFVFCGSGAARENLLERCSGLTNCVFMPLQPVERLNELLNLADIHVLPQRGDAADLVMPSKL
TGMFASGRATIAMARRGTALYEAVAPRGVVVPPDNVKALAAAITVLAGDAERRTALGKAARNYAERALSPESTIRTFEER
LAVLLRESGLEDAEDAKRAKGAPPSFGPRPSGAAGRRRRKPATAEEAAPD

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=414, Percent_Identity=43.719806763285, Blast_Score=305, Evalue=3e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 48904; Mature: 48904

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIYGINYAPELTGVGKYTAEMAVLLADRGHEVRVVCAPPYYPEWRVSEGYASWRYQH
CEEEEEECCCCCCCCCCCHHHHHHHHEEECCCCEEEEEECCCCCCCCEECCCCHHCEEEC
ETRDGVAVWRAPLWVPSRPSGLKRMLHLATFAATSLPLLAWQALWRPHAVMLIAPTLMCA
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHC
PASLMLARITRASAWLHIQDYEVDAAFDLGLLKSSRAARTARWIESALLKRFDAVSSITR
HHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
QMSARATTKGVDASKVVCLPNWVDVSTIFPLARPSEFRRLLGIPAEQKVVLYSGNMGAKQ
HHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCEEEEEECCCCHHH
GIETLADAAVALAARPDLTFVFCGSGAARENLLERCSGLTNCVFMPLQPVERLNELLNLA
HHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHH
DIHVLPQRGDAADLVMPSKLTGMFASGRATIAMARRGTALYEAVAPRGVVVPPDNVKALA
HEEEECCCCCCCCEECCHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCEEECCCCHHHHH
AAITVLAGDAERRTALGKAARNYAERALSPESTIRTFEERLAVLLRESGLEDAEDAKRAK
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
GAPPSFGPRPSGAAGRRRRKPATAEEAAPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKILIYGINYAPELTGVGKYTAEMAVLLADRGHEVRVVCAPPYYPEWRVSEGYASWRYQH
CEEEEEECCCCCCCCCCCHHHHHHHHEEECCCCEEEEEECCCCCCCCEECCCCHHCEEEC
ETRDGVAVWRAPLWVPSRPSGLKRMLHLATFAATSLPLLAWQALWRPHAVMLIAPTLMCA
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHC
PASLMLARITRASAWLHIQDYEVDAAFDLGLLKSSRAARTARWIESALLKRFDAVSSITR
HHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
QMSARATTKGVDASKVVCLPNWVDVSTIFPLARPSEFRRLLGIPAEQKVVLYSGNMGAKQ
HHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCEEEEEECCCCHHH
GIETLADAAVALAARPDLTFVFCGSGAARENLLERCSGLTNCVFMPLQPVERLNELLNLA
HHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHH
DIHVLPQRGDAADLVMPSKLTGMFASGRATIAMARRGTALYEAVAPRGVVVPPDNVKALA
HEEEECCCCCCCCEECCHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCEEECCCCHHHHH
AAITVLAGDAERRTALGKAARNYAERALSPESTIRTFEERLAVLLRESGLEDAEDAKRAK
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
GAPPSFGPRPSGAAGRRRRKPATAEEAAPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]