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The map label for this gene is miaA [H]

Identifier: 91781910

GI number: 91781910

Start: 592983

End: 593930

Strand: Reverse

Name: miaA [H]

Synonym: Bxe_A3935

Alternate gene names: 91781910

Gene position: 593930-592983 (Counterclockwise)

Preceding gene: 91781911

Following gene: 91781905

Centisome position: 12.13

GC content: 66.24

Gene sequence:

>948_bases
ATGACTTCACGCACGCCTACCACCGTCCCGTGTCTGCTCGGCCCGACTGCATCCGGCAAAACCGCCGCTGCGCTCGCGCT
GGCCGCCCGGCACCCGGTGGAAATCATCAGTGTCGATTCGGCGCTGGTCTATCGCGAGATGGATATCGGCACCGCCAAAC
CCACCGCCGAAGAGCGTGCGGTGGCGCCGCATCATCTGATCGATATCGTCGATCCCACGGACAGCTATTCCGCCGCACAG
TTTCGCGCCGACACCTTGCGGCTGACCGGCGAGATCCACGCGCGTGGGCGCCTGCCGTTGCTGGTCGGCGGCACCATGCT
GTACTACAAGGCGCTCACCCAGGGGCTCAACGATCTGCCCGCCGCCGACGCCGACCTGCGCGCCACACTCGACGCCGACG
CCGCACGCGAGGGCTGGCCGGCCATGCATGCGCGGCTTGCCGCAGTCGATCCCGTGACGGCCGCGCGCTTGGCGCCGAAC
GATTCGCAGCGGATTCAGCGGGCGCTGGAAGTTTTCATGCTGACCGGACAGGCCATGTCGGCGTTGCTGGCGGCGCCCGC
GCGGGTGGATGATGCCGCCGCCGCGTGGCGCTTCGTGCCAGTTGCGCTGGAGCCGTCCGATCGTGGCGTGCTCCATGCGC
GCATCGAGAAACGCTTCGATGCGATGCTGGCCAACGGTTTCGTCGATGAAGTGGTGAAGTTGCGCGAACGCGGCGACTTA
TCGCCCGAGATGCCGTCCATGCGTTGCGTGGGCTACCGGCAGGTCTGGGAATATCTGGACGGCGCCGTCGACTATTCGAC
CATGCGCGATAAGGGCGTCTTTGCGACCCGGCAGTTGTGCAAGCGGCAGCTCACATGGCTGCGAAGCATGACAGAGCGAG
TGGTGGTGGACTGCTGCGATCCGCACGCGACGGCGCGGGTGCTTGAAGCGATTGAAGCGTTGCTTTGA

Upstream 100 bases:

>100_bases
GTGGTATCAGTTGACGCTGTCCGATCTCGATCGGCTGTTCATGCGTGGTCAGTGACGTGACGCTGCTGCAACCGCCGCTG
CAACGCACGCCCGCCTGCCC

Downstream 100 bases:

>100_bases
AACTTTGACGCTTTGAAACTCTGATGCGTTGAAGCTTCAAACCCGCGCGGCGCCGGCTAACCGCACGCCGCGCGGTCTGA
CAAGCTTTTAGATCACTACG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTSRTPTTVPCLLGPTASGKTAAALALAARHPVEIISVDSALVYREMDIGTAKPTAEERAVAPHHLIDIVDPTDSYSAAQ
FRADTLRLTGEIHARGRLPLLVGGTMLYYKALTQGLNDLPAADADLRATLDADAAREGWPAMHARLAAVDPVTAARLAPN
DSQRIQRALEVFMLTGQAMSALLAAPARVDDAAAAWRFVPVALEPSDRGVLHARIEKRFDAMLANGFVDEVVKLRERGDL
SPEMPSMRCVGYRQVWEYLDGAVDYSTMRDKGVFATRQLCKRQLTWLRSMTERVVVDCCDPHATARVLEAIEALL

Sequences:

>Translated_315_residues
MTSRTPTTVPCLLGPTASGKTAAALALAARHPVEIISVDSALVYREMDIGTAKPTAEERAVAPHHLIDIVDPTDSYSAAQ
FRADTLRLTGEIHARGRLPLLVGGTMLYYKALTQGLNDLPAADADLRATLDADAAREGWPAMHARLAAVDPVTAARLAPN
DSQRIQRALEVFMLTGQAMSALLAAPARVDDAAAAWRFVPVALEPSDRGVLHARIEKRFDAMLANGFVDEVVKLRERGDL
SPEMPSMRCVGYRQVWEYLDGAVDYSTMRDKGVFATRQLCKRQLTWLRSMTERVVVDCCDPHATARVLEAIEALL
>Mature_314_residues
TSRTPTTVPCLLGPTASGKTAAALALAARHPVEIISVDSALVYREMDIGTAKPTAEERAVAPHHLIDIVDPTDSYSAAQF
RADTLRLTGEIHARGRLPLLVGGTMLYYKALTQGLNDLPAADADLRATLDADAAREGWPAMHARLAAVDPVTAARLAPND
SQRIQRALEVFMLTGQAMSALLAAPARVDDAAAAWRFVPVALEPSDRGVLHARIEKRFDAMLANGFVDEVVKLRERGDLS
PEMPSMRCVGYRQVWEYLDGAVDYSTMRDKGVFATRQLCKRQLTWLRSMTERVVVDCCDPHATARVLEAIEALL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=29.903536977492, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1790613, Length=304, Percent_Identity=52.3026315789474, Blast_Score=308, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI25144712, Length=276, Percent_Identity=28.6231884057971, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=28.2312925170068, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24649830, Length=295, Percent_Identity=31.1864406779661, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 34328; Mature: 34197

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRTPTTVPCLLGPTASGKTAAALALAARHPVEIISVDSALVYREMDIGTAKPTAEERA
CCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEEEECHHHEEEECCCCCCCCCHHHHC
VAPHHLIDIVDPTDSYSAAQFRADTLRLTGEIHARGRLPLLVGGTMLYYKALTQGLNDLP
CCCHHEEEEECCCCCCCHHHHHHHHEEEEEEEECCCCCEEEECHHHHHHHHHHHCCCCCC
AADADLRATLDADAAREGWPAMHARLAAVDPVTAARLAPNDSQRIQRALEVFMLTGQAMS
CCCCCHHEECCCCHHHCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ALLAAPARVDDAAAAWRFVPVALEPSDRGVLHARIEKRFDAMLANGFVDEVVKLRERGDL
HHHHCCCCCCHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SPEMPSMRCVGYRQVWEYLDGAVDYSTMRDKGVFATRQLCKRQLTWLRSMTERVVVDCCD
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PHATARVLEAIEALL
CHHHHHHHHHHHHHC
>Mature Secondary Structure 
TSRTPTTVPCLLGPTASGKTAAALALAARHPVEIISVDSALVYREMDIGTAKPTAEERA
CCCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEEEECHHHEEEECCCCCCCCCHHHHC
VAPHHLIDIVDPTDSYSAAQFRADTLRLTGEIHARGRLPLLVGGTMLYYKALTQGLNDLP
CCCHHEEEEECCCCCCCHHHHHHHHEEEEEEEECCCCCEEEECHHHHHHHHHHHCCCCCC
AADADLRATLDADAAREGWPAMHARLAAVDPVTAARLAPNDSQRIQRALEVFMLTGQAMS
CCCCCHHEECCCCHHHCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ALLAAPARVDDAAAAWRFVPVALEPSDRGVLHARIEKRFDAMLANGFVDEVVKLRERGDL
HHHHCCCCCCHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SPEMPSMRCVGYRQVWEYLDGAVDYSTMRDKGVFATRQLCKRQLTWLRSMTERVVVDCCD
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PHATARVLEAIEALL
CHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA