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The map label for this gene is grpE [H]

Identifier: 91781882

GI number: 91781882

Start: 563867

End: 564451

Strand: Direct

Name: grpE [H]

Synonym: Bxe_A3963

Alternate gene names: 91781882

Gene position: 563867-564451 (Clockwise)

Preceding gene: 91781881

Following gene: 91781883

Centisome position: 11.52

GC content: 66.84

Gene sequence:

>585_bases
ATGGAAAACACGCAAGAGAACCCGACGAGCCAGAATCCCAAGCCCGCCGAAGAAACCGCGCGCCAGGCCGCCGAGGCCGC
GGCACCTCAACAGGAAGCAGCGGCGAACGCGGCGACCGACTCGCCGGCGAGCGCCGAGCAGGCTGCACTCGCCGAGGCCC
AGGCGAAGATCGCCGAGCTGCAGGAAAGCTTCCTGCGCGCCAAGGCGGAGACCGAAAACGTGCGCCGCCGCGCTCAGGAA
GACGTCGCCAAGGCCCACAAGTTTGCGATCGAGAGCTTTGCCGAGCATCTGCTGCCGGTGATCGACAGCCTCGAAGCAGC
CGTCGCGCACTCGTCCGACGATCCTGCGAAGGTGCGCGAAGGCGTCGAACTCACGCTGCGCCAGCTTACCGGCGCGCTGG
AAAAGGGGCGCGTCGTCGCCCTGAATCCGGTGGGCGAGAAGTTCGACCCGCATCGCCATCAAGCCATTTCCATGGTGCCG
GCCGACCAGGAGCCGAACACCGTGGTGGCCGTGCTGCAAAAAGGCTTCGTGATCGCGGATCGGGTGCTGCGTCCGGCACT
CGTGACCGTCGCGGCCCCGAAGTAA

Upstream 100 bases:

>100_bases
GTCTGTCGCGATTTTGCAACGCACAAACCGCGCCAGGTTTTTGCGCTGCCGTTACTGCCGGCTTTTATCTCGCTTTCACT
TTTTAACGACTTTCAGCGAC

Downstream 100 bases:

>100_bases
GCGCCGCCGGTCTTTCGTAAGCCGTTTCGTCCGGCAGGCGCCGTCATGGCCAACATTCCCGTCGACTCCACCGCGTTCGC
GGTGTTCGGCATGCAGGAGT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MENTQENPTSQNPKPAEETARQAAEAAAPQQEAAANAATDSPASAEQAALAEAQAKIAELQESFLRAKAETENVRRRAQE
DVAKAHKFAIESFAEHLLPVIDSLEAAVAHSSDDPAKVREGVELTLRQLTGALEKGRVVALNPVGEKFDPHRHQAISMVP
ADQEPNTVVAVLQKGFVIADRVLRPALVTVAAPK

Sequences:

>Translated_194_residues
MENTQENPTSQNPKPAEETARQAAEAAAPQQEAAANAATDSPASAEQAALAEAQAKIAELQESFLRAKAETENVRRRAQE
DVAKAHKFAIESFAEHLLPVIDSLEAAVAHSSDDPAKVREGVELTLRQLTGALEKGRVVALNPVGEKFDPHRHQAISMVP
ADQEPNTVVAVLQKGFVIADRVLRPALVTVAAPK
>Mature_194_residues
MENTQENPTSQNPKPAEETARQAAEAAAPQQEAAANAATDSPASAEQAALAEAQAKIAELQESFLRAKAETENVRRRAQE
DVAKAHKFAIESFAEHLLPVIDSLEAAVAHSSDDPAKVREGVELTLRQLTGALEKGRVVALNPVGEKFDPHRHQAISMVP
ADQEPNTVVAVLQKGFVIADRVLRPALVTVAAPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=196, Percent_Identity=34.6938775510204, Blast_Score=94, Evalue=7e-20,
Organism=Homo sapiens, GI40255109, Length=165, Percent_Identity=34.5454545454545, Blast_Score=81, Evalue=5e-16,
Organism=Escherichia coli, GI1788967, Length=194, Percent_Identity=39.1752577319588, Blast_Score=119, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17552458, Length=173, Percent_Identity=33.5260115606936, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324806, Length=141, Percent_Identity=36.8794326241135, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24653432, Length=172, Percent_Identity=33.7209302325581, Blast_Score=90, Evalue=8e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 20718; Mature: 20718

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTQENPTSQNPKPAEETARQAAEAAAPQQEAAANAATDSPASAEQAALAEAQAKIAEL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QESFLRAKAETENVRRRAQEDVAKAHKFAIESFAEHLLPVIDSLEAAVAHSSDDPAKVRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GVELTLRQLTGALEKGRVVALNPVGEKFDPHRHQAISMVPADQEPNTVVAVLQKGFVIAD
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCHHHHH
RVLRPALVTVAAPK
HHHHHHHHHCCCCC
>Mature Secondary Structure
MENTQENPTSQNPKPAEETARQAAEAAAPQQEAAANAATDSPASAEQAALAEAQAKIAEL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QESFLRAKAETENVRRRAQEDVAKAHKFAIESFAEHLLPVIDSLEAAVAHSSDDPAKVRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GVELTLRQLTGALEKGRVVALNPVGEKFDPHRHQAISMVPADQEPNTVVAVLQKGFVIAD
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCHHHHH
RVLRPALVTVAAPK
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA