Click here to switch to the map view.

The map label for this gene is citH [H]

Identifier: 91781851

GI number: 91781851

Start: 522470

End: 523747

Strand: Direct

Name: citH [H]

Synonym: Bxe_A3994

Alternate gene names: 91781851

Gene position: 522470-523747 (Clockwise)

Preceding gene: 91781850

Following gene: 91781857

Centisome position: 10.67

GC content: 63.77

Gene sequence:

>1278_bases
ATGAACGCAACGACAGCAGCAAGCCTCACGGGAAGCCGGCAGAGTTCGTGGCGCGCGGTGGTCGCGGCCTCGATCGGCAA
CGCGTTGGAATGGTTCGATCTGGTGGTGTACGGTTTTTTCGCGGTGGTGATCGCGAAGCTGTTTTTCCCGGCCGGTAACG
ACACGGTCTCGCTGCTTCTCACGCTCGGCACGTTCGGCGTGTCGTTCTTCATGCGGCCGCTCGGCGCGATCGTGATCGGC
GCCTATGCCGACCGTGCCGGCCGCAAGGCGGCGCTCACCCTGTCGATCCTGCTGATGATGGCCGGCACGCTGATCATCGC
GATTCTGCCGACCTATCAGAGCATCGGTCTCGCCGCGCCGGTGATCCTCGTGTTGGCGCGTCTGATGCAGGGCTTTTCGG
CGGGCGGCGAGTTCGGCAGCGCTACCGCCTTTCTCGCGGAACACGTACCGGGGCGGCGCGGTTTTTTCGCGAGCTGGCAG
GTGGCGAGCCAGGGGCTCACCACGCTGCTGGCCGCCGGCTTCGGCGTGCTGCTCACCGGCCAGCTCTCGCCGGAGCAGAT
GAGTTCCTGGGGTTGGCGCGTGCCGTTCTTCTTTGGTCTGCTGATCGGGCCGGTCGCCTGGTATATCCGCACGAAGCTCG
ACGAGACGCCTGAATTCCTCGCGGCCGAAACGACCACGACGCCGCTGCGCGATACCTTCACCAGCCAGAAATTGCGCCTG
CTGATCGCCATCGGCGTGGTGGTGCTCGGGACGGTGTCGACCTATCTGGTGCTGTTCATGCCGACTTTCGGCGTGAAGCA
GTTGGGACTGGCGCCCTCGGTGGCGTTCGCGGCGATCGCATTGACCGGCTTGATCCAGATGGTGTTCTCGCCGCTGATCG
GCCATCTGTCGGACCGGCATGGGCGCACCACGATCATGCTGGCTTCGGCGTTGCTGCTGCTTGTCCTGATCTACCCGGCT
TTCGTGTATCTGGTTGCGCATCCGACCTTCGGCACCCTGATCGTCATGCAGATCGTGTTCGGCTTTCTGATGACCGGGTA
TTTCGCGGCGCTGCCGGGTCTGCTTTCGGAGATGTTTCCGGTGCAAACGCGTACGACCGGCATGTCGCTCGCCTATAACA
TTGCCGTGACGATTTTCGGCGGTTTCGGGCCGTTCATCATTGCGTGGCTGATCAGCGTGACGGGCTCGAAGGCCGCGCCG
AGTTATTACCTGATTTTCGCCGCGGCCGTGAGCCTCGTTGCGCTGATCGCGGCGCGGCGCAAGCTCGGCTTTCGCTGA

Upstream 100 bases:

>100_bases
ACATAGAGCGCAGGCACCGCATTGGTTGTGCGCGCATCCAGTCGGGCATTCAGGCGTGAAGCCGACATCAATAATATTTT
CGCGCGAATCGGAGATCTGC

Downstream 100 bases:

>100_bases
GGCCGGCGCCGTGCGTTGCGGCTGCGAGATGGCGCAGCGCGACGGCTGAGCAGGGCCGGAGGGCGGCGGTAAACGCAAAA
GGCCGGTTCAATCATGAACC

Product: major facilitator superfamily metabolite/H(+) symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNATTAASLTGSRQSSWRAVVAASIGNALEWFDLVVYGFFAVVIAKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVIG
AYADRAGRKAALTLSILLMMAGTLIIAILPTYQSIGLAAPVILVLARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGVLLTGQLSPEQMSSWGWRVPFFFGLLIGPVAWYIRTKLDETPEFLAAETTTTPLRDTFTSQKLRL
LIAIGVVVLGTVSTYLVLFMPTFGVKQLGLAPSVAFAAIALTGLIQMVFSPLIGHLSDRHGRTTIMLASALLLLVLIYPA
FVYLVAHPTFGTLIVMQIVFGFLMTGYFAALPGLLSEMFPVQTRTTGMSLAYNIAVTIFGGFGPFIIAWLISVTGSKAAP
SYYLIFAAAVSLVALIAARRKLGFR

Sequences:

>Translated_425_residues
MNATTAASLTGSRQSSWRAVVAASIGNALEWFDLVVYGFFAVVIAKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVIG
AYADRAGRKAALTLSILLMMAGTLIIAILPTYQSIGLAAPVILVLARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGVLLTGQLSPEQMSSWGWRVPFFFGLLIGPVAWYIRTKLDETPEFLAAETTTTPLRDTFTSQKLRL
LIAIGVVVLGTVSTYLVLFMPTFGVKQLGLAPSVAFAAIALTGLIQMVFSPLIGHLSDRHGRTTIMLASALLLLVLIYPA
FVYLVAHPTFGTLIVMQIVFGFLMTGYFAALPGLLSEMFPVQTRTTGMSLAYNIAVTIFGGFGPFIIAWLISVTGSKAAP
SYYLIFAAAVSLVALIAARRKLGFR
>Mature_425_residues
MNATTAASLTGSRQSSWRAVVAASIGNALEWFDLVVYGFFAVVIAKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVIG
AYADRAGRKAALTLSILLMMAGTLIIAILPTYQSIGLAAPVILVLARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGVLLTGQLSPEQMSSWGWRVPFFFGLLIGPVAWYIRTKLDETPEFLAAETTTTPLRDTFTSQKLRL
LIAIGVVVLGTVSTYLVLFMPTFGVKQLGLAPSVAFAAIALTGLIQMVFSPLIGHLSDRHGRTTIMLASALLLLVLIYPA
FVYLVAHPTFGTLIVMQIVFGFLMTGYFAALPGLLSEMFPVQTRTTGMSLAYNIAVTIFGGFGPFIIAWLISVTGSKAAP
SYYLIFAAAVSLVALIAARRKLGFR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=412, Percent_Identity=33.7378640776699, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1788942, Length=435, Percent_Identity=35.8620689655172, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788292, Length=405, Percent_Identity=30.1234567901235, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=33.2541567695962, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87082231, Length=220, Percent_Identity=29.0909090909091, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87082404, Length=389, Percent_Identity=23.3933161953728, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45313; Mature: 45313

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATTAASLTGSRQSSWRAVVAASIGNALEWFDLVVYGFFAVVIAKLFFPAGNDTVSLLL
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
TLGTFGVSFFMRPLGAIVIGAYADRAGRKAALTLSILLMMAGTLIIAILPTYQSIGLAAP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VILVLARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQVASQGLTTLLAAGFGVLLTG
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEC
QLSPEQMSSWGWRVPFFFGLLIGPVAWYIRTKLDETPEFLAAETTTTPLRDTFTSQKLRL
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHH
LIAIGVVVLGTVSTYLVLFMPTFGVKQLGLAPSVAFAAIALTGLIQMVFSPLIGHLSDRH
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GRTTIMLASALLLLVLIYPAFVYLVAHPTFGTLIVMQIVFGFLMTGYFAALPGLLSEMFP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VQTRTTGMSLAYNIAVTIFGGFGPFIIAWLISVTGSKAAPSYYLIFAAAVSLVALIAARR
CCCCCCCHHHHEEEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
KLGFR
HHCCC
>Mature Secondary Structure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HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]