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The map label for this gene is rlmL [H]

Identifier: 91781842

GI number: 91781842

Start: 512576

End: 513868

Strand: Reverse

Name: rlmL [H]

Synonym: Bxe_A4003

Alternate gene names: 91781842

Gene position: 513868-512576 (Counterclockwise)

Preceding gene: 91781843

Following gene: 91781841

Centisome position: 10.5

GC content: 66.43

Gene sequence:

>1293_bases
ATGTCCTTCGATTTCTTCCTCCCTTGCCCACGCGGCCTTGAAGCCTCGCTCGCGGCCGAACTCGCCGAAATTGCCGCCCA
GCACCTGAACGGCGCGCCGTTTACAGCCGGCGCACAAGTGCCGGGCGGCGTGCATTTCCGCGGAGGCTGGGCCGCCGGCA
TGGCCGCCAACCTGCACTCGCGCCTCGCGAGCCGCGTGCTGCTGAAAGTCGCGCATCGTCCGTATCGCAGCGAGCAGGAC
ATTTACGCGCTCGCGGTCGAACAGCGTTGGGAACAATGGTTCTCGGCAAACGAAACGCTGCGGGTGGACGTCACCGCGAT
CAAATCGCCGCTGCGCAGCCTGGAATTCACCACACTGCGCGTGAAGGACGCGATCTGCGACCGTCTGCGCGAAGTCAGCG
GCGCGCGTCCGAGTATCGACACCGCCATGCCCGACGTGCGCGTGTTCGCGTTTCTCACCGCCACCGACTGCACGCTGTAT
CTCGACACCTCGGGCGACCCGCTCTTCAAGCGCGGCTGGCGCCTCGACAAGGGTGCGGCGCCGCTGCGCGAGAATCTGGC
CGCGGGCATTCTGCGTCTGACCGGCTGGACCGCCGGCACGCCCCTGTACGACCCCATGTGCGGCAGCGGCACGTTCCTCG
CGGAAGCCGCGCAGGTGGCGCTGAATATCGCGCCGGGCGCGGAACGCCGCTTCGGCTTCGAGAAGCTCAAGCAATACGAG
GCCAAAACCTGGCAAACGCTCAAGGCCGCCGCGCTCGACGCGAAACACGCCGCGCGTAATTCACGCGCCGATCTGCAGAT
CTTCGGCAGCGATATTTCCGGCGACATGCTCGACAAGGCGCGCGCCAACTTCCAGCGCGCCGGCTTGCCGTCCATTCCGC
TCAAGCAGCTCGACGCGCGCAACATGACGCCGCCCTCATCCGCGGCGGGCATTCTCGTCGCGAATCCGCCCTATGGCGAA
CGGATCGAAGTGCGCGGACGCAATGCGCGCGGCGAGCTTCGTGAAGGCCGCGGCAATCGTGACAGCCGCGAAGACGACGG
CTTTCGCCGCGCCCAGGAGGAAACGCCGGACAGCGAATTCTTCCAGTCGCTCGGCGACGCGCTCAAGCAACGCTTCACCG
GTTGGCATGCGTTCATCCTGACATCGGACCGCAAGCTGCCCGGCCAGTTGCGTCTGCGTGAATCGACCAAGACACCGCTC
TTTAACGGCGCGCTCGAATGCCGGCTGTTCCGCTTCGATCTGATCGCGGGCAGCGTGCGGCAGCGGCCGCAAACCGATAC
GCCGGCGGCTTGA

Upstream 100 bases:

>100_bases
CGCGCCCAGCCGGCCGCGGCTTGCCGCCAGGCAATCGCGCAGGGCCGCCCACCCCGACCGCGTACAATAGCGCCTTATCC
AACGCCTTCCGGCAACCTCA

Downstream 100 bases:

>100_bases
CGCACAGCGCAGCGCTTTCGGCGCTGCCGCTGCGAGGCGATAAGAACCGCGAGCCGCGAACCGGCCTCGCGGTTTTTTTC
GCCTGCCGCCTGCTTCGGCC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSFDFFLPCPRGLEASLAAELAEIAAQHLNGAPFTAGAQVPGGVHFRGGWAAGMAANLHSRLASRVLLKVAHRPYRSEQD
IYALAVEQRWEQWFSANETLRVDVTAIKSPLRSLEFTTLRVKDAICDRLREVSGARPSIDTAMPDVRVFAFLTATDCTLY
LDTSGDPLFKRGWRLDKGAAPLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQVALNIAPGAERRFGFEKLKQYE
AKTWQTLKAAALDAKHAARNSRADLQIFGSDISGDMLDKARANFQRAGLPSIPLKQLDARNMTPPSSAAGILVANPPYGE
RIEVRGRNARGELREGRGNRDSREDDGFRRAQEETPDSEFFQSLGDALKQRFTGWHAFILTSDRKLPGQLRLRESTKTPL
FNGALECRLFRFDLIAGSVRQRPQTDTPAA

Sequences:

>Translated_430_residues
MSFDFFLPCPRGLEASLAAELAEIAAQHLNGAPFTAGAQVPGGVHFRGGWAAGMAANLHSRLASRVLLKVAHRPYRSEQD
IYALAVEQRWEQWFSANETLRVDVTAIKSPLRSLEFTTLRVKDAICDRLREVSGARPSIDTAMPDVRVFAFLTATDCTLY
LDTSGDPLFKRGWRLDKGAAPLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQVALNIAPGAERRFGFEKLKQYE
AKTWQTLKAAALDAKHAARNSRADLQIFGSDISGDMLDKARANFQRAGLPSIPLKQLDARNMTPPSSAAGILVANPPYGE
RIEVRGRNARGELREGRGNRDSREDDGFRRAQEETPDSEFFQSLGDALKQRFTGWHAFILTSDRKLPGQLRLRESTKTPL
FNGALECRLFRFDLIAGSVRQRPQTDTPAA
>Mature_429_residues
SFDFFLPCPRGLEASLAAELAEIAAQHLNGAPFTAGAQVPGGVHFRGGWAAGMAANLHSRLASRVLLKVAHRPYRSEQDI
YALAVEQRWEQWFSANETLRVDVTAIKSPLRSLEFTTLRVKDAICDRLREVSGARPSIDTAMPDVRVFAFLTATDCTLYL
DTSGDPLFKRGWRLDKGAAPLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQVALNIAPGAERRFGFEKLKQYEA
KTWQTLKAAALDAKHAARNSRADLQIFGSDISGDMLDKARANFQRAGLPSIPLKQLDARNMTPPSSAAGILVANPPYGER
IEVRGRNARGELREGRGNRDSREDDGFRRAQEETPDSEFFQSLGDALKQRFTGWHAFILTSDRKLPGQLRLRESTKTPLF
NGALECRLFRFDLIAGSVRQRPQTDTPAA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=374, Percent_Identity=32.8877005347594, Blast_Score=191, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 47388; Mature: 47257

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDFFLPCPRGLEASLAAELAEIAAQHLNGAPFTAGAQVPGGVHFRGGWAAGMAANLHS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHH
RLASRVLLKVAHRPYRSEQDIYALAVEQRWEQWFSANETLRVDVTAIKSPLRSLEFTTLR
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCHHEEH
VKDAICDRLREVSGARPSIDTAMPDVRVFAFLTATDCTLYLDTSGDPLFKRGWRLDKGAA
HHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCCCCC
PLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQVALNIAPGAERRFGFEKLKQYE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCHHHHCHHHHHHHH
AKTWQTLKAAALDAKHAARNSRADLQIFGSDISGDMLDKARANFQRAGLPSIPLKQLDAR
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCC
NMTPPSSAAGILVANPPYGERIEVRGRNARGELREGRGNRDSREDDGFRRAQEETPDSEF
CCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHCCCCCCCCCCCHHHHHHHHCCCHHHH
FQSLGDALKQRFTGWHAFILTSDRKLPGQLRLRESTKTPLFNGALECRLFRFDLIAGSVR
HHHHHHHHHHHHCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCEEEEEEHHHHHHHHH
QRPQTDTPAA
CCCCCCCCCC
>Mature Secondary Structure 
SFDFFLPCPRGLEASLAAELAEIAAQHLNGAPFTAGAQVPGGVHFRGGWAAGMAANLHS
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHH
RLASRVLLKVAHRPYRSEQDIYALAVEQRWEQWFSANETLRVDVTAIKSPLRSLEFTTLR
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCHHEEH
VKDAICDRLREVSGARPSIDTAMPDVRVFAFLTATDCTLYLDTSGDPLFKRGWRLDKGAA
HHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCCCCC
PLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQVALNIAPGAERRFGFEKLKQYE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCHHHHCHHHHHHHH
AKTWQTLKAAALDAKHAARNSRADLQIFGSDISGDMLDKARANFQRAGLPSIPLKQLDAR
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCC
NMTPPSSAAGILVANPPYGERIEVRGRNARGELREGRGNRDSREDDGFRRAQEETPDSEF
CCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHCCCCCCCCCCCHHHHHHHHCCCHHHH
FQSLGDALKQRFTGWHAFILTSDRKLPGQLRLRESTKTPLFNGALECRLFRFDLIAGSVR
HHHHHHHHHHHHCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCEEEEEEHHHHHHHHH
QRPQTDTPAA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA