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The map label for this gene is gloA [H]

Identifier: 91781827

GI number: 91781827

Start: 497771

End: 498157

Strand: Direct

Name: gloA [H]

Synonym: Bxe_A4018

Alternate gene names: 91781827

Gene position: 497771-498157 (Clockwise)

Preceding gene: 91781826

Following gene: 91781828

Centisome position: 10.17

GC content: 60.21

Gene sequence:

>387_bases
ATGCGCCTGCTTCACACCATGCTCCGGGTCGGCGACCTGGATCGCTCGATTGCCTTTTACACCGAACTGCTCGGCATGAA
ACTGCTGCGCCGCGACGATTATCCGGACGGCAAATTCACGCTGGCATTCGTCGGTTACGAAGATGAGCGCGACGGCACCG
TGCTCGAACTCACCCACAACTGGGACACGCCTTCTTACGACCTCGGCACCGGTTTCGGCCACCTCGCCGTCGAAGTGGAC
GACGCCTACGCCGCATGTGACAGAATCAAGGCACAAGGCGGCACGGTGGTGCGCGAAGCCGGCCCGATGAAGCACGGCAC
GACCGTGATCGCATTCGTCACGGATCCGGACGGCTACAAGATCGAGTTCATCCAGAAGAAGAAATGA

Upstream 100 bases:

>100_bases
GCATCAGTTCTGAGTGCGGCTTTCCGTTTGGCACGCTCTGCAGCGGCGCGTGATTCCGGGTACATTTACGGTCTCGCTAT
CATGCAACAGGGAGTGCACA

Downstream 100 bases:

>100_bases
AGCAGGGCGGCGGGGCGAGATCGAACGATGAATAACAGCAATCCCTACTTCAAGGAACTCGACGAGATCCACGTCGAACT
CGCGGCCCTGTTCGACGGTT

Product: glyoxalase I

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MRLLHTMLRVGDLDRSIAFYTELLGMKLLRRDDYPDGKFTLAFVGYEDERDGTVLELTHNWDTPSYDLGTGFGHLAVEVD
DAYAACDRIKAQGGTVVREAGPMKHGTTVIAFVTDPDGYKIEFIQKKK

Sequences:

>Translated_128_residues
MRLLHTMLRVGDLDRSIAFYTELLGMKLLRRDDYPDGKFTLAFVGYEDERDGTVLELTHNWDTPSYDLGTGFGHLAVEVD
DAYAACDRIKAQGGTVVREAGPMKHGTTVIAFVTDPDGYKIEFIQKKK
>Mature_128_residues
MRLLHTMLRVGDLDRSIAFYTELLGMKLLRRDDYPDGKFTLAFVGYEDERDGTVLELTHNWDTPSYDLGTGFGHLAVEVD
DAYAACDRIKAQGGTVVREAGPMKHGTTVIAFVTDPDGYKIEFIQKKK

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=152, Percent_Identity=40.1315789473684, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1787940, Length=127, Percent_Identity=63.7795275590551, Blast_Score=174, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=38.8489208633094, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129743, Length=147, Percent_Identity=38.7755102040816, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920404, Length=136, Percent_Identity=28.6764705882353, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14389; Mature: 14389

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGDLDRSIAFYTELLGMKLLRRDDYPDGKFTLAFVGYEDERDGTVLELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
WDTPSYDLGTGFGHLAVEVDDAYAACDRIKAQGGTVVREAGPMKHGTTVIAFVTDPDGYK
CCCCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCE
IEFIQKKK
EEEEEECC
>Mature Secondary Structure
MRLLHTMLRVGDLDRSIAFYTELLGMKLLRRDDYPDGKFTLAFVGYEDERDGTVLELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
WDTPSYDLGTGFGHLAVEVDDAYAACDRIKAQGGTVVREAGPMKHGTTVIAFVTDPDGYK
CCCCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCE
IEFIQKKK
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]