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The map label for this gene is gcvA [H]

Identifier: 91781815

GI number: 91781815

Start: 483023

End: 483955

Strand: Direct

Name: gcvA [H]

Synonym: Bxe_A4030

Alternate gene names: 91781815

Gene position: 483023-483955 (Clockwise)

Preceding gene: 91781813

Following gene: 91781816

Centisome position: 9.87

GC content: 66.67

Gene sequence:

>933_bases
ATGAAACCGATCCCGCCTTTGACCGCGCTGCGCTGCTTCGAGGCCGTCGCCCGCCTGGGCGGCGTGACGCAGGCGGCGCG
CGAACTGCATGTCACGCACTCGGCGGTAAGCCAGCAGATCAAGGTGCTGGAAGAGTCGATGGGCGTCGCACTGTTCATGC
GCGCGGCGCGCGGGCTGCGGCTGACCGAGGAGGGCAGGCTTTACGCGCTCGACATCCGCATGGCGCTGCGCGATATCACG
CATGCCACGCGCCGCGCGCAGGCCCGCCCGCACGAAAGCGAACTGGTGATCACGACCTTGCCGTCGTTTGCTCAGCACTG
GCTGGTGCCGCGTCTCGCCAGTTTTCGCGACGCGCATCCGTATTACCGGATCCGCTTGCTGACGAGTTTGCAGGTCGAGG
ACTTCCGCCAAGGCGTGAGCGATATCGGCATTCGCATGGGTCAGGGGCATTGGCCCGACGTCGCGCAACAGAAACTGTTC
GACGACGAGATGGTGGTGGTCGCGGCGCCGCACTTCGCGCTCGCGCCGGATGGCCGCTTTCCGGTCACTGCCGCGGACGT
GATGGCCTGCCCGCTGATCTCGAGTCCCGATACGCCGTGGCATGAGTGGTGCCAGGCGGCACAGGTGTCGGAGCCGGCCG
AAGGTGCCGTGGTGCTGTCGGCGAACGATTCCAATCTCGTGATCGCCGCCGTGCTGCAGGGCCAGGGCGTCGCGCTCGAA
CGGCGCAGTCTCGTCGCGTATGCGCTCGCGCGGGGCGAACTGCTGCAGATCACCGATATTCGCGTGCCGTACCCTTATCC
GTACTGGCTGGTGTGGCAGCAACGCGAAATCCTCGATGCCGCGCAGGCGCATTTCGCGCAGTGGATAGAAGGTGAGGTCG
ACGCCTACCTGCGCAACGGCAGCGCGCCTGCGGCGGCGATTCGTGCGGGTTAG

Upstream 100 bases:

>100_bases
CATGCCGGCACGGAGTGGGGCGCTTCGAACCGATAGATTAAGCATCTCCGCCGATCAAAAAAAGCGACAATTAACGACGA
TTTACGTCAGAATTTCTTAC

Downstream 100 bases:

>100_bases
CCCGTGCCGGTTCGCGTAAACGGTTTTTCGAAAAACGCTTCGGTCGGCTATATTCGTGGTTCCCATCTCAGACTGCGCTG
GGAACCACGAAGCACCGATT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKPIPPLTALRCFEAVARLGGVTQAARELHVTHSAVSQQIKVLEESMGVALFMRAARGLRLTEEGRLYALDIRMALRDIT
HATRRAQARPHESELVITTLPSFAQHWLVPRLASFRDAHPYYRIRLLTSLQVEDFRQGVSDIGIRMGQGHWPDVAQQKLF
DDEMVVVAAPHFALAPDGRFPVTAADVMACPLISSPDTPWHEWCQAAQVSEPAEGAVVLSANDSNLVIAAVLQGQGVALE
RRSLVAYALARGELLQITDIRVPYPYPYWLVWQQREILDAAQAHFAQWIEGEVDAYLRNGSAPAAAIRAG

Sequences:

>Translated_310_residues
MKPIPPLTALRCFEAVARLGGVTQAARELHVTHSAVSQQIKVLEESMGVALFMRAARGLRLTEEGRLYALDIRMALRDIT
HATRRAQARPHESELVITTLPSFAQHWLVPRLASFRDAHPYYRIRLLTSLQVEDFRQGVSDIGIRMGQGHWPDVAQQKLF
DDEMVVVAAPHFALAPDGRFPVTAADVMACPLISSPDTPWHEWCQAAQVSEPAEGAVVLSANDSNLVIAAVLQGQGVALE
RRSLVAYALARGELLQITDIRVPYPYPYWLVWQQREILDAAQAHFAQWIEGEVDAYLRNGSAPAAAIRAG
>Mature_310_residues
MKPIPPLTALRCFEAVARLGGVTQAARELHVTHSAVSQQIKVLEESMGVALFMRAARGLRLTEEGRLYALDIRMALRDIT
HATRRAQARPHESELVITTLPSFAQHWLVPRLASFRDAHPYYRIRLLTSLQVEDFRQGVSDIGIRMGQGHWPDVAQQKLF
DDEMVVVAAPHFALAPDGRFPVTAADVMACPLISSPDTPWHEWCQAAQVSEPAEGAVVLSANDSNLVIAAVLQGQGVALE
RRSLVAYALARGELLQITDIRVPYPYPYWLVWQQREILDAAQAHFAQWIEGEVDAYLRNGSAPAAAIRAG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=35.2739726027397, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=28.2312925170068, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788706, Length=257, Percent_Identity=28.4046692607004, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=25.3289473684211, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87081978, Length=263, Percent_Identity=28.5171102661597, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=168, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=28.0405405405405, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788887, Length=193, Percent_Identity=32.1243523316062, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788481, Length=282, Percent_Identity=26.241134751773, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790262, Length=244, Percent_Identity=27.8688524590164, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=25.7731958762887, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789639, Length=168, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=243, Percent_Identity=27.9835390946502, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34324; Mature: 34324

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIPPLTALRCFEAVARLGGVTQAARELHVTHSAVSQQIKVLEESMGVALFMRAARGLR
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LTEEGRLYALDIRMALRDITHATRRAQARPHESELVITTLPSFAQHWLVPRLASFRDAHP
EECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCC
YYRIRLLTSLQVEDFRQGVSDIGIRMGQGHWPDVAQQKLFDDEMVVVAAPHFALAPDGRF
EEEEEEEECCCHHHHHCCHHHHCEECCCCCCCCHHHHHHCCCCEEEEECCCEEECCCCCC
PVTAADVMACPLISSPDTPWHEWCQAAQVSEPAEGAVVLSANDSNLVIAAVLQGQGVALE
CCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEH
RRSLVAYALARGELLQITDIRVPYPYPYWLVWQQREILDAAQAHFAQWIEGEVDAYLRNG
HHHHHHHHHHCCCEEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHHCCC
SAPAAAIRAG
CCCCHHCCCC
>Mature Secondary Structure
MKPIPPLTALRCFEAVARLGGVTQAARELHVTHSAVSQQIKVLEESMGVALFMRAARGLR
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LTEEGRLYALDIRMALRDITHATRRAQARPHESELVITTLPSFAQHWLVPRLASFRDAHP
EECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCC
YYRIRLLTSLQVEDFRQGVSDIGIRMGQGHWPDVAQQKLFDDEMVVVAAPHFALAPDGRF
EEEEEEEECCCHHHHHCCHHHHCEECCCCCCCCHHHHHHCCCCEEEEECCCEEECCCCCC
PVTAADVMACPLISSPDTPWHEWCQAAQVSEPAEGAVVLSANDSNLVIAAVLQGQGVALE
CCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEH
RRSLVAYALARGELLQITDIRVPYPYPYWLVWQQREILDAAQAHFAQWIEGEVDAYLRNG
HHHHHHHHHHCCCEEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHHCCC
SAPAAAIRAG
CCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]