Click here to switch to the map view.

The map label for this gene is bah [H]

Identifier: 91781781

GI number: 91781781

Start: 440488

End: 441444

Strand: Direct

Name: bah [H]

Synonym: Bxe_A4064

Alternate gene names: 91781781

Gene position: 440488-441444 (Clockwise)

Preceding gene: 91781780

Following gene: 91781783

Centisome position: 9.0

GC content: 68.23

Gene sequence:

>957_bases
ATGAGCTGGCAAAGCGCACTCGCGTGCTGGTATCTGCGTAGGCAGTTTCGACCCGAGACCCTCAAGCCGGGCATCAATGT
AGAGAGGGCGCGCGCGCTGACCGCCAAACGCGTCTGGTCGCCACGCGTGCCTCGCGGCTGGCATCTGCGTGAGCTGTACG
GCCAGGACGACGCGCCGTTGCGCGGCGAATGGATCGAGCGTGCGGACGGTTCGCCAGTTGCTGGCGCCGGCCCGATCGTG
CTGTACTGCCACGGCGGCGGCTACTATTTCTGTTCACCTCGCACGCATCGCTCGCTCGTGTTCGGTCTGGCGACGCGCGC
CAATGCGGCAATCTTTTCGCTCGACTACCGCCTCGCGCCCGAGCACCGCTTTCCGGCTGCGCTGAACGATACGACGGCGG
CCTATCGCCGGTTGATCGGAGGAGGGATCGCGCCGGAGTCGATCGTGATCGCAGGCGATTCGGCTGGCGGCGGCCTCGCG
CTTGCCACGCTCGTAGCGCTGCGCGACGCCGGCGACCCCATGCCGGCCGGCGGCCTGCTGTTCTCGCCGTGGACCGATCT
GGCCGCGACCGGCGCCAGCATCCACGGCAACGACGGTCTCGATCCGATGTTCAGCGGTCCCGCGATCGCGCCGGCGGGGA
AAATCTATCTAGGCGACACGCCGGCCACGCATCCCTACGCCTCGCCCGTTTATGCCGATCTGCACGGCTTGCCGCCGCTC
TTCATCATGGCCGGCAGCACGGAGGTGTTGCTCGACGACTCGCAGCGCGTGGCCGACAACGCGCGCGCGGCAGGCGTCGA
TTGCGAACTGGAAGTGTGGCAGAAGATGCCGCACGTCTGGCCGATCTTCGCGCCGTTCATCCCGGAAGGAAACCGCGCGC
TCGACCGTGCCGCCGGTTTCGTGCGGCGCGCGACGAGCCGCGCGGCTCAGCCGTCGAGCGCGATGTCGATGGTCTGA

Upstream 100 bases:

>100_bases
CCGGGCGGCGCCAACCTTTTTGAGATTAGAAGCCGCCGTCCACCGGCACTGCCGCGAACGCACGTAACATGACAGGACAC
ATTCGAAGGAGCGTGTCCTG

Downstream 100 bases:

>100_bases
TACGCGGCCTCGACCGTTTGCTGGCCGTATTGCCGCTCCAGCCGCCGCACGGTGAAGTGCGCGTGCGCGACCTGCTGGAA
GTGGTCGATGAACACCGTAT

Product: putative lipase/esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSWQSALACWYLRRQFRPETLKPGINVERARALTAKRVWSPRVPRGWHLRELYGQDDAPLRGEWIERADGSPVAGAGPIV
LYCHGGGYYFCSPRTHRSLVFGLATRANAAIFSLDYRLAPEHRFPAALNDTTAAYRRLIGGGIAPESIVIAGDSAGGGLA
LATLVALRDAGDPMPAGGLLFSPWTDLAATGASIHGNDGLDPMFSGPAIAPAGKIYLGDTPATHPYASPVYADLHGLPPL
FIMAGSTEVLLDDSQRVADNARAAGVDCELEVWQKMPHVWPIFAPFIPEGNRALDRAAGFVRRATSRAAQPSSAMSMV

Sequences:

>Translated_318_residues
MSWQSALACWYLRRQFRPETLKPGINVERARALTAKRVWSPRVPRGWHLRELYGQDDAPLRGEWIERADGSPVAGAGPIV
LYCHGGGYYFCSPRTHRSLVFGLATRANAAIFSLDYRLAPEHRFPAALNDTTAAYRRLIGGGIAPESIVIAGDSAGGGLA
LATLVALRDAGDPMPAGGLLFSPWTDLAATGASIHGNDGLDPMFSGPAIAPAGKIYLGDTPATHPYASPVYADLHGLPPL
FIMAGSTEVLLDDSQRVADNARAAGVDCELEVWQKMPHVWPIFAPFIPEGNRALDRAAGFVRRATSRAAQPSSAMSMV
>Mature_317_residues
SWQSALACWYLRRQFRPETLKPGINVERARALTAKRVWSPRVPRGWHLRELYGQDDAPLRGEWIERADGSPVAGAGPIVL
YCHGGGYYFCSPRTHRSLVFGLATRANAAIFSLDYRLAPEHRFPAALNDTTAAYRRLIGGGIAPESIVIAGDSAGGGLAL
ATLVALRDAGDPMPAGGLLFSPWTDLAATGASIHGNDGLDPMFSGPAIAPAGKIYLGDTPATHPYASPVYADLHGLPPLF
IMAGSTEVLLDDSQRVADNARAAGVDCELEVWQKMPHVWPIFAPFIPEGNRALDRAAGFVRRATSRAAQPSSAMSMV

Specific function: This protein removes the N-acetyl group from bialaphos as one of the final steps of the bialaphos biosynthetic pathway [H]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=293, Percent_Identity=26.962457337884, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115533412, Length=110, Percent_Identity=33.6363636363636, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI115533410, Length=110, Percent_Identity=33.6363636363636, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24656084, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24656076, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130169, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34099; Mature: 33968

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQSALACWYLRRQFRPETLKPGINVERARALTAKRVWSPRVPRGWHLRELYGQDDAPL
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCC
RGEWIERADGSPVAGAGPIVLYCHGGGYYFCSPRTHRSLVFGLATRANAAIFSLDYRLAP
CCHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCCHHEEEHHHCCCEEEEEEEEEECC
EHRFPAALNDTTAAYRRLIGGGIAPESIVIAGDSAGGGLALATLVALRDAGDPMPAGGLL
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEE
FSPWTDLAATGASIHGNDGLDPMFSGPAIAPAGKIYLGDTPATHPYASPVYADLHGLPPL
ECCCHHHHHCCCEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHCCCCCE
FIMAGSTEVLLDDSQRVADNARAAGVDCELEVWQKMPHVWPIFAPFIPEGNRALDRAAGF
EEEECCCEEEECCCHHHHCCCHHCCCCCCHHHHHCCCCCCEECCCCCCCCCHHHHHHHHH
VRRATSRAAQPSSAMSMV
HHHHHHHCCCCCHHHCCC
>Mature Secondary Structure 
SWQSALACWYLRRQFRPETLKPGINVERARALTAKRVWSPRVPRGWHLRELYGQDDAPL
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCC
RGEWIERADGSPVAGAGPIVLYCHGGGYYFCSPRTHRSLVFGLATRANAAIFSLDYRLAP
CCHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCCHHEEEHHHCCCEEEEEEEEEECC
EHRFPAALNDTTAAYRRLIGGGIAPESIVIAGDSAGGGLALATLVALRDAGDPMPAGGLL
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEE
FSPWTDLAATGASIHGNDGLDPMFSGPAIAPAGKIYLGDTPATHPYASPVYADLHGLPPL
ECCCHHHHHCCCEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHCCCCCE
FIMAGSTEVLLDDSQRVADNARAAGVDCELEVWQKMPHVWPIFAPFIPEGNRALDRAAGF
EEEECCCEEEECCCHHHHCCCHHCCCCCCHHHHHCCCCCCEECCCCCCCCCHHHHHHHHH
VRRATSRAAQPSSAMSMV
HHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2066341 [H]