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The map label for this gene is citE [H]

Identifier: 91781763

GI number: 91781763

Start: 419470

End: 420282

Strand: Direct

Name: citE [H]

Synonym: Bxe_A4082

Alternate gene names: 91781763

Gene position: 419470-420282 (Clockwise)

Preceding gene: 91781762

Following gene: 91781765

Centisome position: 8.57

GC content: 68.02

Gene sequence:

>813_bases
ATGGACGCACGCAGTTATCTGTTCGTGCCGGGCGACCGCCCCGAGCGCTTTGCCAAGGCGCTCGGCACCGGCGCCGACGC
GGTGGTGATCGACCTCGAAGACGCGGTTGTGCCGGCGGCGAAGCTTGCCGCGCGCGACGGCCTGCGGCGCTGGCTGCCGA
CAGTAGACCCGGCGCGGCTGATCGTACGCGTGAACGCGGTCGGCACGCCGTGGCATCTCGAAGATGTGGAGATGGTGCGC
GCTTCCGGCGTTTCCGTGATGATGCTGCCGAAGTCGGACAGCGCGCGGCAACTCGCCGAGGTCGCCGCGCGGCTGCCATC
AACGGTCCGGATCGTGGCGCTCATCGAAACCGTAGCGGGAGTGGTGGCGATGCGGGAAATTGCCGGCGTGCCGTCGGTTG
CGCGGCTTGCCTTCGGCACGGTGGATTTTTGCGGCGACGCCGGCATTGAAGGGCTCGGCGCGGAACTGGATTATGTGCGT
TCGCAGATGGTGATCGAATCGCGCTATGCGGGCCTCGCCGCGCCGATCGACGGTGTGACCCCGGAGCTCGATCATCTGGA
GCGTCTGACGGAACATGTGGCCGGCGCGCGGCGTTTCGGCTTCGGCGGCAAGCTGTGCATTCACCCGCGGCAGGTGGAGC
CGGTCAACCGCGGTTTCGCGCCCAGCGAGGACGAGCGCCGGTGGGCGGCGCGGGTTCTCGCGGCCTGTAGCGAGCATCCG
GAAGGCGCGTTCGCGGTGGACGGCAAACTGGTCGACCGGCCGGTTATCGAGCGGGCGAAGAAGATCGCGGAATTCGACAC
GCTCATCCACTAA

Upstream 100 bases:

>100_bases
AGCGTGCATCCGGCGGATCGCTATAGTTATTCCGAAACGTTTCAACGCGACTGGCACGCGAACTAGCAATCTTGCGCCGC
GTGCCGTTCAGGAGAACAAC

Downstream 100 bases:

>100_bases
CGACGTGGCGCGTTTGGGCCGGCGGCAAGGCGAGCCATGCCACGCTCACCGGCTCAGGTGGTCCAGCGCATCTCTCATAG
GCGAACTGGAGATCACCAGC

Product: citrate lyase

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MDARSYLFVPGDRPERFAKALGTGADAVVIDLEDAVVPAAKLAARDGLRRWLPTVDPARLIVRVNAVGTPWHLEDVEMVR
ASGVSVMMLPKSDSARQLAEVAARLPSTVRIVALIETVAGVVAMREIAGVPSVARLAFGTVDFCGDAGIEGLGAELDYVR
SQMVIESRYAGLAAPIDGVTPELDHLERLTEHVAGARRFGFGGKLCIHPRQVEPVNRGFAPSEDERRWAARVLAACSEHP
EGAFAVDGKLVDRPVIERAKKIAEFDTLIH

Sequences:

>Translated_270_residues
MDARSYLFVPGDRPERFAKALGTGADAVVIDLEDAVVPAAKLAARDGLRRWLPTVDPARLIVRVNAVGTPWHLEDVEMVR
ASGVSVMMLPKSDSARQLAEVAARLPSTVRIVALIETVAGVVAMREIAGVPSVARLAFGTVDFCGDAGIEGLGAELDYVR
SQMVIESRYAGLAAPIDGVTPELDHLERLTEHVAGARRFGFGGKLCIHPRQVEPVNRGFAPSEDERRWAARVLAACSEHP
EGAFAVDGKLVDRPVIERAKKIAEFDTLIH
>Mature_270_residues
MDARSYLFVPGDRPERFAKALGTGADAVVIDLEDAVVPAAKLAARDGLRRWLPTVDPARLIVRVNAVGTPWHLEDVEMVR
ASGVSVMMLPKSDSARQLAEVAARLPSTVRIVALIETVAGVVAMREIAGVPSVARLAFGTVDFCGDAGIEGLGAELDYVR
SQMVIESRYAGLAAPIDGVTPELDHLERLTEHVAGARRFGFGGKLCIHPRQVEPVNRGFAPSEDERRWAARVLAACSEHP
EGAFAVDGKLVDRPVIERAKKIAEFDTLIH

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=293, Percent_Identity=29.0102389078498, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI87081764, Length=277, Percent_Identity=30.3249097472924, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17557458, Length=294, Percent_Identity=29.5918367346939, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 29117; Mature: 29117

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARSYLFVPGDRPERFAKALGTGADAVVIDLEDAVVPAAKLAARDGLRRWLPTVDPARL
CCCCCEEEECCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEE
IVRVNAVGTPWHLEDVEMVRASGVSVMMLPKSDSARQLAEVAARLPSTVRIVALIETVAG
EEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
VVAMREIAGVPSVARLAFGTVDFCGDAGIEGLGAELDYVRSQMVIESRYAGLAAPIDGVT
HHHHHHHHCCCHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
PELDHLERLTEHVAGARRFGFGGKLCIHPRQVEPVNRGFAPSEDERRWAARVLAACSEHP
CCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
EGAFAVDGKLVDRPVIERAKKIAEFDTLIH
CCCEEECCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDARSYLFVPGDRPERFAKALGTGADAVVIDLEDAVVPAAKLAARDGLRRWLPTVDPARL
CCCCCEEEECCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEE
IVRVNAVGTPWHLEDVEMVRASGVSVMMLPKSDSARQLAEVAARLPSTVRIVALIETVAG
EEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
VVAMREIAGVPSVARLAFGTVDFCGDAGIEGLGAELDYVRSQMVIESRYAGLAAPIDGVT
HHHHHHHHCCCHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
PELDHLERLTEHVAGARRFGFGGKLCIHPRQVEPVNRGFAPSEDERRWAARVLAACSEHP
CCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
EGAFAVDGKLVDRPVIERAKKIAEFDTLIH
CCCEEECCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9457870 [H]