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The map label for this gene is purU [H]

Identifier: 91781734

GI number: 91781734

Start: 383922

End: 384791

Strand: Direct

Name: purU [H]

Synonym: Bxe_A4112

Alternate gene names: 91781734

Gene position: 383922-384791 (Clockwise)

Preceding gene: 91781733

Following gene: 91781738

Centisome position: 7.84

GC content: 62.07

Gene sequence:

>870_bases
ATGTCGACCGATCACAGCTTTATCCTCAAACTGTCGTGCGCCGACCGGCCCGGCATCGTTCACGCCGTGTCGGGCTTTCT
GTTCGAGCGTGGCAGCAATATTCTCGACTCCGCGCAGTTCGGCGACAGCCGCACCGGCGAGTTCTTCATGCGCGTGCATT
TCCAGCAGGTGGGCGGTGATCCGGGTCTGGAGGCGTTGCGCGCGTCGTTCGCGACGCTCGCAGAACAGTTCGGCATGCGC
TGGGAGTTGCACGACGCGTCGGTCAAGCCGCGCGTGGTGATCATGGTGTCGAAGATCGGCCATTGCCTGAACGATCTGCT
GTTCCGCTACCGCACCGGCCAGTTGGGTATCGAGATCGCGGCGATCATCTCGAACCACAAGGAGTTCTATCAACTCGCCG
CCAGCTACGACATTCCGTTCCATCACTTCCCGCTGATGGGCGCCACGCCCGAGGCGAAAGCCGCACAGGAAGCGCGCGTG
CTCGAAGTGATCGACGAGCATCAAGCCGACCTCGTGGTGCTCGCGCGCTACATGCAGATTCTGTCGCCGAAGCTGTGCGA
AGCGCTGGCCGGCCGCGCGATCAACATTCATCACTCGTTCCTGCCGAGCTTCAAGGGCGCGAAGCCGTATTACCAGGCAT
TCGACCGCGGTGTGAAGCTGATCGGCGCGACGGCTCATTACGTGACGACGGATCTCGACGAAGGTCCGATCATCGAGCAG
GAAGTGGAGCGCGTCGACCACAGCATGACGCCGGAGCAATTGACGGCGATCGGCCGCGACGTCGAATGCGTGACGCTCGC
CCGTGCGGTCAAGTGGCACGTTGAGCATCGCGTCGTGTTGAACGGCAGCAAGACGGTGGTGTTTCGCTAA

Upstream 100 bases:

>100_bases
CGCCGCAAATGGTGCGTGCGCAACGAACTTGCCGAAACACGTTGCATCGAACCATGCACACCGAACAAACCACGCATTGA
AGAAGGGAGTCACCCAGGTC

Downstream 100 bases:

>100_bases
AGCGGCTGATCGCTTTGCAGTAGTGGGTTGATGACAGATGGCGGCGGCCGGATCATTCCGGCGCCGCCATTTTGCCAACT
AGGGCCGCGCCGCGGACCGG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSTDHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSRTGEFFMRVHFQQVGGDPGLEALRASFATLAEQFGMR
WELHDASVKPRVVIMVSKIGHCLNDLLFRYRTGQLGIEIAAIISNHKEFYQLAASYDIPFHHFPLMGATPEAKAAQEARV
LEVIDEHQADLVVLARYMQILSPKLCEALAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPIIEQ
EVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRVVLNGSKTVVFR

Sequences:

>Translated_289_residues
MSTDHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSRTGEFFMRVHFQQVGGDPGLEALRASFATLAEQFGMR
WELHDASVKPRVVIMVSKIGHCLNDLLFRYRTGQLGIEIAAIISNHKEFYQLAASYDIPFHHFPLMGATPEAKAAQEARV
LEVIDEHQADLVVLARYMQILSPKLCEALAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPIIEQ
EVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRVVLNGSKTVVFR
>Mature_288_residues
STDHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSRTGEFFMRVHFQQVGGDPGLEALRASFATLAEQFGMRW
ELHDASVKPRVVIMVSKIGHCLNDLLFRYRTGQLGIEIAAIISNHKEFYQLAASYDIPFHHFPLMGATPEAKAAQEARVL
EVIDEHQADLVVLARYMQILSPKLCEALAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPIIEQE
VERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRVVLNGSKTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=167, Percent_Identity=31.1377245508982, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI209869995, Length=167, Percent_Identity=31.1377245508982, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI209869993, Length=167, Percent_Identity=31.1377245508982, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787483, Length=281, Percent_Identity=41.9928825622776, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=27.3684210526316, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=166, Percent_Identity=33.1325301204819, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32254; Mature: 32123

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSRTGEFFMRVHFQQVGGD
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHEEEHHHHCCC
PGLEALRASFATLAEQFGMRWELHDASVKPRVVIMVSKIGHCLNDLLFRYRTGQLGIEIA
CCHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHH
AIISNHKEFYQLAASYDIPFHHFPLMGATPEAKAAQEARVLEVIDEHQADLVVLARYMQI
HHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LSPKLCEALAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPIIEQ
HCHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCCCCCHH
EVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRVVLNGSKTVVFR
HHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCEEEEEC
>Mature Secondary Structure 
STDHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSRTGEFFMRVHFQQVGGD
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHEEEHHHHCCC
PGLEALRASFATLAEQFGMRWELHDASVKPRVVIMVSKIGHCLNDLLFRYRTGQLGIEIA
CCHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHH
AIISNHKEFYQLAASYDIPFHHFPLMGATPEAKAAQEARVLEVIDEHQADLVVLARYMQI
HHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LSPKLCEALAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPIIEQ
HCHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCCCCCHH
EVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRVVLNGSKTVVFR
HHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]