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The map label for this gene is rhtB [C]

Identifier: 91781732

GI number: 91781732

Start: 382283

End: 382897

Strand: Direct

Name: rhtB [C]

Synonym: Bxe_A4114

Alternate gene names: 91781732

Gene position: 382283-382897 (Clockwise)

Preceding gene: 91781731

Following gene: 91781733

Centisome position: 7.81

GC content: 65.37

Gene sequence:

>615_bases
ATGCCTGACTTCCTGCTGTTTCTCGCTACCTCGATTGCGATCACCGTGGCACCCGGCCCGGACAATCTGCAAGTGCTCGC
GCGCGGTATCTCGCAGGGCCGCGCGGCAGGGTTCGTGGCGGCGCTCGGCTTTGCGGCCGGCATCACGTTTCACACCACGC
TTGCCGCGCTCGGCGTGGCGGCGCTGCTGCGTTCCTCGCCGACGGCCTTTACGGCGATCAAGCTCGCCGGCGCCGCTTAT
CTGATCTGGATCGGCATCAAGGCTCTGCGCAGCCAGGGGCTCGCAACCGCGCACGAGCGCGCGCCGCAGCCGTTGATGGC
GGTGTTTCGCCAAAGCGTGCTCGGCAATCTGCTGAATCCGAAGGTGACGTTGTTCTTCGTCGTGTTTCTGCCGCAGTTCG
TGCAGCCGAACGGCACGCAAAGCGTCACGCTGCAGATGCTCGAACTCGGCGTGCTGTTCATGTTGCAGACGGTGGTGGTG
TTTTCGCTGTTCGGCGTCTGCGCGGGGATGGTGGGCGGATATCTGAAGCGCCGGCCGCGCGTGGGCGTGTGGCTCGATCG
GCTCGCCGGTGCAACGTTCATCGCCATCGGGATTCGCGTCGCGTTGCGCGATTGA

Upstream 100 bases:

>100_bases
GGCTGGCGCTCTATACGGTGACCGGATTCGAGGGTCACTGATCACCGGCAGCCAACCCGCTGCGAACTTCTTCTTTCTGT
CCATCGAGAAGGTCAAAAGC

Downstream 100 bases:

>100_bases
GGCGATCGACGCGGCGCTCGGCGCTCAGCGCCAGGTGCTCACCGGGCAGGACCGCGAACCGTTTGGAGCTTGACATGACT
TTGCCTTGCATCACCGCCGC

Product: amino acid efflux pump, RhtB family protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MPDFLLFLATSIAITVAPGPDNLQVLARGISQGRAAGFVAALGFAAGITFHTTLAALGVAALLRSSPTAFTAIKLAGAAY
LIWIGIKALRSQGLATAHERAPQPLMAVFRQSVLGNLLNPKVTLFFVVFLPQFVQPNGTQSVTLQMLELGVLFMLQTVVV
FSLFGVCAGMVGGYLKRRPRVGVWLDRLAGATFIAIGIRVALRD

Sequences:

>Translated_204_residues
MPDFLLFLATSIAITVAPGPDNLQVLARGISQGRAAGFVAALGFAAGITFHTTLAALGVAALLRSSPTAFTAIKLAGAAY
LIWIGIKALRSQGLATAHERAPQPLMAVFRQSVLGNLLNPKVTLFFVVFLPQFVQPNGTQSVTLQMLELGVLFMLQTVVV
FSLFGVCAGMVGGYLKRRPRVGVWLDRLAGATFIAIGIRVALRD
>Mature_203_residues
PDFLLFLATSIAITVAPGPDNLQVLARGISQGRAAGFVAALGFAAGITFHTTLAALGVAALLRSSPTAFTAIKLAGAAYL
IWIGIKALRSQGLATAHERAPQPLMAVFRQSVLGNLLNPKVTLFFVVFLPQFVQPNGTQSVTLQMLELGVLFMLQTVVVF
SLFGVCAGMVGGYLKRRPRVGVWLDRLAGATFIAIGIRVALRD

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=192, Percent_Identity=29.1666666666667, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788099, Length=201, Percent_Identity=27.8606965174129, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1786522, Length=113, Percent_Identity=30.9734513274336, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI48994978, Length=208, Percent_Identity=29.8076923076923, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21608; Mature: 21477

Theoretical pI: Translated: 11.88; Mature: 11.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFLLFLATSIAITVAPGPDNLQVLARGISQGRAAGFVAALGFAAGITFHTTLAALGVA
CCHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLRSSPTAFTAIKLAGAAYLIWIGIKALRSQGLATAHERAPQPLMAVFRQSVLGNLLNP
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCC
KVTLFFVVFLPQFVQPNGTQSVTLQMLELGVLFMLQTVVVFSLFGVCAGMVGGYLKRRPR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VGVWLDRLAGATFIAIGIRVALRD
CHHHHHHHCCHHHHHHHHEEEECC
>Mature Secondary Structure 
PDFLLFLATSIAITVAPGPDNLQVLARGISQGRAAGFVAALGFAAGITFHTTLAALGVA
CHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLRSSPTAFTAIKLAGAAYLIWIGIKALRSQGLATAHERAPQPLMAVFRQSVLGNLLNP
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCC
KVTLFFVVFLPQFVQPNGTQSVTLQMLELGVLFMLQTVVVFSLFGVCAGMVGGYLKRRPR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VGVWLDRLAGATFIAIGIRVALRD
CHHHHHHHCCHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]