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The map label for this gene is ptsN [H]

Identifier: 91781722

GI number: 91781722

Start: 372648

End: 373163

Strand: Reverse

Name: ptsN [H]

Synonym: Bxe_A4124

Alternate gene names: 91781722

Gene position: 373163-372648 (Counterclockwise)

Preceding gene: 91781723

Following gene: 91781721

Centisome position: 7.62

GC content: 59.88

Gene sequence:

>516_bases
ATGGAACGCCACTCAAACGCCCCAGCGAGGAGAACCCAGGCCACGTTTTCGCCTGTCAACATGAATCGTTTAGCCAAATT
TCTTCCCCTCGAGAACGTCGTCGTCGGACTATCGGTCACCAGCAAGAAACGCGTGTTCGAGCAAGCGGGCCTGATCTTCG
AGAACCAGAACGGCATAGCCCGTAGCACGGTCACTGACAATCTGTTCGCGCGTGAGCGCCTCGGATCGACAGGCCTTGGC
GAAGGCGTGGCGATTCCGCATGGCCGGATCAAAGGCTTGAAGCAGCCGCTCGCCGCGTTCGTCCGCCTTGCCGAACCCAT
CCCCTTCGAATCGCCCGACGGTCAGCCGGTCTCGCTGCTCATCTTCCTGCTCGTGCCTGAACAGGCCACTCAGCAACACC
TCGAAATCCTTTCGGAAATCGCCCAGTTGCTGTCCGATCGCGAAACCCGCGAGCGCCTGCACACGGAACAGGACCGCGAA
ACGTTGCATCGCCTGCTCACTCAGTGGCAACCTTGA

Upstream 100 bases:

>100_bases
TTCCGGTTACCATCGGGGTCAAGTTAGCTCAAGCGGAAATCGGTCGGCCGGAGTCCTGCGCTTTCGGACTCCCACGCACG
TCGCCGTGAGCATCAAACGA

Downstream 100 bases:

>100_bases
TGCATCGGCGGTTATCCGCACGCCGGTTATCCGCCCGGTCATCGGCCCGCATGCGGAGACAGTCCGGCAACGCAAACGGC
CCGCGCTAGCGCGGCCGCCG

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MERHSNAPARRTQATFSPVNMNRLAKFLPLENVVVGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLG
EGVAIPHGRIKGLKQPLAAFVRLAEPIPFESPDGQPVSLLIFLLVPEQATQQHLEILSEIAQLLSDRETRERLHTEQDRE
TLHRLLTQWQP

Sequences:

>Translated_171_residues
MERHSNAPARRTQATFSPVNMNRLAKFLPLENVVVGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLG
EGVAIPHGRIKGLKQPLAAFVRLAEPIPFESPDGQPVSLLIFLLVPEQATQQHLEILSEIAQLLSDRETRERLHTEQDRE
TLHRLLTQWQP
>Mature_171_residues
MERHSNAPARRTQATFSPVNMNRLAKFLPLENVVVGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLG
EGVAIPHGRIKGLKQPLAAFVRLAEPIPFESPDGQPVSLLIFLLVPEQATQQHLEILSEIAQLLSDRETRERLHTEQDRE
TLHRLLTQWQP

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=131, Percent_Identity=38.1679389312977, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI2367327, Length=119, Percent_Identity=32.7731092436975, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786951, Length=92, Percent_Identity=35.8695652173913, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1788726, Length=145, Percent_Identity=29.6551724137931, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 19191; Mature: 19191

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERHSNAPARRTQATFSPVNMNRLAKFLPLENVVVGLSVTSKKRVFEQAGLIFENQNGIA
CCCCCCCCCHHHHCCCCCCCHHHHHHHCCHHHEEEECCCHHHHHHHHHHCCEEECCCCCC
RSTVTDNLFARERLGSTGLGEGVAIPHGRIKGLKQPLAAFVRLAEPIPFESPDGQPVSLL
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
IFLLVPEQATQQHLEILSEIAQLLSDRETRERLHTEQDRETLHRLLTQWQP
HEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERHSNAPARRTQATFSPVNMNRLAKFLPLENVVVGLSVTSKKRVFEQAGLIFENQNGIA
CCCCCCCCCHHHHCCCCCCCHHHHHHHCCHHHEEEECCCHHHHHHHHHHCCEEECCCCCC
RSTVTDNLFARERLGSTGLGEGVAIPHGRIKGLKQPLAAFVRLAEPIPFESPDGQPVSLL
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
IFLLVPEQATQQHLEILSEIAQLLSDRETRERLHTEQDRETLHRLLTQWQP
HEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]