Click here to switch to the map view.

The map label for this gene is prs [H]

Identifier: 91781713

GI number: 91781713

Start: 363214

End: 364170

Strand: Reverse

Name: prs [H]

Synonym: Bxe_A4133

Alternate gene names: 91781713

Gene position: 364170-363214 (Counterclockwise)

Preceding gene: 91781714

Following gene: 91781712

Centisome position: 7.44

GC content: 59.87

Gene sequence:

>957_bases
ATGAGCAGCCATGACGGCCTGATGGTTTTTACTGGCAACGCAAATCCCGCGCTTGCACAGGAAGTCGTCAAAATCCTCGG
TATTCCCCTCGGCAAAGCAATGGTCAGCCGTTTCTCGGACGGTGAAATCCAGGTCGAGATTCAGGAAAACGTGCGTGGCA
AGGATGTCTTCGTCCTGCAGTCCACGTGCGCACCGGCGAACGACAATCTGATGGAACTGATGATCATGGTCGATGCGCTC
AAGCGCGCATCCGCAGGCCGGATCACCGCAGCCATCCCCTACTTCGGTTATGCCCGTCAAGATCGCCGCCCGCGTTCCGC
GCGCGTCGCCATCTCGGCGAAGATCGTGGCGAACATGCTGGAAATCGCCGGCGTCGAGCGGATCATTACGATGGATCTGC
ACGCCGACCAGATTCAAGGCTTCTTCGACATTCCGGTCGACAACATCTACGCTACGCCCGTGCTGCTCGGCGATCTGCGC
AAGCAGAACTACGAGAATCTGCTGGTCGTTTCGCCGGACGTCGGCGGCGTGGTGCGTGCCCGTGCATTGGCCAAACAGCT
CAATTGCGATCTCGCGATCATCGACAAACGCCGCCCGAAGGCGAACGTTGCCGAAGTGATGAACATCATCGGTGAAGTCG
AAGGTCGTACCTGCGTGATCATGGACGACATGGTCGATACTGCCGGCACGCTCTGCAAGGCAGCTCAGGTTCTGAAGGAA
CGCGGCGCGAAACAGGTGTTCGCTTATGCCACTCACCCGGTTCTGTCGGGCGGCGCGGGCGAGCGTATCGCTGCCTCCGC
ACTCGACGAACTCGTTGTCACGGACACGATCCCGCTCGGCGAAGAAGCCCGCTCGTGCGCGAAGATCCGCTCGTTGACCA
GCGCTGGCCTGCTGGCCGAAACGTTCTCGCGGATTCGCCGCGGCGATTCGGTGATGTCGCTGTTCGCTGAAAGTTAA

Upstream 100 bases:

>100_bases
GGCACCGGATTTTGATTCCGGCATGCAAAGGTTCGAATCCTTTCTCCCCTGCCAAAATTTCTCGCATTTCCCCTCGCCCC
CAGCCTGAAGCAGGTGCACG

Downstream 100 bases:

>100_bases
GTATTTGCGAAGGCGCAGCGCGCCGCTTCAGGCGGCACACCGCGCTTTTGCACAATCGGCATGAACGAACGAAGGTTCGT
GCCGCAGCTCTGGGGCCTCA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSSHDGLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQSTCAPANDNLMELMIMVDAL
KRASAGRITAAIPYFGYARQDRRPRSARVAISAKIVANMLEIAGVERIITMDLHADQIQGFFDIPVDNIYATPVLLGDLR
KQNYENLLVVSPDVGGVVRARALAKQLNCDLAIIDKRRPKANVAEVMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKE
RGAKQVFAYATHPVLSGGAGERIAASALDELVVTDTIPLGEEARSCAKIRSLTSAGLLAETFSRIRRGDSVMSLFAES

Sequences:

>Translated_318_residues
MSSHDGLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQSTCAPANDNLMELMIMVDAL
KRASAGRITAAIPYFGYARQDRRPRSARVAISAKIVANMLEIAGVERIITMDLHADQIQGFFDIPVDNIYATPVLLGDLR
KQNYENLLVVSPDVGGVVRARALAKQLNCDLAIIDKRRPKANVAEVMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKE
RGAKQVFAYATHPVLSGGAGERIAASALDELVVTDTIPLGEEARSCAKIRSLTSAGLLAETFSRIRRGDSVMSLFAES
>Mature_317_residues
SSHDGLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQSTCAPANDNLMELMIMVDALK
RASAGRITAAIPYFGYARQDRRPRSARVAISAKIVANMLEIAGVERIITMDLHADQIQGFFDIPVDNIYATPVLLGDLRK
QNYENLLVVSPDVGGVVRARALAKQLNCDLAIIDKRRPKANVAEVMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKER
GAKQVFAYATHPVLSGGAGERIAASALDELVVTDTIPLGEEARSCAKIRSLTSAGLLAETFSRIRRGDSVMSLFAES

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=47.7707006369427, Blast_Score=305, Evalue=5e-83,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=48.0891719745223, Blast_Score=304, Evalue=8e-83,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=47.7707006369427, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI84875539, Length=316, Percent_Identity=47.1518987341772, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI4506133, Length=346, Percent_Identity=36.7052023121387, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=37.3913043478261, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=36.9127516778523, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=36.9127516778523, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=36.9127516778523, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=36.9127516778523, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1787458, Length=310, Percent_Identity=62.9032258064516, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=46.8152866242038, Blast_Score=302, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=47.2668810289389, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=46.8152866242038, Blast_Score=301, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=46.8152866242038, Blast_Score=301, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=33.6283185840708, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319403, Length=318, Percent_Identity=43.7106918238994, Blast_Score=266, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6321776, Length=313, Percent_Identity=44.408945686901, Blast_Score=265, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6320946, Length=319, Percent_Identity=42.9467084639498, Blast_Score=265, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=43.3673469387755, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=108, Percent_Identity=39.8148148148148, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355239, Length=313, Percent_Identity=48.2428115015974, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI45551540, Length=335, Percent_Identity=45.0746268656716, Blast_Score=286, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24651458, Length=359, Percent_Identity=33.7047353760446, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24651456, Length=359, Percent_Identity=33.7047353760446, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI281362873, Length=359, Percent_Identity=33.7047353760446, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24651454, Length=359, Percent_Identity=33.7047353760446, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24651462, Length=207, Percent_Identity=38.6473429951691, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24651464, Length=207, Percent_Identity=38.6473429951691, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45552010, Length=207, Percent_Identity=38.6473429951691, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34287; Mature: 34156

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHDGLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQ
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEECCCCCCEEEEEE
STCAPANDNLMELMIMVDALKRASAGRITAAIPYFGYARQDRRPRSARVAISAKIVANML
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHEEEEHHHHHHHHH
EIAGVERIITMDLHADQIQGFFDIPVDNIYATPVLLGDLRKQNYENLLVVSPDVGGVVRA
HHHCCCEEEEEECCHHHHCCEEECCCCCEEECHHHHHHHHHCCCCCEEEECCCCCHHHHH
RALAKQLNCDLAIIDKRRPKANVAEVMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKE
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
RGAKQVFAYATHPVLSGGAGERIAASALDELVVTDTIPLGEEARSCAKIRSLTSAGLLAE
CCCHHEEHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
TFSRIRRGDSVMSLFAES
HHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
SSHDGLMVFTGNANPALAQEVVKILGIPLGKAMVSRFSDGEIQVEIQENVRGKDVFVLQ
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEECCCCCCEEEEEE
STCAPANDNLMELMIMVDALKRASAGRITAAIPYFGYARQDRRPRSARVAISAKIVANML
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHEEEEHHHHHHHHH
EIAGVERIITMDLHADQIQGFFDIPVDNIYATPVLLGDLRKQNYENLLVVSPDVGGVVRA
HHHCCCEEEEEECCHHHHCCEEECCCCCEEECHHHHHHHHHCCCCCEEEECCCCCHHHHH
RALAKQLNCDLAIIDKRRPKANVAEVMNIIGEVEGRTCVIMDDMVDTAGTLCKAAQVLKE
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
RGAKQVFAYATHPVLSGGAGERIAASALDELVVTDTIPLGEEARSCAKIRSLTSAGLLAE
CCCHHEEHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
TFSRIRRGDSVMSLFAES
HHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]