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The map label for this gene is manX [C]

Identifier: 91781660

GI number: 91781660

Start: 311956

End: 312516

Strand: Direct

Name: manX [C]

Synonym: Bxe_A4186

Alternate gene names: 91781660

Gene position: 311956-312516 (Clockwise)

Preceding gene: 91781659

Following gene: 91781661

Centisome position: 6.37

GC content: 65.95

Gene sequence:

>561_bases
TTGGGGCCGTTCTTTTGCAGTAAGGCTGACATGGCAGGCATTCTGATCATTGCGCACGCTCCGTTCGCCACCGCGTTGCG
CGATTGCATTTCGCACATCTATGGTGGCTTGCCGGCTCGCATCGGCGTAATCGACGTATCGCCGGATTGCGACCCCGCAC
AGCAGGTCGCGTTCGCGCATGCCGAGGTCGAGCGTCTCAAGGAAGAAAACGGCGCACTTGTGCTCACCGATATGTTCGGC
GCCACGCCGGCGAATATCGCCGGCCGGCTTGCTTCGGTGCCGAATGTGCGCGTGCTGTGTGGCGTCAACCTGCCGATGCT
GGTGCGCGCGGTCTGTTATCGCGCCACACCGCTCGACACACTGGTCGACAAGGCGCTTGCGGGCGCCACCAAGGGCGTGC
ACGCAATCGGACCGGCCACGCCGGCGCCCGTCGCGCCCACTGCGGAGTCCGGCGACTGTCTGCCCGCACTGCCGCCCGAC
GCGGCATCCGGCGGCGACTGTTTACCGGCCTTGCCGGCCGACGCCGATCTGACGCAGAAAGCCGGTCCAGCCGGTCTGTA
A

Upstream 100 bases:

>100_bases
CCCGCGCGCGGCGGATCGCTCGTCGCATCATGCGTTCCGGCCCTCATGGCCCACGGCCGGCCGAGGGTGCAGACGAACCG
GGCCGGGTCCCGGTCCACGG

Downstream 100 bases:

>100_bases
TCAGAGAGCCGCACGGCCGGCTGCCAGGTTTCAAACGCATTGCCACTGGTTTGCAGATTCACTTTTCACAACAACACTTA
CCCGCGCTTCGGACCATTAC

Product: putative sugar transport PTS system IIA component

Products: Protein Histidine; Sugar Phosphate.; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: IIA Component Of Sugar Transport PTS System; PTS System IIA Component; PTS System Fructose-Specific IIA Component; PTS System Fructose Subfamily Transporter Subunit IIA; Protein-N P-Phosphohistidine-Sugar Phosphotransferase; Sugar Transport PTS System IIA Component; PTS System Fructose IIA Component Family Protein; Pts System Fructose Subfamily IIa Component; PTS System Fructose IIA Component; IIa Component Of Sugar Transport PTS System; PTS System IIAB Component; PTS System Transporter Subunit IIA; Pts System IIa Component; Pentitol Phosphotransferase IIA Component; Phosphotransferase Mannose/Fructose-Specific Component IIA; PTS System IIA Component Domain Protein

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MGPFFCSKADMAGILIIAHAPFATALRDCISHIYGGLPARIGVIDVSPDCDPAQQVAFAHAEVERLKEENGALVLTDMFG
ATPANIAGRLASVPNVRVLCGVNLPMLVRAVCYRATPLDTLVDKALAGATKGVHAIGPATPAPVAPTAESGDCLPALPPD
AASGGDCLPALPADADLTQKAGPAGL

Sequences:

>Translated_186_residues
MGPFFCSKADMAGILIIAHAPFATALRDCISHIYGGLPARIGVIDVSPDCDPAQQVAFAHAEVERLKEENGALVLTDMFG
ATPANIAGRLASVPNVRVLCGVNLPMLVRAVCYRATPLDTLVDKALAGATKGVHAIGPATPAPVAPTAESGDCLPALPPD
AASGGDCLPALPADADLTQKAGPAGL
>Mature_185_residues
GPFFCSKADMAGILIIAHAPFATALRDCISHIYGGLPARIGVIDVSPDCDPAQQVAFAHAEVERLKEENGALVLTDMFGA
TPANIAGRLASVPNVRVLCGVNLPMLVRAVCYRATPLDTLVDKALAGATKGVHAIGPATPAPVAPTAESGDCLPALPPDA
ASGGDCLPALPADADLTQKAGPAGL

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 18813; Mature: 18682

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPFFCSKADMAGILIIAHAPFATALRDCISHIYGGLPARIGVIDVSPDCDPAQQVAFAH
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
AEVERLKEENGALVLTDMFGATPANIAGRLASVPNVRVLCGVNLPMLVRAVCYRATPLDT
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHH
LVDKALAGATKGVHAIGPATPAPVAPTAESGDCLPALPPDAASGGDCLPALPADADLTQK
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
AGPAGL
CCCCCC
>Mature Secondary Structure 
GPFFCSKADMAGILIIAHAPFATALRDCISHIYGGLPARIGVIDVSPDCDPAQQVAFAH
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
AEVERLKEENGALVLTDMFGATPANIAGRLASVPNVRVLCGVNLPMLVRAVCYRATPLDT
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHH
LVDKALAGATKGVHAIGPATPAPVAPTAESGDCLPALPPDAASGGDCLPALPADADLTQK
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
AGPAGL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA