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The map label for this gene is gshB [H]

Identifier: 91781659

GI number: 91781659

Start: 310677

End: 311633

Strand: Direct

Name: gshB [H]

Synonym: Bxe_A4187

Alternate gene names: 91781659

Gene position: 310677-311633 (Clockwise)

Preceding gene: 91781658

Following gene: 91781660

Centisome position: 6.35

GC content: 63.95

Gene sequence:

>957_bases
ATGGACATTCTTTTCATCGCCGATCCGCTCACGCAATTCAAGATCTACAAAGACTCGACCTACGCGATGATGGCCGAGGC
GGCGCGCCGGGCTCATGTGGTGTACACGTGCGAGCCGCAGCATCTTGCGTGGACGGGCGGCGAGGTGGAGGCGAACGTGC
AGCGCGTGGAGATCGTCGGCGACGGTGCCGATCTGCAGCGCGACGTCTGGTACAGCGCCGAAGCGGCCGCGCCGCGTTCG
CTCAGGAGTTTCAGCGCGGTGGTGATGCGCAAAGACCCGCCGTTCGACATGGAATACGTCACGTCGACATGGCTGCTGGA
AATGGCGGAACGCGCCGGCGCGCGCATTTTTAACAAGCCGCAGGCGATTCGCGACCACTCCGAAAAGCTCGCCATCGGCG
AATTCGCGCAGTTCGTCACGCCTACGCTCGTCACGCGCGACGCGACCCGCCTGCGCGCCTTTCACGAAGAACACGGCGAC
GTGATCCTGAAGCCGCTCGACGGCATGGGCGGCATGGGCGTGTTCCGCGTCAAGGCGGACGGCATGAACCTCGGTTCGAT
CATCGAAATGCTGAGCCACGACGGCACGCGTTCGGTGATGGCGCAGAAATTCATTCCCGAGATCAAGGAGGGCGACAAGC
GCATTCTGCTGATCGGCGGCGAAGCCGTGCCGTATTCGCTCGCGCGTATTCCTCAGGGCAATGAAGTGCGCGGCAATCTC
GCGGCCGGCGGCCTCGGCATGGCGCGTCCGCTGACGGAGCACGACCGCAAGATCGCCGCTACGCTGGCGCCGGTGCTGGC
TGCGCGCGGTTTGCTGCTGGTCGGACTGGATGCGATCGGTGACTGGCTGACCGAAGTGAATGTGACGAGTCCGACATGTT
TCCGCGAGATCATGGATCAAACCGGCTTCGACGTTGCCGCCATGTTCATCGACGCGCTGGAGCGCGCGGCGGGTTGA

Upstream 100 bases:

>100_bases
CACCCTGGGTGGGCGATGGCGGCATGCGTGCCGCCATCGCCATTTCCAACGACTATATTTACGATCAATGCCAAGCCCAT
CAGGGCGCACCGGGACCTTC

Downstream 100 bases:

>100_bases
TGATGCGGTCCGACGGGTGCTCTTGAATGGCCGCCATGATCCCCAAACTGTGTATTAAATCCGCATCAGGCGGCGCGGTG
GCTTTGGCGTGAAAATGAGC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MDILFIADPLTQFKIYKDSTYAMMAEAARRAHVVYTCEPQHLAWTGGEVEANVQRVEIVGDGADLQRDVWYSAEAAAPRS
LRSFSAVVMRKDPPFDMEYVTSTWLLEMAERAGARIFNKPQAIRDHSEKLAIGEFAQFVTPTLVTRDATRLRAFHEEHGD
VILKPLDGMGGMGVFRVKADGMNLGSIIEMLSHDGTRSVMAQKFIPEIKEGDKRILLIGGEAVPYSLARIPQGNEVRGNL
AAGGLGMARPLTEHDRKIAATLAPVLAARGLLLVGLDAIGDWLTEVNVTSPTCFREIMDQTGFDVAAMFIDALERAAG

Sequences:

>Translated_318_residues
MDILFIADPLTQFKIYKDSTYAMMAEAARRAHVVYTCEPQHLAWTGGEVEANVQRVEIVGDGADLQRDVWYSAEAAAPRS
LRSFSAVVMRKDPPFDMEYVTSTWLLEMAERAGARIFNKPQAIRDHSEKLAIGEFAQFVTPTLVTRDATRLRAFHEEHGD
VILKPLDGMGGMGVFRVKADGMNLGSIIEMLSHDGTRSVMAQKFIPEIKEGDKRILLIGGEAVPYSLARIPQGNEVRGNL
AAGGLGMARPLTEHDRKIAATLAPVLAARGLLLVGLDAIGDWLTEVNVTSPTCFREIMDQTGFDVAAMFIDALERAAG
>Mature_318_residues
MDILFIADPLTQFKIYKDSTYAMMAEAARRAHVVYTCEPQHLAWTGGEVEANVQRVEIVGDGADLQRDVWYSAEAAAPRS
LRSFSAVVMRKDPPFDMEYVTSTWLLEMAERAGARIFNKPQAIRDHSEKLAIGEFAQFVTPTLVTRDATRLRAFHEEHGD
VILKPLDGMGGMGVFRVKADGMNLGSIIEMLSHDGTRSVMAQKFIPEIKEGDKRILLIGGEAVPYSLARIPQGNEVRGNL
AAGGLGMARPLTEHDRKIAATLAPVLAARGLLLVGLDAIGDWLTEVNVTSPTCFREIMDQTGFDVAAMFIDALERAAG

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=310, Percent_Identity=48.3870967741936, Blast_Score=301, Evalue=3e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34862; Mature: 34862

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILFIADPLTQFKIYKDSTYAMMAEAARRAHVVYTCEPQHLAWTGGEVEANVQRVEIVG
CCEEEEECCCCCEEEECCCHHHHHHHHHHHEEEEEEECCCEEEECCCCEECCEEEEEEEE
DGADLQRDVWYSAEAAAPRSLRSFSAVVMRKDPPFDMEYVTSTWLLEMAERAGARIFNKP
CCCCCCHHHCCCCCCCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHCCCC
QAIRDHSEKLAIGEFAQFVTPTLVTRDATRLRAFHEEHGDVILKPLDGMGGMGVFRVKAD
HHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEC
GMNLGSIIEMLSHDGTRSVMAQKFIPEIKEGDKRILLIGGEAVPYSLARIPQGNEVRGNL
CCCHHHHHHHHHCCCHHHHHHHHHCHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCCEE
AAGGLGMARPLTEHDRKIAATLAPVLAARGLLLVGLDAIGDWLTEVNVTSPTCFREIMDQ
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHCCCCCHHHHHHHHHH
TGFDVAAMFIDALERAAG
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDILFIADPLTQFKIYKDSTYAMMAEAARRAHVVYTCEPQHLAWTGGEVEANVQRVEIVG
CCEEEEECCCCCEEEECCCHHHHHHHHHHHEEEEEEECCCEEEECCCCEECCEEEEEEEE
DGADLQRDVWYSAEAAAPRSLRSFSAVVMRKDPPFDMEYVTSTWLLEMAERAGARIFNKP
CCCCCCHHHCCCCCCCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHCCCC
QAIRDHSEKLAIGEFAQFVTPTLVTRDATRLRAFHEEHGDVILKPLDGMGGMGVFRVKAD
HHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEC
GMNLGSIIEMLSHDGTRSVMAQKFIPEIKEGDKRILLIGGEAVPYSLARIPQGNEVRGNL
CCCHHHHHHHHHCCCHHHHHHHHHCHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCCEE
AAGGLGMARPLTEHDRKIAATLAPVLAARGLLLVGLDAIGDWLTEVNVTSPTCFREIMDQ
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHCCCCCHHHHHHHHHH
TGFDVAAMFIDALERAAG
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]