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The map label for this gene is metH [H]

Identifier: 91781606

GI number: 91781606

Start: 258714

End: 259793

Strand: Reverse

Name: metH [H]

Synonym: Bxe_A4240

Alternate gene names: 91781606

Gene position: 259793-258714 (Counterclockwise)

Preceding gene: 91781607

Following gene: 91781605

Centisome position: 5.31

GC content: 62.96

Gene sequence:

>1080_bases
ATGAACCAGCCCGCTCAAACCGCCACGCCAGTCCGTCCCGACGCCGCCTACACGCGCGGCGCGGCGCTGCCCGCGCTGCT
CAAGTCGCGCATCCTGATTCTGGACGGCGCAATGGGCACGATGATCCAGCGCTACAAGCTCGACGAAGCCCGTTATCGCG
GCGAGCGCTTCAAGGACTACGGGCGCGACATCAAAGGCAACAACGAGTTGCTGTCCATCACGCAGCCGCAGATCATCAGC
GAGATCCATGAGCAGTATCTGGCGGCGGGCGCGGACATCGTCGAAACCAATACGTTCGGTGCGACCACGGTCGCTCAGGC
GGACTACGGTATGGAAGCGCTCGCGGTCGAGATGAATCTCGAGTCCGCAAAACTGGCGCGTGCCGCGTGCGACAAGTATT
CGACGCCCGACAAGCCGCGCTTTGTCGCCGGCGCGATCGGACCGACGCCGAAGACCGCGAGCATTTCCCCCGACGTGAAC
GATCCGGGCGCCCGCAACGTGACTTTCGACGAACTGCGCGCGGCCTACTACGAGCAGGCCAGGGCGTTGCTCGACGGCGG
CGTCGATCTGTTCCTCGTGGAGACCATTTTCGACACGCTCAATGCGAAGGCAGCGCTGTTCGCACTGGACGAACTGTTCG
AAGATACCGGCGAGCGGCTGCCGATCATGATCTCCGGCACCGTCACCGACGCCTCGGGACGCATTCTCTCGGGCCAGACA
GTCGAAGCCTTCTGGAACTCGCTGCGGCACGCCAAACCGCTCACCTTCGGTTTGAACTGCGCGCTGGGGGCGGCTTTGAT
GCGCCCGTATATCGCCGAGCTGGCCAAGCTGTGCGACACGTATGTGTCGTGCTATCCGAACGCCGGCTTGCCGAATCCGA
TGAGCGATACGGGCTTTGACGAATTGCCCGCGGATACCTCTGGTTTGCTGAAGGAATTCGCACAGGCCGGGCTCGTGAAT
ATTGCCGGCGGCTGCTGTGGGACGACGCCGGAGCATATTGCGGCGATTGCGCAGGCGCTGGCTGGCGTGAAGCCGCGCGT
GTGGGCAAACTATCGGGAAACCGATACTGAAACAGTTTGA

Upstream 100 bases:

>100_bases
CATAGGCGAGGCGGCCGTACCGCCCGCGCCGGGCGATTTCGCCCGGCAGCGGCTACAATGACAGGTTTCCCGAAAAAGCC
GGCGCAAGCCCTCGTCCGCC

Downstream 100 bases:

>100_bases
TCGCAGCGTCTACCTGGATGGGAATCGAGCAATGGCCGGCGTCACCAAACTGACGATTGCGGGTTTCAAGTCGATACGTG
AGTTGCGCGATTTCGAGTTG

Product: methionine synthase (B12-dependent)

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNQPAQTATPVRPDAAYTRGAALPALLKSRILILDGAMGTMIQRYKLDEARYRGERFKDYGRDIKGNNELLSITQPQIIS
EIHEQYLAAGADIVETNTFGATTVAQADYGMEALAVEMNLESAKLARAACDKYSTPDKPRFVAGAIGPTPKTASISPDVN
DPGARNVTFDELRAAYYEQARALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMISGTVTDASGRILSGQT
VEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDELPADTSGLLKEFAQAGLVN
IAGGCCGTTPEHIAAIAQALAGVKPRVWANYRETDTETV

Sequences:

>Translated_359_residues
MNQPAQTATPVRPDAAYTRGAALPALLKSRILILDGAMGTMIQRYKLDEARYRGERFKDYGRDIKGNNELLSITQPQIIS
EIHEQYLAAGADIVETNTFGATTVAQADYGMEALAVEMNLESAKLARAACDKYSTPDKPRFVAGAIGPTPKTASISPDVN
DPGARNVTFDELRAAYYEQARALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMISGTVTDASGRILSGQT
VEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDELPADTSGLLKEFAQAGLVN
IAGGCCGTTPEHIAAIAQALAGVKPRVWANYRETDTETV
>Mature_359_residues
MNQPAQTATPVRPDAAYTRGAALPALLKSRILILDGAMGTMIQRYKLDEARYRGERFKDYGRDIKGNNELLSITQPQIIS
EIHEQYLAAGADIVETNTFGATTVAQADYGMEALAVEMNLESAKLARAACDKYSTPDKPRFVAGAIGPTPKTASISPDVN
DPGARNVTFDELRAAYYEQARALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMISGTVTDASGRILSGQT
VEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDELPADTSGLLKEFAQAGLVN
IAGGCCGTTPEHIAAIAQALAGVKPRVWANYRETDTETV

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=324, Percent_Identity=59.8765432098765, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1790450, Length=327, Percent_Identity=60.5504587155963, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1786456, Length=326, Percent_Identity=28.8343558282209, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17535405, Length=327, Percent_Identity=56.5749235474006, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24585077, Length=289, Percent_Identity=24.2214532871972, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38582; Mature: 38582

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPAQTATPVRPDAAYTRGAALPALLKSRILILDGAMGTMIQRYKLDEARYRGERFKDY
CCCCCCCCCCCCCCHHHHCCHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
GRDIKGNNELLSITQPQIISEIHEQYLAAGADIVETNTFGATTVAQADYGMEALAVEMNL
CCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHCCCCCEEEEEEECC
ESAKLARAACDKYSTPDKPRFVAGAIGPTPKTASISPDVNDPGARNVTFDELRAAYYEQA
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMISGTVTDASGRILSGQT
HHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECCCH
VEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFD
HHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
ELPADTSGLLKEFAQAGLVNIAGGCCGTTPEHIAAIAQALAGVKPRVWANYRETDTETV
CCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure
MNQPAQTATPVRPDAAYTRGAALPALLKSRILILDGAMGTMIQRYKLDEARYRGERFKDY
CCCCCCCCCCCCCCHHHHCCHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
GRDIKGNNELLSITQPQIISEIHEQYLAAGADIVETNTFGATTVAQADYGMEALAVEMNL
CCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHCCCCCEEEEEEECC
ESAKLARAACDKYSTPDKPRFVAGAIGPTPKTASISPDVNDPGARNVTFDELRAAYYEQA
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMISGTVTDASGRILSGQT
HHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECCCH
VEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFD
HHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
ELPADTSGLLKEFAQAGLVNIAGGCCGTTPEHIAAIAQALAGVKPRVWANYRETDTETV
CCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]