| Definition | Burkholderia xenovorans LB400 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_007951 |
| Length | 4,895,836 |
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The map label for this gene is metH [H]
Identifier: 91781603
GI number: 91781603
Start: 254162
End: 256879
Strand: Reverse
Name: metH [H]
Synonym: Bxe_A4243
Alternate gene names: 91781603
Gene position: 256879-254162 (Counterclockwise)
Preceding gene: 91781604
Following gene: 91781601
Centisome position: 5.25
GC content: 62.36
Gene sequence:
>2718_bases ATGACCGATCACACCTTGCGCCTTGCCGGCCTCGAGCCGTTCAACGTCACGTCCGGGACGCTCTTCATCAACGTGGGCGA ACGCACCAACGTGACCGGCTCGAAGGCGTTTGCGCGAATGATCCTGAACGACCAGTTCGACGATGCGATCGCGGTCGCGC GCCAGCAGGTCGAAAACGGCGCGCAGATCATCGACGTCAACATGGACGAAGCGATGCTCGATTCGAAAGCGGCGATGGTG CGCTTCATGAATCTGATCGCGTCGGAACCGGATATCGCGCGCGTGCCGATCATGATCGACTCGTCGAAGTGGGAAGTGAT CGAAGCGGGCCTGAAGTGCGTGCAGGGCAAGGCCATCGTCAACTCGATCTCGCTGAAGGAAGGCGAAGAAGCGTTCCGCC ACCACGCGAACCTGATTCGCCGCTACGGCGCGGCCGCCGTGGTGATGGCCTTCGACGAACAGGGCCAGGCCGACACCTTC GAGCGCAAGACGCAGATCTGCAAGCGCTCGTATGACTTCCTCGTCAACGAAGTCGGCTTCCCGCCGGAAGACATCATCTT CGATCCGAACATCTTCGCGATCGCGACCGGCATCGAAGAGCACAACAACTACGCGGTCGACTTCATCAACGCAACGCGCT GGATCAAGCAGAATCTGCCGTACGCGAAGGTGAGCGGCGGCGTGTCCAACGTGTCGTTCTCGTTTCGCGGCAATGATCCG GTGCGCGAAGCGATCCACACCGTGTTCCTCTACCACGCGATCCAGGCGGGCATGGACATGGGCATCGTCAACGCGGGCCA GCTCGGCGTGTATGCCGACCTCGATCCGGAGTTGCGCGAACGCGTGGAAGACGTCGTGCTGAACCGCCGTGAAGACGGCA CCGACCGGCTGCTCGAAATCGCCGACAAGTTCAAGACCGGCGCCGCGAAGAAGGAAGAGAATCTGGAGTGGCGCAACCAG CCGGTCGAAAAACGTCTGTCGCATGCGCTCGTGCATGGCATCACCAACTTCATCGTCGAAGACACCGAGGAAGTGCGTGC GAAGATCGCCGCCGAAGGCGGCCGTCCGATCAATGTGATCGAAGGCCCGCTGATGGACGGCATGAACATCGTCGGCGACC TGTTCGGGCAGGGCAAGATGTTCCTGCCGCAAGTGGTGAAGTCGGCGCGCGTCATGAAGCAGGCGGTCGCGCATCTGATT CCGTATATCGAGGAAGAGAAGAAGCAGCTCGCCGAAGCCGGCGCCGACGTGCGTGCGAAGGGCAAGATCGTCATCGCCAC GGTGAAGGGCGACGTGCACGATATCGGCAAGAACATCGTCTCCGTGGTGCTTCAGTGCAATAACTTCGAAGTGGTCAACA TGGGCGTGATGGTCTCGTGCAACGACATTCTCGCCAAGGCGAAGGTCGAAGGCGCGGACATCATCGGGCTCTCCGGGTTG ATCACGCCGAGCCTCGAAGAGATGGCGTACGTCGCATCGGAAATGCAGCGCGACGACTACTTCCGCATCAAGAAGATTCC GCTGCTGATCGGCGGCGCCACCACCTCGCGCGTGCACACCGCGGTGAAGATCGCGCCGCATTACGAAGGGCCGGTGGTCT ACGTGCCGGACGCGTCGCGCTCGGTTTCCGTCGCGTCGAGCCTGCTGTCCGACGAGGGCGCGGCGAAGTACCTCGACGAA CTCAAGTCCGATTACGACCGGATCCGCGACCAGCACGCCAACAAGAAAGCACTGCCTATGGTCACGCTGGCCGAAGCCCG CGCCAACAAGACCAGGGTCGACTGGGCGAGCTATCAGCCGGTCAAGCCGAAGTTCATCGGCCGCCGCGTGTTCAGGAACT TCGATCTGGCCGAACTGGCGACCTATATCGACTGGGGTCCGTTCTTCCAGACGTGGGACCTGGCGGGCCCCTATCCGGCC ATCCTGAACGACGAGATCGTCGGCGAATCGGCGCGGCGCGTGTTCTCGGACGGCAAGTCGATGCTCGCGCGTCTGATCCA GGGCCGCTGGCTCCAGGCGAACGGCGTGATCGCGCTACTGCCCGCCAACACGGTGAACGACGACGACATCGAAATCTACA CGGACGAATCGCGCTCGGAAGTCGCGCTCACGTGGCGCAATCTGCGCCAGCAAAGCGTGCGTCCGGTGGTGGACGGCGTG ATGCGGCCGAACCGGTCGCTCGCTGACTTCATCGCGCCGAAAGACTCGGGCGTGGCCGACTACATCGGCATGTTCGCGGT GACGGCGGGACTCGGCGTCGACGTGAAGGAAAAGCAGTTCGAAAAAGATCACGACGACTACAGCGCGATCATGCTGAAGG CGCTCGCCGACCGTTTCGCCGAAGCCTTCGCCGAAGCGCTGCACGCCCGCGTGCGGCGCGACCTGTGGGGTTACGCAAAT GACGAAACGCTCTCCACCGACGAACTGATCGCGGAAAAGTACCGCGGCATCCGTCCGGCGCCCGGCTATCCGGCCTGCCC GGATCACCTGGTGAAGCGCGACATGTTCAATGTGCTGCACGCCACCGAAATCGGCATGAGCGTGACCGAATCGCTGGCCA TGCTGCCGGCGGCGAGCGTCTCCGGCTTCTATCTGGCACATCCGGACAGCACCTATTTCTCGGTCGGCAAGATCGGCCAG GACCAGCTGGAAGACTACGCGAGGCGCATGTCGCTCTCGAAAACGGATGCGGAGCGAGCCCTCGCGCCTTTGCTGTAA
Upstream 100 bases:
>100_bases CGCGAACAATGCCCGCACTTCCGCCAATGGTACGACCGCATCGTGACGCTGCCTGCCCTGCAGGCGCAGCAACCCAATAT TGAATATACGAGCTAACGCC
Downstream 100 bases:
>100_bases GCGCCGGGCGGCGAGGCGTGGCGGGAAACCCACTATATCGGGCATGAAAGACTGAATATTTGCGGCAACGTAATTTTCGG CTTTGTAGACTGAGGCGGCT
Product: methionine synthase (B12-dependent)
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 905; Mature: 904
Protein sequence:
>905_residues MTDHTLRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNDQFDDAIAVARQQVENGAQIIDVNMDEAMLDSKAAMV RFMNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEAFRHHANLIRRYGAAAVVMAFDEQGQADTF ERKTQICKRSYDFLVNEVGFPPEDIIFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPYAKVSGGVSNVSFSFRGNDP VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVVLNRREDGTDRLLEIADKFKTGAAKKEENLEWRNQ PVEKRLSHALVHGITNFIVEDTEEVRAKIAAEGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLI PYIEEEKKQLAEAGADVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVSCNDILAKAKVEGADIIGLSGL ITPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGAAKYLDE LKSDYDRIRDQHANKKALPMVTLAEARANKTRVDWASYQPVKPKFIGRRVFRNFDLAELATYIDWGPFFQTWDLAGPYPA ILNDEIVGESARRVFSDGKSMLARLIQGRWLQANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNLRQQSVRPVVDGV MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDVKEKQFEKDHDDYSAIMLKALADRFAEAFAEALHARVRRDLWGYAN DETLSTDELIAEKYRGIRPAPGYPACPDHLVKRDMFNVLHATEIGMSVTESLAMLPAASVSGFYLAHPDSTYFSVGKIGQ DQLEDYARRMSLSKTDAERALAPLL
Sequences:
>Translated_905_residues MTDHTLRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNDQFDDAIAVARQQVENGAQIIDVNMDEAMLDSKAAMV RFMNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEAFRHHANLIRRYGAAAVVMAFDEQGQADTF ERKTQICKRSYDFLVNEVGFPPEDIIFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPYAKVSGGVSNVSFSFRGNDP VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVVLNRREDGTDRLLEIADKFKTGAAKKEENLEWRNQ PVEKRLSHALVHGITNFIVEDTEEVRAKIAAEGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLI PYIEEEKKQLAEAGADVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVSCNDILAKAKVEGADIIGLSGL ITPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGAAKYLDE LKSDYDRIRDQHANKKALPMVTLAEARANKTRVDWASYQPVKPKFIGRRVFRNFDLAELATYIDWGPFFQTWDLAGPYPA ILNDEIVGESARRVFSDGKSMLARLIQGRWLQANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNLRQQSVRPVVDGV MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDVKEKQFEKDHDDYSAIMLKALADRFAEAFAEALHARVRRDLWGYAN DETLSTDELIAEKYRGIRPAPGYPACPDHLVKRDMFNVLHATEIGMSVTESLAMLPAASVSGFYLAHPDSTYFSVGKIGQ DQLEDYARRMSLSKTDAERALAPLL >Mature_904_residues TDHTLRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNDQFDDAIAVARQQVENGAQIIDVNMDEAMLDSKAAMVR FMNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEAFRHHANLIRRYGAAAVVMAFDEQGQADTFE RKTQICKRSYDFLVNEVGFPPEDIIFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPYAKVSGGVSNVSFSFRGNDPV REAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVVLNRREDGTDRLLEIADKFKTGAAKKEENLEWRNQP VEKRLSHALVHGITNFIVEDTEEVRAKIAAEGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIP YIEEEKKQLAEAGADVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVSCNDILAKAKVEGADIIGLSGLI TPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGAAKYLDEL KSDYDRIRDQHANKKALPMVTLAEARANKTRVDWASYQPVKPKFIGRRVFRNFDLAELATYIDWGPFFQTWDLAGPYPAI LNDEIVGESARRVFSDGKSMLARLIQGRWLQANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNLRQQSVRPVVDGVM RPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDVKEKQFEKDHDDYSAIMLKALADRFAEAFAEALHARVRRDLWGYAND ETLSTDELIAEKYRGIRPAPGYPACPDHLVKRDMFNVLHATEIGMSVTESLAMLPAASVSGFYLAHPDSTYFSVGKIGQD QLEDYARRMSLSKTDAERALAPLL
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=918, Percent_Identity=53.4858387799564, Blast_Score=961, Evalue=0.0, Organism=Escherichia coli, GI1790450, Length=905, Percent_Identity=61.4364640883978, Blast_Score=1092, Evalue=0.0, Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.1666666666667, Blast_Score=944, Evalue=0.0,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 100208; Mature: 100077
Theoretical pI: Translated: 5.19; Mature: 5.19
Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTDHTLRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNDQFDDAIAVARQQVENG CCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCC AQIIDVNMDEAMLDSKAAMVRFMNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH NSISLKEGEEAFRHHANLIRRYGAAAVVMAFDEQGQADTFERKTQICKRSYDFLVNEVGF HHCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC PPEDIIFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPYAKVSGGVSNVSFSFRGNDP CCHHEEECCCEEEEEECCHHCCCEEEEEHHHHHHHHHCCCCEEECCCCCEEEEEECCCCH VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVVLNRREDGTDRLLEI HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH ADKFKTGAAKKEENLEWRNQPVEKRLSHALVHGITNFIVEDTEEVRAKIAAEGGRPINVI HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEE EGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPYIEEEKKQLAEAGADVRAK CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVSCNDILAKAKVEGADIIGLSGL CCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHHCCCCCEEEECCC ITPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASR CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCC SVSVASSLLSDEGAAKYLDELKSDYDRIRDQHANKKALPMVTLAEARANKTRVDWASYQP HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCCCCCCCCC VKPKFIGRRVFRNFDLAELATYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDGKS CCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHCHHHHHHHHCHHH MLARLIQGRWLQANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNLRQQSVRPVVDGV HHHHHHCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDVKEKQFEKDHDDYSAIMLKALADRFA HCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH EAFAEALHARVRRDLWGYANDETLSTDELIAEKYRGIRPAPGYPACPDHLVKRDMFNVLH HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH ATEIGMSVTESLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLEDYARRMSLSKTDAERA HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHH LAPLL HHCCC >Mature Secondary Structure TDHTLRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNDQFDDAIAVARQQVENG CCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCC AQIIDVNMDEAMLDSKAAMVRFMNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH NSISLKEGEEAFRHHANLIRRYGAAAVVMAFDEQGQADTFERKTQICKRSYDFLVNEVGF HHCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC PPEDIIFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPYAKVSGGVSNVSFSFRGNDP CCHHEEECCCEEEEEECCHHCCCEEEEEHHHHHHHHHCCCCEEECCCCCEEEEEECCCCH VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVVLNRREDGTDRLLEI HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH ADKFKTGAAKKEENLEWRNQPVEKRLSHALVHGITNFIVEDTEEVRAKIAAEGGRPINVI HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEE EGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPYIEEEKKQLAEAGADVRAK CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVSCNDILAKAKVEGADIIGLSGL CCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHHCCCCCEEEECCC ITPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASR CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCC SVSVASSLLSDEGAAKYLDELKSDYDRIRDQHANKKALPMVTLAEARANKTRVDWASYQP HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCCCCCCCCC VKPKFIGRRVFRNFDLAELATYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDGKS CCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHCHHHHHHHHCHHH MLARLIQGRWLQANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNLRQQSVRPVVDGV HHHHHHCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDVKEKQFEKDHDDYSAIMLKALADRFA HCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH EAFAEALHARVRRDLWGYANDETLSTDELIAEKYRGIRPAPGYPACPDHLVKRDMFNVLH HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH ATEIGMSVTESLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLEDYARRMSLSKTDAERA HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHH LAPLL HHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]