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The map label for this gene is gatA [H]

Identifier: 91781450

GI number: 91781450

Start: 82586

End: 84073

Strand: Direct

Name: gatA [H]

Synonym: Bxe_A4396

Alternate gene names: 91781450

Gene position: 82586-84073 (Clockwise)

Preceding gene: 91781449

Following gene: 91781451

Centisome position: 1.69

GC content: 65.39

Gene sequence:

>1488_bases
ATGCATGAAAAAAGTCTGACCGAACTGCGCGCCGCCTTGACGGCGAGGGAATGCTCCGCAGTCGAACTGGCGCAGCTCTA
TCTGAAGCGGATCGAAGCGGCCAACAGCCTGAACGCATTCATCCAGGTCGACCCCGATCTGACGCTCGCGCAGGCAAAAG
CCGCGGACGCGCTGCTCCATACCGGCCACGCCGGCCCGCTGGTCGGCCTGCCGATCGCGCACAAGGACGTGTTCGTCACC
AAAGGCTGGCGCTCCACGGCCGGCTCGAAAATGCTGTCCAACTACGAGAGTCCGTTCGACGCGACCGTGGTCGCGCGTCT
GCAGAACGCCGGCATGGTGTGCGTGGGCAAGACCAACATGGACGAGTTCGCAATGGGCTCGTCCAACGAGAATTCGTACT
TTGGTCCCGTGCAGAATCCGTGGGACGTCAAGGCCGTGCCGGGCGGTTCGTCGGGCGGTTCGGCGGCGGCCGTGGCCGCG
CGCCTCGCGCCCGCTGCAACCGGCACGGATACCGGCGGTTCGATCCGTCAGCCGGCGTCGTTTTCCGGCATCACCGGCAT
CAAACCCACGTATGGCCGCGTGTCGCGTTACGGCATGATCGCGTTCGCCTCGTCGCTGGATCAGGGCGGGCCGATGGCGC
GAAGCGCCGCGGATTGCGCCACGCTGCTCAACGCAATGGCCGGTTTCGACGGGCGTGATTCGACCAGCCTCGTGCGTGAC
GACGAAGACTACACGCGCTACCTCGGCCAGCCGTGGAAAGAAGACGGCGCCGGCAAACCGCTCGCCGGCCTGCGCATCGG
CCTGCCGAAAGAGTATTTCGGCGCCGGTCTCGCCGACGACGTGCGTGCCTCGATCGACGCCGCGCTCAAGCAATACGAAG
CGCTCGGCGCCACGCTGGTCGAGGTTTCGCTGCCGAAAACGGAGTTGTCGATCCCGGTGTACTACGTGATCGCGCCGGCG
GAGGCTTCGTCGAACCTGTCGCGTTTCGACGGCGTGCGCTTCGGCCATCGCGCCGCGGAGTATCGCGATCTGCTCGACAT
GTACAAGAAGTCGCGCGCCGAAGGTTTCGGCCCGGAAGTGAAGCGCCGCATTCTGGTCGGCGCCTACGTGCTGTCGCACG
GCTATTACGACGCGTACTACCTGCAGGCGCAGAAGATCCGCCGCATTATCGCGCAGGACTTCCAGGAAGCGTTCAGACAG
TGCGACGTGATCATGGGGCCGGTCGCGCCAACGGTGGCGTGGGATCTCGGCGCGAAGGGCGACGATCCGGTGCAGATGTA
TCTGGCCGACATCTATACGCTGTCGGTGAGCCTCGCCGGTTTGCCGGGCATGAGCGTGCCGTGCGGCTTCGGCGCGGGCG
CGAATGCGCAGCGTCCGGTAGGTCTGCAGATCATCGGCAACTATTTCAATGAAGCCCGGATGCTGCAGGTGGCCGACGCG
TTCCAGCGCGCGACCGACTGGCACCGCAAAGCACCGGCAGGAGTGTGA

Upstream 100 bases:

>100_bases
GACCGTCGTCTCCGGCTTTCTCGGCTCGGCTTGCCGGCGGGCAGCCGAATGCCTCCACGGCAGCGTGACTGCGCTGCCGC
GCTCTTCAGGATAAAACGCA

Downstream 100 bases:

>100_bases
GCACCATGACCAAGCAATGGGAAGTCGTGATCGGTCTGGAGACCCACGCGCAACTGTCGACCCACTCGAAGATTTTTTCG
GGCGCCTCGACGCAATTCGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MHEKSLTELRAALTARECSAVELAQLYLKRIEAANSLNAFIQVDPDLTLAQAKAADALLHTGHAGPLVGLPIAHKDVFVT
KGWRSTAGSKMLSNYESPFDATVVARLQNAGMVCVGKTNMDEFAMGSSNENSYFGPVQNPWDVKAVPGGSSGGSAAAVAA
RLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARSAADCATLLNAMAGFDGRDSTSLVRD
DEDYTRYLGQPWKEDGAGKPLAGLRIGLPKEYFGAGLADDVRASIDAALKQYEALGATLVEVSLPKTELSIPVYYVIAPA
EASSNLSRFDGVRFGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGAYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFRQ
CDVIMGPVAPTVAWDLGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAQRPVGLQIIGNYFNEARMLQVADA
FQRATDWHRKAPAGV

Sequences:

>Translated_495_residues
MHEKSLTELRAALTARECSAVELAQLYLKRIEAANSLNAFIQVDPDLTLAQAKAADALLHTGHAGPLVGLPIAHKDVFVT
KGWRSTAGSKMLSNYESPFDATVVARLQNAGMVCVGKTNMDEFAMGSSNENSYFGPVQNPWDVKAVPGGSSGGSAAAVAA
RLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARSAADCATLLNAMAGFDGRDSTSLVRD
DEDYTRYLGQPWKEDGAGKPLAGLRIGLPKEYFGAGLADDVRASIDAALKQYEALGATLVEVSLPKTELSIPVYYVIAPA
EASSNLSRFDGVRFGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGAYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFRQ
CDVIMGPVAPTVAWDLGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAQRPVGLQIIGNYFNEARMLQVADA
FQRATDWHRKAPAGV
>Mature_495_residues
MHEKSLTELRAALTARECSAVELAQLYLKRIEAANSLNAFIQVDPDLTLAQAKAADALLHTGHAGPLVGLPIAHKDVFVT
KGWRSTAGSKMLSNYESPFDATVVARLQNAGMVCVGKTNMDEFAMGSSNENSYFGPVQNPWDVKAVPGGSSGGSAAAVAA
RLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARSAADCATLLNAMAGFDGRDSTSLVRD
DEDYTRYLGQPWKEDGAGKPLAGLRIGLPKEYFGAGLADDVRASIDAALKQYEALGATLVEVSLPKTELSIPVYYVIAPA
EASSNLSRFDGVRFGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGAYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFRQ
CDVIMGPVAPTVAWDLGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAQRPVGLQIIGNYFNEARMLQVADA
FQRATDWHRKAPAGV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=511, Percent_Identity=37.3776908023483, Blast_Score=315, Evalue=8e-86,
Organism=Homo sapiens, GI195972892, Length=500, Percent_Identity=25.6, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI166795287, Length=157, Percent_Identity=39.4904458598726, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=455, Percent_Identity=36.9230769230769, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17537465, Length=513, Percent_Identity=22.4171539961014, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=30.7291666666667, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=228, Percent_Identity=32.4561403508772, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=231, Percent_Identity=29.004329004329, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=190, Percent_Identity=31.0526315789474, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=190, Percent_Identity=31.0526315789474, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=198, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=442, Percent_Identity=34.841628959276, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=419, Percent_Identity=29.3556085918854, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=545, Percent_Identity=22.7522935779817, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24648113, Length=459, Percent_Identity=40.5228758169935, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24652985, Length=508, Percent_Identity=26.7716535433071, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922090, Length=508, Percent_Identity=26.7716535433071, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24652981, Length=508, Percent_Identity=26.7716535433071, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24652983, Length=508, Percent_Identity=26.7716535433071, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI161078093, Length=521, Percent_Identity=26.679462571977, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24644968, Length=512, Percent_Identity=25, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45550774, Length=510, Percent_Identity=24.9019607843137, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648435, Length=510, Percent_Identity=24.9019607843137, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648437, Length=510, Percent_Identity=24.9019607843137, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21356731, Length=237, Percent_Identity=31.6455696202532, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648441, Length=442, Percent_Identity=25.5656108597285, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648439, Length=442, Percent_Identity=25.5656108597285, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52845; Mature: 52845

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEKSLTELRAALTARECSAVELAQLYLKRIEAANSLNAFIQVDPDLTLAQAKAADALLH
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHH
TGHAGPLVGLPIAHKDVFVTKGWRSTAGSKMLSNYESPFDATVVARLQNAGMVCVGKTNM
CCCCCCEEECCCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCC
DEFAMGSSNENSYFGPVQNPWDVKAVPGGSSGGSAAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCEECCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
FSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARSAADCATLLNAMAGFDGRDSTSLVRD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DEDYTRYLGQPWKEDGAGKPLAGLRIGLPKEYFGAGLADDVRASIDAALKQYEALGATLV
CHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEE
EVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRFGHRAAEYRDLLDMYKKSRAEGFGPEV
EEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KRRILVGAYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFRQCDVIMGPVAPTVAWDLGAKG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHEEECCCCC
DDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAQRPVGLQIIGNYFNEARMLQVADA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FQRATDWHRKAPAGV
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MHEKSLTELRAALTARECSAVELAQLYLKRIEAANSLNAFIQVDPDLTLAQAKAADALLH
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHH
TGHAGPLVGLPIAHKDVFVTKGWRSTAGSKMLSNYESPFDATVVARLQNAGMVCVGKTNM
CCCCCCEEECCCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCC
DEFAMGSSNENSYFGPVQNPWDVKAVPGGSSGGSAAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCEECCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
FSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARSAADCATLLNAMAGFDGRDSTSLVRD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DEDYTRYLGQPWKEDGAGKPLAGLRIGLPKEYFGAGLADDVRASIDAALKQYEALGATLV
CHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEE
EVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRFGHRAAEYRDLLDMYKKSRAEGFGPEV
EEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KRRILVGAYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFRQCDVIMGPVAPTVAWDLGAKG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHEEECCCCC
DDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAQRPVGLQIIGNYFNEARMLQVADA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FQRATDWHRKAPAGV
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA